Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4677 and Pf6N2E2_4678 overlap by 9 nucleotides Pf6N2E2_4678 and Pf6N2E2_4679 overlap by 19 nucleotides
Pf6N2E2_4677 - Choline dehydrogenase (EC 1.1.99.1), at 5,410,297 to 5,412,000
_4677
Pf6N2E2_4678 - hypothetical protein, at 5,411,992 to 5,412,231
_4678
Pf6N2E2_4679 - Betaine aldehyde dehydrogenase (EC 1.2.1.8), at 5,412,213 to 5,413,685
_4679
Position (kb)
5411
5412
5413 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5411.033 kb on + strand, within Pf6N2E2_4677 at 5411.052 kb on - strand, within Pf6N2E2_4677 at 5411.078 kb on + strand, within Pf6N2E2_4677 at 5411.078 kb on + strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.079 kb on - strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.087 kb on + strand, within Pf6N2E2_4677 at 5411.088 kb on - strand, within Pf6N2E2_4677 at 5411.088 kb on - strand, within Pf6N2E2_4677 at 5411.384 kb on + strand, within Pf6N2E2_4677 at 5411.385 kb on - strand, within Pf6N2E2_4677 at 5411.497 kb on + strand, within Pf6N2E2_4677 at 5411.498 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.684 kb on + strand, within Pf6N2E2_4677 at 5411.685 kb on - strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.805 kb on - strand, within Pf6N2E2_4677 at 5411.981 kb on + strand at 5411.981 kb on + strand at 5411.982 kb on - strand at 5411.989 kb on + strand at 5411.989 kb on + strand at 5411.990 kb on - strand at 5412.099 kb on - strand, within Pf6N2E2_4678 at 5412.249 kb on + strand at 5412.319 kb on + strand at 5412.375 kb on - strand, within Pf6N2E2_4679 at 5412.493 kb on + strand, within Pf6N2E2_4679 at 5412.494 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5413.111 kb on + strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,411,033 + Pf6N2E2_4677 0.43 +0.8 5,411,052 - Pf6N2E2_4677 0.44 +0.5 5,411,078 + Pf6N2E2_4677 0.46 -0.2 5,411,078 + Pf6N2E2_4677 0.46 +0.6 5,411,079 - Pf6N2E2_4677 0.46 -1.7 5,411,079 - Pf6N2E2_4677 0.46 +0.4 5,411,079 - Pf6N2E2_4677 0.46 -1.3 5,411,087 + Pf6N2E2_4677 0.46 -0.4 5,411,087 + Pf6N2E2_4677 0.46 -0.9 5,411,087 + Pf6N2E2_4677 0.46 -1.1 5,411,087 + Pf6N2E2_4677 0.46 -1.8 5,411,088 - Pf6N2E2_4677 0.46 -2.1 5,411,088 - Pf6N2E2_4677 0.46 -0.4 5,411,384 + Pf6N2E2_4677 0.64 -1.4 5,411,385 - Pf6N2E2_4677 0.64 +0.1 5,411,497 + Pf6N2E2_4677 0.70 -2.6 5,411,498 - Pf6N2E2_4677 0.70 -0.3 5,411,625 - Pf6N2E2_4677 0.78 +0.7 5,411,625 - Pf6N2E2_4677 0.78 -0.6 5,411,642 + Pf6N2E2_4677 0.79 -0.6 5,411,642 + Pf6N2E2_4677 0.79 -1.1 5,411,643 - Pf6N2E2_4677 0.79 -1.9 5,411,643 - Pf6N2E2_4677 0.79 +0.3 5,411,643 - Pf6N2E2_4677 0.79 -0.1 5,411,684 + Pf6N2E2_4677 0.81 -1.7 5,411,685 - Pf6N2E2_4677 0.81 -2.2 5,411,708 + Pf6N2E2_4677 0.83 -0.5 5,411,708 + Pf6N2E2_4677 0.83 +0.3 5,411,708 + Pf6N2E2_4677 0.83 -1.9 5,411,708 + Pf6N2E2_4677 0.83 +0.2 5,411,708 + Pf6N2E2_4677 0.83 -0.0 5,411,708 + Pf6N2E2_4677 0.83 -1.5 5,411,708 + Pf6N2E2_4677 0.83 -0.2 5,411,708 + Pf6N2E2_4677 0.83 -0.3 5,411,709 - Pf6N2E2_4677 0.83 +1.4 5,411,709 - Pf6N2E2_4677 0.83 +0.1 5,411,709 - Pf6N2E2_4677 0.83 +1.6 5,411,709 - Pf6N2E2_4677 0.83 -0.9 5,411,709 - Pf6N2E2_4677 0.83 +1.9 5,411,805 - Pf6N2E2_4677 0.88 -0.8 5,411,981 + -1.9 5,411,981 + -0.4 5,411,982 - -0.4 5,411,989 + -1.5 5,411,989 + -1.0 5,411,990 - +0.4 5,412,099 - Pf6N2E2_4678 0.45 -2.3 5,412,249 + -0.3 5,412,319 + -1.6 5,412,375 - Pf6N2E2_4679 0.11 +0.9 5,412,493 + Pf6N2E2_4679 0.19 +1.7 5,412,494 - Pf6N2E2_4679 0.19 -0.8 5,412,668 - Pf6N2E2_4679 0.31 +0.5 5,412,668 - Pf6N2E2_4679 0.31 -1.4 5,412,668 - Pf6N2E2_4679 0.31 -2.6 5,412,847 + Pf6N2E2_4679 0.43 -0.4 5,412,847 + Pf6N2E2_4679 0.43 -0.7 5,412,847 + Pf6N2E2_4679 0.43 -0.8 5,412,848 - Pf6N2E2_4679 0.43 -1.2 5,412,848 - Pf6N2E2_4679 0.43 +0.5 5,413,111 + Pf6N2E2_4679 0.61 -1.6 5,413,112 - Pf6N2E2_4679 0.61 -2.7 5,413,112 - Pf6N2E2_4679 0.61 -0.8 5,413,112 - Pf6N2E2_4679 0.61 +1.6
Or see this region's nucleotide sequence