Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4531 and Pf6N2E2_4532 are separated by 2 nucleotides Pf6N2E2_4532 and Pf6N2E2_4533 overlap by 14 nucleotides Pf6N2E2_4533 and Pf6N2E2_4534 are separated by 115 nucleotides Pf6N2E2_4534 and Pf6N2E2_4535 overlap by 4 nucleotides
Pf6N2E2_4531 - hypothetical protein, at 5,266,100 to 5,266,993
_4531
Pf6N2E2_4532 - Flagellum-specific ATP synthase FliI, at 5,266,996 to 5,268,351
_4532
Pf6N2E2_4533 - RspO, at 5,268,338 to 5,268,757
_4533
Pf6N2E2_4534 - hypothetical protein, at 5,268,873 to 5,269,319
_4534
Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419
_4535
Position (kb) 5266
5267
5268
5269 Strain fitness (log2 ratio) -2
-1
0
1 at 5266.053 kb on - strand at 5266.053 kb on - strand at 5266.206 kb on - strand, within Pf6N2E2_4531 at 5266.977 kb on - strand at 5266.977 kb on - strand at 5267.513 kb on + strand, within Pf6N2E2_4532 at 5267.514 kb on - strand, within Pf6N2E2_4532 at 5267.885 kb on - strand, within Pf6N2E2_4532 at 5267.950 kb on + strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.952 kb on + strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5268.003 kb on - strand, within Pf6N2E2_4532 at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.338 kb on - strand at 5268.728 kb on + strand at 5268.832 kb on - strand at 5268.939 kb on + strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,266,053 - +0.9 5,266,053 - +1.4 5,266,206 - Pf6N2E2_4531 0.12 +0.0 5,266,977 - -0.6 5,266,977 - +1.5 5,267,513 + Pf6N2E2_4532 0.38 -0.3 5,267,514 - Pf6N2E2_4532 0.38 -0.9 5,267,885 - Pf6N2E2_4532 0.66 +0.4 5,267,950 + Pf6N2E2_4532 0.70 +0.6 5,267,951 - Pf6N2E2_4532 0.70 +1.0 5,267,951 - Pf6N2E2_4532 0.70 -0.2 5,267,952 + Pf6N2E2_4532 0.71 -0.6 5,267,953 - Pf6N2E2_4532 0.71 +0.1 5,267,953 - Pf6N2E2_4532 0.71 -0.0 5,267,953 - Pf6N2E2_4532 0.71 +0.2 5,267,953 - Pf6N2E2_4532 0.71 -0.5 5,268,003 - Pf6N2E2_4532 0.74 -0.9 5,268,337 + +0.0 5,268,337 + +1.3 5,268,337 + -1.9 5,268,338 - +0.8 5,268,728 + +0.8 5,268,832 - +0.6 5,268,939 + Pf6N2E2_4534 0.15 -0.3 5,269,208 - Pf6N2E2_4534 0.75 +0.6 5,269,208 - Pf6N2E2_4534 0.75 -1.2 5,269,208 - Pf6N2E2_4534 0.75 +0.7
Or see this region's nucleotide sequence