Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4469 and Pf6N2E2_4470 are separated by 117 nucleotides Pf6N2E2_4470 and Pf6N2E2_4471 are separated by 67 nucleotides
Pf6N2E2_4469 - Quinone oxidoreductase (EC 1.6.5.5), at 5,201,818 to 5,202,795
_4469
Pf6N2E2_4470 - Flagellar biosynthesis protein FliL, at 5,202,913 to 5,203,362
_4470
Pf6N2E2_4471 - ABC-type multidrug transport system, permease component, at 5,203,430 to 5,204,548
_4471
Position (kb)
5202
5203
5204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5202.159 kb on - strand, within Pf6N2E2_4469 at 5202.159 kb on - strand, within Pf6N2E2_4469 at 5202.224 kb on + strand, within Pf6N2E2_4469 at 5202.225 kb on - strand, within Pf6N2E2_4469 at 5202.225 kb on - strand, within Pf6N2E2_4469 at 5202.425 kb on + strand, within Pf6N2E2_4469 at 5202.425 kb on + strand, within Pf6N2E2_4469 at 5202.425 kb on + strand, within Pf6N2E2_4469 at 5202.425 kb on + strand, within Pf6N2E2_4469 at 5202.425 kb on + strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.426 kb on - strand, within Pf6N2E2_4469 at 5202.488 kb on + strand, within Pf6N2E2_4469 at 5202.644 kb on + strand, within Pf6N2E2_4469 at 5202.644 kb on + strand, within Pf6N2E2_4469 at 5202.644 kb on + strand, within Pf6N2E2_4469 at 5202.644 kb on + strand, within Pf6N2E2_4469 at 5202.645 kb on - strand, within Pf6N2E2_4469 at 5202.645 kb on - strand, within Pf6N2E2_4469 at 5202.645 kb on - strand, within Pf6N2E2_4469 at 5202.802 kb on + strand at 5202.803 kb on - strand at 5202.826 kb on + strand at 5202.826 kb on + strand at 5202.827 kb on - strand at 5202.832 kb on + strand at 5202.832 kb on + strand at 5202.832 kb on + strand at 5202.832 kb on + strand at 5202.832 kb on + strand at 5202.832 kb on + strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.833 kb on - strand at 5202.883 kb on + strand at 5202.883 kb on + strand at 5202.884 kb on - strand at 5202.884 kb on - strand at 5202.885 kb on + strand at 5202.885 kb on + strand at 5202.885 kb on + strand at 5202.885 kb on + strand at 5202.886 kb on - strand at 5202.886 kb on - strand at 5202.886 kb on - strand at 5202.886 kb on - strand at 5202.886 kb on - strand at 5202.913 kb on - strand at 5203.044 kb on + strand, within Pf6N2E2_4470 at 5203.045 kb on - strand, within Pf6N2E2_4470 at 5203.045 kb on - strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.048 kb on + strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.049 kb on - strand, within Pf6N2E2_4470 at 5203.081 kb on + strand, within Pf6N2E2_4470 at 5203.081 kb on + strand, within Pf6N2E2_4470 at 5203.081 kb on + strand, within Pf6N2E2_4470 at 5203.081 kb on + strand, within Pf6N2E2_4470 at 5203.081 kb on + strand, within Pf6N2E2_4470 at 5203.082 kb on - strand, within Pf6N2E2_4470 at 5203.082 kb on - strand, within Pf6N2E2_4470 at 5203.082 kb on - strand, within Pf6N2E2_4470 at 5203.082 kb on - strand, within Pf6N2E2_4470 at 5203.114 kb on + strand, within Pf6N2E2_4470 at 5203.114 kb on + strand, within Pf6N2E2_4470 at 5203.114 kb on + strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.115 kb on - strand, within Pf6N2E2_4470 at 5203.569 kb on + strand, within Pf6N2E2_4471 at 5203.569 kb on + strand, within Pf6N2E2_4471 at 5203.569 kb on + strand, within Pf6N2E2_4471 at 5203.569 kb on + strand, within Pf6N2E2_4471 at 5203.569 kb on + strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.570 kb on - strand, within Pf6N2E2_4471 at 5203.633 kb on + strand, within Pf6N2E2_4471 at 5203.752 kb on + strand, within Pf6N2E2_4471 at 5203.752 kb on + strand, within Pf6N2E2_4471 at 5203.752 kb on + strand, within Pf6N2E2_4471 at 5203.752 kb on + strand, within Pf6N2E2_4471 at 5203.752 kb on + strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5203.753 kb on - strand, within Pf6N2E2_4471 at 5204.044 kb on + strand, within Pf6N2E2_4471 at 5204.045 kb on - strand, within Pf6N2E2_4471 at 5204.109 kb on + strand, within Pf6N2E2_4471 at 5204.109 kb on + strand, within Pf6N2E2_4471 at 5204.109 kb on + strand, within Pf6N2E2_4471 at 5204.109 kb on + strand, within Pf6N2E2_4471 at 5204.109 kb on + strand, within Pf6N2E2_4471 at 5204.110 kb on - strand, within Pf6N2E2_4471
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,202,159 - Pf6N2E2_4469 0.35 +2.3 5,202,159 - Pf6N2E2_4469 0.35 +1.6 5,202,224 + Pf6N2E2_4469 0.42 +0.7 5,202,225 - Pf6N2E2_4469 0.42 -1.2 5,202,225 - Pf6N2E2_4469 0.42 +0.1 5,202,425 + Pf6N2E2_4469 0.62 -2.0 5,202,425 + Pf6N2E2_4469 0.62 -2.4 5,202,425 + Pf6N2E2_4469 0.62 +0.7 5,202,425 + Pf6N2E2_4469 0.62 -0.1 5,202,425 + Pf6N2E2_4469 0.62 +1.6 5,202,426 - Pf6N2E2_4469 0.62 +2.8 5,202,426 - Pf6N2E2_4469 0.62 -0.6 5,202,426 - Pf6N2E2_4469 0.62 +0.3 5,202,426 - Pf6N2E2_4469 0.62 +2.1 5,202,426 - Pf6N2E2_4469 0.62 -0.8 5,202,426 - Pf6N2E2_4469 0.62 -2.1 5,202,426 - Pf6N2E2_4469 0.62 +0.4 5,202,426 - Pf6N2E2_4469 0.62 -0.3 5,202,488 + Pf6N2E2_4469 0.69 -0.3 5,202,644 + Pf6N2E2_4469 0.84 -0.6 5,202,644 + Pf6N2E2_4469 0.84 -0.5 5,202,644 + Pf6N2E2_4469 0.84 -0.7 5,202,644 + Pf6N2E2_4469 0.84 -1.0 5,202,645 - Pf6N2E2_4469 0.85 -0.8 5,202,645 - Pf6N2E2_4469 0.85 -1.5 5,202,645 - Pf6N2E2_4469 0.85 +1.8 5,202,802 + -0.9 5,202,803 - -0.7 5,202,826 + -1.4 5,202,826 + -0.9 5,202,827 - +0.5 5,202,832 + -1.3 5,202,832 + +1.9 5,202,832 + +0.2 5,202,832 + +0.1 5,202,832 + -0.6 5,202,832 + +0.1 5,202,833 - -0.5 5,202,833 - -2.3 5,202,833 - -0.9 5,202,833 - +0.4 5,202,833 - +0.1 5,202,833 - -0.3 5,202,833 - +0.5 5,202,833 - +1.2 5,202,883 + -0.1 5,202,883 + -0.1 5,202,884 - -0.5 5,202,884 - -0.6 5,202,885 + -0.1 5,202,885 + +0.4 5,202,885 + +1.0 5,202,885 + -1.2 5,202,886 - +0.3 5,202,886 - +0.1 5,202,886 - -0.3 5,202,886 - -0.6 5,202,886 - -0.9 5,202,913 - -0.3 5,203,044 + Pf6N2E2_4470 0.29 -0.5 5,203,045 - Pf6N2E2_4470 0.29 +1.0 5,203,045 - Pf6N2E2_4470 0.29 +0.4 5,203,048 + Pf6N2E2_4470 0.30 +0.2 5,203,048 + Pf6N2E2_4470 0.30 +0.7 5,203,048 + Pf6N2E2_4470 0.30 +1.5 5,203,048 + Pf6N2E2_4470 0.30 +0.5 5,203,048 + Pf6N2E2_4470 0.30 -0.2 5,203,048 + Pf6N2E2_4470 0.30 +0.6 5,203,048 + Pf6N2E2_4470 0.30 -0.4 5,203,048 + Pf6N2E2_4470 0.30 -0.5 5,203,048 + Pf6N2E2_4470 0.30 +0.6 5,203,048 + Pf6N2E2_4470 0.30 -0.1 5,203,048 + Pf6N2E2_4470 0.30 +1.5 5,203,049 - Pf6N2E2_4470 0.30 +0.9 5,203,049 - Pf6N2E2_4470 0.30 -0.1 5,203,049 - Pf6N2E2_4470 0.30 +1.3 5,203,049 - Pf6N2E2_4470 0.30 +1.3 5,203,049 - Pf6N2E2_4470 0.30 +1.6 5,203,049 - Pf6N2E2_4470 0.30 +0.8 5,203,049 - Pf6N2E2_4470 0.30 +1.5 5,203,049 - Pf6N2E2_4470 0.30 +0.7 5,203,049 - Pf6N2E2_4470 0.30 -1.5 5,203,049 - Pf6N2E2_4470 0.30 +0.7 5,203,081 + Pf6N2E2_4470 0.37 +0.9 5,203,081 + Pf6N2E2_4470 0.37 +0.6 5,203,081 + Pf6N2E2_4470 0.37 -0.2 5,203,081 + Pf6N2E2_4470 0.37 +1.7 5,203,081 + Pf6N2E2_4470 0.37 +2.2 5,203,082 - Pf6N2E2_4470 0.38 +2.1 5,203,082 - Pf6N2E2_4470 0.38 -0.9 5,203,082 - Pf6N2E2_4470 0.38 +0.2 5,203,082 - Pf6N2E2_4470 0.38 -2.2 5,203,114 + Pf6N2E2_4470 0.45 -1.5 5,203,114 + Pf6N2E2_4470 0.45 +3.0 5,203,114 + Pf6N2E2_4470 0.45 -0.8 5,203,115 - Pf6N2E2_4470 0.45 +0.8 5,203,115 - Pf6N2E2_4470 0.45 +2.6 5,203,115 - Pf6N2E2_4470 0.45 -2.8 5,203,115 - Pf6N2E2_4470 0.45 -0.4 5,203,115 - Pf6N2E2_4470 0.45 +3.2 5,203,115 - Pf6N2E2_4470 0.45 +0.7 5,203,569 + Pf6N2E2_4471 0.12 -0.7 5,203,569 + Pf6N2E2_4471 0.12 -1.2 5,203,569 + Pf6N2E2_4471 0.12 -0.0 5,203,569 + Pf6N2E2_4471 0.12 +0.9 5,203,569 + Pf6N2E2_4471 0.12 +0.1 5,203,570 - Pf6N2E2_4471 0.13 +0.7 5,203,570 - Pf6N2E2_4471 0.13 -0.5 5,203,570 - Pf6N2E2_4471 0.13 -0.2 5,203,570 - Pf6N2E2_4471 0.13 -0.7 5,203,570 - Pf6N2E2_4471 0.13 +0.3 5,203,570 - Pf6N2E2_4471 0.13 +0.3 5,203,570 - Pf6N2E2_4471 0.13 -0.5 5,203,570 - Pf6N2E2_4471 0.13 -1.5 5,203,570 - Pf6N2E2_4471 0.13 +0.2 5,203,633 + Pf6N2E2_4471 0.18 -0.3 5,203,752 + Pf6N2E2_4471 0.29 -2.7 5,203,752 + Pf6N2E2_4471 0.29 -1.4 5,203,752 + Pf6N2E2_4471 0.29 -1.7 5,203,752 + Pf6N2E2_4471 0.29 +1.4 5,203,752 + Pf6N2E2_4471 0.29 -1.3 5,203,753 - Pf6N2E2_4471 0.29 +0.1 5,203,753 - Pf6N2E2_4471 0.29 +0.1 5,203,753 - Pf6N2E2_4471 0.29 +1.4 5,203,753 - Pf6N2E2_4471 0.29 -0.5 5,203,753 - Pf6N2E2_4471 0.29 -0.6 5,203,753 - Pf6N2E2_4471 0.29 +0.4 5,204,044 + Pf6N2E2_4471 0.55 +0.5 5,204,045 - Pf6N2E2_4471 0.55 +1.2 5,204,109 + Pf6N2E2_4471 0.61 -1.2 5,204,109 + Pf6N2E2_4471 0.61 -0.0 5,204,109 + Pf6N2E2_4471 0.61 -1.0 5,204,109 + Pf6N2E2_4471 0.61 -2.5 5,204,109 + Pf6N2E2_4471 0.61 +0.6 5,204,110 - Pf6N2E2_4471 0.61 +1.8
Or see this region's nucleotide sequence