Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4415 and Pf6N2E2_4416 overlap by 4 nucleotides Pf6N2E2_4416 and Pf6N2E2_4417 are separated by 2 nucleotides Pf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotides Pf6N2E2_4418 and Pf6N2E2_4419 are separated by 4 nucleotides
Pf6N2E2_4415 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 5,148,899 to 5,149,603
_4415
Pf6N2E2_4416 - Tyrosine recombinase XerC, at 5,149,600 to 5,150,499
_4416
Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224
_4417
Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068
_4418
Pf6N2E2_4419 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 5,152,073 to 5,153,320
_4419
Position (kb)
5150
5151
5152 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.523 kb on + strand, within Pf6N2E2_4415 at 5149.685 kb on + strand at 5149.720 kb on + strand, within Pf6N2E2_4416 at 5149.721 kb on - strand, within Pf6N2E2_4416 at 5150.040 kb on + strand, within Pf6N2E2_4416 at 5150.078 kb on + strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.079 kb on - strand, within Pf6N2E2_4416 at 5150.253 kb on - strand, within Pf6N2E2_4416 at 5150.259 kb on - strand, within Pf6N2E2_4416 at 5150.509 kb on - strand at 5150.566 kb on + strand at 5150.566 kb on + strand at 5150.567 kb on - strand at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.599 kb on + strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.600 kb on - strand, within Pf6N2E2_4417 at 5150.820 kb on - strand, within Pf6N2E2_4417 at 5150.979 kb on + strand, within Pf6N2E2_4417 at 5150.980 kb on - strand, within Pf6N2E2_4417 at 5150.980 kb on - strand, within Pf6N2E2_4417 at 5151.261 kb on - strand at 5151.262 kb on + strand at 5151.263 kb on - strand at 5151.263 kb on - strand at 5151.907 kb on - strand, within Pf6N2E2_4418 at 5152.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,149,523 + Pf6N2E2_4415 0.89 +1.4 5,149,523 + Pf6N2E2_4415 0.89 +0.6 5,149,523 + Pf6N2E2_4415 0.89 -0.2 5,149,685 + -1.1 5,149,720 + Pf6N2E2_4416 0.13 -0.7 5,149,721 - Pf6N2E2_4416 0.13 -0.0 5,150,040 + Pf6N2E2_4416 0.49 -0.3 5,150,078 + Pf6N2E2_4416 0.53 -0.5 5,150,079 - Pf6N2E2_4416 0.53 -0.8 5,150,079 - Pf6N2E2_4416 0.53 -1.1 5,150,253 - Pf6N2E2_4416 0.73 -0.4 5,150,259 - Pf6N2E2_4416 0.73 -1.2 5,150,509 - -0.9 5,150,566 + -3.2 5,150,566 + -1.2 5,150,567 - -1.4 5,150,599 + Pf6N2E2_4417 0.13 -0.3 5,150,599 + Pf6N2E2_4417 0.13 -1.6 5,150,600 - Pf6N2E2_4417 0.14 -2.8 5,150,600 - Pf6N2E2_4417 0.14 -2.0 5,150,600 - Pf6N2E2_4417 0.14 -2.6 5,150,820 - Pf6N2E2_4417 0.44 -1.4 5,150,979 + Pf6N2E2_4417 0.66 -0.3 5,150,980 - Pf6N2E2_4417 0.66 -1.4 5,150,980 - Pf6N2E2_4417 0.66 -0.8 5,151,261 - +1.2 5,151,262 + -2.3 5,151,263 - -0.3 5,151,263 - +1.7 5,151,907 - Pf6N2E2_4418 0.81 +1.5 5,152,054 - +1.7
Or see this region's nucleotide sequence