Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4417

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4415 and Pf6N2E2_4416 overlap by 4 nucleotidesPf6N2E2_4416 and Pf6N2E2_4417 are separated by 2 nucleotidesPf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotidesPf6N2E2_4418 and Pf6N2E2_4419 are separated by 4 nucleotides Pf6N2E2_4415 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 5,148,899 to 5,149,603 _4415 Pf6N2E2_4416 - Tyrosine recombinase XerC, at 5,149,600 to 5,150,499 _4416 Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224 _4417 Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068 _4418 Pf6N2E2_4419 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 5,152,073 to 5,153,320 _4419 Position (kb) 5150 5151 5152Strain fitness (log2 ratio) -3 -2 -1 0 1at 5149.523 kb on + strand, within Pf6N2E2_4415at 5149.523 kb on + strand, within Pf6N2E2_4415at 5149.523 kb on + strand, within Pf6N2E2_4415at 5149.685 kb on + strandat 5149.720 kb on + strand, within Pf6N2E2_4416at 5149.721 kb on - strand, within Pf6N2E2_4416at 5150.040 kb on + strand, within Pf6N2E2_4416at 5150.078 kb on + strand, within Pf6N2E2_4416at 5150.079 kb on - strand, within Pf6N2E2_4416at 5150.079 kb on - strand, within Pf6N2E2_4416at 5150.253 kb on - strand, within Pf6N2E2_4416at 5150.259 kb on - strand, within Pf6N2E2_4416at 5150.509 kb on - strandat 5150.566 kb on + strandat 5150.566 kb on + strandat 5150.567 kb on - strandat 5150.599 kb on + strand, within Pf6N2E2_4417at 5150.599 kb on + strand, within Pf6N2E2_4417at 5150.600 kb on - strand, within Pf6N2E2_4417at 5150.600 kb on - strand, within Pf6N2E2_4417at 5150.600 kb on - strand, within Pf6N2E2_4417at 5150.820 kb on - strand, within Pf6N2E2_4417at 5150.979 kb on + strand, within Pf6N2E2_4417at 5150.980 kb on - strand, within Pf6N2E2_4417at 5150.980 kb on - strand, within Pf6N2E2_4417at 5151.261 kb on - strandat 5151.262 kb on + strandat 5151.263 kb on - strandat 5151.263 kb on - strandat 5151.907 kb on - strand, within Pf6N2E2_4418at 5152.054 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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5,149,523 + Pf6N2E2_4415 0.89 +1.4
5,149,523 + Pf6N2E2_4415 0.89 +0.6
5,149,523 + Pf6N2E2_4415 0.89 -0.2
5,149,685 + -1.1
5,149,720 + Pf6N2E2_4416 0.13 -0.7
5,149,721 - Pf6N2E2_4416 0.13 -0.0
5,150,040 + Pf6N2E2_4416 0.49 -0.3
5,150,078 + Pf6N2E2_4416 0.53 -0.5
5,150,079 - Pf6N2E2_4416 0.53 -0.8
5,150,079 - Pf6N2E2_4416 0.53 -1.1
5,150,253 - Pf6N2E2_4416 0.73 -0.4
5,150,259 - Pf6N2E2_4416 0.73 -1.2
5,150,509 - -0.9
5,150,566 + -3.2
5,150,566 + -1.2
5,150,567 - -1.4
5,150,599 + Pf6N2E2_4417 0.13 -0.3
5,150,599 + Pf6N2E2_4417 0.13 -1.6
5,150,600 - Pf6N2E2_4417 0.14 -2.8
5,150,600 - Pf6N2E2_4417 0.14 -2.0
5,150,600 - Pf6N2E2_4417 0.14 -2.6
5,150,820 - Pf6N2E2_4417 0.44 -1.4
5,150,979 + Pf6N2E2_4417 0.66 -0.3
5,150,980 - Pf6N2E2_4417 0.66 -1.4
5,150,980 - Pf6N2E2_4417 0.66 -0.8
5,151,261 - +1.2
5,151,262 + -2.3
5,151,263 - -0.3
5,151,263 - +1.7
5,151,907 - Pf6N2E2_4418 0.81 +1.5
5,152,054 - +1.7

Or see this region's nucleotide sequence