Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4370 and Pf6N2E2_4371 are separated by 44 nucleotides Pf6N2E2_4371 and Pf6N2E2_4372 are separated by 542 nucleotides Pf6N2E2_4372 and Pf6N2E2_4373 are separated by 53 nucleotides Pf6N2E2_4373 and Pf6N2E2_4374 are separated by 27 nucleotides
Pf6N2E2_4370 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 5,098,965 to 5,099,609
_4370
Pf6N2E2_4371 - hypothetical protein, at 5,099,654 to 5,099,968
_4371
Pf6N2E2_4372 - FIG00460773: hypothetical protein, at 5,100,511 to 5,101,755
_4372
Pf6N2E2_4373 - Acetylglutamate kinase (EC 2.7.2.8), at 5,101,809 to 5,102,714
_4373
Pf6N2E2_4374 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 5,102,742 to 5,104,139
_4374
Position (kb)
5100
5101
5102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5099.587 kb on - strand at 5099.641 kb on + strand at 5099.641 kb on + strand at 5099.681 kb on + strand at 5099.681 kb on + strand at 5099.681 kb on + strand at 5099.682 kb on - strand at 5099.769 kb on - strand, within Pf6N2E2_4371 at 5099.785 kb on - strand, within Pf6N2E2_4371 at 5099.896 kb on - strand, within Pf6N2E2_4371 at 5099.899 kb on + strand, within Pf6N2E2_4371 at 5099.899 kb on + strand, within Pf6N2E2_4371 at 5099.899 kb on + strand, within Pf6N2E2_4371 at 5099.900 kb on - strand, within Pf6N2E2_4371 at 5099.910 kb on + strand, within Pf6N2E2_4371 at 5099.910 kb on + strand, within Pf6N2E2_4371 at 5099.910 kb on + strand, within Pf6N2E2_4371 at 5099.910 kb on + strand, within Pf6N2E2_4371 at 5099.910 kb on + strand, within Pf6N2E2_4371 at 5099.911 kb on - strand, within Pf6N2E2_4371 at 5099.911 kb on - strand, within Pf6N2E2_4371 at 5099.911 kb on - strand, within Pf6N2E2_4371 at 5099.911 kb on - strand, within Pf6N2E2_4371 at 5099.911 kb on - strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.913 kb on + strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.914 kb on - strand, within Pf6N2E2_4371 at 5099.938 kb on + strand at 5099.938 kb on + strand at 5099.938 kb on + strand at 5099.938 kb on + strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.939 kb on - strand at 5099.951 kb on - strand at 5099.959 kb on - strand at 5099.987 kb on - strand at 5100.057 kb on + strand at 5100.058 kb on - strand at 5100.116 kb on - strand at 5100.124 kb on + strand at 5100.125 kb on - strand at 5100.176 kb on + strand at 5100.176 kb on + strand at 5100.177 kb on - strand at 5100.230 kb on + strand at 5100.230 kb on + strand at 5100.230 kb on + strand at 5100.231 kb on - strand at 5100.231 kb on - strand at 5100.231 kb on - strand at 5100.231 kb on - strand at 5100.231 kb on - strand at 5100.231 kb on - strand at 5100.249 kb on + strand at 5100.249 kb on + strand at 5100.250 kb on - strand at 5100.314 kb on + strand at 5100.314 kb on + strand at 5100.314 kb on + strand at 5100.337 kb on + strand at 5100.338 kb on - strand at 5100.338 kb on - strand at 5100.358 kb on + strand at 5100.358 kb on + strand at 5100.359 kb on - strand at 5100.467 kb on - strand at 5100.467 kb on - strand at 5100.473 kb on - strand at 5100.473 kb on - strand at 5100.480 kb on + strand at 5100.480 kb on + strand at 5100.593 kb on + strand at 5100.715 kb on + strand, within Pf6N2E2_4372 at 5100.755 kb on + strand, within Pf6N2E2_4372 at 5100.761 kb on + strand, within Pf6N2E2_4372 at 5100.782 kb on + strand, within Pf6N2E2_4372 at 5100.886 kb on + strand, within Pf6N2E2_4372 at 5100.896 kb on + strand, within Pf6N2E2_4372 at 5100.896 kb on + strand, within Pf6N2E2_4372 at 5100.896 kb on + strand, within Pf6N2E2_4372 at 5101.161 kb on + strand, within Pf6N2E2_4372 at 5101.161 kb on + strand, within Pf6N2E2_4372 at 5101.161 kb on + strand, within Pf6N2E2_4372 at 5101.161 kb on + strand, within Pf6N2E2_4372 at 5101.247 kb on + strand, within Pf6N2E2_4372 at 5101.295 kb on + strand, within Pf6N2E2_4372 at 5101.343 kb on + strand, within Pf6N2E2_4372 at 5101.343 kb on + strand, within Pf6N2E2_4372 at 5101.343 kb on + strand, within Pf6N2E2_4372 at 5101.398 kb on + strand, within Pf6N2E2_4372 at 5101.425 kb on + strand, within Pf6N2E2_4372 at 5101.466 kb on + strand, within Pf6N2E2_4372 at 5101.672 kb on + strand at 5101.672 kb on + strand at 5101.779 kb on + strand at 5101.780 kb on - strand at 5101.780 kb on - strand at 5101.780 kb on - strand at 5101.810 kb on + strand at 5101.811 kb on - strand at 5101.811 kb on - strand at 5101.811 kb on - strand at 5101.839 kb on - strand at 5101.839 kb on - strand at 5101.839 kb on - strand at 5102.752 kb on - strand at 5102.752 kb on - strand at 5102.752 kb on - strand at 5102.752 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,099,587 - -0.8 5,099,641 + +1.3 5,099,641 + -1.1 5,099,681 + -1.2 5,099,681 + -0.3 5,099,681 + -1.0 5,099,682 - +0.2 5,099,769 - Pf6N2E2_4371 0.37 +0.8 5,099,785 - Pf6N2E2_4371 0.42 +1.4 5,099,896 - Pf6N2E2_4371 0.77 -0.1 5,099,899 + Pf6N2E2_4371 0.78 -0.3 5,099,899 + Pf6N2E2_4371 0.78 +1.0 5,099,899 + Pf6N2E2_4371 0.78 +0.5 5,099,900 - Pf6N2E2_4371 0.78 +0.2 5,099,910 + Pf6N2E2_4371 0.81 -1.5 5,099,910 + Pf6N2E2_4371 0.81 +0.1 5,099,910 + Pf6N2E2_4371 0.81 -0.3 5,099,910 + Pf6N2E2_4371 0.81 -0.9 5,099,910 + Pf6N2E2_4371 0.81 +0.1 5,099,911 - Pf6N2E2_4371 0.82 -2.2 5,099,911 - Pf6N2E2_4371 0.82 +0.6 5,099,911 - Pf6N2E2_4371 0.82 +2.2 5,099,911 - Pf6N2E2_4371 0.82 +0.3 5,099,911 - Pf6N2E2_4371 0.82 +0.5 5,099,913 + Pf6N2E2_4371 0.82 +1.2 5,099,913 + Pf6N2E2_4371 0.82 -0.3 5,099,913 + Pf6N2E2_4371 0.82 -1.7 5,099,913 + Pf6N2E2_4371 0.82 -2.3 5,099,913 + Pf6N2E2_4371 0.82 -0.8 5,099,913 + Pf6N2E2_4371 0.82 -0.3 5,099,913 + Pf6N2E2_4371 0.82 +1.6 5,099,913 + Pf6N2E2_4371 0.82 +0.9 5,099,913 + Pf6N2E2_4371 0.82 +0.1 5,099,913 + Pf6N2E2_4371 0.82 -2.2 5,099,913 + Pf6N2E2_4371 0.82 +0.0 5,099,913 + Pf6N2E2_4371 0.82 +0.4 5,099,914 - Pf6N2E2_4371 0.83 +0.5 5,099,914 - Pf6N2E2_4371 0.83 -0.9 5,099,914 - Pf6N2E2_4371 0.83 -0.5 5,099,914 - Pf6N2E2_4371 0.83 -0.8 5,099,914 - Pf6N2E2_4371 0.83 +1.9 5,099,914 - Pf6N2E2_4371 0.83 -1.4 5,099,914 - Pf6N2E2_4371 0.83 -0.9 5,099,914 - Pf6N2E2_4371 0.83 -0.5 5,099,914 - Pf6N2E2_4371 0.83 +2.1 5,099,914 - Pf6N2E2_4371 0.83 +1.1 5,099,938 + -2.0 5,099,938 + -0.3 5,099,938 + +0.5 5,099,938 + +0.6 5,099,939 - +1.6 5,099,939 - -2.8 5,099,939 - +0.2 5,099,939 - -1.1 5,099,939 - -0.3 5,099,939 - +1.0 5,099,939 - +0.3 5,099,939 - -1.7 5,099,939 - -1.3 5,099,939 - +0.7 5,099,951 - -0.0 5,099,959 - +1.7 5,099,987 - +1.8 5,100,057 + +1.5 5,100,058 - +0.5 5,100,116 - -0.8 5,100,124 + +0.4 5,100,125 - +2.0 5,100,176 + -0.1 5,100,176 + +1.1 5,100,177 - -1.2 5,100,230 + -0.9 5,100,230 + +2.3 5,100,230 + +1.6 5,100,231 - +1.8 5,100,231 - +0.3 5,100,231 - -0.6 5,100,231 - -0.7 5,100,231 - +0.5 5,100,231 - -1.1 5,100,249 + -0.1 5,100,249 + +0.9 5,100,250 - +1.3 5,100,314 + -0.7 5,100,314 + +0.6 5,100,314 + +0.3 5,100,337 + +0.2 5,100,338 - +0.4 5,100,338 - -0.3 5,100,358 + -2.4 5,100,358 + -0.2 5,100,359 - +0.2 5,100,467 - -1.1 5,100,467 - +1.3 5,100,473 - -1.1 5,100,473 - +1.1 5,100,480 + +0.6 5,100,480 + -0.9 5,100,593 + -0.9 5,100,715 + Pf6N2E2_4372 0.16 -0.5 5,100,755 + Pf6N2E2_4372 0.20 +2.6 5,100,761 + Pf6N2E2_4372 0.20 -1.4 5,100,782 + Pf6N2E2_4372 0.22 -2.0 5,100,886 + Pf6N2E2_4372 0.30 -0.2 5,100,896 + Pf6N2E2_4372 0.31 -2.0 5,100,896 + Pf6N2E2_4372 0.31 -2.1 5,100,896 + Pf6N2E2_4372 0.31 -2.0 5,101,161 + Pf6N2E2_4372 0.52 -0.6 5,101,161 + Pf6N2E2_4372 0.52 -1.9 5,101,161 + Pf6N2E2_4372 0.52 -1.8 5,101,161 + Pf6N2E2_4372 0.52 -1.2 5,101,247 + Pf6N2E2_4372 0.59 -0.2 5,101,295 + Pf6N2E2_4372 0.63 +0.9 5,101,343 + Pf6N2E2_4372 0.67 -1.0 5,101,343 + Pf6N2E2_4372 0.67 -1.8 5,101,343 + Pf6N2E2_4372 0.67 +0.9 5,101,398 + Pf6N2E2_4372 0.71 +0.2 5,101,425 + Pf6N2E2_4372 0.73 -0.3 5,101,466 + Pf6N2E2_4372 0.77 -0.9 5,101,672 + -0.2 5,101,672 + -1.2 5,101,779 + +0.5 5,101,780 - -1.3 5,101,780 - +1.1 5,101,780 - -0.2 5,101,810 + -1.0 5,101,811 - +1.4 5,101,811 - -0.3 5,101,811 - -1.3 5,101,839 - +0.0 5,101,839 - +0.3 5,101,839 - +0.7 5,102,752 - +0.0 5,102,752 - +1.1 5,102,752 - -1.0 5,102,752 - -0.7
Or see this region's nucleotide sequence