Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4351 and Pf6N2E2_4352 are separated by 148 nucleotides Pf6N2E2_4352 and Pf6N2E2_4353 are separated by 9 nucleotides
Pf6N2E2_4351 - Predicted signal transduction protein, at 5,080,537 to 5,081,868
_4351
Pf6N2E2_4352 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 5,082,017 to 5,084,092
_4352
Pf6N2E2_4353 - Hydrogen peroxide-inducible genes activator, at 5,084,102 to 5,085,022
_4353
Position (kb)
5082
5083
5084
5085 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.072 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.219 kb on + strand, within Pf6N2E2_4351 at 5081.606 kb on + strand, within Pf6N2E2_4351 at 5081.607 kb on - strand, within Pf6N2E2_4351 at 5081.809 kb on + strand at 5081.809 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.972 kb on + strand at 5081.973 kb on - strand at 5082.777 kb on + strand, within Pf6N2E2_4352 at 5083.701 kb on + strand, within Pf6N2E2_4352 at 5083.713 kb on + strand, within Pf6N2E2_4352 at 5083.713 kb on + strand, within Pf6N2E2_4352 at 5083.713 kb on + strand, within Pf6N2E2_4352 at 5083.713 kb on + strand, within Pf6N2E2_4352 at 5083.714 kb on - strand, within Pf6N2E2_4352 at 5083.714 kb on - strand, within Pf6N2E2_4352 at 5083.809 kb on + strand, within Pf6N2E2_4352 at 5083.931 kb on + strand at 5083.932 kb on - strand at 5084.103 kb on + strand at 5084.103 kb on + strand at 5084.104 kb on - strand at 5084.104 kb on - strand at 5084.104 kb on - strand at 5084.263 kb on - strand, within Pf6N2E2_4353 at 5084.303 kb on - strand, within Pf6N2E2_4353 at 5084.527 kb on - strand, within Pf6N2E2_4353 at 5084.527 kb on - strand, within Pf6N2E2_4353 at 5084.527 kb on - strand, within Pf6N2E2_4353 at 5084.527 kb on - strand, within Pf6N2E2_4353 at 5084.542 kb on - strand, within Pf6N2E2_4353 at 5084.655 kb on + strand, within Pf6N2E2_4353 at 5085.001 kb on - strand at 5085.001 kb on - strand at 5085.006 kb on - strand at 5085.006 kb on - strand at 5085.054 kb on + strand at 5085.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,081,054 + Pf6N2E2_4351 0.39 +4.8 5,081,054 + Pf6N2E2_4351 0.39 +4.8 5,081,055 - Pf6N2E2_4351 0.39 +6.2 5,081,055 - Pf6N2E2_4351 0.39 +5.6 5,081,055 - Pf6N2E2_4351 0.39 +5.8 5,081,055 - Pf6N2E2_4351 0.39 +5.5 5,081,055 - Pf6N2E2_4351 0.39 +4.3 5,081,055 - Pf6N2E2_4351 0.39 +2.9 5,081,055 - Pf6N2E2_4351 0.39 +5.6 5,081,055 - Pf6N2E2_4351 0.39 +5.4 5,081,072 + Pf6N2E2_4351 0.40 +5.2 5,081,216 + Pf6N2E2_4351 0.51 +4.4 5,081,216 + Pf6N2E2_4351 0.51 +5.8 5,081,219 + Pf6N2E2_4351 0.51 +4.4 5,081,606 + Pf6N2E2_4351 0.80 +5.8 5,081,607 - Pf6N2E2_4351 0.80 +6.1 5,081,809 + +5.7 5,081,809 + +5.2 5,081,970 + +4.7 5,081,970 + +1.2 5,081,970 + +5.3 5,081,970 + +3.5 5,081,970 + +0.6 5,081,970 + +4.7 5,081,971 - -1.6 5,081,971 - -1.3 5,081,971 - -2.4 5,081,971 - -0.8 5,081,972 + +0.4 5,081,973 - -1.3 5,082,777 + Pf6N2E2_4352 0.37 -0.5 5,083,701 + Pf6N2E2_4352 0.81 -1.5 5,083,713 + Pf6N2E2_4352 0.82 -1.5 5,083,713 + Pf6N2E2_4352 0.82 -1.2 5,083,713 + Pf6N2E2_4352 0.82 -0.3 5,083,713 + Pf6N2E2_4352 0.82 -0.4 5,083,714 - Pf6N2E2_4352 0.82 -1.7 5,083,714 - Pf6N2E2_4352 0.82 -1.1 5,083,809 + Pf6N2E2_4352 0.86 -0.7 5,083,931 + -1.1 5,083,932 - -1.1 5,084,103 + -2.0 5,084,103 + -1.6 5,084,104 - +1.4 5,084,104 - +0.6 5,084,104 - +0.9 5,084,263 - Pf6N2E2_4353 0.17 -1.7 5,084,303 - Pf6N2E2_4353 0.22 -1.8 5,084,527 - Pf6N2E2_4353 0.46 -3.9 5,084,527 - Pf6N2E2_4353 0.46 -1.6 5,084,527 - Pf6N2E2_4353 0.46 -0.7 5,084,527 - Pf6N2E2_4353 0.46 +0.2 5,084,542 - Pf6N2E2_4353 0.48 -1.2 5,084,655 + Pf6N2E2_4353 0.60 -2.0 5,085,001 - -1.1 5,085,001 - -0.1 5,085,006 - -3.3 5,085,006 - -1.9 5,085,054 + -3.0 5,085,055 - -0.1
Or see this region's nucleotide sequence