Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4325 and Pf6N2E2_4326 are separated by 504 nucleotides Pf6N2E2_4326 and Pf6N2E2_4327 are separated by 158 nucleotides
Pf6N2E2_4325 - hypothetical protein, at 5,045,283 to 5,045,816
_4325
Pf6N2E2_4326 - VgrG protein, at 5,046,321 to 5,048,261
_4326
Pf6N2E2_4327 - ClpB protein, at 5,048,420 to 5,051,110
_4327
Position (kb)
5046
5047
5048
5049 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5045.807 kb on + strand at 5045.841 kb on + strand at 5045.841 kb on + strand at 5045.841 kb on + strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.842 kb on - strand at 5045.889 kb on - strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.945 kb on + strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.946 kb on - strand at 5045.949 kb on + strand at 5045.949 kb on + strand at 5045.949 kb on + strand at 5045.950 kb on - strand at 5045.950 kb on - strand at 5045.989 kb on + strand at 5045.989 kb on + strand at 5045.989 kb on + strand at 5045.990 kb on - strand at 5046.096 kb on + strand at 5046.096 kb on + strand at 5046.097 kb on - strand at 5046.138 kb on - strand at 5046.469 kb on + strand at 5046.470 kb on - strand at 5046.470 kb on - strand at 5046.470 kb on - strand at 5046.473 kb on + strand at 5046.473 kb on + strand at 5046.473 kb on + strand at 5046.474 kb on - strand at 5046.495 kb on - strand at 5046.497 kb on + strand at 5046.497 kb on + strand at 5046.498 kb on - strand at 5046.574 kb on + strand, within Pf6N2E2_4326 at 5046.575 kb on - strand, within Pf6N2E2_4326 at 5046.575 kb on - strand, within Pf6N2E2_4326 at 5046.881 kb on - strand, within Pf6N2E2_4326 at 5046.904 kb on + strand, within Pf6N2E2_4326 at 5046.904 kb on + strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.286 kb on - strand, within Pf6N2E2_4326 at 5047.310 kb on - strand, within Pf6N2E2_4326 at 5047.310 kb on - strand, within Pf6N2E2_4326 at 5047.441 kb on + strand, within Pf6N2E2_4326 at 5047.442 kb on - strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.468 kb on + strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.469 kb on - strand, within Pf6N2E2_4326 at 5047.568 kb on - strand, within Pf6N2E2_4326 at 5047.625 kb on + strand, within Pf6N2E2_4326 at 5047.625 kb on + strand, within Pf6N2E2_4326 at 5047.626 kb on - strand, within Pf6N2E2_4326 at 5047.626 kb on - strand, within Pf6N2E2_4326 at 5047.661 kb on - strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.663 kb on + strand, within Pf6N2E2_4326 at 5047.757 kb on - strand, within Pf6N2E2_4326 at 5047.757 kb on - strand, within Pf6N2E2_4326 at 5047.760 kb on - strand, within Pf6N2E2_4326 at 5047.760 kb on - strand, within Pf6N2E2_4326 at 5047.813 kb on + strand, within Pf6N2E2_4326 at 5047.825 kb on + strand, within Pf6N2E2_4326 at 5047.825 kb on + strand, within Pf6N2E2_4326 at 5047.840 kb on + strand, within Pf6N2E2_4326 at 5048.149 kb on + strand at 5048.256 kb on + strand at 5048.279 kb on + strand at 5048.336 kb on + strand at 5048.336 kb on + strand at 5048.442 kb on + strand at 5048.443 kb on - strand at 5048.724 kb on + strand, within Pf6N2E2_4327 at 5048.725 kb on - strand, within Pf6N2E2_4327 at 5049.012 kb on + strand, within Pf6N2E2_4327 at 5049.013 kb on - strand, within Pf6N2E2_4327 at 5049.066 kb on + strand, within Pf6N2E2_4327 at 5049.066 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.156 kb on + strand, within Pf6N2E2_4327 at 5049.157 kb on - strand, within Pf6N2E2_4327 at 5049.157 kb on - strand, within Pf6N2E2_4327
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,045,807 + +0.7 5,045,841 + -0.2 5,045,841 + +0.7 5,045,841 + -0.3 5,045,842 - +0.1 5,045,842 - -2.1 5,045,842 - +0.4 5,045,842 - -0.3 5,045,889 - +1.7 5,045,945 + -1.7 5,045,945 + +0.0 5,045,945 + +1.7 5,045,945 + -2.0 5,045,945 + +1.1 5,045,946 - +1.2 5,045,946 - +1.5 5,045,946 - +0.6 5,045,946 - +1.6 5,045,946 - -0.1 5,045,946 - -0.0 5,045,946 - -2.5 5,045,949 + +0.7 5,045,949 + -2.7 5,045,949 + -0.4 5,045,950 - +1.2 5,045,950 - -1.2 5,045,989 + -1.0 5,045,989 + +0.2 5,045,989 + -0.2 5,045,990 - -0.5 5,046,096 + -0.3 5,046,096 + -1.8 5,046,097 - -0.6 5,046,138 - +0.6 5,046,469 + +0.7 5,046,470 - +1.3 5,046,470 - +0.2 5,046,470 - +0.7 5,046,473 + -0.9 5,046,473 + -1.6 5,046,473 + -0.3 5,046,474 - +1.0 5,046,495 - +0.0 5,046,497 + +2.2 5,046,497 + -2.0 5,046,498 - +0.4 5,046,574 + Pf6N2E2_4326 0.13 +0.1 5,046,575 - Pf6N2E2_4326 0.13 +0.7 5,046,575 - Pf6N2E2_4326 0.13 +0.8 5,046,881 - Pf6N2E2_4326 0.29 -0.2 5,046,904 + Pf6N2E2_4326 0.30 +0.0 5,046,904 + Pf6N2E2_4326 0.30 +2.0 5,047,286 - Pf6N2E2_4326 0.50 +0.2 5,047,286 - Pf6N2E2_4326 0.50 +0.2 5,047,286 - Pf6N2E2_4326 0.50 +1.0 5,047,286 - Pf6N2E2_4326 0.50 +0.9 5,047,310 - Pf6N2E2_4326 0.51 +1.2 5,047,310 - Pf6N2E2_4326 0.51 +1.5 5,047,441 + Pf6N2E2_4326 0.58 +1.2 5,047,442 - Pf6N2E2_4326 0.58 -0.5 5,047,468 + Pf6N2E2_4326 0.59 -1.0 5,047,468 + Pf6N2E2_4326 0.59 +0.8 5,047,468 + Pf6N2E2_4326 0.59 -0.2 5,047,468 + Pf6N2E2_4326 0.59 +0.8 5,047,468 + Pf6N2E2_4326 0.59 +0.1 5,047,468 + Pf6N2E2_4326 0.59 -0.7 5,047,468 + Pf6N2E2_4326 0.59 +0.5 5,047,468 + Pf6N2E2_4326 0.59 -0.7 5,047,469 - Pf6N2E2_4326 0.59 +0.7 5,047,469 - Pf6N2E2_4326 0.59 +0.4 5,047,469 - Pf6N2E2_4326 0.59 +1.1 5,047,469 - Pf6N2E2_4326 0.59 +1.1 5,047,469 - Pf6N2E2_4326 0.59 -0.6 5,047,469 - Pf6N2E2_4326 0.59 +0.8 5,047,469 - Pf6N2E2_4326 0.59 +1.0 5,047,469 - Pf6N2E2_4326 0.59 +0.0 5,047,469 - Pf6N2E2_4326 0.59 -0.6 5,047,568 - Pf6N2E2_4326 0.64 +0.4 5,047,625 + Pf6N2E2_4326 0.67 +0.9 5,047,625 + Pf6N2E2_4326 0.67 -0.8 5,047,626 - Pf6N2E2_4326 0.67 +2.1 5,047,626 - Pf6N2E2_4326 0.67 -0.7 5,047,661 - Pf6N2E2_4326 0.69 +2.1 5,047,663 + Pf6N2E2_4326 0.69 +1.4 5,047,663 + Pf6N2E2_4326 0.69 +1.1 5,047,663 + Pf6N2E2_4326 0.69 +0.8 5,047,757 - Pf6N2E2_4326 0.74 +0.7 5,047,757 - Pf6N2E2_4326 0.74 +1.2 5,047,760 - Pf6N2E2_4326 0.74 +1.0 5,047,760 - Pf6N2E2_4326 0.74 -0.8 5,047,813 + Pf6N2E2_4326 0.77 -1.7 5,047,825 + Pf6N2E2_4326 0.77 +0.5 5,047,825 + Pf6N2E2_4326 0.77 -0.6 5,047,840 + Pf6N2E2_4326 0.78 +0.4 5,048,149 + -1.8 5,048,256 + +0.5 5,048,279 + +1.0 5,048,336 + -0.1 5,048,336 + -0.1 5,048,442 + -1.7 5,048,443 - -0.4 5,048,724 + Pf6N2E2_4327 0.11 -0.2 5,048,725 - Pf6N2E2_4327 0.11 -1.1 5,049,012 + Pf6N2E2_4327 0.22 +1.9 5,049,013 - Pf6N2E2_4327 0.22 -0.8 5,049,066 + Pf6N2E2_4327 0.24 -0.2 5,049,066 + Pf6N2E2_4327 0.24 +0.3 5,049,156 + Pf6N2E2_4327 0.27 -2.0 5,049,156 + Pf6N2E2_4327 0.27 -1.1 5,049,156 + Pf6N2E2_4327 0.27 -0.3 5,049,157 - Pf6N2E2_4327 0.27 -0.5 5,049,157 - Pf6N2E2_4327 0.27 +1.0
Or see this region's nucleotide sequence