Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4263 and Pf6N2E2_4264 overlap by 1 nucleotides Pf6N2E2_4264 and Pf6N2E2_4265 overlap by 4 nucleotides Pf6N2E2_4265 and Pf6N2E2_4266 are separated by 27 nucleotides
Pf6N2E2_4263 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 4,961,392 to 4,962,600
_4263
Pf6N2E2_4264 - FIG00954044: hypothetical protein, at 4,962,600 to 4,963,250
_4264
Pf6N2E2_4265 - NADH:ubiquinone oxidoreductase 49 kD subunit 7, at 4,963,247 to 4,963,600
_4265
Pf6N2E2_4266 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 4,963,628 to 4,964,593
_4266
Position (kb)
4963
4964 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4962.665 kb on - strand, within Pf6N2E2_4264 at 4962.861 kb on + strand, within Pf6N2E2_4264 at 4962.861 kb on + strand, within Pf6N2E2_4264 at 4962.862 kb on - strand, within Pf6N2E2_4264 at 4962.862 kb on - strand, within Pf6N2E2_4264 at 4962.913 kb on + strand, within Pf6N2E2_4264 at 4962.991 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.014 kb on + strand, within Pf6N2E2_4264 at 4963.015 kb on - strand, within Pf6N2E2_4264 at 4963.015 kb on - strand, within Pf6N2E2_4264 at 4963.015 kb on - strand, within Pf6N2E2_4264 at 4963.015 kb on - strand, within Pf6N2E2_4264 at 4963.142 kb on + strand, within Pf6N2E2_4264 at 4963.143 kb on - strand, within Pf6N2E2_4264 at 4963.228 kb on + strand at 4963.228 kb on + strand at 4963.228 kb on + strand at 4963.229 kb on - strand at 4963.229 kb on - strand at 4963.229 kb on - strand at 4963.248 kb on + strand at 4963.299 kb on + strand, within Pf6N2E2_4265 at 4963.326 kb on + strand, within Pf6N2E2_4265 at 4963.326 kb on + strand, within Pf6N2E2_4265 at 4963.326 kb on + strand, within Pf6N2E2_4265 at 4963.326 kb on + strand, within Pf6N2E2_4265 at 4963.327 kb on - strand, within Pf6N2E2_4265 at 4963.327 kb on - strand, within Pf6N2E2_4265 at 4963.351 kb on + strand, within Pf6N2E2_4265 at 4963.351 kb on + strand, within Pf6N2E2_4265 at 4963.351 kb on + strand, within Pf6N2E2_4265 at 4963.351 kb on + strand, within Pf6N2E2_4265 at 4963.352 kb on - strand, within Pf6N2E2_4265 at 4963.410 kb on + strand, within Pf6N2E2_4265 at 4963.410 kb on + strand, within Pf6N2E2_4265 at 4963.410 kb on + strand, within Pf6N2E2_4265 at 4963.410 kb on + strand, within Pf6N2E2_4265 at 4963.410 kb on + strand, within Pf6N2E2_4265 at 4963.411 kb on - strand, within Pf6N2E2_4265 at 4963.411 kb on - strand, within Pf6N2E2_4265 at 4963.411 kb on - strand, within Pf6N2E2_4265 at 4963.411 kb on - strand, within Pf6N2E2_4265 at 4963.411 kb on - strand, within Pf6N2E2_4265 at 4963.600 kb on + strand at 4963.600 kb on + strand at 4963.600 kb on + strand at 4963.601 kb on - strand at 4963.627 kb on + strand at 4963.627 kb on + strand at 4963.627 kb on + strand at 4963.627 kb on + strand at 4963.627 kb on + strand at 4963.627 kb on + strand at 4963.628 kb on - strand at 4963.628 kb on - strand at 4963.628 kb on - strand at 4963.801 kb on + strand, within Pf6N2E2_4266 at 4963.881 kb on + strand, within Pf6N2E2_4266 at 4963.882 kb on - strand, within Pf6N2E2_4266 at 4963.882 kb on - strand, within Pf6N2E2_4266 at 4963.882 kb on - strand, within Pf6N2E2_4266 at 4963.885 kb on + strand, within Pf6N2E2_4266 at 4963.886 kb on - strand, within Pf6N2E2_4266 at 4964.162 kb on + strand, within Pf6N2E2_4266 at 4964.163 kb on - strand, within Pf6N2E2_4266 at 4964.163 kb on - strand, within Pf6N2E2_4266 at 4964.163 kb on - strand, within Pf6N2E2_4266 at 4964.163 kb on - strand, within Pf6N2E2_4266 at 4964.163 kb on - strand, within Pf6N2E2_4266 at 4964.237 kb on - strand, within Pf6N2E2_4266 at 4964.247 kb on + strand, within Pf6N2E2_4266 at 4964.247 kb on + strand, within Pf6N2E2_4266 at 4964.248 kb on - strand, within Pf6N2E2_4266 at 4964.248 kb on - strand, within Pf6N2E2_4266 at 4964.309 kb on + strand, within Pf6N2E2_4266 at 4964.309 kb on + strand, within Pf6N2E2_4266 at 4964.309 kb on + strand, within Pf6N2E2_4266 at 4964.309 kb on + strand, within Pf6N2E2_4266 at 4964.309 kb on + strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.310 kb on - strand, within Pf6N2E2_4266 at 4964.335 kb on + strand, within Pf6N2E2_4266 at 4964.336 kb on - strand, within Pf6N2E2_4266 at 4964.395 kb on - strand, within Pf6N2E2_4266
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,962,665 - Pf6N2E2_4264 0.10 -0.6 4,962,861 + Pf6N2E2_4264 0.40 -0.6 4,962,861 + Pf6N2E2_4264 0.40 -0.3 4,962,862 - Pf6N2E2_4264 0.40 +0.2 4,962,862 - Pf6N2E2_4264 0.40 +1.8 4,962,913 + Pf6N2E2_4264 0.48 -0.1 4,962,991 + Pf6N2E2_4264 0.60 -1.0 4,963,014 + Pf6N2E2_4264 0.64 +1.2 4,963,014 + Pf6N2E2_4264 0.64 -0.8 4,963,014 + Pf6N2E2_4264 0.64 -2.0 4,963,014 + Pf6N2E2_4264 0.64 +0.2 4,963,014 + Pf6N2E2_4264 0.64 +0.1 4,963,014 + Pf6N2E2_4264 0.64 -0.6 4,963,015 - Pf6N2E2_4264 0.64 -0.3 4,963,015 - Pf6N2E2_4264 0.64 +0.0 4,963,015 - Pf6N2E2_4264 0.64 -2.1 4,963,015 - Pf6N2E2_4264 0.64 +0.2 4,963,142 + Pf6N2E2_4264 0.83 -0.7 4,963,143 - Pf6N2E2_4264 0.83 -0.1 4,963,228 + -0.5 4,963,228 + -1.3 4,963,228 + +0.2 4,963,229 - -1.4 4,963,229 - -0.7 4,963,229 - +0.4 4,963,248 + -0.6 4,963,299 + Pf6N2E2_4265 0.15 -0.3 4,963,326 + Pf6N2E2_4265 0.22 -0.8 4,963,326 + Pf6N2E2_4265 0.22 -0.8 4,963,326 + Pf6N2E2_4265 0.22 -2.2 4,963,326 + Pf6N2E2_4265 0.22 -0.8 4,963,327 - Pf6N2E2_4265 0.23 +0.2 4,963,327 - Pf6N2E2_4265 0.23 +0.1 4,963,351 + Pf6N2E2_4265 0.29 +0.2 4,963,351 + Pf6N2E2_4265 0.29 +3.2 4,963,351 + Pf6N2E2_4265 0.29 -0.3 4,963,351 + Pf6N2E2_4265 0.29 -1.0 4,963,352 - Pf6N2E2_4265 0.30 -0.6 4,963,410 + Pf6N2E2_4265 0.46 -0.3 4,963,410 + Pf6N2E2_4265 0.46 -0.7 4,963,410 + Pf6N2E2_4265 0.46 -1.0 4,963,410 + Pf6N2E2_4265 0.46 -0.9 4,963,410 + Pf6N2E2_4265 0.46 -0.6 4,963,411 - Pf6N2E2_4265 0.46 +1.1 4,963,411 - Pf6N2E2_4265 0.46 +1.3 4,963,411 - Pf6N2E2_4265 0.46 -0.7 4,963,411 - Pf6N2E2_4265 0.46 -0.1 4,963,411 - Pf6N2E2_4265 0.46 -0.3 4,963,600 + -1.2 4,963,600 + -1.1 4,963,600 + +0.2 4,963,601 - -1.2 4,963,627 + +1.6 4,963,627 + +1.0 4,963,627 + -1.6 4,963,627 + -1.7 4,963,627 + +0.6 4,963,627 + +1.5 4,963,628 - -2.4 4,963,628 - +0.4 4,963,628 - -0.7 4,963,801 + Pf6N2E2_4266 0.18 -1.1 4,963,881 + Pf6N2E2_4266 0.26 -0.4 4,963,882 - Pf6N2E2_4266 0.26 +0.7 4,963,882 - Pf6N2E2_4266 0.26 +0.8 4,963,882 - Pf6N2E2_4266 0.26 +0.6 4,963,885 + Pf6N2E2_4266 0.27 +0.4 4,963,886 - Pf6N2E2_4266 0.27 -0.1 4,964,162 + Pf6N2E2_4266 0.55 +0.6 4,964,163 - Pf6N2E2_4266 0.55 +0.5 4,964,163 - Pf6N2E2_4266 0.55 -1.2 4,964,163 - Pf6N2E2_4266 0.55 -1.2 4,964,163 - Pf6N2E2_4266 0.55 +0.4 4,964,163 - Pf6N2E2_4266 0.55 +0.3 4,964,237 - Pf6N2E2_4266 0.63 -0.9 4,964,247 + Pf6N2E2_4266 0.64 -0.2 4,964,247 + Pf6N2E2_4266 0.64 -0.8 4,964,248 - Pf6N2E2_4266 0.64 +0.2 4,964,248 - Pf6N2E2_4266 0.64 +0.2 4,964,309 + Pf6N2E2_4266 0.70 -0.2 4,964,309 + Pf6N2E2_4266 0.70 +0.8 4,964,309 + Pf6N2E2_4266 0.70 -1.5 4,964,309 + Pf6N2E2_4266 0.70 +0.4 4,964,309 + Pf6N2E2_4266 0.70 +0.5 4,964,310 - Pf6N2E2_4266 0.71 +0.2 4,964,310 - Pf6N2E2_4266 0.71 -0.7 4,964,310 - Pf6N2E2_4266 0.71 -0.1 4,964,310 - Pf6N2E2_4266 0.71 -1.1 4,964,310 - Pf6N2E2_4266 0.71 -0.7 4,964,310 - Pf6N2E2_4266 0.71 -1.3 4,964,335 + Pf6N2E2_4266 0.73 -0.4 4,964,336 - Pf6N2E2_4266 0.73 +0.6 4,964,395 - Pf6N2E2_4266 0.79 -0.1
Or see this region's nucleotide sequence