Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_417 and Pf6N2E2_418 are separated by 33 nucleotides Pf6N2E2_418 and Pf6N2E2_419 are separated by 39 nucleotides Pf6N2E2_419 and Pf6N2E2_420 are separated by 15 nucleotides Pf6N2E2_420 and Pf6N2E2_421 are separated by 11 nucleotides
Pf6N2E2_417 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 484,348 to 486,006
_417
Pf6N2E2_418 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 486,040 to 488,142
_418
Pf6N2E2_419 - STAS domain protein, at 488,182 to 488,466
_419
Pf6N2E2_420 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 488,482 to 488,850
_420
Pf6N2E2_421 - Methyl-accepting chemotaxis protein, at 488,862 to 490,013
_421
Position (kb)
486
487
488
489 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 485.630 kb on + strand, within Pf6N2E2_417 at 485.631 kb on - strand, within Pf6N2E2_417 at 485.700 kb on - strand, within Pf6N2E2_417 at 485.810 kb on + strand, within Pf6N2E2_417 at 485.810 kb on + strand, within Pf6N2E2_417 at 485.811 kb on - strand, within Pf6N2E2_417 at 485.985 kb on + strand at 485.985 kb on + strand at 485.986 kb on - strand at 485.986 kb on - strand at 485.986 kb on - strand at 485.993 kb on + strand at 485.993 kb on + strand at 485.993 kb on + strand at 485.993 kb on + strand at 485.993 kb on + strand at 485.994 kb on - strand at 485.994 kb on - strand at 485.994 kb on - strand at 486.014 kb on + strand at 486.014 kb on + strand at 486.014 kb on + strand at 486.014 kb on + strand at 486.015 kb on - strand at 486.034 kb on + strand at 486.061 kb on + strand at 486.062 kb on - strand at 486.371 kb on + strand, within Pf6N2E2_418 at 486.371 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.449 kb on + strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.450 kb on - strand, within Pf6N2E2_418 at 486.668 kb on + strand, within Pf6N2E2_418 at 486.668 kb on + strand, within Pf6N2E2_418 at 486.668 kb on + strand, within Pf6N2E2_418 at 486.669 kb on - strand, within Pf6N2E2_418 at 486.669 kb on - strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.226 kb on + strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.227 kb on - strand, within Pf6N2E2_418 at 487.520 kb on + strand, within Pf6N2E2_418 at 487.520 kb on + strand, within Pf6N2E2_418 at 487.521 kb on - strand, within Pf6N2E2_418 at 487.521 kb on - strand, within Pf6N2E2_418 at 487.521 kb on - strand, within Pf6N2E2_418 at 487.608 kb on - strand, within Pf6N2E2_418 at 487.754 kb on + strand, within Pf6N2E2_418 at 487.754 kb on + strand, within Pf6N2E2_418 at 488.148 kb on + strand at 488.149 kb on - strand at 488.170 kb on - strand at 488.170 kb on - strand at 488.170 kb on - strand at 488.278 kb on + strand, within Pf6N2E2_419 at 488.278 kb on + strand, within Pf6N2E2_419 at 488.278 kb on + strand, within Pf6N2E2_419 at 488.278 kb on + strand, within Pf6N2E2_419 at 488.279 kb on - strand, within Pf6N2E2_419 at 488.279 kb on - strand, within Pf6N2E2_419 at 488.279 kb on - strand, within Pf6N2E2_419 at 488.279 kb on - strand, within Pf6N2E2_419 at 488.400 kb on - strand, within Pf6N2E2_419 at 488.400 kb on - strand, within Pf6N2E2_419 at 488.454 kb on - strand at 488.631 kb on - strand, within Pf6N2E2_420 at 488.631 kb on - strand, within Pf6N2E2_420 at 488.836 kb on + strand at 488.837 kb on - strand at 488.864 kb on - strand at 488.864 kb on - strand at 488.941 kb on + strand at 488.941 kb on + strand at 488.941 kb on + strand at 488.941 kb on + strand at 488.941 kb on + strand at 488.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 485,630 + Pf6N2E2_417 0.77 -1.7 485,631 - Pf6N2E2_417 0.77 -0.9 485,700 - Pf6N2E2_417 0.81 +0.4 485,810 + Pf6N2E2_417 0.88 -0.6 485,810 + Pf6N2E2_417 0.88 +1.1 485,811 - Pf6N2E2_417 0.88 -0.6 485,985 + -1.2 485,985 + -0.5 485,986 - +1.1 485,986 - -0.5 485,986 - +0.2 485,993 + -0.6 485,993 + +0.8 485,993 + +2.1 485,993 + -1.0 485,993 + -2.5 485,994 - -0.2 485,994 - -1.3 485,994 - -0.2 486,014 + -1.1 486,014 + +0.1 486,014 + +0.3 486,014 + +0.5 486,015 - +0.2 486,034 + +0.9 486,061 + +0.6 486,062 - -1.8 486,371 + Pf6N2E2_418 0.16 +0.1 486,371 + Pf6N2E2_418 0.16 -1.8 486,449 + Pf6N2E2_418 0.19 -1.0 486,449 + Pf6N2E2_418 0.19 +0.0 486,449 + Pf6N2E2_418 0.19 +0.3 486,449 + Pf6N2E2_418 0.19 -0.6 486,449 + Pf6N2E2_418 0.19 -1.0 486,449 + Pf6N2E2_418 0.19 -0.2 486,450 - Pf6N2E2_418 0.19 +0.6 486,450 - Pf6N2E2_418 0.19 -0.7 486,450 - Pf6N2E2_418 0.19 -0.5 486,450 - Pf6N2E2_418 0.19 +0.6 486,450 - Pf6N2E2_418 0.19 -1.0 486,450 - Pf6N2E2_418 0.19 +0.4 486,450 - Pf6N2E2_418 0.19 -1.3 486,668 + Pf6N2E2_418 0.30 +1.6 486,668 + Pf6N2E2_418 0.30 -1.5 486,668 + Pf6N2E2_418 0.30 +0.1 486,669 - Pf6N2E2_418 0.30 +0.9 486,669 - Pf6N2E2_418 0.30 -2.5 487,226 + Pf6N2E2_418 0.56 +0.4 487,226 + Pf6N2E2_418 0.56 -0.7 487,226 + Pf6N2E2_418 0.56 +1.6 487,226 + Pf6N2E2_418 0.56 -1.0 487,226 + Pf6N2E2_418 0.56 +0.8 487,226 + Pf6N2E2_418 0.56 -0.7 487,226 + Pf6N2E2_418 0.56 -0.4 487,226 + Pf6N2E2_418 0.56 +1.6 487,226 + Pf6N2E2_418 0.56 +0.2 487,226 + Pf6N2E2_418 0.56 -0.9 487,227 - Pf6N2E2_418 0.56 -1.3 487,227 - Pf6N2E2_418 0.56 +0.3 487,227 - Pf6N2E2_418 0.56 -0.1 487,227 - Pf6N2E2_418 0.56 +0.1 487,227 - Pf6N2E2_418 0.56 +0.6 487,227 - Pf6N2E2_418 0.56 +0.5 487,227 - Pf6N2E2_418 0.56 -1.3 487,227 - Pf6N2E2_418 0.56 -1.0 487,227 - Pf6N2E2_418 0.56 +1.0 487,227 - Pf6N2E2_418 0.56 +1.5 487,227 - Pf6N2E2_418 0.56 -0.5 487,227 - Pf6N2E2_418 0.56 -2.4 487,227 - Pf6N2E2_418 0.56 -0.2 487,520 + Pf6N2E2_418 0.70 +1.5 487,520 + Pf6N2E2_418 0.70 -0.5 487,521 - Pf6N2E2_418 0.70 -1.9 487,521 - Pf6N2E2_418 0.70 +2.1 487,521 - Pf6N2E2_418 0.70 +0.7 487,608 - Pf6N2E2_418 0.75 -0.7 487,754 + Pf6N2E2_418 0.82 -1.8 487,754 + Pf6N2E2_418 0.82 +1.6 488,148 + -0.7 488,149 - +1.3 488,170 - +1.8 488,170 - +0.0 488,170 - -1.1 488,278 + Pf6N2E2_419 0.34 -0.6 488,278 + Pf6N2E2_419 0.34 -0.6 488,278 + Pf6N2E2_419 0.34 +0.5 488,278 + Pf6N2E2_419 0.34 -0.1 488,279 - Pf6N2E2_419 0.34 -0.9 488,279 - Pf6N2E2_419 0.34 -1.2 488,279 - Pf6N2E2_419 0.34 -1.2 488,279 - Pf6N2E2_419 0.34 +0.2 488,400 - Pf6N2E2_419 0.76 -1.6 488,400 - Pf6N2E2_419 0.76 +0.4 488,454 - +0.3 488,631 - Pf6N2E2_420 0.40 +0.2 488,631 - Pf6N2E2_420 0.40 -0.0 488,836 + -0.4 488,837 - -0.2 488,864 - -0.8 488,864 - -0.6 488,941 + -0.6 488,941 + +0.3 488,941 + +0.6 488,941 + -0.6 488,941 + -0.9 488,942 - -2.8
Or see this region's nucleotide sequence