Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_414 and Pf6N2E2_415 are separated by 17 nucleotides Pf6N2E2_415 and Pf6N2E2_416 overlap by 4 nucleotides Pf6N2E2_416 and Pf6N2E2_417 are separated by 39 nucleotides
Pf6N2E2_414 - Chemotaxis protein CheD, at 482,445 to 482,921
_414
Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775
_415
Pf6N2E2_416 - Positive regulator of CheA protein activity (CheW), at 483,772 to 484,308
_416
Pf6N2E2_417 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 484,348 to 486,006
_417
Position (kb) 483
484
485 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 482.909 kb on - strand at 482.909 kb on - strand at 482.909 kb on - strand at 483.025 kb on - strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.240 kb on + strand, within Pf6N2E2_415 at 483.241 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.393 kb on + strand, within Pf6N2E2_415 at 483.394 kb on - strand, within Pf6N2E2_415 at 483.465 kb on - strand, within Pf6N2E2_415 at 483.465 kb on - strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.588 kb on + strand, within Pf6N2E2_415 at 483.597 kb on + strand, within Pf6N2E2_415 at 483.597 kb on + strand, within Pf6N2E2_415 at 483.598 kb on - strand, within Pf6N2E2_415 at 483.612 kb on + strand, within Pf6N2E2_415 at 483.613 kb on - strand, within Pf6N2E2_415 at 483.691 kb on - strand, within Pf6N2E2_415 at 483.691 kb on - strand, within Pf6N2E2_415 at 483.859 kb on + strand, within Pf6N2E2_416 at 483.859 kb on + strand, within Pf6N2E2_416 at 484.239 kb on + strand, within Pf6N2E2_416 at 484.239 kb on + strand, within Pf6N2E2_416 at 484.240 kb on - strand, within Pf6N2E2_416 at 484.240 kb on - strand, within Pf6N2E2_416 at 484.240 kb on - strand, within Pf6N2E2_416 at 484.251 kb on - strand, within Pf6N2E2_416 at 484.251 kb on - strand, within Pf6N2E2_416 at 484.324 kb on - strand at 484.324 kb on - strand at 484.324 kb on - strand at 484.394 kb on + strand at 484.394 kb on + strand at 484.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 482,909 - -0.7 482,909 - -0.7 482,909 - -1.0 483,025 - Pf6N2E2_415 0.10 +0.3 483,090 + Pf6N2E2_415 0.18 +0.7 483,090 + Pf6N2E2_415 0.18 -1.6 483,090 + Pf6N2E2_415 0.18 -0.8 483,090 + Pf6N2E2_415 0.18 +1.2 483,091 - Pf6N2E2_415 0.18 +2.1 483,091 - Pf6N2E2_415 0.18 +0.4 483,091 - Pf6N2E2_415 0.18 -0.5 483,240 + Pf6N2E2_415 0.36 +0.6 483,241 - Pf6N2E2_415 0.36 -0.2 483,315 - Pf6N2E2_415 0.45 -0.6 483,315 - Pf6N2E2_415 0.45 -0.1 483,393 + Pf6N2E2_415 0.54 +0.7 483,394 - Pf6N2E2_415 0.54 +0.5 483,465 - Pf6N2E2_415 0.63 +1.9 483,465 - Pf6N2E2_415 0.63 -0.8 483,548 + Pf6N2E2_415 0.73 +0.5 483,548 + Pf6N2E2_415 0.73 +1.6 483,548 + Pf6N2E2_415 0.73 +0.1 483,548 + Pf6N2E2_415 0.73 -3.0 483,548 + Pf6N2E2_415 0.73 -0.3 483,548 + Pf6N2E2_415 0.73 +1.3 483,549 - Pf6N2E2_415 0.73 -1.0 483,549 - Pf6N2E2_415 0.73 +0.7 483,549 - Pf6N2E2_415 0.73 +0.9 483,588 + Pf6N2E2_415 0.78 +0.2 483,597 + Pf6N2E2_415 0.79 -0.9 483,597 + Pf6N2E2_415 0.79 -1.8 483,598 - Pf6N2E2_415 0.79 +0.6 483,612 + Pf6N2E2_415 0.80 +0.2 483,613 - Pf6N2E2_415 0.81 -3.0 483,691 - Pf6N2E2_415 0.90 +0.1 483,691 - Pf6N2E2_415 0.90 -1.6 483,859 + Pf6N2E2_416 0.16 +0.6 483,859 + Pf6N2E2_416 0.16 -0.7 484,239 + Pf6N2E2_416 0.87 -0.2 484,239 + Pf6N2E2_416 0.87 +0.4 484,240 - Pf6N2E2_416 0.87 -0.1 484,240 - Pf6N2E2_416 0.87 +0.4 484,240 - Pf6N2E2_416 0.87 -2.8 484,251 - Pf6N2E2_416 0.89 +1.4 484,251 - Pf6N2E2_416 0.89 -0.4 484,324 - -0.6 484,324 - -4.7 484,324 - -2.4 484,394 + -0.9 484,394 + -0.1 484,395 - -3.2
Or see this region's nucleotide sequence