Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4119 and Pf6N2E2_4120 are separated by 127 nucleotides Pf6N2E2_4120 and Pf6N2E2_4121 are separated by 24 nucleotides
Pf6N2E2_4119 - Chaperone protein YscY (Yop proteins translocation protein Y), at 4,798,387 to 4,798,698
_4119
Pf6N2E2_4120 - Trk system potassium uptake protein TrkA, at 4,798,826 to 4,800,199
_4120
Pf6N2E2_4121 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 4,800,224 to 4,801,534
_4121
Position (kb)
4798
4799
4800
4801 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4797.865 kb on - strand at 4797.865 kb on - strand at 4797.865 kb on - strand at 4797.958 kb on + strand at 4797.987 kb on + strand at 4797.987 kb on + strand at 4797.987 kb on + strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.988 kb on - strand at 4797.989 kb on + strand at 4797.989 kb on + strand at 4797.989 kb on + strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4797.990 kb on - strand at 4798.023 kb on - strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.040 kb on + strand at 4798.041 kb on - strand at 4798.041 kb on - strand at 4798.041 kb on - strand at 4798.130 kb on + strand at 4798.131 kb on - strand at 4798.163 kb on - strand at 4798.185 kb on + strand at 4798.244 kb on + strand at 4798.251 kb on + strand at 4798.251 kb on + strand at 4798.252 kb on - strand at 4798.252 kb on - strand at 4798.265 kb on - strand at 4798.265 kb on - strand at 4798.270 kb on + strand at 4798.270 kb on + strand at 4798.270 kb on + strand at 4798.271 kb on - strand at 4798.271 kb on - strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.274 kb on + strand at 4798.275 kb on - strand at 4798.275 kb on - strand at 4798.275 kb on - strand at 4798.278 kb on + strand at 4798.278 kb on + strand at 4798.278 kb on + strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.279 kb on - strand at 4798.284 kb on + strand at 4798.284 kb on + strand at 4798.284 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.288 kb on + strand at 4798.289 kb on - strand at 4798.289 kb on - strand at 4798.289 kb on - strand at 4798.294 kb on + strand at 4798.294 kb on + strand at 4798.295 kb on - strand at 4798.332 kb on + strand at 4798.501 kb on + strand, within Pf6N2E2_4119 at 4798.501 kb on + strand, within Pf6N2E2_4119 at 4798.502 kb on - strand, within Pf6N2E2_4119 at 4798.534 kb on + strand, within Pf6N2E2_4119 at 4798.535 kb on - strand, within Pf6N2E2_4119 at 4798.553 kb on + strand at 4798.553 kb on + strand, within Pf6N2E2_4119 at 4798.553 kb on + strand, within Pf6N2E2_4119 at 4798.554 kb on - strand, within Pf6N2E2_4119 at 4798.554 kb on - strand, within Pf6N2E2_4119 at 4798.622 kb on + strand, within Pf6N2E2_4119 at 4798.633 kb on + strand, within Pf6N2E2_4119 at 4798.656 kb on + strand, within Pf6N2E2_4119 at 4799.398 kb on - strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.552 kb on - strand, within Pf6N2E2_4120 at 4799.719 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4800.261 kb on + strand at 4800.262 kb on - strand at 4800.262 kb on - strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.436 kb on + strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.933 kb on + strand, within Pf6N2E2_4121 at 4800.934 kb on - strand, within Pf6N2E2_4121 at 4800.982 kb on - strand, within Pf6N2E2_4121
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,797,865 - -0.1 4,797,865 - +1.2 4,797,865 - -1.3 4,797,958 + -2.8 4,797,987 + +1.1 4,797,987 + -1.4 4,797,987 + -1.3 4,797,988 - +0.4 4,797,988 - -1.4 4,797,988 - +1.3 4,797,988 - -2.4 4,797,988 - -1.1 4,797,988 - -1.1 4,797,989 + +3.4 4,797,989 + -0.7 4,797,989 + +0.4 4,797,990 - -0.1 4,797,990 - +0.4 4,797,990 - -0.2 4,797,990 - -2.3 4,797,990 - +0.1 4,797,990 - -1.4 4,797,990 - -0.5 4,797,990 - -0.9 4,797,990 - +0.8 4,797,990 - +0.2 4,798,023 - +0.2 4,798,040 + +1.2 4,798,040 + +0.2 4,798,040 + +0.9 4,798,040 + -1.1 4,798,041 - +0.4 4,798,041 - -0.8 4,798,041 - +0.8 4,798,130 + +0.3 4,798,131 - +0.7 4,798,163 - +1.5 4,798,185 + +0.7 4,798,244 + +0.7 4,798,251 + -1.0 4,798,251 + -0.0 4,798,252 - +0.3 4,798,252 - -0.6 4,798,265 - -2.5 4,798,265 - -0.5 4,798,270 + +1.4 4,798,270 + -0.5 4,798,270 + +0.6 4,798,271 - +0.7 4,798,271 - -1.2 4,798,274 + +0.1 4,798,274 + -1.8 4,798,274 + -1.5 4,798,274 + -1.0 4,798,274 + +0.3 4,798,275 - -0.2 4,798,275 - -0.1 4,798,275 - +1.0 4,798,278 + +1.4 4,798,278 + +0.3 4,798,278 + +0.1 4,798,279 - +0.5 4,798,279 - -1.6 4,798,279 - -0.4 4,798,279 - +0.3 4,798,279 - -1.0 4,798,279 - -3.0 4,798,279 - +0.8 4,798,279 - +0.9 4,798,279 - -0.7 4,798,279 - +1.7 4,798,279 - +2.0 4,798,279 - -1.3 4,798,279 - +2.3 4,798,279 - -0.3 4,798,284 + -1.3 4,798,284 + -0.3 4,798,284 + -0.4 4,798,288 + +0.6 4,798,288 + -0.0 4,798,288 + +0.4 4,798,288 + +1.9 4,798,289 - +1.0 4,798,289 - +1.2 4,798,289 - -0.3 4,798,294 + +0.4 4,798,294 + +0.6 4,798,295 - +0.1 4,798,332 + +0.3 4,798,501 + Pf6N2E2_4119 0.37 -0.0 4,798,501 + Pf6N2E2_4119 0.37 +0.4 4,798,502 - Pf6N2E2_4119 0.37 -1.4 4,798,534 + Pf6N2E2_4119 0.47 +0.4 4,798,535 - Pf6N2E2_4119 0.47 +0.5 4,798,553 + +2.1 4,798,553 + Pf6N2E2_4119 0.53 -0.7 4,798,553 + Pf6N2E2_4119 0.53 -0.6 4,798,554 - Pf6N2E2_4119 0.54 -0.8 4,798,554 - Pf6N2E2_4119 0.54 +0.3 4,798,622 + Pf6N2E2_4119 0.75 -0.2 4,798,633 + Pf6N2E2_4119 0.79 -0.6 4,798,656 + Pf6N2E2_4119 0.86 -2.9 4,799,398 - Pf6N2E2_4120 0.42 -1.4 4,799,508 + Pf6N2E2_4120 0.50 -2.5 4,799,508 + Pf6N2E2_4120 0.50 -2.4 4,799,508 + Pf6N2E2_4120 0.50 -1.3 4,799,508 + Pf6N2E2_4120 0.50 -3.2 4,799,508 + Pf6N2E2_4120 0.50 -2.3 4,799,508 + Pf6N2E2_4120 0.50 -1.0 4,799,509 - Pf6N2E2_4120 0.50 -1.8 4,799,509 - Pf6N2E2_4120 0.50 -0.6 4,799,509 - Pf6N2E2_4120 0.50 -0.8 4,799,509 - Pf6N2E2_4120 0.50 -1.1 4,799,509 - Pf6N2E2_4120 0.50 -1.2 4,799,509 - Pf6N2E2_4120 0.50 -2.4 4,799,509 - Pf6N2E2_4120 0.50 +0.6 4,799,509 - Pf6N2E2_4120 0.50 -1.7 4,799,509 - Pf6N2E2_4120 0.50 -2.0 4,799,509 - Pf6N2E2_4120 0.50 -1.4 4,799,552 - Pf6N2E2_4120 0.53 -0.3 4,799,719 - Pf6N2E2_4120 0.65 -2.1 4,799,722 - Pf6N2E2_4120 0.65 -1.1 4,799,722 - Pf6N2E2_4120 0.65 +0.3 4,799,722 - Pf6N2E2_4120 0.65 -1.2 4,799,806 + Pf6N2E2_4120 0.71 -3.4 4,799,806 + Pf6N2E2_4120 0.71 -1.1 4,799,806 + Pf6N2E2_4120 0.71 -1.8 4,799,806 + Pf6N2E2_4120 0.71 -2.2 4,799,807 - Pf6N2E2_4120 0.71 -1.7 4,799,807 - Pf6N2E2_4120 0.71 -2.6 4,799,807 - Pf6N2E2_4120 0.71 -2.3 4,799,807 - Pf6N2E2_4120 0.71 -2.4 4,799,807 - Pf6N2E2_4120 0.71 -3.2 4,799,808 + Pf6N2E2_4120 0.71 -1.2 4,799,808 + Pf6N2E2_4120 0.71 -0.1 4,799,808 + Pf6N2E2_4120 0.71 -1.4 4,799,808 + Pf6N2E2_4120 0.71 -3.4 4,799,808 + Pf6N2E2_4120 0.71 -2.3 4,799,808 + Pf6N2E2_4120 0.71 -0.3 4,799,808 + Pf6N2E2_4120 0.71 -0.7 4,799,808 + Pf6N2E2_4120 0.71 -1.0 4,799,808 + Pf6N2E2_4120 0.71 -1.9 4,799,808 + Pf6N2E2_4120 0.71 -0.0 4,799,808 + Pf6N2E2_4120 0.71 -2.8 4,799,808 + Pf6N2E2_4120 0.71 -2.5 4,799,808 + Pf6N2E2_4120 0.71 -1.1 4,799,808 + Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -1.2 4,799,808 + Pf6N2E2_4120 0.71 -1.2 4,799,808 + Pf6N2E2_4120 0.71 +1.5 4,799,808 + Pf6N2E2_4120 0.71 -1.9 4,799,808 + Pf6N2E2_4120 0.71 -2.3 4,799,809 - Pf6N2E2_4120 0.72 -1.5 4,799,809 - Pf6N2E2_4120 0.72 -0.5 4,799,809 - Pf6N2E2_4120 0.72 -2.1 4,799,809 - Pf6N2E2_4120 0.72 -2.3 4,799,809 - Pf6N2E2_4120 0.72 -1.7 4,799,809 - Pf6N2E2_4120 0.72 -2.9 4,799,809 - Pf6N2E2_4120 0.72 -2.5 4,799,809 - Pf6N2E2_4120 0.72 -1.6 4,799,809 - Pf6N2E2_4120 0.72 -2.6 4,799,809 - Pf6N2E2_4120 0.72 -0.5 4,799,809 - Pf6N2E2_4120 0.72 -3.1 4,799,809 - Pf6N2E2_4120 0.72 -2.1 4,799,809 - Pf6N2E2_4120 0.72 -1.4 4,799,809 - Pf6N2E2_4120 0.72 -1.4 4,799,809 - Pf6N2E2_4120 0.72 +0.6 4,799,809 - Pf6N2E2_4120 0.72 -2.1 4,799,809 - Pf6N2E2_4120 0.72 -3.0 4,799,809 - Pf6N2E2_4120 0.72 -2.9 4,800,261 + -2.2 4,800,262 - -0.8 4,800,262 - +0.2 4,800,264 + +0.0 4,800,264 + -2.8 4,800,264 + -2.4 4,800,264 + -2.2 4,800,265 - -2.1 4,800,265 - -1.6 4,800,265 - -0.9 4,800,265 - -1.2 4,800,436 + Pf6N2E2_4121 0.16 -0.8 4,800,437 - Pf6N2E2_4121 0.16 +0.4 4,800,437 - Pf6N2E2_4121 0.16 -1.4 4,800,437 - Pf6N2E2_4121 0.16 -0.7 4,800,437 - Pf6N2E2_4121 0.16 -0.7 4,800,933 + Pf6N2E2_4121 0.54 -2.2 4,800,934 - Pf6N2E2_4121 0.54 +0.8 4,800,982 - Pf6N2E2_4121 0.58 -0.7
Or see this region's nucleotide sequence