Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3992

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
4,660,332	+		Pf6N2E2_3990	0.88	-1.5
4,660,333	-		Pf6N2E2_3990	0.88	-2.0
4,660,475	+				+0.1
4,660,475	+				+0.2
4,660,476	-				+1.6
4,660,484	+				+0.6
4,660,484	+				-1.0
4,660,484	+				-0.1
4,660,485	-				-0.5
4,660,485	-				-1.0
4,660,485	-				-0.2
4,660,485	-				-1.4
4,660,485	-				-1.3
4,660,485	-				+1.2
4,660,555	+				-2.6
4,660,555	+				+0.0
4,660,577	+				+2.6
4,660,577	+				-0.6
4,660,578	-				+0.8
4,660,621	+				-0.2
4,660,621	+				-1.1
4,660,621	+				-2.2
4,660,622	-				+1.2
4,660,622	-				+1.1
4,660,643	+				+0.6
4,660,644	-				+0.8
4,660,666	+				+1.6
4,660,666	+				-0.9
4,660,666	+				-1.4
4,660,666	+				+0.1
4,660,666	+				-0.7
4,660,667	-				+0.6
4,660,667	-				+0.9
4,660,667	-				+0.4
4,660,667	-				+0.7
4,660,667	-				+1.3
4,660,667	-				-2.5
4,660,667	-				+2.2
4,660,667	-				+2.2
4,660,667	-				+1.7
4,660,688	+				-0.3
4,660,689	-				-1.1
4,660,689	-				+0.5
4,660,689	-				+2.3
4,660,694	+				-0.1
4,660,694	+				-1.2
4,660,695	-				+1.4
4,660,695	-				+2.3
4,660,695	-				+1.9
4,660,699	-				+0.3
4,660,784	-				+2.3
4,660,840	-				+1.3
4,660,850	+				+1.2
4,660,850	+				+0.4
4,660,850	+				+0.6
4,660,851	-				+2.0
4,660,851	-				+0.3
4,660,876	+		Pf6N2E2_3991	0.12	-2.4
4,660,876	+		Pf6N2E2_3991	0.12	-0.2
4,660,876	+		Pf6N2E2_3991	0.12	+0.3
4,660,877	-		Pf6N2E2_3991	0.12	-0.8
4,661,057	+		Pf6N2E2_3991	0.48	+2.0
4,661,057	+		Pf6N2E2_3991	0.48	+1.8
4,661,057	+		Pf6N2E2_3991	0.48	-0.4
4,661,058	-		Pf6N2E2_3991	0.48	+1.4
4,661,058	-		Pf6N2E2_3991	0.48	+2.7
4,661,070	+		Pf6N2E2_3991	0.50	-3.0
4,661,070	+		Pf6N2E2_3991	0.50	+0.3
4,661,071	-		Pf6N2E2_3991	0.51	+0.3
4,661,071	-		Pf6N2E2_3991	0.51	+1.5
4,661,071	-		Pf6N2E2_3991	0.51	-0.7
4,661,071	-		Pf6N2E2_3991	0.51	+3.1
4,661,071	-		Pf6N2E2_3991	0.51	+1.9
4,661,071	-		Pf6N2E2_3991	0.51	+1.5
4,661,216	+		Pf6N2E2_3991	0.80	-2.8
4,661,216	+		Pf6N2E2_3991	0.80	+1.1
4,661,217	-		Pf6N2E2_3991	0.80	+2.3
4,661,217	-		Pf6N2E2_3991	0.80	+3.4
4,661,217	-		Pf6N2E2_3991	0.80	-1.5
4,661,217	-		Pf6N2E2_3991	0.80	+0.1
4,661,268	-				+1.2
4,661,285	+				+0.2
4,661,285	+				-0.3
4,661,286	-				-0.2
4,661,286	-				+0.3
4,661,294	+				-0.4
4,661,294	+				+0.7
4,661,294	+				-0.4
4,661,294	+				-0.1
4,661,294	+				-0.8
4,661,294	+				+0.1
4,661,294	+				-0.2
4,661,294	+				-1.0
4,661,294	+				+0.3
4,661,294	+				-0.7
4,661,294	+				+0.8
4,661,295	-				+1.6
4,661,295	-				+1.5
4,661,295	-				+0.4
4,661,295	-				+1.5
4,661,295	-				+2.5
4,662,015	+		Pf6N2E2_3992	0.71	+0.6
4,662,015	+		Pf6N2E2_3992	0.71	+1.4
4,662,015	+		Pf6N2E2_3992	0.71	+0.1
4,662,016	-		Pf6N2E2_3992	0.71	-1.1
4,662,133	+		Pf6N2E2_3992	0.82	+1.2
4,662,133	+		Pf6N2E2_3992	0.82	+0.1
4,662,133	+		Pf6N2E2_3992	0.82	+1.5
4,662,134	-		Pf6N2E2_3992	0.83	+0.0
4,662,134	-		Pf6N2E2_3992	0.83	+0.9
4,662,134	-		Pf6N2E2_3992	0.83	+1.6
4,662,134	-		Pf6N2E2_3992	0.83	-1.3
4,662,134	-		Pf6N2E2_3992	0.83	+0.8
4,662,153	+		Pf6N2E2_3992	0.84	+1.0
4,662,155	+		Pf6N2E2_3992	0.85	+0.3
4,662,155	+		Pf6N2E2_3992	0.85	-2.6
4,662,155	+		Pf6N2E2_3992	0.85	-1.8
4,662,155	+		Pf6N2E2_3992	0.85	+0.8
4,662,156	-		Pf6N2E2_3992	0.85	+0.4
4,662,156	-		Pf6N2E2_3992	0.85	+1.5
4,662,156	-		Pf6N2E2_3992	0.85	-1.1
4,662,156	-		Pf6N2E2_3992	0.85	+0.1
4,662,156	-		Pf6N2E2_3992	0.85	+1.7
4,662,156	-		Pf6N2E2_3992	0.85	+0.4
4,662,156	-		Pf6N2E2_3992	0.85	+0.0
4,662,350	+		Pf6N2E2_3993	0.21	+0.7
4,662,350	+		Pf6N2E2_3993	0.21	+1.5
4,662,350	+		Pf6N2E2_3993	0.21	-0.6
4,662,350	+		Pf6N2E2_3993	0.21	-1.8
4,662,351	-		Pf6N2E2_3993	0.22	+1.8
4,662,351	-		Pf6N2E2_3993	0.22	+0.7
4,662,361	+		Pf6N2E2_3993	0.31	+0.8
4,662,361	+		Pf6N2E2_3993	0.31	+0.3
4,662,362	-		Pf6N2E2_3993	0.32	-1.2
4,662,362	-		Pf6N2E2_3993	0.32	-1.1
4,662,362	-		Pf6N2E2_3993	0.32	-2.8
4,662,390	+		Pf6N2E2_3993	0.56	-0.8
4,662,390	+		Pf6N2E2_3993	0.56	-1.6
4,662,390	+		Pf6N2E2_3993	0.56	-0.1
4,662,390	+		Pf6N2E2_3993	0.56	-0.0
4,662,390	+		Pf6N2E2_3993	0.56	-0.9
4,662,391	-		Pf6N2E2_3993	0.56	-1.6
4,662,391	-		Pf6N2E2_3993	0.56	+1.2
4,662,391	-		Pf6N2E2_3993	0.56	+0.5
4,662,391	-		Pf6N2E2_3993	0.56	-0.2
4,662,407	+				-0.6
4,662,407	+				-0.1
4,662,407	+				-1.4
4,662,560	-				+1.2
4,662,560	-				+0.6
4,662,682	+		Pf6N2E2_3994	0.11	-1.9
4,662,757	+		Pf6N2E2_3994	0.14	-0.4
4,662,757	+		Pf6N2E2_3994	0.14	-1.2
4,662,758	-		Pf6N2E2_3994	0.15	+0.1
4,662,758	-		Pf6N2E2_3994	0.15	+0.2
4,662,758	-		Pf6N2E2_3994	0.15	-1.2
4,662,758	-		Pf6N2E2_3994	0.15	-0.4
4,662,856	+		Pf6N2E2_3994	0.18	+1.0
4,662,988	+		Pf6N2E2_3994	0.24	+0.3
4,662,989	-		Pf6N2E2_3994	0.24	-0.2
4,662,989	-		Pf6N2E2_3994	0.24	-0.2
4,663,039	+		Pf6N2E2_3994	0.26	+1.0
4,663,039	+		Pf6N2E2_3994	0.26	-1.9
4,663,039	+		Pf6N2E2_3994	0.26	-0.8
4,663,039	+		Pf6N2E2_3994	0.26	+0.6
4,663,039	+		Pf6N2E2_3994	0.26	-0.0
4,663,039	+		Pf6N2E2_3994	0.26	+0.1
4,663,039	+		Pf6N2E2_3994	0.26	+0.3
4,663,040	-		Pf6N2E2_3994	0.26	-1.1
4,663,040	-		Pf6N2E2_3994	0.26	-0.8
4,663,077	+		Pf6N2E2_3994	0.27	-0.9
4,663,084	+		Pf6N2E2_3994	0.28	-1.7
4,663,084	+		Pf6N2E2_3994	0.28	-0.0
4,663,084	+		Pf6N2E2_3994	0.28	-1.6
4,663,084	+		Pf6N2E2_3994	0.28	-1.9
4,663,085	-		Pf6N2E2_3994	0.28	-1.6
4,663,085	-		Pf6N2E2_3994	0.28	+0.2
4,663,123	+		Pf6N2E2_3994	0.29	-2.4
4,663,123	+		Pf6N2E2_3994	0.29	+0.0
4,663,123	+		Pf6N2E2_3994	0.29	-0.5
4,663,123	+		Pf6N2E2_3994	0.29	+0.4
4,663,123	+		Pf6N2E2_3994	0.29	+0.6
4,663,123	+		Pf6N2E2_3994	0.29	+0.5
4,663,123	+		Pf6N2E2_3994	0.29	+0.6
4,663,123	+		Pf6N2E2_3994	0.29	+0.9
4,663,124	-		Pf6N2E2_3994	0.29	-1.0
4,663,124	-		Pf6N2E2_3994	0.29	+0.2
4,663,124	-		Pf6N2E2_3994	0.29	+1.5
4,663,124	-		Pf6N2E2_3994	0.29	+0.7
4,663,124	-		Pf6N2E2_3994	0.29	+0.2
4,663,124	-		Pf6N2E2_3994	0.29	+0.1
4,663,124	-		Pf6N2E2_3994	0.29	+1.3
4,663,124	-		Pf6N2E2_3994	0.29	+0.8
4,663,148	-		Pf6N2E2_3994	0.30	+0.1
4,663,148	-		Pf6N2E2_3994	0.30	+0.4
4,663,196	-		Pf6N2E2_3994	0.32	-0.9
4,663,196	-		Pf6N2E2_3994	0.32	-0.9
4,663,249	+		Pf6N2E2_3994	0.34	-1.6
4,663,249	+		Pf6N2E2_3994	0.34	+1.2