Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3938 and Pf6N2E2_3939 are separated by 144 nucleotides Pf6N2E2_3939 and Pf6N2E2_3940 are separated by 37 nucleotides Pf6N2E2_3940 and Pf6N2E2_3941 are separated by 143 nucleotides
Pf6N2E2_3938 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,574,951 to 4,576,465
_3938
Pf6N2E2_3939 - Predicted integral membrane protein, at 4,576,610 to 4,577,035
_3939
Pf6N2E2_3940 - Phosphoserine phosphatase (EC 3.1.3.3), at 4,577,073 to 4,577,729
_3940
Pf6N2E2_3941 - Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-), at 4,577,873 to 4,578,352
_3941
Position (kb)
4576
4577
4578 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.692 kb on + strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.693 kb on - strand, within Pf6N2E2_3938 at 4575.749 kb on - strand, within Pf6N2E2_3938 at 4575.749 kb on - strand, within Pf6N2E2_3938 at 4575.815 kb on + strand, within Pf6N2E2_3938 at 4576.025 kb on + strand, within Pf6N2E2_3938 at 4576.026 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.032 kb on - strand, within Pf6N2E2_3938 at 4576.435 kb on + strand at 4576.445 kb on + strand at 4576.445 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.574 kb on + strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.575 kb on - strand at 4576.596 kb on - strand at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.877 kb on + strand, within Pf6N2E2_3939 at 4576.878 kb on - strand, within Pf6N2E2_3939 at 4576.878 kb on - strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.879 kb on + strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.880 kb on - strand, within Pf6N2E2_3939 at 4576.961 kb on - strand, within Pf6N2E2_3939 at 4576.961 kb on - strand, within Pf6N2E2_3939 at 4577.065 kb on + strand at 4577.201 kb on - strand, within Pf6N2E2_3940 at 4577.201 kb on - strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.206 kb on + strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.207 kb on - strand, within Pf6N2E2_3940 at 4577.306 kb on - strand, within Pf6N2E2_3940 at 4577.491 kb on + strand, within Pf6N2E2_3940 at 4577.491 kb on + strand, within Pf6N2E2_3940 at 4577.491 kb on + strand, within Pf6N2E2_3940 at 4577.491 kb on + strand, within Pf6N2E2_3940 at 4577.492 kb on - strand, within Pf6N2E2_3940 at 4577.560 kb on + strand, within Pf6N2E2_3940 at 4577.560 kb on + strand, within Pf6N2E2_3940 at 4577.590 kb on + strand, within Pf6N2E2_3940 at 4577.591 kb on - strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.623 kb on + strand, within Pf6N2E2_3940 at 4577.624 kb on - strand, within Pf6N2E2_3940 at 4577.624 kb on - strand, within Pf6N2E2_3940 at 4577.624 kb on - strand, within Pf6N2E2_3940 at 4577.624 kb on - strand, within Pf6N2E2_3940 at 4577.659 kb on + strand, within Pf6N2E2_3940 at 4577.660 kb on - strand, within Pf6N2E2_3940 at 4577.660 kb on - strand, within Pf6N2E2_3940 at 4577.715 kb on + strand at 4577.736 kb on + strand at 4577.737 kb on - strand at 4577.796 kb on + strand at 4577.796 kb on + strand at 4577.796 kb on + strand at 4577.796 kb on + strand at 4577.796 kb on + strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.797 kb on - strand at 4577.801 kb on + strand at 4577.802 kb on - strand at 4577.918 kb on - strand at 4577.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,575,692 + Pf6N2E2_3938 0.49 +0.3 4,575,692 + Pf6N2E2_3938 0.49 +0.2 4,575,692 + Pf6N2E2_3938 0.49 -1.7 4,575,692 + Pf6N2E2_3938 0.49 +0.2 4,575,692 + Pf6N2E2_3938 0.49 +0.9 4,575,692 + Pf6N2E2_3938 0.49 -0.5 4,575,692 + Pf6N2E2_3938 0.49 -1.5 4,575,692 + Pf6N2E2_3938 0.49 +2.1 4,575,692 + Pf6N2E2_3938 0.49 +1.0 4,575,692 + Pf6N2E2_3938 0.49 -0.4 4,575,693 - Pf6N2E2_3938 0.49 -0.2 4,575,693 - Pf6N2E2_3938 0.49 +0.4 4,575,693 - Pf6N2E2_3938 0.49 -0.8 4,575,693 - Pf6N2E2_3938 0.49 +1.1 4,575,693 - Pf6N2E2_3938 0.49 +1.3 4,575,749 - Pf6N2E2_3938 0.53 -1.9 4,575,749 - Pf6N2E2_3938 0.53 -0.6 4,575,815 + Pf6N2E2_3938 0.57 -2.6 4,576,025 + Pf6N2E2_3938 0.71 +0.2 4,576,026 - Pf6N2E2_3938 0.71 +1.1 4,576,032 - Pf6N2E2_3938 0.71 +0.5 4,576,032 - Pf6N2E2_3938 0.71 -0.4 4,576,032 - Pf6N2E2_3938 0.71 -1.0 4,576,435 + -2.2 4,576,445 + -2.2 4,576,445 + -1.7 4,576,574 + -0.4 4,576,574 + -1.5 4,576,574 + -1.6 4,576,574 + -0.3 4,576,574 + +0.1 4,576,574 + +0.1 4,576,575 - +0.1 4,576,575 - -1.6 4,576,575 - +1.5 4,576,575 - +0.2 4,576,575 - -1.3 4,576,575 - +1.2 4,576,575 - -1.2 4,576,596 - -0.5 4,576,877 + Pf6N2E2_3939 0.63 +1.1 4,576,877 + Pf6N2E2_3939 0.63 -0.3 4,576,877 + Pf6N2E2_3939 0.63 +0.5 4,576,877 + Pf6N2E2_3939 0.63 +0.5 4,576,878 - Pf6N2E2_3939 0.63 +1.2 4,576,878 - Pf6N2E2_3939 0.63 +0.4 4,576,879 + Pf6N2E2_3939 0.63 +0.9 4,576,879 + Pf6N2E2_3939 0.63 -2.2 4,576,879 + Pf6N2E2_3939 0.63 +1.6 4,576,879 + Pf6N2E2_3939 0.63 +0.2 4,576,879 + Pf6N2E2_3939 0.63 +0.1 4,576,880 - Pf6N2E2_3939 0.63 -0.2 4,576,880 - Pf6N2E2_3939 0.63 +0.9 4,576,880 - Pf6N2E2_3939 0.63 -1.9 4,576,880 - Pf6N2E2_3939 0.63 -1.4 4,576,880 - Pf6N2E2_3939 0.63 +0.6 4,576,961 - Pf6N2E2_3939 0.82 +3.4 4,576,961 - Pf6N2E2_3939 0.82 -0.3 4,577,065 + +1.2 4,577,201 - Pf6N2E2_3940 0.19 +0.3 4,577,201 - Pf6N2E2_3940 0.19 +0.5 4,577,206 + Pf6N2E2_3940 0.20 -2.5 4,577,206 + Pf6N2E2_3940 0.20 -0.9 4,577,206 + Pf6N2E2_3940 0.20 +1.1 4,577,207 - Pf6N2E2_3940 0.20 +0.6 4,577,207 - Pf6N2E2_3940 0.20 +0.2 4,577,207 - Pf6N2E2_3940 0.20 +2.1 4,577,207 - Pf6N2E2_3940 0.20 -0.0 4,577,306 - Pf6N2E2_3940 0.35 +0.6 4,577,491 + Pf6N2E2_3940 0.64 -1.2 4,577,491 + Pf6N2E2_3940 0.64 -1.1 4,577,491 + Pf6N2E2_3940 0.64 -1.1 4,577,491 + Pf6N2E2_3940 0.64 -1.4 4,577,492 - Pf6N2E2_3940 0.64 -0.1 4,577,560 + Pf6N2E2_3940 0.74 -1.2 4,577,560 + Pf6N2E2_3940 0.74 -2.4 4,577,590 + Pf6N2E2_3940 0.79 +1.4 4,577,591 - Pf6N2E2_3940 0.79 -1.3 4,577,623 + Pf6N2E2_3940 0.84 +0.1 4,577,623 + Pf6N2E2_3940 0.84 +1.1 4,577,623 + Pf6N2E2_3940 0.84 +1.9 4,577,623 + Pf6N2E2_3940 0.84 -2.7 4,577,623 + Pf6N2E2_3940 0.84 +0.4 4,577,623 + Pf6N2E2_3940 0.84 -0.4 4,577,623 + Pf6N2E2_3940 0.84 -1.0 4,577,623 + Pf6N2E2_3940 0.84 -0.2 4,577,623 + Pf6N2E2_3940 0.84 -1.3 4,577,623 + Pf6N2E2_3940 0.84 -0.1 4,577,623 + Pf6N2E2_3940 0.84 -0.3 4,577,623 + Pf6N2E2_3940 0.84 +0.5 4,577,624 - Pf6N2E2_3940 0.84 +0.4 4,577,624 - Pf6N2E2_3940 0.84 +0.6 4,577,624 - Pf6N2E2_3940 0.84 -0.5 4,577,624 - Pf6N2E2_3940 0.84 +0.9 4,577,659 + Pf6N2E2_3940 0.89 -0.1 4,577,660 - Pf6N2E2_3940 0.89 +1.1 4,577,660 - Pf6N2E2_3940 0.89 -0.0 4,577,715 + -0.7 4,577,736 + +0.7 4,577,737 - -0.4 4,577,796 + -2.6 4,577,796 + -1.9 4,577,796 + -1.7 4,577,796 + -2.0 4,577,796 + -0.7 4,577,797 - +2.1 4,577,797 - -1.1 4,577,797 - +2.9 4,577,797 - +1.0 4,577,797 - +1.6 4,577,797 - +1.1 4,577,797 - -1.2 4,577,797 - +3.9 4,577,801 + -1.6 4,577,802 - -1.1 4,577,918 - -0.6 4,577,918 - +0.4
Or see this region's nucleotide sequence