Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3790 and Pf6N2E2_3791 are separated by 201 nucleotides Pf6N2E2_3791 and Pf6N2E2_3792 are separated by 49 nucleotides
Pf6N2E2_3790 - hypothetical protein, at 4,414,474 to 4,414,815
_3790
Pf6N2E2_3791 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 4,415,017 to 4,417,530
_3791
Pf6N2E2_3792 - Glucans biosynthesis protein G precursor, at 4,417,580 to 4,419,322
_3792
Position (kb)
4415
4416
4417
4418 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4414.082 kb on + strand at 4414.082 kb on + strand at 4414.083 kb on - strand at 4414.083 kb on - strand at 4414.110 kb on - strand at 4414.123 kb on + strand at 4414.138 kb on + strand at 4414.138 kb on + strand at 4414.139 kb on - strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.151 kb on + strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.152 kb on - strand at 4414.155 kb on + strand at 4414.155 kb on + strand at 4414.155 kb on + strand at 4414.156 kb on - strand at 4414.156 kb on - strand at 4414.156 kb on - strand at 4414.156 kb on - strand at 4414.156 kb on - strand at 4414.217 kb on + strand at 4414.217 kb on + strand at 4414.217 kb on + strand at 4414.217 kb on + strand at 4414.217 kb on + strand at 4414.217 kb on + strand at 4414.218 kb on - strand at 4414.218 kb on - strand at 4414.218 kb on - strand at 4414.223 kb on + strand at 4414.223 kb on + strand at 4414.224 kb on - strand at 4414.266 kb on - strand at 4414.319 kb on + strand at 4414.320 kb on - strand at 4414.431 kb on - strand at 4414.431 kb on - strand at 4414.555 kb on + strand, within Pf6N2E2_3790 at 4414.556 kb on - strand, within Pf6N2E2_3790 at 4414.556 kb on - strand, within Pf6N2E2_3790 at 4414.558 kb on + strand, within Pf6N2E2_3790 at 4414.558 kb on + strand, within Pf6N2E2_3790 at 4414.743 kb on + strand, within Pf6N2E2_3790 at 4414.743 kb on + strand, within Pf6N2E2_3790 at 4414.744 kb on - strand, within Pf6N2E2_3790 at 4414.744 kb on - strand, within Pf6N2E2_3790 at 4414.744 kb on - strand, within Pf6N2E2_3790 at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.805 kb on + strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.806 kb on - strand at 4414.929 kb on + strand at 4415.295 kb on - strand, within Pf6N2E2_3791 at 4415.375 kb on + strand, within Pf6N2E2_3791 at 4415.375 kb on + strand, within Pf6N2E2_3791 at 4415.375 kb on + strand, within Pf6N2E2_3791 at 4415.375 kb on + strand, within Pf6N2E2_3791 at 4415.375 kb on + strand, within Pf6N2E2_3791 at 4415.376 kb on - strand, within Pf6N2E2_3791 at 4415.376 kb on - strand, within Pf6N2E2_3791 at 4415.412 kb on - strand, within Pf6N2E2_3791 at 4415.412 kb on - strand, within Pf6N2E2_3791 at 4415.821 kb on - strand, within Pf6N2E2_3791 at 4415.933 kb on + strand, within Pf6N2E2_3791 at 4415.933 kb on + strand, within Pf6N2E2_3791 at 4416.014 kb on + strand, within Pf6N2E2_3791 at 4416.014 kb on + strand, within Pf6N2E2_3791 at 4416.015 kb on - strand, within Pf6N2E2_3791 at 4416.176 kb on + strand, within Pf6N2E2_3791 at 4416.422 kb on + strand, within Pf6N2E2_3791 at 4416.422 kb on + strand, within Pf6N2E2_3791 at 4416.423 kb on - strand, within Pf6N2E2_3791 at 4416.423 kb on - strand, within Pf6N2E2_3791 at 4416.423 kb on - strand, within Pf6N2E2_3791 at 4416.517 kb on - strand, within Pf6N2E2_3791 at 4416.554 kb on + strand, within Pf6N2E2_3791 at 4416.554 kb on + strand, within Pf6N2E2_3791 at 4416.554 kb on + strand, within Pf6N2E2_3791 at 4416.554 kb on + strand, within Pf6N2E2_3791 at 4416.554 kb on + strand, within Pf6N2E2_3791 at 4416.555 kb on - strand, within Pf6N2E2_3791 at 4416.560 kb on + strand, within Pf6N2E2_3791 at 4416.561 kb on - strand, within Pf6N2E2_3791 at 4416.561 kb on - strand, within Pf6N2E2_3791 at 4416.635 kb on + strand, within Pf6N2E2_3791 at 4416.705 kb on + strand, within Pf6N2E2_3791 at 4416.717 kb on + strand, within Pf6N2E2_3791 at 4416.718 kb on - strand, within Pf6N2E2_3791 at 4416.811 kb on + strand, within Pf6N2E2_3791 at 4416.812 kb on - strand, within Pf6N2E2_3791 at 4416.900 kb on - strand, within Pf6N2E2_3791 at 4417.091 kb on + strand, within Pf6N2E2_3791 at 4417.113 kb on - strand, within Pf6N2E2_3791 at 4417.160 kb on + strand, within Pf6N2E2_3791 at 4417.182 kb on + strand, within Pf6N2E2_3791 at 4417.183 kb on - strand, within Pf6N2E2_3791 at 4417.547 kb on + strand at 4417.547 kb on + strand at 4417.547 kb on + strand at 4417.548 kb on - strand at 4417.548 kb on - strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.578 kb on + strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.579 kb on - strand at 4417.581 kb on + strand at 4417.581 kb on + strand at 4417.581 kb on + strand at 4417.581 kb on + strand at 4417.582 kb on - strand at 4417.582 kb on - strand at 4417.582 kb on - strand at 4417.603 kb on - strand at 4417.603 kb on - strand at 4417.917 kb on + strand, within Pf6N2E2_3792 at 4417.918 kb on - strand, within Pf6N2E2_3792 at 4418.029 kb on - strand, within Pf6N2E2_3792 at 4418.029 kb on - strand, within Pf6N2E2_3792 at 4418.029 kb on - strand, within Pf6N2E2_3792 at 4418.029 kb on - strand, within Pf6N2E2_3792 at 4418.344 kb on - strand, within Pf6N2E2_3792 at 4418.509 kb on - strand, within Pf6N2E2_3792 at 4418.509 kb on - strand, within Pf6N2E2_3792 at 4418.509 kb on - strand, within Pf6N2E2_3792
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,414,082 + -0.8 4,414,082 + -0.7 4,414,083 - +0.6 4,414,083 - +1.6 4,414,110 - +1.7 4,414,123 + -0.2 4,414,138 + -1.6 4,414,138 + +0.3 4,414,139 - +1.6 4,414,151 + -1.4 4,414,151 + -0.1 4,414,151 + -2.6 4,414,151 + +0.6 4,414,151 + -0.7 4,414,151 + -0.3 4,414,151 + -0.5 4,414,151 + -1.7 4,414,152 - +0.3 4,414,152 - -0.0 4,414,152 - -0.4 4,414,152 - +0.1 4,414,152 - +0.5 4,414,152 - -1.3 4,414,152 - -0.0 4,414,152 - +0.7 4,414,155 + -1.5 4,414,155 + -1.0 4,414,155 + -0.1 4,414,156 - +1.7 4,414,156 - -0.5 4,414,156 - +1.2 4,414,156 - -1.3 4,414,156 - +1.3 4,414,217 + -1.3 4,414,217 + -0.1 4,414,217 + -0.1 4,414,217 + +1.0 4,414,217 + -1.3 4,414,217 + +1.2 4,414,218 - -0.0 4,414,218 - -0.3 4,414,218 - +0.5 4,414,223 + +0.4 4,414,223 + +0.4 4,414,224 - +0.1 4,414,266 - -0.5 4,414,319 + -0.6 4,414,320 - +0.1 4,414,431 - -0.5 4,414,431 - -0.1 4,414,555 + Pf6N2E2_3790 0.24 +1.4 4,414,556 - Pf6N2E2_3790 0.24 +0.0 4,414,556 - Pf6N2E2_3790 0.24 -0.2 4,414,558 + Pf6N2E2_3790 0.25 -3.3 4,414,558 + Pf6N2E2_3790 0.25 -2.4 4,414,743 + Pf6N2E2_3790 0.79 +0.9 4,414,743 + Pf6N2E2_3790 0.79 +0.3 4,414,744 - Pf6N2E2_3790 0.79 -2.2 4,414,744 - Pf6N2E2_3790 0.79 +2.4 4,414,744 - Pf6N2E2_3790 0.79 +1.3 4,414,805 + +0.2 4,414,805 + -0.3 4,414,805 + +1.3 4,414,805 + +1.1 4,414,805 + -0.8 4,414,805 + +1.7 4,414,805 + +0.1 4,414,805 + -2.3 4,414,806 - +0.4 4,414,806 - -0.9 4,414,806 - +1.8 4,414,806 - -0.2 4,414,806 - +0.5 4,414,806 - +1.2 4,414,806 - -1.5 4,414,806 - -0.0 4,414,806 - +0.8 4,414,806 - +0.1 4,414,806 - +0.6 4,414,806 - -1.9 4,414,806 - -0.5 4,414,806 - -0.6 4,414,929 + -1.6 4,415,295 - Pf6N2E2_3791 0.11 -1.6 4,415,375 + Pf6N2E2_3791 0.14 -1.4 4,415,375 + Pf6N2E2_3791 0.14 -3.2 4,415,375 + Pf6N2E2_3791 0.14 -1.2 4,415,375 + Pf6N2E2_3791 0.14 -0.6 4,415,375 + Pf6N2E2_3791 0.14 -2.7 4,415,376 - Pf6N2E2_3791 0.14 +1.9 4,415,376 - Pf6N2E2_3791 0.14 -1.6 4,415,412 - Pf6N2E2_3791 0.16 -3.1 4,415,412 - Pf6N2E2_3791 0.16 -1.3 4,415,821 - Pf6N2E2_3791 0.32 -3.5 4,415,933 + Pf6N2E2_3791 0.36 -2.1 4,415,933 + Pf6N2E2_3791 0.36 -2.1 4,416,014 + Pf6N2E2_3791 0.40 -1.1 4,416,014 + Pf6N2E2_3791 0.40 -2.3 4,416,015 - Pf6N2E2_3791 0.40 -1.8 4,416,176 + Pf6N2E2_3791 0.46 -1.9 4,416,422 + Pf6N2E2_3791 0.56 -2.4 4,416,422 + Pf6N2E2_3791 0.56 -0.5 4,416,423 - Pf6N2E2_3791 0.56 -1.9 4,416,423 - Pf6N2E2_3791 0.56 -2.1 4,416,423 - Pf6N2E2_3791 0.56 -1.9 4,416,517 - Pf6N2E2_3791 0.60 -2.9 4,416,554 + Pf6N2E2_3791 0.61 -0.7 4,416,554 + Pf6N2E2_3791 0.61 -1.8 4,416,554 + Pf6N2E2_3791 0.61 -2.6 4,416,554 + Pf6N2E2_3791 0.61 -2.3 4,416,554 + Pf6N2E2_3791 0.61 -1.0 4,416,555 - Pf6N2E2_3791 0.61 -1.9 4,416,560 + Pf6N2E2_3791 0.61 -1.5 4,416,561 - Pf6N2E2_3791 0.61 -1.6 4,416,561 - Pf6N2E2_3791 0.61 -2.3 4,416,635 + Pf6N2E2_3791 0.64 -2.5 4,416,705 + Pf6N2E2_3791 0.67 -0.9 4,416,717 + Pf6N2E2_3791 0.68 -1.4 4,416,718 - Pf6N2E2_3791 0.68 +0.0 4,416,811 + Pf6N2E2_3791 0.71 -2.7 4,416,812 - Pf6N2E2_3791 0.71 -1.3 4,416,900 - Pf6N2E2_3791 0.75 -1.4 4,417,091 + Pf6N2E2_3791 0.82 -0.6 4,417,113 - Pf6N2E2_3791 0.83 -2.2 4,417,160 + Pf6N2E2_3791 0.85 -2.3 4,417,182 + Pf6N2E2_3791 0.86 -0.4 4,417,183 - Pf6N2E2_3791 0.86 -0.1 4,417,547 + +0.8 4,417,547 + -2.6 4,417,547 + -1.5 4,417,548 - +0.3 4,417,548 - -1.0 4,417,578 + -3.0 4,417,578 + -3.8 4,417,578 + -1.2 4,417,578 + -0.1 4,417,578 + -1.0 4,417,578 + +0.0 4,417,578 + -1.5 4,417,578 + +0.8 4,417,579 - -1.2 4,417,579 - -3.0 4,417,579 - -2.7 4,417,579 - -1.4 4,417,579 - -1.3 4,417,579 - -1.3 4,417,579 - -2.1 4,417,579 - -2.7 4,417,581 + -2.5 4,417,581 + -3.6 4,417,581 + -2.2 4,417,581 + -1.3 4,417,582 - -2.8 4,417,582 - -2.5 4,417,582 - -2.3 4,417,603 - -1.6 4,417,603 - -3.0 4,417,917 + Pf6N2E2_3792 0.19 -2.4 4,417,918 - Pf6N2E2_3792 0.19 -2.0 4,418,029 - Pf6N2E2_3792 0.26 -2.3 4,418,029 - Pf6N2E2_3792 0.26 -2.3 4,418,029 - Pf6N2E2_3792 0.26 -1.5 4,418,029 - Pf6N2E2_3792 0.26 -1.0 4,418,344 - Pf6N2E2_3792 0.44 +1.2 4,418,509 - Pf6N2E2_3792 0.53 -2.0 4,418,509 - Pf6N2E2_3792 0.53 -3.2 4,418,509 - Pf6N2E2_3792 0.53 -1.8
Or see this region's nucleotide sequence