Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3606 and Pf6N2E2_3607 overlap by 4 nucleotides Pf6N2E2_3607 and Pf6N2E2_6125 are separated by 226 nucleotides Pf6N2E2_6125 and Pf6N2E2_6126 are separated by 180 nucleotides Pf6N2E2_6126 and Pf6N2E2_3608 are separated by 194 nucleotides
Pf6N2E2_3606 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 4,196,468 to 4,197,226
_3606
Pf6N2E2_3607 - 3'-to-5' exoribonuclease RNase R, at 4,197,223 to 4,199,853
_3607
Pf6N2E2_6125 - tRNA-Leu-CAG, at 4,200,080 to 4,200,163
_6125
Pf6N2E2_6126 - tRNA-Leu-CAG, at 4,200,344 to 4,200,427
_6126
Pf6N2E2_3608 - Methyl-accepting chemotaxis protein, at 4,200,622 to 4,202,484
_3608
Position (kb)
4197
4198
4199
4200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4196.251 kb on - strand at 4196.251 kb on - strand at 4196.323 kb on - strand at 4196.418 kb on + strand at 4196.418 kb on + strand at 4196.419 kb on - strand at 4196.419 kb on - strand at 4196.456 kb on + strand at 4196.456 kb on + strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.590 kb on - strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.843 kb on - strand, within Pf6N2E2_3606 at 4196.902 kb on + strand, within Pf6N2E2_3606 at 4197.005 kb on + strand, within Pf6N2E2_3606 at 4197.006 kb on - strand, within Pf6N2E2_3606 at 4197.166 kb on + strand at 4197.238 kb on - strand at 4197.238 kb on - strand at 4197.295 kb on - strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.361 kb on - strand at 4197.538 kb on - strand, within Pf6N2E2_3607 at 4197.740 kb on + strand, within Pf6N2E2_3607 at 4197.740 kb on + strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.743 kb on + strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.980 kb on + strand, within Pf6N2E2_3607 at 4197.981 kb on - strand, within Pf6N2E2_3607 at 4198.118 kb on + strand, within Pf6N2E2_3607 at 4198.119 kb on - strand, within Pf6N2E2_3607 at 4198.127 kb on + strand, within Pf6N2E2_3607 at 4198.163 kb on + strand, within Pf6N2E2_3607 at 4198.164 kb on - strand, within Pf6N2E2_3607 at 4198.362 kb on - strand, within Pf6N2E2_3607 at 4198.568 kb on + strand, within Pf6N2E2_3607 at 4198.569 kb on - strand, within Pf6N2E2_3607 at 4198.578 kb on - strand, within Pf6N2E2_3607 at 4198.676 kb on + strand, within Pf6N2E2_3607 at 4198.991 kb on + strand, within Pf6N2E2_3607 at 4198.991 kb on + strand, within Pf6N2E2_3607 at 4198.992 kb on - strand, within Pf6N2E2_3607 at 4199.150 kb on + strand, within Pf6N2E2_3607 at 4199.192 kb on + strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.193 kb on - strand, within Pf6N2E2_3607 at 4199.616 kb on - strand at 4199.634 kb on - strand at 4199.736 kb on + strand at 4199.791 kb on - strand at 4199.874 kb on + strand at 4199.874 kb on + strand at 4199.874 kb on + strand at 4199.876 kb on + strand at 4199.895 kb on + strand at 4199.895 kb on + strand at 4199.895 kb on + strand at 4199.895 kb on + strand at 4200.017 kb on + strand at 4200.023 kb on + strand at 4200.038 kb on + strand at 4200.040 kb on + strand at 4200.061 kb on + strand at 4200.061 kb on + strand at 4200.061 kb on + strand at 4200.261 kb on + strand at 4200.265 kb on - strand at 4200.305 kb on - strand at 4200.307 kb on - strand at 4200.307 kb on - strand at 4200.307 kb on - strand at 4200.510 kb on - strand at 4200.510 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,196,251 - -1.3 4,196,251 - -1.3 4,196,323 - -0.0 4,196,418 + +2.0 4,196,418 + +0.7 4,196,419 - -0.6 4,196,419 - -1.6 4,196,456 + +0.0 4,196,456 + -0.6 4,196,457 - +1.7 4,196,457 - +0.3 4,196,457 - -0.9 4,196,457 - +0.9 4,196,590 - Pf6N2E2_3606 0.16 -1.0 4,196,676 + Pf6N2E2_3606 0.27 -0.4 4,196,676 + Pf6N2E2_3606 0.27 -2.2 4,196,676 + Pf6N2E2_3606 0.27 -1.8 4,196,676 + Pf6N2E2_3606 0.27 -1.3 4,196,676 + Pf6N2E2_3606 0.27 -0.3 4,196,676 + Pf6N2E2_3606 0.27 -0.5 4,196,677 - Pf6N2E2_3606 0.28 -2.3 4,196,677 - Pf6N2E2_3606 0.28 -1.0 4,196,677 - Pf6N2E2_3606 0.28 -3.2 4,196,677 - Pf6N2E2_3606 0.28 -1.0 4,196,677 - Pf6N2E2_3606 0.28 -0.3 4,196,677 - Pf6N2E2_3606 0.28 -1.3 4,196,677 - Pf6N2E2_3606 0.28 +0.2 4,196,677 - Pf6N2E2_3606 0.28 -0.3 4,196,677 - Pf6N2E2_3606 0.28 -0.0 4,196,680 + Pf6N2E2_3606 0.28 -1.0 4,196,680 + Pf6N2E2_3606 0.28 +0.9 4,196,680 + Pf6N2E2_3606 0.28 -1.6 4,196,681 - Pf6N2E2_3606 0.28 +1.7 4,196,681 - Pf6N2E2_3606 0.28 +0.2 4,196,681 - Pf6N2E2_3606 0.28 -1.7 4,196,681 - Pf6N2E2_3606 0.28 -1.4 4,196,681 - Pf6N2E2_3606 0.28 -1.0 4,196,843 - Pf6N2E2_3606 0.49 +0.3 4,196,902 + Pf6N2E2_3606 0.57 -1.4 4,197,005 + Pf6N2E2_3606 0.71 -1.4 4,197,006 - Pf6N2E2_3606 0.71 -0.3 4,197,166 + +0.1 4,197,238 - -1.1 4,197,238 - -1.4 4,197,295 - +1.2 4,197,303 + +0.2 4,197,303 + +0.6 4,197,303 + +0.1 4,197,303 + +1.2 4,197,303 + +0.7 4,197,304 - +0.7 4,197,304 - -0.2 4,197,304 - -0.8 4,197,304 - +1.8 4,197,361 - +1.3 4,197,538 - Pf6N2E2_3607 0.12 +0.9 4,197,740 + Pf6N2E2_3607 0.20 -0.5 4,197,740 + Pf6N2E2_3607 0.20 -2.3 4,197,741 - Pf6N2E2_3607 0.20 -1.0 4,197,741 - Pf6N2E2_3607 0.20 -1.9 4,197,741 - Pf6N2E2_3607 0.20 -2.3 4,197,741 - Pf6N2E2_3607 0.20 -0.2 4,197,743 + Pf6N2E2_3607 0.20 -0.4 4,197,744 - Pf6N2E2_3607 0.20 -2.3 4,197,744 - Pf6N2E2_3607 0.20 -2.5 4,197,744 - Pf6N2E2_3607 0.20 -1.0 4,197,785 + Pf6N2E2_3607 0.21 -0.8 4,197,785 + Pf6N2E2_3607 0.21 -1.8 4,197,785 + Pf6N2E2_3607 0.21 -1.2 4,197,785 + Pf6N2E2_3607 0.21 -2.2 4,197,786 - Pf6N2E2_3607 0.21 +1.2 4,197,786 - Pf6N2E2_3607 0.21 -2.7 4,197,786 - Pf6N2E2_3607 0.21 -0.4 4,197,980 + Pf6N2E2_3607 0.29 -1.0 4,197,981 - Pf6N2E2_3607 0.29 -2.0 4,198,118 + Pf6N2E2_3607 0.34 -1.9 4,198,119 - Pf6N2E2_3607 0.34 -0.8 4,198,127 + Pf6N2E2_3607 0.34 -1.3 4,198,163 + Pf6N2E2_3607 0.36 -1.1 4,198,164 - Pf6N2E2_3607 0.36 -1.5 4,198,362 - Pf6N2E2_3607 0.43 -1.0 4,198,568 + Pf6N2E2_3607 0.51 +0.3 4,198,569 - Pf6N2E2_3607 0.51 -1.5 4,198,578 - Pf6N2E2_3607 0.52 -0.8 4,198,676 + Pf6N2E2_3607 0.55 -1.3 4,198,991 + Pf6N2E2_3607 0.67 -1.2 4,198,991 + Pf6N2E2_3607 0.67 -0.7 4,198,992 - Pf6N2E2_3607 0.67 -1.3 4,199,150 + Pf6N2E2_3607 0.73 -0.2 4,199,192 + Pf6N2E2_3607 0.75 -1.8 4,199,193 - Pf6N2E2_3607 0.75 -0.7 4,199,193 - Pf6N2E2_3607 0.75 -1.3 4,199,193 - Pf6N2E2_3607 0.75 -2.4 4,199,193 - Pf6N2E2_3607 0.75 -1.1 4,199,193 - Pf6N2E2_3607 0.75 -1.3 4,199,193 - Pf6N2E2_3607 0.75 -1.8 4,199,616 - -0.7 4,199,634 - -1.1 4,199,736 + -0.1 4,199,791 - -1.5 4,199,874 + -1.2 4,199,874 + -0.4 4,199,874 + -0.1 4,199,876 + -0.1 4,199,895 + -1.1 4,199,895 + -0.8 4,199,895 + -1.0 4,199,895 + -0.7 4,200,017 + -1.0 4,200,023 + -1.0 4,200,038 + -0.2 4,200,040 + -2.3 4,200,061 + -1.1 4,200,061 + -2.1 4,200,061 + -0.2 4,200,261 + +1.6 4,200,265 - +1.2 4,200,305 - +0.8 4,200,307 - -1.8 4,200,307 - +0.2 4,200,307 - -1.0 4,200,510 - +1.4 4,200,510 - +1.0
Or see this region's nucleotide sequence