Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3431 and Pf6N2E2_3432 are separated by 24 nucleotides Pf6N2E2_3432 and Pf6N2E2_3433 are separated by 238 nucleotides Pf6N2E2_3433 and Pf6N2E2_3434 are separated by 362 nucleotides
Pf6N2E2_3431 - PAP2 superfamily protein, at 3,988,873 to 3,989,721
_3431
Pf6N2E2_3432 - Ais protein, putative, at 3,989,746 to 3,990,438
_3432
Pf6N2E2_3433 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,990,677 to 3,992,596
_3433
Pf6N2E2_3434 - FIG00957871: hypothetical protein, at 3,992,959 to 3,995,493
_3434
Position (kb)
3990
3991
3992
3993 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3989.685 kb on + strand at 3989.686 kb on - strand at 3989.686 kb on - strand at 3989.686 kb on - strand at 3989.693 kb on + strand at 3989.731 kb on + strand at 3989.732 kb on - strand at 3989.738 kb on - strand at 3989.747 kb on + strand at 3989.953 kb on - strand, within Pf6N2E2_3432 at 3990.017 kb on + strand, within Pf6N2E2_3432 at 3990.017 kb on + strand, within Pf6N2E2_3432 at 3990.018 kb on - strand, within Pf6N2E2_3432 at 3990.018 kb on - strand, within Pf6N2E2_3432 at 3990.056 kb on + strand, within Pf6N2E2_3432 at 3990.057 kb on - strand, within Pf6N2E2_3432 at 3990.480 kb on + strand at 3990.480 kb on + strand at 3990.561 kb on - strand at 3990.595 kb on + strand at 3990.595 kb on + strand at 3990.595 kb on + strand at 3990.595 kb on + strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.596 kb on - strand at 3990.615 kb on + strand at 3990.616 kb on - strand at 3990.616 kb on - strand at 3990.682 kb on + strand at 3990.682 kb on + strand at 3990.682 kb on + strand at 3990.683 kb on - strand at 3990.683 kb on - strand at 3990.872 kb on + strand, within Pf6N2E2_3433 at 3990.873 kb on - strand, within Pf6N2E2_3433 at 3990.873 kb on - strand, within Pf6N2E2_3433 at 3990.873 kb on - strand, within Pf6N2E2_3433 at 3990.873 kb on - strand, within Pf6N2E2_3433 at 3990.873 kb on - strand, within Pf6N2E2_3433 at 3991.023 kb on - strand, within Pf6N2E2_3433 at 3991.070 kb on - strand, within Pf6N2E2_3433 at 3991.070 kb on - strand, within Pf6N2E2_3433 at 3991.070 kb on - strand, within Pf6N2E2_3433 at 3991.104 kb on + strand, within Pf6N2E2_3433 at 3991.109 kb on + strand, within Pf6N2E2_3433 at 3991.109 kb on + strand, within Pf6N2E2_3433 at 3991.109 kb on + strand, within Pf6N2E2_3433 at 3991.110 kb on - strand, within Pf6N2E2_3433 at 3991.110 kb on - strand, within Pf6N2E2_3433 at 3991.110 kb on - strand, within Pf6N2E2_3433 at 3991.110 kb on - strand, within Pf6N2E2_3433 at 3991.110 kb on - strand, within Pf6N2E2_3433 at 3991.651 kb on + strand, within Pf6N2E2_3433 at 3991.652 kb on - strand, within Pf6N2E2_3433 at 3991.652 kb on - strand, within Pf6N2E2_3433 at 3992.643 kb on + strand at 3992.643 kb on + strand at 3992.644 kb on - strand at 3992.644 kb on - strand at 3992.649 kb on + strand at 3992.757 kb on + strand at 3992.757 kb on + strand at 3992.787 kb on + strand at 3992.788 kb on - strand at 3992.788 kb on - strand at 3992.788 kb on - strand at 3992.808 kb on - strand at 3992.808 kb on - strand at 3992.808 kb on - strand at 3992.808 kb on - strand at 3992.815 kb on - strand at 3992.819 kb on + strand at 3992.820 kb on - strand at 3992.820 kb on - strand at 3992.820 kb on - strand at 3992.820 kb on - strand at 3992.861 kb on + strand at 3992.861 kb on + strand at 3992.909 kb on - strand at 3992.909 kb on - strand at 3993.152 kb on - strand at 3993.152 kb on - strand at 3993.152 kb on - strand at 3993.169 kb on + strand at 3993.170 kb on - strand at 3993.170 kb on - strand at 3993.193 kb on + strand at 3993.194 kb on - strand at 3993.280 kb on + strand, within Pf6N2E2_3434 at 3993.281 kb on - strand, within Pf6N2E2_3434 at 3993.373 kb on + strand, within Pf6N2E2_3434 at 3993.373 kb on + strand, within Pf6N2E2_3434 at 3993.374 kb on - strand, within Pf6N2E2_3434 at 3993.374 kb on - strand, within Pf6N2E2_3434
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,989,685 + +2.0 3,989,686 - +1.1 3,989,686 - -0.5 3,989,686 - +0.7 3,989,693 + -0.0 3,989,731 + +0.8 3,989,732 - -0.4 3,989,738 - -0.2 3,989,747 + +1.9 3,989,953 - Pf6N2E2_3432 0.30 -1.2 3,990,017 + Pf6N2E2_3432 0.39 +1.2 3,990,017 + Pf6N2E2_3432 0.39 -2.3 3,990,018 - Pf6N2E2_3432 0.39 -0.9 3,990,018 - Pf6N2E2_3432 0.39 -0.6 3,990,056 + Pf6N2E2_3432 0.45 +1.7 3,990,057 - Pf6N2E2_3432 0.45 +0.2 3,990,480 + +6.6 3,990,480 + +3.6 3,990,561 - -0.1 3,990,595 + +3.8 3,990,595 + +1.7 3,990,595 + +2.9 3,990,595 + +1.5 3,990,596 - -0.1 3,990,596 - -1.9 3,990,596 - -2.3 3,990,596 - -0.9 3,990,596 - -1.4 3,990,596 - -2.3 3,990,596 - -0.7 3,990,596 - +0.5 3,990,615 + +0.5 3,990,616 - -0.9 3,990,616 - -2.5 3,990,682 + +2.2 3,990,682 + -0.5 3,990,682 + -1.1 3,990,683 - -0.5 3,990,683 - -0.8 3,990,872 + Pf6N2E2_3433 0.10 -1.4 3,990,873 - Pf6N2E2_3433 0.10 +0.1 3,990,873 - Pf6N2E2_3433 0.10 -0.7 3,990,873 - Pf6N2E2_3433 0.10 +0.1 3,990,873 - Pf6N2E2_3433 0.10 -2.3 3,990,873 - Pf6N2E2_3433 0.10 -1.6 3,991,023 - Pf6N2E2_3433 0.18 -1.0 3,991,070 - Pf6N2E2_3433 0.20 -2.0 3,991,070 - Pf6N2E2_3433 0.20 -0.1 3,991,070 - Pf6N2E2_3433 0.20 -1.1 3,991,104 + Pf6N2E2_3433 0.22 -1.2 3,991,109 + Pf6N2E2_3433 0.23 -0.9 3,991,109 + Pf6N2E2_3433 0.23 -3.2 3,991,109 + Pf6N2E2_3433 0.23 -0.9 3,991,110 - Pf6N2E2_3433 0.23 -2.2 3,991,110 - Pf6N2E2_3433 0.23 -1.7 3,991,110 - Pf6N2E2_3433 0.23 -1.1 3,991,110 - Pf6N2E2_3433 0.23 +1.6 3,991,110 - Pf6N2E2_3433 0.23 -1.4 3,991,651 + Pf6N2E2_3433 0.51 -2.8 3,991,652 - Pf6N2E2_3433 0.51 -1.7 3,991,652 - Pf6N2E2_3433 0.51 -0.8 3,992,643 + -3.3 3,992,643 + -1.6 3,992,644 - -0.4 3,992,644 - -0.2 3,992,649 + -0.7 3,992,757 + +1.2 3,992,757 + +0.2 3,992,787 + -0.8 3,992,788 - -1.3 3,992,788 - +0.0 3,992,788 - +0.5 3,992,808 - -0.3 3,992,808 - -0.2 3,992,808 - +0.3 3,992,808 - +1.1 3,992,815 - -0.4 3,992,819 + +1.2 3,992,820 - -0.2 3,992,820 - -1.1 3,992,820 - +0.1 3,992,820 - -0.6 3,992,861 + +0.4 3,992,861 + +0.7 3,992,909 - +0.3 3,992,909 - -1.4 3,993,152 - +0.0 3,993,152 - +0.9 3,993,152 - +1.0 3,993,169 + +1.8 3,993,170 - +0.2 3,993,170 - -1.0 3,993,193 + -0.3 3,993,194 - +0.2 3,993,280 + Pf6N2E2_3434 0.13 +0.1 3,993,281 - Pf6N2E2_3434 0.13 -0.7 3,993,373 + Pf6N2E2_3434 0.16 -0.8 3,993,373 + Pf6N2E2_3434 0.16 -1.2 3,993,374 - Pf6N2E2_3434 0.16 -1.0 3,993,374 - Pf6N2E2_3434 0.16 -1.3
Or see this region's nucleotide sequence