Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_336 and Pf6N2E2_337 are separated by 173 nucleotides Pf6N2E2_337 and Pf6N2E2_338 are separated by 147 nucleotides Pf6N2E2_338 and Pf6N2E2_339 are separated by 6 nucleotides
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Pf6N2E2_338 - Transcriptional regulator, AraC family, at 405,007 to 405,510
_338
Pf6N2E2_339 - Glutathione-regulated potassium-efflux system protein KefC, at 405,517 to 407,343
_339
Position (kb) 405
406 Strain fitness (log2 ratio) -2
-1
0
1 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.116 kb on - strand, within Pf6N2E2_336 at 404.467 kb on + strand at 404.467 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand at 404.669 kb on - strand, within Pf6N2E2_337 at 404.875 kb on + strand at 404.875 kb on + strand at 404.876 kb on - strand at 405.098 kb on + strand, within Pf6N2E2_338 at 405.098 kb on + strand, within Pf6N2E2_338 at 405.732 kb on - strand, within Pf6N2E2_339 at 405.732 kb on - strand, within Pf6N2E2_339 at 406.027 kb on - strand, within Pf6N2E2_339 at 406.064 kb on - strand, within Pf6N2E2_339
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 404,039 - Pf6N2E2_336 0.52 -1.5 404,039 - Pf6N2E2_336 0.52 -0.1 404,039 - Pf6N2E2_336 0.52 +1.1 404,116 - Pf6N2E2_336 0.62 +0.8 404,467 + +0.8 404,467 + -0.5 404,536 + +0.3 404,536 + +0.9 404,536 + +0.5 404,537 - -0.5 404,669 - Pf6N2E2_337 0.30 +0.1 404,875 + -0.7 404,875 + -0.9 404,876 - +0.0 405,098 + Pf6N2E2_338 0.18 -1.1 405,098 + Pf6N2E2_338 0.18 -2.6 405,732 - Pf6N2E2_339 0.12 +0.6 405,732 - Pf6N2E2_339 0.12 -0.5 406,027 - Pf6N2E2_339 0.28 +1.2 406,064 - Pf6N2E2_339 0.30 +0.5
Or see this region's nucleotide sequence