Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3286 and Pf6N2E2_3287 are separated by 172 nucleotides Pf6N2E2_3287 and Pf6N2E2_3288 are separated by 372 nucleotides Pf6N2E2_3288 and Pf6N2E2_3289 are separated by 16 nucleotides
Pf6N2E2_3286 - Rod shape-determining protein MreC, at 3,792,325 to 3,793,446
_3286
Pf6N2E2_3287 - Rod shape-determining protein MreB, at 3,793,619 to 3,794,656
_3287
Pf6N2E2_3288 - Aspartyl-tRNA(Asn) amidotransferase subunit C (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit C (EC 6.3.5.7), at 3,795,029 to 3,795,316
_3288
Pf6N2E2_3289 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 3,795,333 to 3,796,784
_3289
Position (kb)
3793
3794
3795 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3792.671 kb on - strand, within Pf6N2E2_3286 at 3793.514 kb on - strand at 3793.552 kb on - strand at 3793.552 kb on - strand at 3793.552 kb on - strand at 3793.601 kb on - strand at 3793.601 kb on - strand at 3793.601 kb on - strand at 3793.601 kb on - strand at 3793.621 kb on - strand at 3794.801 kb on + strand at 3794.801 kb on + strand at 3794.801 kb on + strand at 3794.904 kb on + strand at 3794.904 kb on + strand at 3794.936 kb on + strand at 3794.936 kb on + strand at 3794.946 kb on + strand at 3794.946 kb on + strand at 3794.954 kb on + strand at 3794.954 kb on + strand at 3794.981 kb on + strand at 3794.981 kb on + strand at 3794.981 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,792,671 - Pf6N2E2_3286 0.31 -0.5 3,793,514 - +1.8 3,793,552 - +0.3 3,793,552 - -0.4 3,793,552 - +0.3 3,793,601 - -2.3 3,793,601 - -0.6 3,793,601 - +0.2 3,793,601 - -0.8 3,793,621 - -1.7 3,794,801 + -1.5 3,794,801 + -3.4 3,794,801 + -2.9 3,794,904 + -1.3 3,794,904 + -1.2 3,794,936 + -1.4 3,794,936 + -2.1 3,794,946 + -1.1 3,794,946 + -1.0 3,794,954 + -1.1 3,794,954 + -0.9 3,794,981 + +0.6 3,794,981 + -2.2 3,794,981 + -1.4
Or see this region's nucleotide sequence