Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3287

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3286 and Pf6N2E2_3287 are separated by 172 nucleotidesPf6N2E2_3287 and Pf6N2E2_3288 are separated by 372 nucleotidesPf6N2E2_3288 and Pf6N2E2_3289 are separated by 16 nucleotides Pf6N2E2_3286 - Rod shape-determining protein MreC, at 3,792,325 to 3,793,446 _3286 Pf6N2E2_3287 - Rod shape-determining protein MreB, at 3,793,619 to 3,794,656 _3287 Pf6N2E2_3288 - Aspartyl-tRNA(Asn) amidotransferase subunit C (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit C (EC 6.3.5.7), at 3,795,029 to 3,795,316 _3288 Pf6N2E2_3289 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 3,795,333 to 3,796,784 _3289 Position (kb) 3793 3794 3795Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3792.671 kb on - strand, within Pf6N2E2_3286at 3793.514 kb on - strandat 3793.552 kb on - strandat 3793.552 kb on - strandat 3793.552 kb on - strandat 3793.601 kb on - strandat 3793.601 kb on - strandat 3793.601 kb on - strandat 3793.601 kb on - strandat 3793.621 kb on - strandat 3794.801 kb on + strandat 3794.801 kb on + strandat 3794.801 kb on + strandat 3794.904 kb on + strandat 3794.904 kb on + strandat 3794.936 kb on + strandat 3794.936 kb on + strandat 3794.946 kb on + strandat 3794.946 kb on + strandat 3794.954 kb on + strandat 3794.954 kb on + strandat 3794.981 kb on + strandat 3794.981 kb on + strandat 3794.981 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,792,671 - Pf6N2E2_3286 0.31 -0.5
3,793,514 - +1.8
3,793,552 - +0.3
3,793,552 - -0.4
3,793,552 - +0.3
3,793,601 - -2.3
3,793,601 - -0.6
3,793,601 - +0.2
3,793,601 - -0.8
3,793,621 - -1.7
3,794,801 + -1.5
3,794,801 + -3.4
3,794,801 + -2.9
3,794,904 + -1.3
3,794,904 + -1.2
3,794,936 + -1.4
3,794,936 + -2.1
3,794,946 + -1.1
3,794,946 + -1.0
3,794,954 + -1.1
3,794,954 + -0.9
3,794,981 + +0.6
3,794,981 + -2.2
3,794,981 + -1.4

Or see this region's nucleotide sequence