Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3117 and Pf6N2E2_3118 are separated by 45 nucleotides Pf6N2E2_3118 and Pf6N2E2_3119 overlap by 4 nucleotides
Pf6N2E2_3117 - diguanylate cyclase (GGDEF domain) with PAS/PAC sensor, at 3,606,029 to 3,607,030
_3117
Pf6N2E2_3118 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,607,076 to 3,608,086
_3118
Pf6N2E2_3119 - Sensor histidine kinase/response regulator, at 3,608,083 to 3,610,347
_3119
Position (kb)
3607
3608
3609 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3606.393 kb on + strand, within Pf6N2E2_3117 at 3606.393 kb on + strand, within Pf6N2E2_3117 at 3606.394 kb on - strand, within Pf6N2E2_3117 at 3606.405 kb on + strand, within Pf6N2E2_3117 at 3606.405 kb on + strand, within Pf6N2E2_3117 at 3606.405 kb on + strand, within Pf6N2E2_3117 at 3606.405 kb on + strand, within Pf6N2E2_3117 at 3606.405 kb on + strand, within Pf6N2E2_3117 at 3606.406 kb on - strand, within Pf6N2E2_3117 at 3606.478 kb on - strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.621 kb on + strand, within Pf6N2E2_3117 at 3606.622 kb on - strand, within Pf6N2E2_3117 at 3606.622 kb on - strand, within Pf6N2E2_3117 at 3606.622 kb on - strand, within Pf6N2E2_3117 at 3606.622 kb on - strand, within Pf6N2E2_3117 at 3606.684 kb on + strand, within Pf6N2E2_3117 at 3606.685 kb on - strand, within Pf6N2E2_3117 at 3606.787 kb on - strand, within Pf6N2E2_3117 at 3607.038 kb on + strand at 3607.038 kb on + strand at 3607.129 kb on + strand at 3607.212 kb on + strand, within Pf6N2E2_3118 at 3607.212 kb on + strand, within Pf6N2E2_3118 at 3607.212 kb on + strand, within Pf6N2E2_3118 at 3607.274 kb on + strand, within Pf6N2E2_3118 at 3607.274 kb on + strand, within Pf6N2E2_3118 at 3607.274 kb on + strand, within Pf6N2E2_3118 at 3607.274 kb on + strand, within Pf6N2E2_3118 at 3608.159 kb on + strand at 3608.159 kb on + strand at 3608.160 kb on - strand at 3608.160 kb on - strand at 3608.160 kb on - strand at 3608.160 kb on - strand at 3608.162 kb on + strand at 3608.163 kb on - strand at 3608.214 kb on - strand at 3608.385 kb on - strand, within Pf6N2E2_3119 at 3608.681 kb on + strand, within Pf6N2E2_3119 at 3608.682 kb on - strand, within Pf6N2E2_3119 at 3608.682 kb on - strand, within Pf6N2E2_3119 at 3608.781 kb on + strand, within Pf6N2E2_3119 at 3608.883 kb on - strand, within Pf6N2E2_3119
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,606,393 + Pf6N2E2_3117 0.36 +0.4 3,606,393 + Pf6N2E2_3117 0.36 +0.4 3,606,394 - Pf6N2E2_3117 0.36 +1.9 3,606,405 + Pf6N2E2_3117 0.38 +0.8 3,606,405 + Pf6N2E2_3117 0.38 +0.8 3,606,405 + Pf6N2E2_3117 0.38 +1.6 3,606,405 + Pf6N2E2_3117 0.38 +0.3 3,606,405 + Pf6N2E2_3117 0.38 -1.8 3,606,406 - Pf6N2E2_3117 0.38 -0.3 3,606,478 - Pf6N2E2_3117 0.45 +1.7 3,606,621 + Pf6N2E2_3117 0.59 -0.2 3,606,621 + Pf6N2E2_3117 0.59 -0.2 3,606,621 + Pf6N2E2_3117 0.59 -0.5 3,606,621 + Pf6N2E2_3117 0.59 +1.6 3,606,621 + Pf6N2E2_3117 0.59 -1.5 3,606,621 + Pf6N2E2_3117 0.59 +0.1 3,606,621 + Pf6N2E2_3117 0.59 -2.5 3,606,621 + Pf6N2E2_3117 0.59 -0.2 3,606,621 + Pf6N2E2_3117 0.59 +1.1 3,606,621 + Pf6N2E2_3117 0.59 +0.2 3,606,622 - Pf6N2E2_3117 0.59 -0.3 3,606,622 - Pf6N2E2_3117 0.59 -1.1 3,606,622 - Pf6N2E2_3117 0.59 +0.1 3,606,622 - Pf6N2E2_3117 0.59 -0.5 3,606,684 + Pf6N2E2_3117 0.65 -0.1 3,606,685 - Pf6N2E2_3117 0.65 +0.6 3,606,787 - Pf6N2E2_3117 0.76 +0.1 3,607,038 + -0.4 3,607,038 + -0.5 3,607,129 + +0.1 3,607,212 + Pf6N2E2_3118 0.13 -2.6 3,607,212 + Pf6N2E2_3118 0.13 +0.3 3,607,212 + Pf6N2E2_3118 0.13 -0.8 3,607,274 + Pf6N2E2_3118 0.20 -3.4 3,607,274 + Pf6N2E2_3118 0.20 -3.1 3,607,274 + Pf6N2E2_3118 0.20 -2.1 3,607,274 + Pf6N2E2_3118 0.20 -1.8 3,608,159 + -0.4 3,608,159 + +0.4 3,608,160 - -2.7 3,608,160 - +1.2 3,608,160 - +1.2 3,608,160 - +0.9 3,608,162 + +0.5 3,608,163 - -0.1 3,608,214 - -0.7 3,608,385 - Pf6N2E2_3119 0.13 -1.3 3,608,681 + Pf6N2E2_3119 0.26 -0.4 3,608,682 - Pf6N2E2_3119 0.26 -0.6 3,608,682 - Pf6N2E2_3119 0.26 -0.4 3,608,781 + Pf6N2E2_3119 0.31 -2.0 3,608,883 - Pf6N2E2_3119 0.35 -1.2
Or see this region's nucleotide sequence