Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3118

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3117 and Pf6N2E2_3118 are separated by 45 nucleotidesPf6N2E2_3118 and Pf6N2E2_3119 overlap by 4 nucleotides Pf6N2E2_3117 - diguanylate cyclase (GGDEF domain) with PAS/PAC sensor, at 3,606,029 to 3,607,030 _3117 Pf6N2E2_3118 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 3,607,076 to 3,608,086 _3118 Pf6N2E2_3119 - Sensor histidine kinase/response regulator, at 3,608,083 to 3,610,347 _3119 Position (kb) 3607 3608 3609Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3606.393 kb on + strand, within Pf6N2E2_3117at 3606.393 kb on + strand, within Pf6N2E2_3117at 3606.394 kb on - strand, within Pf6N2E2_3117at 3606.405 kb on + strand, within Pf6N2E2_3117at 3606.405 kb on + strand, within Pf6N2E2_3117at 3606.405 kb on + strand, within Pf6N2E2_3117at 3606.405 kb on + strand, within Pf6N2E2_3117at 3606.405 kb on + strand, within Pf6N2E2_3117at 3606.406 kb on - strand, within Pf6N2E2_3117at 3606.478 kb on - strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.621 kb on + strand, within Pf6N2E2_3117at 3606.622 kb on - strand, within Pf6N2E2_3117at 3606.622 kb on - strand, within Pf6N2E2_3117at 3606.622 kb on - strand, within Pf6N2E2_3117at 3606.622 kb on - strand, within Pf6N2E2_3117at 3606.684 kb on + strand, within Pf6N2E2_3117at 3606.685 kb on - strand, within Pf6N2E2_3117at 3606.787 kb on - strand, within Pf6N2E2_3117at 3607.038 kb on + strandat 3607.038 kb on + strandat 3607.129 kb on + strandat 3607.212 kb on + strand, within Pf6N2E2_3118at 3607.212 kb on + strand, within Pf6N2E2_3118at 3607.212 kb on + strand, within Pf6N2E2_3118at 3607.274 kb on + strand, within Pf6N2E2_3118at 3607.274 kb on + strand, within Pf6N2E2_3118at 3607.274 kb on + strand, within Pf6N2E2_3118at 3607.274 kb on + strand, within Pf6N2E2_3118at 3608.159 kb on + strandat 3608.159 kb on + strandat 3608.160 kb on - strandat 3608.160 kb on - strandat 3608.160 kb on - strandat 3608.160 kb on - strandat 3608.162 kb on + strandat 3608.163 kb on - strandat 3608.214 kb on - strandat 3608.385 kb on - strand, within Pf6N2E2_3119at 3608.681 kb on + strand, within Pf6N2E2_3119at 3608.682 kb on - strand, within Pf6N2E2_3119at 3608.682 kb on - strand, within Pf6N2E2_3119at 3608.781 kb on + strand, within Pf6N2E2_3119at 3608.883 kb on - strand, within Pf6N2E2_3119

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,606,393 + Pf6N2E2_3117 0.36 +0.4
3,606,393 + Pf6N2E2_3117 0.36 +0.4
3,606,394 - Pf6N2E2_3117 0.36 +1.9
3,606,405 + Pf6N2E2_3117 0.38 +0.8
3,606,405 + Pf6N2E2_3117 0.38 +0.8
3,606,405 + Pf6N2E2_3117 0.38 +1.6
3,606,405 + Pf6N2E2_3117 0.38 +0.3
3,606,405 + Pf6N2E2_3117 0.38 -1.8
3,606,406 - Pf6N2E2_3117 0.38 -0.3
3,606,478 - Pf6N2E2_3117 0.45 +1.7
3,606,621 + Pf6N2E2_3117 0.59 -0.2
3,606,621 + Pf6N2E2_3117 0.59 -0.2
3,606,621 + Pf6N2E2_3117 0.59 -0.5
3,606,621 + Pf6N2E2_3117 0.59 +1.6
3,606,621 + Pf6N2E2_3117 0.59 -1.5
3,606,621 + Pf6N2E2_3117 0.59 +0.1
3,606,621 + Pf6N2E2_3117 0.59 -2.5
3,606,621 + Pf6N2E2_3117 0.59 -0.2
3,606,621 + Pf6N2E2_3117 0.59 +1.1
3,606,621 + Pf6N2E2_3117 0.59 +0.2
3,606,622 - Pf6N2E2_3117 0.59 -0.3
3,606,622 - Pf6N2E2_3117 0.59 -1.1
3,606,622 - Pf6N2E2_3117 0.59 +0.1
3,606,622 - Pf6N2E2_3117 0.59 -0.5
3,606,684 + Pf6N2E2_3117 0.65 -0.1
3,606,685 - Pf6N2E2_3117 0.65 +0.6
3,606,787 - Pf6N2E2_3117 0.76 +0.1
3,607,038 + -0.4
3,607,038 + -0.5
3,607,129 + +0.1
3,607,212 + Pf6N2E2_3118 0.13 -2.6
3,607,212 + Pf6N2E2_3118 0.13 +0.3
3,607,212 + Pf6N2E2_3118 0.13 -0.8
3,607,274 + Pf6N2E2_3118 0.20 -3.4
3,607,274 + Pf6N2E2_3118 0.20 -3.1
3,607,274 + Pf6N2E2_3118 0.20 -2.1
3,607,274 + Pf6N2E2_3118 0.20 -1.8
3,608,159 + -0.4
3,608,159 + +0.4
3,608,160 - -2.7
3,608,160 - +1.2
3,608,160 - +1.2
3,608,160 - +0.9
3,608,162 + +0.5
3,608,163 - -0.1
3,608,214 - -0.7
3,608,385 - Pf6N2E2_3119 0.13 -1.3
3,608,681 + Pf6N2E2_3119 0.26 -0.4
3,608,682 - Pf6N2E2_3119 0.26 -0.6
3,608,682 - Pf6N2E2_3119 0.26 -0.4
3,608,781 + Pf6N2E2_3119 0.31 -2.0
3,608,883 - Pf6N2E2_3119 0.35 -1.2

Or see this region's nucleotide sequence