Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_296 and Pf6N2E2_297 are separated by 308 nucleotides Pf6N2E2_297 and Pf6N2E2_298 are separated by 57 nucleotides Pf6N2E2_298 and Pf6N2E2_299 are separated by 103 nucleotides
Pf6N2E2_296 - Thioredoxin reductase (EC 1.8.1.9), at 364,944 to 365,903
_296
Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617
_297
Pf6N2E2_298 - Outer membrane lipoprotein carrier protein LolA, at 368,675 to 369,298
_298
Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727
_299
Position (kb) 366
367
368
369 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 365.306 kb on + strand, within Pf6N2E2_296 at 365.307 kb on - strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.413 kb on + strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.414 kb on - strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.593 kb on + strand, within Pf6N2E2_296 at 365.594 kb on - strand, within Pf6N2E2_296 at 365.652 kb on - strand, within Pf6N2E2_296 at 365.652 kb on - strand, within Pf6N2E2_296 at 365.712 kb on + strand, within Pf6N2E2_296 at 365.833 kb on + strand at 365.833 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 365.962 kb on + strand at 366.109 kb on + strand at 366.137 kb on + strand at 366.162 kb on + strand at 366.164 kb on + strand at 366.164 kb on + strand at 369.360 kb on - strand at 369.360 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.398 kb on - strand at 369.474 kb on + strand at 369.475 kb on - strand at 369.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 365,306 + Pf6N2E2_296 0.38 +0.6 365,307 - Pf6N2E2_296 0.38 +0.0 365,413 + Pf6N2E2_296 0.49 +0.5 365,413 + Pf6N2E2_296 0.49 +0.6 365,413 + Pf6N2E2_296 0.49 +0.2 365,414 - Pf6N2E2_296 0.49 +0.5 365,414 - Pf6N2E2_296 0.49 -1.6 365,414 - Pf6N2E2_296 0.49 -0.4 365,414 - Pf6N2E2_296 0.49 -1.9 365,414 - Pf6N2E2_296 0.49 +0.2 365,414 - Pf6N2E2_296 0.49 +0.8 365,414 - Pf6N2E2_296 0.49 +0.7 365,414 - Pf6N2E2_296 0.49 +0.6 365,414 - Pf6N2E2_296 0.49 +0.1 365,593 + Pf6N2E2_296 0.68 -1.6 365,593 + Pf6N2E2_296 0.68 +1.3 365,593 + Pf6N2E2_296 0.68 -0.5 365,593 + Pf6N2E2_296 0.68 -0.4 365,593 + Pf6N2E2_296 0.68 -0.1 365,593 + Pf6N2E2_296 0.68 +0.4 365,594 - Pf6N2E2_296 0.68 -1.0 365,652 - Pf6N2E2_296 0.74 -3.1 365,652 - Pf6N2E2_296 0.74 +0.2 365,712 + Pf6N2E2_296 0.80 -1.1 365,833 + -0.1 365,833 + -1.7 365,962 + -2.2 365,962 + +0.3 365,962 + +0.8 365,962 + -0.3 365,962 + +0.3 366,109 + -0.7 366,137 + +1.2 366,162 + +0.9 366,164 + -0.1 366,164 + +0.1 369,360 - +0.2 369,360 - +0.7 369,394 - -0.6 369,394 - -2.3 369,394 - +1.5 369,397 + -2.9 369,397 + +2.3 369,397 + -2.5 369,397 + +1.6 369,398 - +1.8 369,474 + +0.4 369,475 - -2.5 369,475 - +2.7
Or see this region's nucleotide sequence