Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2949 and Pf6N2E2_2950 are separated by 88 nucleotides Pf6N2E2_2950 and Pf6N2E2_2951 are separated by 5 nucleotides Pf6N2E2_2951 and Pf6N2E2_2952 are separated by 290 nucleotides Pf6N2E2_2952 and Pf6N2E2_2953 overlap by 2 nucleotides
Pf6N2E2_2949 - UPF0225 protein YchJ, at 3,440,431 to 3,440,919
_2949
Pf6N2E2_2950 - Conserved secreted protein, at 3,441,008 to 3,441,475
_2950
Pf6N2E2_2951 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,441,481 to 3,441,684
_2951
Pf6N2E2_2952 - Isochorismatase (EC 3.3.2.1), at 3,441,975 to 3,442,532
_2952
Pf6N2E2_2953 - hypothetical protein, at 3,442,531 to 3,442,653
_2953
Position (kb)
3441
3442 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3440.554 kb on + strand, within Pf6N2E2_2949 at 3440.554 kb on + strand, within Pf6N2E2_2949 at 3440.556 kb on + strand, within Pf6N2E2_2949 at 3440.563 kb on + strand, within Pf6N2E2_2949 at 3440.563 kb on + strand, within Pf6N2E2_2949 at 3440.563 kb on + strand, within Pf6N2E2_2949 at 3440.564 kb on - strand, within Pf6N2E2_2949 at 3440.564 kb on - strand, within Pf6N2E2_2949 at 3440.564 kb on - strand, within Pf6N2E2_2949 at 3440.564 kb on - strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.567 kb on + strand, within Pf6N2E2_2949 at 3440.568 kb on - strand, within Pf6N2E2_2949 at 3440.568 kb on - strand, within Pf6N2E2_2949 at 3440.568 kb on - strand, within Pf6N2E2_2949 at 3440.584 kb on + strand, within Pf6N2E2_2949 at 3440.584 kb on + strand, within Pf6N2E2_2949 at 3440.585 kb on - strand, within Pf6N2E2_2949 at 3440.699 kb on + strand, within Pf6N2E2_2949 at 3440.699 kb on + strand, within Pf6N2E2_2949 at 3440.700 kb on - strand, within Pf6N2E2_2949 at 3440.700 kb on - strand, within Pf6N2E2_2949 at 3440.700 kb on - strand, within Pf6N2E2_2949 at 3440.700 kb on - strand, within Pf6N2E2_2949 at 3440.700 kb on - strand, within Pf6N2E2_2949 at 3440.767 kb on - strand, within Pf6N2E2_2949 at 3440.812 kb on + strand, within Pf6N2E2_2949 at 3440.812 kb on + strand, within Pf6N2E2_2949 at 3440.812 kb on + strand, within Pf6N2E2_2949 at 3440.813 kb on - strand, within Pf6N2E2_2949 at 3440.813 kb on - strand, within Pf6N2E2_2949 at 3440.906 kb on - strand at 3440.923 kb on + strand at 3440.924 kb on - strand at 3440.956 kb on - strand at 3441.093 kb on + strand, within Pf6N2E2_2950 at 3441.093 kb on + strand, within Pf6N2E2_2950 at 3441.093 kb on + strand, within Pf6N2E2_2950 at 3441.093 kb on + strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.094 kb on - strand, within Pf6N2E2_2950 at 3441.109 kb on - strand, within Pf6N2E2_2950 at 3441.109 kb on - strand, within Pf6N2E2_2950 at 3441.207 kb on - strand, within Pf6N2E2_2950 at 3441.288 kb on - strand, within Pf6N2E2_2950 at 3441.405 kb on + strand, within Pf6N2E2_2950 at 3441.419 kb on + strand, within Pf6N2E2_2950 at 3441.419 kb on + strand, within Pf6N2E2_2950 at 3441.419 kb on + strand, within Pf6N2E2_2950 at 3441.420 kb on - strand, within Pf6N2E2_2950 at 3441.420 kb on - strand, within Pf6N2E2_2950 at 3441.420 kb on - strand, within Pf6N2E2_2950 at 3441.420 kb on - strand, within Pf6N2E2_2950 at 3441.420 kb on - strand, within Pf6N2E2_2950 at 3441.436 kb on + strand at 3441.436 kb on + strand at 3441.437 kb on - strand at 3441.437 kb on - strand at 3441.437 kb on - strand at 3441.448 kb on - strand at 3441.473 kb on + strand at 3441.714 kb on + strand at 3441.714 kb on + strand at 3441.714 kb on + strand at 3441.974 kb on - strand at 3441.974 kb on - strand at 3442.019 kb on - strand at 3442.019 kb on - strand at 3442.084 kb on + strand, within Pf6N2E2_2952 at 3442.085 kb on - strand, within Pf6N2E2_2952 at 3442.103 kb on + strand, within Pf6N2E2_2952 at 3442.103 kb on + strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.410 kb on + strand, within Pf6N2E2_2952 at 3442.411 kb on - strand, within Pf6N2E2_2952 at 3442.411 kb on - strand, within Pf6N2E2_2952 at 3442.454 kb on + strand, within Pf6N2E2_2952 at 3442.454 kb on + strand, within Pf6N2E2_2952 at 3442.455 kb on - strand, within Pf6N2E2_2952 at 3442.524 kb on + strand at 3442.589 kb on - strand, within Pf6N2E2_2953 at 3442.651 kb on + strand at 3442.651 kb on + strand at 3442.651 kb on + strand at 3442.652 kb on - strand at 3442.652 kb on - strand at 3442.652 kb on - strand at 3442.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,440,554 + Pf6N2E2_2949 0.25 -1.3 3,440,554 + Pf6N2E2_2949 0.25 +0.4 3,440,556 + Pf6N2E2_2949 0.26 +2.1 3,440,563 + Pf6N2E2_2949 0.27 +0.7 3,440,563 + Pf6N2E2_2949 0.27 -0.2 3,440,563 + Pf6N2E2_2949 0.27 +0.2 3,440,564 - Pf6N2E2_2949 0.27 +1.6 3,440,564 - Pf6N2E2_2949 0.27 -2.4 3,440,564 - Pf6N2E2_2949 0.27 -1.5 3,440,564 - Pf6N2E2_2949 0.27 -0.2 3,440,567 + Pf6N2E2_2949 0.28 -1.2 3,440,567 + Pf6N2E2_2949 0.28 +1.1 3,440,567 + Pf6N2E2_2949 0.28 -2.1 3,440,567 + Pf6N2E2_2949 0.28 +1.8 3,440,567 + Pf6N2E2_2949 0.28 -2.0 3,440,567 + Pf6N2E2_2949 0.28 +0.4 3,440,567 + Pf6N2E2_2949 0.28 +2.6 3,440,568 - Pf6N2E2_2949 0.28 +2.3 3,440,568 - Pf6N2E2_2949 0.28 -0.3 3,440,568 - Pf6N2E2_2949 0.28 -0.1 3,440,584 + Pf6N2E2_2949 0.31 -0.3 3,440,584 + Pf6N2E2_2949 0.31 +0.6 3,440,585 - Pf6N2E2_2949 0.31 -0.9 3,440,699 + Pf6N2E2_2949 0.55 -0.2 3,440,699 + Pf6N2E2_2949 0.55 +0.4 3,440,700 - Pf6N2E2_2949 0.55 +0.6 3,440,700 - Pf6N2E2_2949 0.55 +0.1 3,440,700 - Pf6N2E2_2949 0.55 +0.1 3,440,700 - Pf6N2E2_2949 0.55 +1.5 3,440,700 - Pf6N2E2_2949 0.55 +1.8 3,440,767 - Pf6N2E2_2949 0.69 +0.0 3,440,812 + Pf6N2E2_2949 0.78 -2.7 3,440,812 + Pf6N2E2_2949 0.78 +2.0 3,440,812 + Pf6N2E2_2949 0.78 +0.5 3,440,813 - Pf6N2E2_2949 0.78 -1.0 3,440,813 - Pf6N2E2_2949 0.78 +0.1 3,440,906 - -0.5 3,440,923 + +2.9 3,440,924 - +1.7 3,440,956 - -0.5 3,441,093 + Pf6N2E2_2950 0.18 +0.3 3,441,093 + Pf6N2E2_2950 0.18 -2.5 3,441,093 + Pf6N2E2_2950 0.18 +0.6 3,441,093 + Pf6N2E2_2950 0.18 -0.5 3,441,094 - Pf6N2E2_2950 0.18 -1.1 3,441,094 - Pf6N2E2_2950 0.18 -1.6 3,441,094 - Pf6N2E2_2950 0.18 -1.0 3,441,094 - Pf6N2E2_2950 0.18 +0.1 3,441,094 - Pf6N2E2_2950 0.18 +0.5 3,441,094 - Pf6N2E2_2950 0.18 -0.1 3,441,109 - Pf6N2E2_2950 0.22 -0.9 3,441,109 - Pf6N2E2_2950 0.22 -0.3 3,441,207 - Pf6N2E2_2950 0.43 +0.6 3,441,288 - Pf6N2E2_2950 0.60 +0.4 3,441,405 + Pf6N2E2_2950 0.85 +0.2 3,441,419 + Pf6N2E2_2950 0.88 +0.1 3,441,419 + Pf6N2E2_2950 0.88 -2.0 3,441,419 + Pf6N2E2_2950 0.88 +0.2 3,441,420 - Pf6N2E2_2950 0.88 -0.0 3,441,420 - Pf6N2E2_2950 0.88 +0.7 3,441,420 - Pf6N2E2_2950 0.88 -1.0 3,441,420 - Pf6N2E2_2950 0.88 -1.3 3,441,420 - Pf6N2E2_2950 0.88 -2.0 3,441,436 + +0.9 3,441,436 + +0.2 3,441,437 - +1.0 3,441,437 - -1.1 3,441,437 - -1.5 3,441,448 - +1.7 3,441,473 + -2.9 3,441,714 + +1.5 3,441,714 + +0.1 3,441,714 + +0.4 3,441,974 - +0.5 3,441,974 - -0.4 3,442,019 - -0.2 3,442,019 - +0.5 3,442,084 + Pf6N2E2_2952 0.20 +1.1 3,442,085 - Pf6N2E2_2952 0.20 -0.0 3,442,103 + Pf6N2E2_2952 0.23 -1.3 3,442,103 + Pf6N2E2_2952 0.23 +1.0 3,442,104 - Pf6N2E2_2952 0.23 +1.0 3,442,104 - Pf6N2E2_2952 0.23 +0.8 3,442,104 - Pf6N2E2_2952 0.23 -0.0 3,442,410 + Pf6N2E2_2952 0.78 -0.0 3,442,411 - Pf6N2E2_2952 0.78 +1.1 3,442,411 - Pf6N2E2_2952 0.78 -1.3 3,442,454 + Pf6N2E2_2952 0.86 +0.9 3,442,454 + Pf6N2E2_2952 0.86 +0.5 3,442,455 - Pf6N2E2_2952 0.86 +0.5 3,442,524 + +0.2 3,442,589 - Pf6N2E2_2953 0.47 -1.7 3,442,651 + -0.1 3,442,651 + -0.3 3,442,651 + -0.0 3,442,652 - +2.0 3,442,652 - -0.5 3,442,652 - -1.0 3,442,652 - -2.4
Or see this region's nucleotide sequence