Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_26 and Pf6N2E2_27 are separated by 108 nucleotides Pf6N2E2_27 and Pf6N2E2_28 are separated by 306 nucleotides Pf6N2E2_28 and Pf6N2E2_29 are separated by 223 nucleotides
Pf6N2E2_26 - Pirin, at 63,017 to 63,883
_26
Pf6N2E2_27 - Mechanosensitive ion channel, at 63,992 to 65,434
_27
Pf6N2E2_28 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 65,741 to 66,181
_28
Pf6N2E2_29 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis @ FIG006045: Sigma factor, ECF subfamily, at 66,405 to 66,905
_29
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 62.996 kb on - strand at 62.996 kb on - strand at 64.089 kb on - strand at 64.367 kb on + strand, within Pf6N2E2_27 at 64.367 kb on + strand, within Pf6N2E2_27 at 64.367 kb on + strand, within Pf6N2E2_27 at 64.367 kb on + strand, within Pf6N2E2_27 at 64.368 kb on - strand, within Pf6N2E2_27 at 64.368 kb on - strand, within Pf6N2E2_27 at 64.814 kb on + strand, within Pf6N2E2_27 at 64.815 kb on - strand, within Pf6N2E2_27 at 65.279 kb on + strand, within Pf6N2E2_27 at 65.280 kb on - strand, within Pf6N2E2_27 at 65.343 kb on + strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.344 kb on - strand at 65.686 kb on - strand at 65.709 kb on + strand at 65.709 kb on + strand at 65.715 kb on + strand at 65.716 kb on - strand at 65.762 kb on + strand at 65.763 kb on - strand at 66.062 kb on + strand, within Pf6N2E2_28 at 66.063 kb on - strand, within Pf6N2E2_28 at 66.063 kb on - strand, within Pf6N2E2_28 at 66.208 kb on + strand at 66.209 kb on - strand at 66.248 kb on + strand at 66.248 kb on + strand at 66.248 kb on + strand at 66.248 kb on + strand at 66.249 kb on - strand at 66.249 kb on - strand at 66.296 kb on + strand at 66.296 kb on + strand at 66.296 kb on + strand at 66.297 kb on - strand at 66.297 kb on - strand at 66.297 kb on - strand at 66.302 kb on + strand at 66.302 kb on + strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.303 kb on - strand at 66.342 kb on + strand at 66.343 kb on - strand at 66.343 kb on - strand at 66.343 kb on - strand at 66.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 62,996 - -0.6 62,996 - -1.1 64,089 - -0.9 64,367 + Pf6N2E2_27 0.26 +1.7 64,367 + Pf6N2E2_27 0.26 +1.7 64,367 + Pf6N2E2_27 0.26 +0.5 64,367 + Pf6N2E2_27 0.26 +1.1 64,368 - Pf6N2E2_27 0.26 +1.1 64,368 - Pf6N2E2_27 0.26 +0.8 64,814 + Pf6N2E2_27 0.57 -0.7 64,815 - Pf6N2E2_27 0.57 -1.6 65,279 + Pf6N2E2_27 0.89 +0.6 65,280 - Pf6N2E2_27 0.89 +0.4 65,343 + +0.4 65,344 - +0.0 65,344 - -2.9 65,344 - -0.1 65,344 - -0.8 65,344 - -1.6 65,344 - -0.2 65,344 - -0.2 65,686 - -0.1 65,709 + -1.4 65,709 + +1.5 65,715 + -0.6 65,716 - +0.7 65,762 + -0.2 65,763 - -0.4 66,062 + Pf6N2E2_28 0.73 +0.4 66,063 - Pf6N2E2_28 0.73 +0.5 66,063 - Pf6N2E2_28 0.73 +1.4 66,208 + -1.1 66,209 - +1.3 66,248 + +1.0 66,248 + +1.3 66,248 + -1.3 66,248 + -0.1 66,249 - -0.6 66,249 - -2.1 66,296 + -1.8 66,296 + -1.7 66,296 + -3.3 66,297 - +1.3 66,297 - +0.8 66,297 - -0.8 66,302 + +0.5 66,302 + +0.3 66,303 - -0.3 66,303 - -1.0 66,303 - +0.5 66,303 - -0.9 66,303 - -1.4 66,303 - -1.3 66,303 - -1.6 66,342 + +0.5 66,343 - -1.2 66,343 - -2.8 66,343 - -0.7 66,355 + +0.3
Or see this region's nucleotide sequence