Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2632 and Pf6N2E2_2633 are separated by 192 nucleotides Pf6N2E2_2633 and Pf6N2E2_2634 are separated by 162 nucleotides Pf6N2E2_2634 and Pf6N2E2_2635 overlap by 44 nucleotides
Pf6N2E2_2632 - tyrosine aminotransferase (EC 2.6.1.57) (from data) , at 3,093,977 to 3,095,170
_2632
Pf6N2E2_2633 - Nitroreductase family protein, at 3,095,363 to 3,095,926
_2633
Pf6N2E2_2634 - Potassium uptake protein TrkH, at 3,096,089 to 3,097,543
_2634
Pf6N2E2_2635 - Transcriptional regulator, AraC family, at 3,097,500 to 3,098,561
_2635
Position (kb)
3096
3097
3098 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3095.509 kb on - strand, within Pf6N2E2_2633 at 3095.553 kb on + strand, within Pf6N2E2_2633 at 3095.554 kb on - strand, within Pf6N2E2_2633 at 3095.554 kb on - strand, within Pf6N2E2_2633 at 3095.954 kb on + strand at 3095.954 kb on + strand at 3095.954 kb on + strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.955 kb on - strand at 3095.959 kb on + strand at 3095.959 kb on + strand at 3095.959 kb on + strand at 3095.960 kb on - strand at 3095.960 kb on - strand at 3096.050 kb on + strand at 3096.235 kb on + strand, within Pf6N2E2_2634 at 3096.235 kb on + strand, within Pf6N2E2_2634 at 3096.235 kb on + strand, within Pf6N2E2_2634 at 3096.235 kb on + strand, within Pf6N2E2_2634 at 3096.308 kb on + strand, within Pf6N2E2_2634 at 3096.308 kb on + strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.309 kb on - strand, within Pf6N2E2_2634 at 3096.343 kb on + strand, within Pf6N2E2_2634 at 3096.343 kb on + strand, within Pf6N2E2_2634 at 3096.344 kb on - strand, within Pf6N2E2_2634 at 3096.386 kb on + strand, within Pf6N2E2_2634 at 3096.386 kb on + strand, within Pf6N2E2_2634 at 3096.387 kb on - strand, within Pf6N2E2_2634 at 3096.409 kb on + strand, within Pf6N2E2_2634 at 3096.409 kb on + strand, within Pf6N2E2_2634 at 3096.409 kb on + strand, within Pf6N2E2_2634 at 3096.409 kb on + strand, within Pf6N2E2_2634 at 3096.410 kb on - strand, within Pf6N2E2_2634 at 3096.653 kb on + strand, within Pf6N2E2_2634 at 3096.653 kb on + strand, within Pf6N2E2_2634 at 3096.653 kb on + strand, within Pf6N2E2_2634 at 3096.653 kb on + strand, within Pf6N2E2_2634 at 3096.854 kb on + strand, within Pf6N2E2_2634 at 3096.854 kb on + strand, within Pf6N2E2_2634 at 3096.855 kb on - strand, within Pf6N2E2_2634 at 3097.059 kb on - strand, within Pf6N2E2_2634 at 3097.097 kb on + strand, within Pf6N2E2_2634 at 3097.098 kb on - strand, within Pf6N2E2_2634 at 3097.169 kb on + strand, within Pf6N2E2_2634 at 3097.169 kb on + strand, within Pf6N2E2_2634 at 3097.170 kb on - strand, within Pf6N2E2_2634 at 3097.193 kb on - strand, within Pf6N2E2_2634 at 3097.232 kb on + strand, within Pf6N2E2_2634 at 3097.232 kb on + strand, within Pf6N2E2_2634 at 3097.232 kb on + strand, within Pf6N2E2_2634 at 3097.232 kb on + strand, within Pf6N2E2_2634 at 3097.233 kb on - strand, within Pf6N2E2_2634 at 3097.233 kb on - strand, within Pf6N2E2_2634 at 3097.276 kb on + strand, within Pf6N2E2_2634 at 3097.276 kb on + strand, within Pf6N2E2_2634 at 3097.276 kb on + strand, within Pf6N2E2_2634 at 3097.277 kb on - strand, within Pf6N2E2_2634 at 3097.519 kb on + strand at 3097.519 kb on + strand at 3097.651 kb on + strand, within Pf6N2E2_2635 at 3097.651 kb on + strand, within Pf6N2E2_2635 at 3097.651 kb on + strand, within Pf6N2E2_2635 at 3097.652 kb on - strand, within Pf6N2E2_2635 at 3097.690 kb on + strand, within Pf6N2E2_2635 at 3097.845 kb on + strand, within Pf6N2E2_2635 at 3097.845 kb on + strand, within Pf6N2E2_2635 at 3097.846 kb on - strand, within Pf6N2E2_2635 at 3097.846 kb on - strand, within Pf6N2E2_2635 at 3097.846 kb on - strand, within Pf6N2E2_2635 at 3097.894 kb on + strand, within Pf6N2E2_2635 at 3097.894 kb on + strand, within Pf6N2E2_2635 at 3097.895 kb on - strand, within Pf6N2E2_2635 at 3097.990 kb on + strand, within Pf6N2E2_2635 at 3097.991 kb on - strand, within Pf6N2E2_2635 at 3098.345 kb on + strand, within Pf6N2E2_2635 at 3098.345 kb on + strand, within Pf6N2E2_2635 at 3098.346 kb on - strand, within Pf6N2E2_2635
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,095,509 - Pf6N2E2_2633 0.26 +0.2 3,095,553 + Pf6N2E2_2633 0.34 -2.3 3,095,554 - Pf6N2E2_2633 0.34 -1.8 3,095,554 - Pf6N2E2_2633 0.34 +0.3 3,095,954 + -1.1 3,095,954 + +0.0 3,095,954 + +1.4 3,095,955 - +1.0 3,095,955 - -0.2 3,095,955 - -0.2 3,095,955 - -1.6 3,095,959 + +0.9 3,095,959 + -1.2 3,095,959 + -0.6 3,095,960 - +1.7 3,095,960 - +0.1 3,096,050 + +1.0 3,096,235 + Pf6N2E2_2634 0.10 -2.0 3,096,235 + Pf6N2E2_2634 0.10 -1.7 3,096,235 + Pf6N2E2_2634 0.10 +0.6 3,096,235 + Pf6N2E2_2634 0.10 -1.0 3,096,308 + Pf6N2E2_2634 0.15 +0.0 3,096,308 + Pf6N2E2_2634 0.15 -1.8 3,096,309 - Pf6N2E2_2634 0.15 -1.1 3,096,309 - Pf6N2E2_2634 0.15 -0.8 3,096,309 - Pf6N2E2_2634 0.15 -0.3 3,096,309 - Pf6N2E2_2634 0.15 -1.0 3,096,309 - Pf6N2E2_2634 0.15 -2.1 3,096,309 - Pf6N2E2_2634 0.15 -1.9 3,096,309 - Pf6N2E2_2634 0.15 -1.8 3,096,309 - Pf6N2E2_2634 0.15 -1.0 3,096,343 + Pf6N2E2_2634 0.17 -1.5 3,096,343 + Pf6N2E2_2634 0.17 -0.9 3,096,344 - Pf6N2E2_2634 0.18 -1.0 3,096,386 + Pf6N2E2_2634 0.20 -1.2 3,096,386 + Pf6N2E2_2634 0.20 -1.3 3,096,387 - Pf6N2E2_2634 0.20 -0.2 3,096,409 + Pf6N2E2_2634 0.22 -1.8 3,096,409 + Pf6N2E2_2634 0.22 +0.4 3,096,409 + Pf6N2E2_2634 0.22 -0.9 3,096,409 + Pf6N2E2_2634 0.22 -1.2 3,096,410 - Pf6N2E2_2634 0.22 -1.3 3,096,653 + Pf6N2E2_2634 0.39 -1.9 3,096,653 + Pf6N2E2_2634 0.39 -1.5 3,096,653 + Pf6N2E2_2634 0.39 -1.3 3,096,653 + Pf6N2E2_2634 0.39 -1.1 3,096,854 + Pf6N2E2_2634 0.53 -2.2 3,096,854 + Pf6N2E2_2634 0.53 -1.2 3,096,855 - Pf6N2E2_2634 0.53 +0.7 3,097,059 - Pf6N2E2_2634 0.67 +0.7 3,097,097 + Pf6N2E2_2634 0.69 -0.3 3,097,098 - Pf6N2E2_2634 0.69 +0.4 3,097,169 + Pf6N2E2_2634 0.74 -1.1 3,097,169 + Pf6N2E2_2634 0.74 -2.5 3,097,170 - Pf6N2E2_2634 0.74 -1.3 3,097,193 - Pf6N2E2_2634 0.76 +0.4 3,097,232 + Pf6N2E2_2634 0.79 -0.4 3,097,232 + Pf6N2E2_2634 0.79 -1.5 3,097,232 + Pf6N2E2_2634 0.79 -0.6 3,097,232 + Pf6N2E2_2634 0.79 -0.3 3,097,233 - Pf6N2E2_2634 0.79 +0.6 3,097,233 - Pf6N2E2_2634 0.79 -1.8 3,097,276 + Pf6N2E2_2634 0.82 -2.1 3,097,276 + Pf6N2E2_2634 0.82 -0.6 3,097,276 + Pf6N2E2_2634 0.82 -1.1 3,097,277 - Pf6N2E2_2634 0.82 -1.2 3,097,519 + -1.4 3,097,519 + -1.1 3,097,651 + Pf6N2E2_2635 0.14 -1.2 3,097,651 + Pf6N2E2_2635 0.14 -0.5 3,097,651 + Pf6N2E2_2635 0.14 +1.0 3,097,652 - Pf6N2E2_2635 0.14 +1.0 3,097,690 + Pf6N2E2_2635 0.18 +0.3 3,097,845 + Pf6N2E2_2635 0.32 -2.1 3,097,845 + Pf6N2E2_2635 0.32 +1.5 3,097,846 - Pf6N2E2_2635 0.33 +1.9 3,097,846 - Pf6N2E2_2635 0.33 -1.3 3,097,846 - Pf6N2E2_2635 0.33 -2.0 3,097,894 + Pf6N2E2_2635 0.37 +0.4 3,097,894 + Pf6N2E2_2635 0.37 +1.9 3,097,895 - Pf6N2E2_2635 0.37 +0.5 3,097,990 + Pf6N2E2_2635 0.46 -1.5 3,097,991 - Pf6N2E2_2635 0.46 -1.5 3,098,345 + Pf6N2E2_2635 0.80 +0.3 3,098,345 + Pf6N2E2_2635 0.80 -0.1 3,098,346 - Pf6N2E2_2635 0.80 +0.4
Or see this region's nucleotide sequence