Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2624 and Pf6N2E2_2625 are separated by 11 nucleotides Pf6N2E2_2625 and Pf6N2E2_2626 are separated by 9 nucleotides Pf6N2E2_2626 and Pf6N2E2_2627 are separated by 70 nucleotides
Pf6N2E2_2624 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,085,645 to 3,086,949
_2624
Pf6N2E2_2625 - Flagellar P-ring protein FlgI, at 3,086,961 to 3,088,067
_2625
Pf6N2E2_2626 - Flagellar L-ring protein FlgH, at 3,088,077 to 3,088,772
_2626
Pf6N2E2_2627 - Flagellar basal-body rod protein FlgG, at 3,088,843 to 3,089,628
_2627
Position (kb)
3086
3087
3088
3089 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3086.061 kb on - strand, within Pf6N2E2_2624 at 3086.189 kb on + strand, within Pf6N2E2_2624 at 3086.448 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.708 kb on + strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.720 kb on + strand, within Pf6N2E2_2624 at 3086.901 kb on - strand at 3086.901 kb on - strand at 3086.940 kb on + strand at 3086.941 kb on - strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.963 kb on - strand at 3086.963 kb on - strand at 3087.352 kb on + strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625 at 3088.058 kb on + strand at 3088.058 kb on + strand at 3088.058 kb on + strand at 3088.059 kb on - strand at 3088.078 kb on + strand at 3088.079 kb on - strand at 3088.159 kb on + strand, within Pf6N2E2_2626 at 3088.159 kb on + strand, within Pf6N2E2_2626 at 3088.159 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.199 kb on + strand, within Pf6N2E2_2626 at 3088.200 kb on - strand, within Pf6N2E2_2626 at 3088.200 kb on - strand, within Pf6N2E2_2626 at 3088.200 kb on - strand, within Pf6N2E2_2626 at 3088.200 kb on - strand, within Pf6N2E2_2626 at 3088.209 kb on - strand, within Pf6N2E2_2626 at 3088.597 kb on + strand, within Pf6N2E2_2626 at 3088.598 kb on - strand, within Pf6N2E2_2626 at 3088.624 kb on + strand, within Pf6N2E2_2626 at 3088.681 kb on + strand, within Pf6N2E2_2626 at 3088.681 kb on + strand, within Pf6N2E2_2626 at 3088.682 kb on - strand, within Pf6N2E2_2626 at 3088.682 kb on - strand, within Pf6N2E2_2626 at 3088.682 kb on - strand, within Pf6N2E2_2626 at 3088.684 kb on + strand, within Pf6N2E2_2626 at 3088.685 kb on - strand, within Pf6N2E2_2626 at 3088.685 kb on - strand, within Pf6N2E2_2626 at 3088.685 kb on - strand, within Pf6N2E2_2626 at 3088.758 kb on + strand at 3088.758 kb on + strand at 3088.758 kb on + strand at 3088.758 kb on + strand at 3088.759 kb on - strand at 3088.759 kb on - strand at 3088.778 kb on + strand at 3088.778 kb on + strand at 3088.778 kb on + strand at 3088.779 kb on - strand at 3088.818 kb on + strand at 3088.818 kb on + strand at 3088.819 kb on - strand at 3088.819 kb on - strand at 3088.819 kb on - strand at 3088.910 kb on + strand at 3089.050 kb on + strand, within Pf6N2E2_2627
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,086,061 - Pf6N2E2_2624 0.32 -2.9 3,086,189 + Pf6N2E2_2624 0.42 -2.8 3,086,448 + Pf6N2E2_2624 0.62 -2.9 3,086,520 + Pf6N2E2_2624 0.67 -3.0 3,086,520 + Pf6N2E2_2624 0.67 -2.8 3,086,521 - Pf6N2E2_2624 0.67 -2.4 3,086,521 - Pf6N2E2_2624 0.67 -3.1 3,086,521 - Pf6N2E2_2624 0.67 -2.9 3,086,521 - Pf6N2E2_2624 0.67 -1.7 3,086,708 + Pf6N2E2_2624 0.81 -1.1 3,086,709 - Pf6N2E2_2624 0.82 +0.4 3,086,709 - Pf6N2E2_2624 0.82 -3.2 3,086,720 + Pf6N2E2_2624 0.82 -1.9 3,086,901 - -2.5 3,086,901 - -0.9 3,086,940 + -3.1 3,086,941 - -1.7 3,086,962 + -2.1 3,086,962 + -1.9 3,086,962 + -3.3 3,086,963 - -2.9 3,086,963 - -0.2 3,087,352 + Pf6N2E2_2625 0.35 -2.8 3,087,353 - Pf6N2E2_2625 0.35 -2.8 3,087,353 - Pf6N2E2_2625 0.35 -3.4 3,087,353 - Pf6N2E2_2625 0.35 -1.0 3,087,353 - Pf6N2E2_2625 0.35 -3.2 3,087,658 + Pf6N2E2_2625 0.63 -3.0 3,087,658 + Pf6N2E2_2625 0.63 -1.5 3,088,058 + -2.4 3,088,058 + -1.5 3,088,058 + -2.6 3,088,059 - -1.4 3,088,078 + -0.7 3,088,079 - -2.9 3,088,159 + Pf6N2E2_2626 0.12 -3.6 3,088,159 + Pf6N2E2_2626 0.12 -1.6 3,088,159 + Pf6N2E2_2626 0.12 -2.1 3,088,199 + Pf6N2E2_2626 0.18 -2.1 3,088,199 + Pf6N2E2_2626 0.18 -1.5 3,088,199 + Pf6N2E2_2626 0.18 -2.9 3,088,199 + Pf6N2E2_2626 0.18 -3.2 3,088,199 + Pf6N2E2_2626 0.18 -2.4 3,088,199 + Pf6N2E2_2626 0.18 -0.7 3,088,199 + Pf6N2E2_2626 0.18 -2.5 3,088,200 - Pf6N2E2_2626 0.18 -3.0 3,088,200 - Pf6N2E2_2626 0.18 -1.8 3,088,200 - Pf6N2E2_2626 0.18 -2.2 3,088,200 - Pf6N2E2_2626 0.18 -3.0 3,088,209 - Pf6N2E2_2626 0.19 -3.0 3,088,597 + Pf6N2E2_2626 0.75 -3.6 3,088,598 - Pf6N2E2_2626 0.75 -4.1 3,088,624 + Pf6N2E2_2626 0.79 -4.0 3,088,681 + Pf6N2E2_2626 0.87 -3.7 3,088,681 + Pf6N2E2_2626 0.87 -2.0 3,088,682 - Pf6N2E2_2626 0.87 -2.7 3,088,682 - Pf6N2E2_2626 0.87 -3.2 3,088,682 - Pf6N2E2_2626 0.87 -4.8 3,088,684 + Pf6N2E2_2626 0.87 -2.2 3,088,685 - Pf6N2E2_2626 0.87 -3.4 3,088,685 - Pf6N2E2_2626 0.87 -1.3 3,088,685 - Pf6N2E2_2626 0.87 -3.7 3,088,758 + -1.5 3,088,758 + -2.8 3,088,758 + -3.3 3,088,758 + -3.5 3,088,759 - -2.0 3,088,759 - -1.6 3,088,778 + -2.3 3,088,778 + -2.0 3,088,778 + -3.4 3,088,779 - -3.2 3,088,818 + -3.5 3,088,818 + -2.8 3,088,819 - +0.0 3,088,819 - -0.5 3,088,819 - -0.4 3,088,910 + -1.8 3,089,050 + Pf6N2E2_2627 0.26 -2.9
Or see this region's nucleotide sequence