Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2623 and Pf6N2E2_2624 are separated by 10 nucleotides Pf6N2E2_2624 and Pf6N2E2_2625 are separated by 11 nucleotides
Pf6N2E2_2623 - Flagellar hook-associated protein FlgK, at 3,083,568 to 3,085,634
_2623
Pf6N2E2_2624 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 3,085,645 to 3,086,949
_2624
Pf6N2E2_2625 - Flagellar P-ring protein FlgI, at 3,086,961 to 3,088,067
_2625
Position (kb)
3085
3086
3087 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3084.964 kb on + strand, within Pf6N2E2_2623 at 3084.964 kb on + strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3084.965 kb on - strand, within Pf6N2E2_2623 at 3085.429 kb on + strand at 3085.436 kb on + strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.437 kb on - strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.438 kb on + strand at 3085.439 kb on - strand at 3085.537 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.617 kb on + strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.618 kb on - strand at 3085.620 kb on - strand at 3085.646 kb on + strand at 3085.646 kb on + strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.647 kb on - strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.648 kb on + strand at 3085.649 kb on - strand at 3085.649 kb on - strand at 3085.649 kb on - strand at 3085.705 kb on + strand at 3085.705 kb on + strand at 3085.706 kb on - strand at 3085.727 kb on + strand at 3085.728 kb on - strand at 3085.728 kb on - strand at 3085.745 kb on + strand at 3085.863 kb on - strand, within Pf6N2E2_2624 at 3085.872 kb on - strand, within Pf6N2E2_2624 at 3085.872 kb on - strand, within Pf6N2E2_2624 at 3086.061 kb on - strand, within Pf6N2E2_2624 at 3086.189 kb on + strand, within Pf6N2E2_2624 at 3086.448 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.520 kb on + strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.521 kb on - strand, within Pf6N2E2_2624 at 3086.708 kb on + strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.709 kb on - strand, within Pf6N2E2_2624 at 3086.720 kb on + strand, within Pf6N2E2_2624 at 3086.901 kb on - strand at 3086.901 kb on - strand at 3086.940 kb on + strand at 3086.941 kb on - strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.962 kb on + strand at 3086.963 kb on - strand at 3086.963 kb on - strand at 3087.352 kb on + strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.353 kb on - strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625 at 3087.658 kb on + strand, within Pf6N2E2_2625
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,084,964 + Pf6N2E2_2623 0.68 -2.2 3,084,964 + Pf6N2E2_2623 0.68 -0.2 3,084,965 - Pf6N2E2_2623 0.68 -3.1 3,084,965 - Pf6N2E2_2623 0.68 -0.9 3,084,965 - Pf6N2E2_2623 0.68 -1.5 3,085,429 + -2.8 3,085,436 + -2.5 3,085,437 - -1.2 3,085,437 - -3.4 3,085,437 - -3.3 3,085,437 - -2.5 3,085,437 - -1.6 3,085,437 - -1.4 3,085,438 + -3.9 3,085,438 + -3.1 3,085,438 + -3.6 3,085,438 + -2.6 3,085,438 + -2.6 3,085,439 - -3.4 3,085,537 + -0.8 3,085,617 + -2.6 3,085,617 + -3.0 3,085,617 + -1.5 3,085,617 + -1.9 3,085,617 + -3.0 3,085,617 + -2.9 3,085,617 + -2.5 3,085,618 - -2.0 3,085,618 - -2.7 3,085,618 - -1.9 3,085,618 - -0.6 3,085,618 - -1.1 3,085,618 - -3.3 3,085,620 - -2.4 3,085,646 + +0.4 3,085,646 + -0.0 3,085,647 - +0.4 3,085,647 - -0.7 3,085,647 - -2.4 3,085,647 - +1.0 3,085,648 + -2.4 3,085,648 + -2.2 3,085,648 + -3.4 3,085,648 + -3.5 3,085,649 - -1.9 3,085,649 - +1.9 3,085,649 - +0.3 3,085,705 + -2.3 3,085,705 + -2.9 3,085,706 - +0.6 3,085,727 + -1.3 3,085,728 - -1.1 3,085,728 - -1.0 3,085,745 + -3.1 3,085,863 - Pf6N2E2_2624 0.17 -2.2 3,085,872 - Pf6N2E2_2624 0.17 -2.6 3,085,872 - Pf6N2E2_2624 0.17 -2.5 3,086,061 - Pf6N2E2_2624 0.32 -2.9 3,086,189 + Pf6N2E2_2624 0.42 -2.8 3,086,448 + Pf6N2E2_2624 0.62 -2.9 3,086,520 + Pf6N2E2_2624 0.67 -3.0 3,086,520 + Pf6N2E2_2624 0.67 -2.8 3,086,521 - Pf6N2E2_2624 0.67 -2.4 3,086,521 - Pf6N2E2_2624 0.67 -3.1 3,086,521 - Pf6N2E2_2624 0.67 -2.9 3,086,521 - Pf6N2E2_2624 0.67 -1.7 3,086,708 + Pf6N2E2_2624 0.81 -1.1 3,086,709 - Pf6N2E2_2624 0.82 +0.4 3,086,709 - Pf6N2E2_2624 0.82 -3.2 3,086,720 + Pf6N2E2_2624 0.82 -1.9 3,086,901 - -2.5 3,086,901 - -0.9 3,086,940 + -3.1 3,086,941 - -1.7 3,086,962 + -2.1 3,086,962 + -1.9 3,086,962 + -3.3 3,086,963 - -2.9 3,086,963 - -0.2 3,087,352 + Pf6N2E2_2625 0.35 -2.8 3,087,353 - Pf6N2E2_2625 0.35 -2.8 3,087,353 - Pf6N2E2_2625 0.35 -3.4 3,087,353 - Pf6N2E2_2625 0.35 -1.0 3,087,353 - Pf6N2E2_2625 0.35 -3.2 3,087,658 + Pf6N2E2_2625 0.63 -3.0 3,087,658 + Pf6N2E2_2625 0.63 -1.5
Or see this region's nucleotide sequence