Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2595 and Pf6N2E2_2596 are separated by 5 nucleotides Pf6N2E2_2596 and Pf6N2E2_2597 are separated by 155 nucleotides
Pf6N2E2_2595 - Flagellar regulatory protein FleQ, at 3,052,327 to 3,053,721
_2595
Pf6N2E2_2596 - Flagellar sensor histidine kinase FleS, at 3,053,727 to 3,054,890
_2596
Pf6N2E2_2597 - Flagellar regulatory protein FleQ, at 3,055,046 to 3,056,521
_2597
Position (kb)
3053
3054
3055 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3052.862 kb on + strand, within Pf6N2E2_2595 at 3052.862 kb on + strand, within Pf6N2E2_2595 at 3052.862 kb on + strand, within Pf6N2E2_2595 at 3052.863 kb on - strand, within Pf6N2E2_2595 at 3052.863 kb on - strand, within Pf6N2E2_2595 at 3052.863 kb on - strand, within Pf6N2E2_2595 at 3052.863 kb on - strand, within Pf6N2E2_2595 at 3052.990 kb on + strand, within Pf6N2E2_2595 at 3053.077 kb on - strand, within Pf6N2E2_2595 at 3053.128 kb on + strand, within Pf6N2E2_2595 at 3053.128 kb on + strand, within Pf6N2E2_2595 at 3053.129 kb on - strand at 3053.129 kb on - strand, within Pf6N2E2_2595 at 3053.129 kb on - strand, within Pf6N2E2_2595 at 3053.129 kb on - strand, within Pf6N2E2_2595 at 3053.215 kb on - strand, within Pf6N2E2_2595 at 3053.216 kb on + strand at 3053.216 kb on + strand, within Pf6N2E2_2595 at 3053.217 kb on - strand, within Pf6N2E2_2595 at 3053.217 kb on - strand, within Pf6N2E2_2595 at 3053.519 kb on - strand, within Pf6N2E2_2595 at 3053.519 kb on - strand, within Pf6N2E2_2595 at 3053.519 kb on - strand, within Pf6N2E2_2595 at 3053.519 kb on - strand, within Pf6N2E2_2595 at 3053.556 kb on + strand, within Pf6N2E2_2595 at 3053.557 kb on - strand, within Pf6N2E2_2595 at 3053.557 kb on - strand, within Pf6N2E2_2595 at 3053.557 kb on - strand, within Pf6N2E2_2595 at 3053.637 kb on - strand at 3053.704 kb on + strand at 3053.882 kb on + strand, within Pf6N2E2_2596 at 3053.974 kb on + strand, within Pf6N2E2_2596 at 3053.974 kb on + strand, within Pf6N2E2_2596 at 3053.974 kb on + strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3053.975 kb on - strand, within Pf6N2E2_2596 at 3054.120 kb on + strand, within Pf6N2E2_2596 at 3054.120 kb on + strand, within Pf6N2E2_2596 at 3054.120 kb on + strand, within Pf6N2E2_2596 at 3054.120 kb on + strand, within Pf6N2E2_2596 at 3054.121 kb on - strand, within Pf6N2E2_2596 at 3054.121 kb on - strand, within Pf6N2E2_2596 at 3054.121 kb on - strand, within Pf6N2E2_2596 at 3054.207 kb on + strand, within Pf6N2E2_2596 at 3054.208 kb on - strand, within Pf6N2E2_2596 at 3054.208 kb on - strand, within Pf6N2E2_2596 at 3054.319 kb on + strand, within Pf6N2E2_2596 at 3054.319 kb on + strand, within Pf6N2E2_2596 at 3054.319 kb on + strand, within Pf6N2E2_2596 at 3054.320 kb on - strand, within Pf6N2E2_2596 at 3054.320 kb on - strand, within Pf6N2E2_2596 at 3054.369 kb on + strand, within Pf6N2E2_2596 at 3054.450 kb on + strand, within Pf6N2E2_2596 at 3054.450 kb on + strand, within Pf6N2E2_2596 at 3054.451 kb on - strand, within Pf6N2E2_2596 at 3054.802 kb on + strand at 3054.802 kb on + strand at 3054.802 kb on + strand at 3054.803 kb on - strand at 3055.090 kb on - strand at 3055.090 kb on - strand at 3055.347 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.611 kb on + strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597 at 3055.612 kb on - strand, within Pf6N2E2_2597
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,052,862 + Pf6N2E2_2595 0.38 -3.1 3,052,862 + Pf6N2E2_2595 0.38 -0.4 3,052,862 + Pf6N2E2_2595 0.38 -2.1 3,052,863 - Pf6N2E2_2595 0.38 -0.1 3,052,863 - Pf6N2E2_2595 0.38 -0.4 3,052,863 - Pf6N2E2_2595 0.38 -2.5 3,052,863 - Pf6N2E2_2595 0.38 +0.3 3,052,990 + Pf6N2E2_2595 0.48 -1.6 3,053,077 - Pf6N2E2_2595 0.54 -1.0 3,053,128 + Pf6N2E2_2595 0.57 -2.1 3,053,128 + Pf6N2E2_2595 0.57 -0.5 3,053,129 - +1.1 3,053,129 - Pf6N2E2_2595 0.57 -0.2 3,053,129 - Pf6N2E2_2595 0.57 -1.1 3,053,129 - Pf6N2E2_2595 0.57 -1.4 3,053,215 - Pf6N2E2_2595 0.64 -0.9 3,053,216 + +0.9 3,053,216 + Pf6N2E2_2595 0.64 -1.0 3,053,217 - Pf6N2E2_2595 0.64 -2.3 3,053,217 - Pf6N2E2_2595 0.64 -1.2 3,053,519 - Pf6N2E2_2595 0.85 -0.0 3,053,519 - Pf6N2E2_2595 0.85 -1.7 3,053,519 - Pf6N2E2_2595 0.85 -1.7 3,053,519 - Pf6N2E2_2595 0.85 -2.1 3,053,556 + Pf6N2E2_2595 0.88 -1.2 3,053,557 - Pf6N2E2_2595 0.88 -1.3 3,053,557 - Pf6N2E2_2595 0.88 +0.3 3,053,557 - Pf6N2E2_2595 0.88 -2.5 3,053,637 - -2.8 3,053,704 + -0.8 3,053,882 + Pf6N2E2_2596 0.13 +0.1 3,053,974 + Pf6N2E2_2596 0.21 -2.1 3,053,974 + Pf6N2E2_2596 0.21 -2.8 3,053,974 + Pf6N2E2_2596 0.21 -2.1 3,053,975 - Pf6N2E2_2596 0.21 -1.5 3,053,975 - Pf6N2E2_2596 0.21 -2.4 3,053,975 - Pf6N2E2_2596 0.21 -1.0 3,053,975 - Pf6N2E2_2596 0.21 -0.5 3,053,975 - Pf6N2E2_2596 0.21 -2.4 3,053,975 - Pf6N2E2_2596 0.21 +0.1 3,053,975 - Pf6N2E2_2596 0.21 -0.7 3,054,120 + Pf6N2E2_2596 0.34 -1.8 3,054,120 + Pf6N2E2_2596 0.34 -3.2 3,054,120 + Pf6N2E2_2596 0.34 -2.9 3,054,120 + Pf6N2E2_2596 0.34 -1.7 3,054,121 - Pf6N2E2_2596 0.34 -0.2 3,054,121 - Pf6N2E2_2596 0.34 +0.1 3,054,121 - Pf6N2E2_2596 0.34 -1.4 3,054,207 + Pf6N2E2_2596 0.41 -3.6 3,054,208 - Pf6N2E2_2596 0.41 -0.6 3,054,208 - Pf6N2E2_2596 0.41 +0.1 3,054,319 + Pf6N2E2_2596 0.51 -0.8 3,054,319 + Pf6N2E2_2596 0.51 -2.1 3,054,319 + Pf6N2E2_2596 0.51 -2.7 3,054,320 - Pf6N2E2_2596 0.51 -0.9 3,054,320 - Pf6N2E2_2596 0.51 -0.5 3,054,369 + Pf6N2E2_2596 0.55 -1.9 3,054,450 + Pf6N2E2_2596 0.62 -3.1 3,054,450 + Pf6N2E2_2596 0.62 -1.6 3,054,451 - Pf6N2E2_2596 0.62 -2.4 3,054,802 + -1.7 3,054,802 + -3.3 3,054,802 + -4.0 3,054,803 - -0.9 3,055,090 - -3.4 3,055,090 - -4.7 3,055,347 + Pf6N2E2_2597 0.20 -3.6 3,055,611 + Pf6N2E2_2597 0.38 -4.0 3,055,611 + Pf6N2E2_2597 0.38 -3.4 3,055,611 + Pf6N2E2_2597 0.38 -4.0 3,055,611 + Pf6N2E2_2597 0.38 -3.2 3,055,611 + Pf6N2E2_2597 0.38 -3.4 3,055,611 + Pf6N2E2_2597 0.38 -2.9 3,055,612 - Pf6N2E2_2597 0.38 -3.8 3,055,612 - Pf6N2E2_2597 0.38 -3.2 3,055,612 - Pf6N2E2_2597 0.38 -3.1 3,055,612 - Pf6N2E2_2597 0.38 -4.8 3,055,612 - Pf6N2E2_2597 0.38 -4.1 3,055,612 - Pf6N2E2_2597 0.38 -4.5 3,055,612 - Pf6N2E2_2597 0.38 -3.4
Or see this region's nucleotide sequence