Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2592 and Pf6N2E2_2593 overlap by 8 nucleotides Pf6N2E2_2593 and Pf6N2E2_2594 are separated by 15 nucleotides Pf6N2E2_2594 and Pf6N2E2_2595 are separated by 200 nucleotides
Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007
_2592
Pf6N2E2_2593 - Flagellar M-ring protein FliF, at 3,050,000 to 3,051,784
_2593
Pf6N2E2_2594 - Flagellar hook-basal body complex protein FliE, at 3,051,800 to 3,052,126
_2594
Pf6N2E2_2595 - Flagellar regulatory protein FleQ, at 3,052,327 to 3,053,721
_2595
Position (kb)
3049
3050
3051
3052 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3049.207 kb on + strand, within Pf6N2E2_2592 at 3049.760 kb on + strand, within Pf6N2E2_2592 at 3049.760 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3050.094 kb on + strand at 3050.094 kb on + strand at 3050.095 kb on - strand at 3050.287 kb on + strand, within Pf6N2E2_2593 at 3050.303 kb on + strand, within Pf6N2E2_2593 at 3050.303 kb on + strand, within Pf6N2E2_2593 at 3050.340 kb on + strand, within Pf6N2E2_2593 at 3050.341 kb on - strand, within Pf6N2E2_2593 at 3051.025 kb on - strand, within Pf6N2E2_2593 at 3051.025 kb on - strand, within Pf6N2E2_2593 at 3051.195 kb on + strand, within Pf6N2E2_2593 at 3051.195 kb on + strand, within Pf6N2E2_2593 at 3051.421 kb on + strand, within Pf6N2E2_2593 at 3051.513 kb on + strand, within Pf6N2E2_2593 at 3051.514 kb on - strand, within Pf6N2E2_2593 at 3051.514 kb on - strand, within Pf6N2E2_2593 at 3051.514 kb on - strand, within Pf6N2E2_2593 at 3051.514 kb on - strand, within Pf6N2E2_2593 at 3051.737 kb on + strand at 3051.828 kb on + strand at 3051.829 kb on - strand at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.902 kb on + strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.903 kb on - strand, within Pf6N2E2_2594 at 3051.985 kb on + strand, within Pf6N2E2_2594 at 3052.051 kb on + strand, within Pf6N2E2_2594 at 3052.051 kb on + strand, within Pf6N2E2_2594 at 3052.051 kb on + strand, within Pf6N2E2_2594 at 3052.052 kb on - strand, within Pf6N2E2_2594 at 3052.115 kb on - strand at 3052.131 kb on + strand at 3052.131 kb on + strand at 3052.131 kb on + strand at 3052.132 kb on - strand at 3052.132 kb on - strand at 3052.165 kb on + strand at 3052.165 kb on + strand at 3052.165 kb on + strand at 3052.166 kb on - strand at 3052.166 kb on - strand at 3052.184 kb on + strand at 3052.184 kb on + strand at 3052.184 kb on + strand at 3052.187 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.318 kb on + strand at 3052.319 kb on - strand at 3052.319 kb on - strand at 3052.319 kb on - strand at 3052.319 kb on - strand at 3052.319 kb on - strand at 3052.319 kb on - strand at 3052.324 kb on + strand at 3052.325 kb on - strand at 3052.325 kb on - strand at 3052.343 kb on + strand at 3052.343 kb on + strand at 3052.343 kb on + strand at 3052.344 kb on - strand at 3052.344 kb on - strand at 3052.344 kb on - strand at 3052.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,049,207 + Pf6N2E2_2592 0.21 -1.8 3,049,760 + Pf6N2E2_2592 0.76 -2.2 3,049,760 + Pf6N2E2_2592 0.76 -2.1 3,049,846 + Pf6N2E2_2592 0.84 -2.3 3,049,846 + Pf6N2E2_2592 0.84 -2.7 3,049,846 + Pf6N2E2_2592 0.84 -2.3 3,049,847 - Pf6N2E2_2592 0.84 -1.7 3,049,847 - Pf6N2E2_2592 0.84 -3.4 3,049,847 - Pf6N2E2_2592 0.84 -3.1 3,049,847 - Pf6N2E2_2592 0.84 -2.3 3,049,847 - Pf6N2E2_2592 0.84 -3.2 3,050,094 + -3.1 3,050,094 + -1.2 3,050,095 - -2.9 3,050,287 + Pf6N2E2_2593 0.16 -3.1 3,050,303 + Pf6N2E2_2593 0.17 -3.4 3,050,303 + Pf6N2E2_2593 0.17 -3.1 3,050,340 + Pf6N2E2_2593 0.19 -3.6 3,050,341 - Pf6N2E2_2593 0.19 -0.0 3,051,025 - Pf6N2E2_2593 0.57 -2.4 3,051,025 - Pf6N2E2_2593 0.57 -3.2 3,051,195 + Pf6N2E2_2593 0.67 -2.7 3,051,195 + Pf6N2E2_2593 0.67 -3.3 3,051,421 + Pf6N2E2_2593 0.80 +1.2 3,051,513 + Pf6N2E2_2593 0.85 -2.6 3,051,514 - Pf6N2E2_2593 0.85 +0.7 3,051,514 - Pf6N2E2_2593 0.85 -2.9 3,051,514 - Pf6N2E2_2593 0.85 -2.2 3,051,514 - Pf6N2E2_2593 0.85 -2.0 3,051,737 + -0.0 3,051,828 + -0.6 3,051,829 - -2.3 3,051,902 + Pf6N2E2_2594 0.31 -3.6 3,051,902 + Pf6N2E2_2594 0.31 -0.8 3,051,902 + Pf6N2E2_2594 0.31 +0.5 3,051,902 + Pf6N2E2_2594 0.31 +0.4 3,051,902 + Pf6N2E2_2594 0.31 -3.1 3,051,902 + Pf6N2E2_2594 0.31 -1.4 3,051,903 - Pf6N2E2_2594 0.31 -0.3 3,051,903 - Pf6N2E2_2594 0.31 -2.0 3,051,903 - Pf6N2E2_2594 0.31 -1.6 3,051,903 - Pf6N2E2_2594 0.31 -0.0 3,051,903 - Pf6N2E2_2594 0.31 -2.1 3,051,903 - Pf6N2E2_2594 0.31 -2.3 3,051,903 - Pf6N2E2_2594 0.31 -2.9 3,051,903 - Pf6N2E2_2594 0.31 -3.7 3,051,903 - Pf6N2E2_2594 0.31 -2.8 3,051,903 - Pf6N2E2_2594 0.31 -3.1 3,051,903 - Pf6N2E2_2594 0.31 -2.4 3,051,985 + Pf6N2E2_2594 0.57 -1.3 3,052,051 + Pf6N2E2_2594 0.77 -1.8 3,052,051 + Pf6N2E2_2594 0.77 -3.7 3,052,051 + Pf6N2E2_2594 0.77 -3.7 3,052,052 - Pf6N2E2_2594 0.77 -3.8 3,052,115 - -1.1 3,052,131 + -2.1 3,052,131 + -1.9 3,052,131 + -0.7 3,052,132 - -3.6 3,052,132 - -2.2 3,052,165 + -1.9 3,052,165 + -2.3 3,052,165 + -3.9 3,052,166 - +1.1 3,052,166 - +0.2 3,052,184 + -3.1 3,052,184 + -3.7 3,052,184 + -3.6 3,052,187 + -3.4 3,052,318 + -0.6 3,052,318 + -1.6 3,052,318 + -1.4 3,052,318 + -1.3 3,052,318 + -2.9 3,052,318 + -3.1 3,052,318 + -3.2 3,052,318 + -1.1 3,052,318 + -2.0 3,052,318 + -4.5 3,052,319 - +0.2 3,052,319 - -1.3 3,052,319 - -0.1 3,052,319 - +1.4 3,052,319 - -0.3 3,052,319 - -0.5 3,052,324 + -2.7 3,052,325 - -1.8 3,052,325 - -1.8 3,052,343 + -3.4 3,052,343 + -3.0 3,052,343 + -4.4 3,052,344 - +0.2 3,052,344 - -0.6 3,052,344 - -0.9 3,052,344 - -2.9
Or see this region's nucleotide sequence