Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2591

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2590 and Pf6N2E2_2591 overlap by 11 nucleotidesPf6N2E2_2591 and Pf6N2E2_2592 are separated by 7 nucleotides Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145 _2590 Pf6N2E2_2591 - Flagellar assembly protein FliH, at 3,048,135 to 3,048,980 _2591 Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007 _2592 Position (kb) 3048 3049Strain fitness (log2 ratio) -3 -2 -1 0 1at 3047.188 kb on + strandat 3047.190 kb on + strand, within Pf6N2E2_2590at 3047.190 kb on + strand, within Pf6N2E2_2590at 3047.190 kb on + strand, within Pf6N2E2_2590at 3047.190 kb on + strand, within Pf6N2E2_2590at 3047.191 kb on - strand, within Pf6N2E2_2590at 3047.191 kb on - strand, within Pf6N2E2_2590at 3047.191 kb on - strand, within Pf6N2E2_2590at 3047.391 kb on + strand, within Pf6N2E2_2590at 3047.392 kb on - strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.412 kb on + strand, within Pf6N2E2_2590at 3047.413 kb on - strand, within Pf6N2E2_2590at 3047.413 kb on - strand, within Pf6N2E2_2590at 3047.413 kb on - strand, within Pf6N2E2_2590at 3047.967 kb on + strand, within Pf6N2E2_2590at 3047.967 kb on + strand, within Pf6N2E2_2590at 3047.968 kb on - strand, within Pf6N2E2_2590at 3048.103 kb on - strandat 3048.103 kb on - strandat 3048.180 kb on + strandat 3048.181 kb on - strandat 3048.469 kb on + strand, within Pf6N2E2_2591at 3048.469 kb on + strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.980 kb on + strandat 3048.980 kb on + strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.990 kb on - strandat 3048.990 kb on - strandat 3048.990 kb on - strandat 3049.207 kb on + strand, within Pf6N2E2_2592at 3049.760 kb on + strand, within Pf6N2E2_2592at 3049.760 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,047,188 + -0.0
3,047,190 + Pf6N2E2_2590 0.30 -2.0
3,047,190 + Pf6N2E2_2590 0.30 -1.0
3,047,190 + Pf6N2E2_2590 0.30 -3.4
3,047,190 + Pf6N2E2_2590 0.30 -3.4
3,047,191 - Pf6N2E2_2590 0.30 -2.5
3,047,191 - Pf6N2E2_2590 0.30 -2.2
3,047,191 - Pf6N2E2_2590 0.30 -3.4
3,047,391 + Pf6N2E2_2590 0.44 -2.7
3,047,392 - Pf6N2E2_2590 0.45 -2.7
3,047,412 + Pf6N2E2_2590 0.46 -3.6
3,047,412 + Pf6N2E2_2590 0.46 -3.3
3,047,412 + Pf6N2E2_2590 0.46 -1.6
3,047,412 + Pf6N2E2_2590 0.46 -1.4
3,047,412 + Pf6N2E2_2590 0.46 -2.3
3,047,412 + Pf6N2E2_2590 0.46 -3.5
3,047,412 + Pf6N2E2_2590 0.46 -2.6
3,047,413 - Pf6N2E2_2590 0.46 -0.8
3,047,413 - Pf6N2E2_2590 0.46 -3.7
3,047,413 - Pf6N2E2_2590 0.46 -3.2
3,047,967 + Pf6N2E2_2590 0.87 -2.2
3,047,967 + Pf6N2E2_2590 0.87 -1.7
3,047,968 - Pf6N2E2_2590 0.87 -3.3
3,048,103 - -2.8
3,048,103 - -3.1
3,048,180 + -2.0
3,048,181 - -1.9
3,048,469 + Pf6N2E2_2591 0.39 -3.2
3,048,469 + Pf6N2E2_2591 0.39 -3.4
3,048,470 - Pf6N2E2_2591 0.40 -3.1
3,048,470 - Pf6N2E2_2591 0.40 -1.7
3,048,470 - Pf6N2E2_2591 0.40 -1.7
3,048,470 - Pf6N2E2_2591 0.40 -3.1
3,048,470 - Pf6N2E2_2591 0.40 -1.6
3,048,470 - Pf6N2E2_2591 0.40 -0.9
3,048,470 - Pf6N2E2_2591 0.40 -2.0
3,048,980 + -1.8
3,048,980 + -1.4
3,048,981 - -1.0
3,048,981 - -1.9
3,048,981 - -2.3
3,048,981 - -2.1
3,048,989 + -0.1
3,048,989 + -2.4
3,048,989 + -1.6
3,048,989 + -1.2
3,048,990 - -0.4
3,048,990 - +0.7
3,048,990 - -2.5
3,049,207 + Pf6N2E2_2592 0.21 -1.8
3,049,760 + Pf6N2E2_2592 0.76 -2.2
3,049,760 + Pf6N2E2_2592 0.76 -2.1
3,049,846 + Pf6N2E2_2592 0.84 -2.3
3,049,846 + Pf6N2E2_2592 0.84 -2.7
3,049,846 + Pf6N2E2_2592 0.84 -2.3
3,049,847 - Pf6N2E2_2592 0.84 -1.7
3,049,847 - Pf6N2E2_2592 0.84 -3.4
3,049,847 - Pf6N2E2_2592 0.84 -3.1
3,049,847 - Pf6N2E2_2592 0.84 -2.3
3,049,847 - Pf6N2E2_2592 0.84 -3.2

Or see this region's nucleotide sequence