Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2590 and Pf6N2E2_2591 overlap by 11 nucleotides Pf6N2E2_2591 and Pf6N2E2_2592 are separated by 7 nucleotides
Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145
_2590
Pf6N2E2_2591 - Flagellar assembly protein FliH, at 3,048,135 to 3,048,980
_2591
Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007
_2592
Position (kb)
3048
3049 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3047.188 kb on + strand at 3047.190 kb on + strand, within Pf6N2E2_2590 at 3047.190 kb on + strand, within Pf6N2E2_2590 at 3047.190 kb on + strand, within Pf6N2E2_2590 at 3047.190 kb on + strand, within Pf6N2E2_2590 at 3047.191 kb on - strand, within Pf6N2E2_2590 at 3047.191 kb on - strand, within Pf6N2E2_2590 at 3047.191 kb on - strand, within Pf6N2E2_2590 at 3047.391 kb on + strand, within Pf6N2E2_2590 at 3047.392 kb on - strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.412 kb on + strand, within Pf6N2E2_2590 at 3047.413 kb on - strand, within Pf6N2E2_2590 at 3047.413 kb on - strand, within Pf6N2E2_2590 at 3047.413 kb on - strand, within Pf6N2E2_2590 at 3047.967 kb on + strand, within Pf6N2E2_2590 at 3047.967 kb on + strand, within Pf6N2E2_2590 at 3047.968 kb on - strand, within Pf6N2E2_2590 at 3048.103 kb on - strand at 3048.103 kb on - strand at 3048.180 kb on + strand at 3048.181 kb on - strand at 3048.469 kb on + strand, within Pf6N2E2_2591 at 3048.469 kb on + strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.470 kb on - strand, within Pf6N2E2_2591 at 3048.980 kb on + strand at 3048.980 kb on + strand at 3048.981 kb on - strand at 3048.981 kb on - strand at 3048.981 kb on - strand at 3048.981 kb on - strand at 3048.989 kb on + strand at 3048.989 kb on + strand at 3048.989 kb on + strand at 3048.989 kb on + strand at 3048.990 kb on - strand at 3048.990 kb on - strand at 3048.990 kb on - strand at 3049.207 kb on + strand, within Pf6N2E2_2592 at 3049.760 kb on + strand, within Pf6N2E2_2592 at 3049.760 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.846 kb on + strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592 at 3049.847 kb on - strand, within Pf6N2E2_2592
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,047,188 + -0.0 3,047,190 + Pf6N2E2_2590 0.30 -2.0 3,047,190 + Pf6N2E2_2590 0.30 -1.0 3,047,190 + Pf6N2E2_2590 0.30 -3.4 3,047,190 + Pf6N2E2_2590 0.30 -3.4 3,047,191 - Pf6N2E2_2590 0.30 -2.5 3,047,191 - Pf6N2E2_2590 0.30 -2.2 3,047,191 - Pf6N2E2_2590 0.30 -3.4 3,047,391 + Pf6N2E2_2590 0.44 -2.7 3,047,392 - Pf6N2E2_2590 0.45 -2.7 3,047,412 + Pf6N2E2_2590 0.46 -3.6 3,047,412 + Pf6N2E2_2590 0.46 -3.3 3,047,412 + Pf6N2E2_2590 0.46 -1.6 3,047,412 + Pf6N2E2_2590 0.46 -1.4 3,047,412 + Pf6N2E2_2590 0.46 -2.3 3,047,412 + Pf6N2E2_2590 0.46 -3.5 3,047,412 + Pf6N2E2_2590 0.46 -2.6 3,047,413 - Pf6N2E2_2590 0.46 -0.8 3,047,413 - Pf6N2E2_2590 0.46 -3.7 3,047,413 - Pf6N2E2_2590 0.46 -3.2 3,047,967 + Pf6N2E2_2590 0.87 -2.2 3,047,967 + Pf6N2E2_2590 0.87 -1.7 3,047,968 - Pf6N2E2_2590 0.87 -3.3 3,048,103 - -2.8 3,048,103 - -3.1 3,048,180 + -2.0 3,048,181 - -1.9 3,048,469 + Pf6N2E2_2591 0.39 -3.2 3,048,469 + Pf6N2E2_2591 0.39 -3.4 3,048,470 - Pf6N2E2_2591 0.40 -3.1 3,048,470 - Pf6N2E2_2591 0.40 -1.7 3,048,470 - Pf6N2E2_2591 0.40 -1.7 3,048,470 - Pf6N2E2_2591 0.40 -3.1 3,048,470 - Pf6N2E2_2591 0.40 -1.6 3,048,470 - Pf6N2E2_2591 0.40 -0.9 3,048,470 - Pf6N2E2_2591 0.40 -2.0 3,048,980 + -1.8 3,048,980 + -1.4 3,048,981 - -1.0 3,048,981 - -1.9 3,048,981 - -2.3 3,048,981 - -2.1 3,048,989 + -0.1 3,048,989 + -2.4 3,048,989 + -1.6 3,048,989 + -1.2 3,048,990 - -0.4 3,048,990 - +0.7 3,048,990 - -2.5 3,049,207 + Pf6N2E2_2592 0.21 -1.8 3,049,760 + Pf6N2E2_2592 0.76 -2.2 3,049,760 + Pf6N2E2_2592 0.76 -2.1 3,049,846 + Pf6N2E2_2592 0.84 -2.3 3,049,846 + Pf6N2E2_2592 0.84 -2.7 3,049,846 + Pf6N2E2_2592 0.84 -2.3 3,049,847 - Pf6N2E2_2592 0.84 -1.7 3,049,847 - Pf6N2E2_2592 0.84 -3.4 3,049,847 - Pf6N2E2_2592 0.84 -3.1 3,049,847 - Pf6N2E2_2592 0.84 -2.3 3,049,847 - Pf6N2E2_2592 0.84 -3.2
Or see this region's nucleotide sequence