Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides Pf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotides Pf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides Pf6N2E2_2583 and Pf6N2E2_2584 are separated by 9 nucleotides Pf6N2E2_2584 and Pf6N2E2_2585 are separated by 232 nucleotides
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127
_2581
Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586
_2582
Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627
_2583
Pf6N2E2_2584 - Flagellar biosynthesis protein FliL, at 3,041,637 to 3,042,140
_2584
Pf6N2E2_2585 - Flagellar hook-length control protein FliK, at 3,042,373 to 3,043,746
_2585
Position (kb)
3040
3041
3042 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3039.692 kb on - strand at 3039.692 kb on - strand at 3039.855 kb on + strand, within Pf6N2E2_2581 at 3040.242 kb on + strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.617 kb on - strand at 3040.617 kb on - strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.972 kb on + strand, within Pf6N2E2_2583 at 3040.973 kb on - strand, within Pf6N2E2_2583 at 3041.095 kb on + strand, within Pf6N2E2_2583 at 3041.096 kb on - strand, within Pf6N2E2_2583 at 3041.338 kb on + strand, within Pf6N2E2_2583 at 3041.339 kb on - strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.515 kb on + strand, within Pf6N2E2_2583 at 3041.639 kb on - strand at 3041.639 kb on - strand at 3041.647 kb on - strand at 3041.870 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.114 kb on + strand at 3042.114 kb on + strand at 3042.146 kb on + strand at 3042.147 kb on - strand at 3042.157 kb on + strand at 3042.201 kb on + strand at 3042.304 kb on + strand at 3042.305 kb on - strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.381 kb on - strand at 3042.381 kb on - strand at 3042.383 kb on + strand at 3042.384 kb on - strand at 3042.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,039,692 - -2.7 3,039,692 - -2.5 3,039,855 + Pf6N2E2_2581 0.38 -1.3 3,040,242 + Pf6N2E2_2582 0.25 -2.6 3,040,243 - Pf6N2E2_2582 0.25 -2.4 3,040,243 - Pf6N2E2_2582 0.25 -3.0 3,040,243 - Pf6N2E2_2582 0.25 -2.2 3,040,617 - -1.3 3,040,617 - +0.4 3,040,641 + -2.1 3,040,641 + -3.4 3,040,641 + -2.5 3,040,972 + Pf6N2E2_2583 0.32 -1.0 3,040,973 - Pf6N2E2_2583 0.32 -3.8 3,041,095 + Pf6N2E2_2583 0.45 -0.7 3,041,096 - Pf6N2E2_2583 0.45 -2.4 3,041,338 + Pf6N2E2_2583 0.70 -3.6 3,041,339 - Pf6N2E2_2583 0.70 -2.3 3,041,353 + Pf6N2E2_2583 0.72 -4.2 3,041,353 + Pf6N2E2_2583 0.72 -3.0 3,041,354 - Pf6N2E2_2583 0.72 -2.6 3,041,354 - Pf6N2E2_2583 0.72 -3.4 3,041,354 - Pf6N2E2_2583 0.72 -2.8 3,041,354 - Pf6N2E2_2583 0.72 -4.4 3,041,354 - Pf6N2E2_2583 0.72 -2.5 3,041,515 + Pf6N2E2_2583 0.88 -1.1 3,041,639 - +1.2 3,041,639 - -0.7 3,041,647 - -0.1 3,041,870 + Pf6N2E2_2584 0.46 -1.7 3,041,887 + Pf6N2E2_2584 0.50 -3.3 3,041,887 + Pf6N2E2_2584 0.50 -0.1 3,041,887 + Pf6N2E2_2584 0.50 -1.1 3,041,887 + Pf6N2E2_2584 0.50 -2.7 3,041,888 - Pf6N2E2_2584 0.50 -1.2 3,041,888 - Pf6N2E2_2584 0.50 -0.3 3,041,888 - Pf6N2E2_2584 0.50 +0.6 3,041,888 - Pf6N2E2_2584 0.50 +1.2 3,041,911 + Pf6N2E2_2584 0.54 -0.7 3,041,911 + Pf6N2E2_2584 0.54 -3.2 3,041,911 + Pf6N2E2_2584 0.54 -3.0 3,041,912 - Pf6N2E2_2584 0.55 -0.7 3,041,912 - Pf6N2E2_2584 0.55 +0.9 3,041,912 - Pf6N2E2_2584 0.55 +0.0 3,041,912 - Pf6N2E2_2584 0.55 +0.6 3,041,912 - Pf6N2E2_2584 0.55 +2.0 3,041,912 - Pf6N2E2_2584 0.55 +0.6 3,042,019 + Pf6N2E2_2584 0.76 -3.7 3,042,019 + Pf6N2E2_2584 0.76 -3.0 3,042,040 - Pf6N2E2_2584 0.80 +1.9 3,042,040 - Pf6N2E2_2584 0.80 +0.7 3,042,040 - Pf6N2E2_2584 0.80 -0.1 3,042,114 + -3.0 3,042,114 + -1.2 3,042,146 + -3.0 3,042,147 - -1.0 3,042,157 + -3.2 3,042,201 + -1.7 3,042,304 + -0.5 3,042,305 - -0.1 3,042,315 + +0.3 3,042,315 + +0.7 3,042,315 + -2.2 3,042,315 + +0.0 3,042,380 + -3.3 3,042,380 + -1.4 3,042,380 + -2.5 3,042,381 - -3.4 3,042,381 - -2.3 3,042,383 + -2.2 3,042,384 - -2.3 3,042,417 + -0.1
Or see this region's nucleotide sequence