Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2583

Experiment: inner cut, LB soft agar motility assay

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotidesPf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotidesPf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotidesPf6N2E2_2583 and Pf6N2E2_2584 are separated by 9 nucleotidesPf6N2E2_2584 and Pf6N2E2_2585 are separated by 232 nucleotides Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690 _2580 Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127 _2581 Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586 _2582 Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627 _2583 Pf6N2E2_2584 - Flagellar biosynthesis protein FliL, at 3,041,637 to 3,042,140 _2584 Pf6N2E2_2585 - Flagellar hook-length control protein FliK, at 3,042,373 to 3,043,746 _2585 Position (kb) 3040 3041 3042Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3039.692 kb on - strandat 3039.692 kb on - strandat 3039.855 kb on + strand, within Pf6N2E2_2581at 3040.242 kb on + strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.617 kb on - strandat 3040.617 kb on - strandat 3040.641 kb on + strandat 3040.641 kb on + strandat 3040.641 kb on + strandat 3040.972 kb on + strand, within Pf6N2E2_2583at 3040.973 kb on - strand, within Pf6N2E2_2583at 3041.095 kb on + strand, within Pf6N2E2_2583at 3041.096 kb on - strand, within Pf6N2E2_2583at 3041.338 kb on + strand, within Pf6N2E2_2583at 3041.339 kb on - strand, within Pf6N2E2_2583at 3041.353 kb on + strand, within Pf6N2E2_2583at 3041.353 kb on + strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.515 kb on + strand, within Pf6N2E2_2583at 3041.639 kb on - strandat 3041.639 kb on - strandat 3041.647 kb on - strandat 3041.870 kb on + strand, within Pf6N2E2_2584at 3041.887 kb on + strand, within Pf6N2E2_2584at 3041.887 kb on + strand, within Pf6N2E2_2584at 3041.887 kb on + strand, within Pf6N2E2_2584at 3041.887 kb on + strand, within Pf6N2E2_2584at 3041.888 kb on - strand, within Pf6N2E2_2584at 3041.888 kb on - strand, within Pf6N2E2_2584at 3041.888 kb on - strand, within Pf6N2E2_2584at 3041.888 kb on - strand, within Pf6N2E2_2584at 3041.911 kb on + strand, within Pf6N2E2_2584at 3041.911 kb on + strand, within Pf6N2E2_2584at 3041.911 kb on + strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3041.912 kb on - strand, within Pf6N2E2_2584at 3042.019 kb on + strand, within Pf6N2E2_2584at 3042.019 kb on + strand, within Pf6N2E2_2584at 3042.040 kb on - strand, within Pf6N2E2_2584at 3042.040 kb on - strand, within Pf6N2E2_2584at 3042.040 kb on - strand, within Pf6N2E2_2584at 3042.114 kb on + strandat 3042.114 kb on + strandat 3042.146 kb on + strandat 3042.147 kb on - strandat 3042.157 kb on + strandat 3042.201 kb on + strandat 3042.304 kb on + strandat 3042.305 kb on - strandat 3042.315 kb on + strandat 3042.315 kb on + strandat 3042.315 kb on + strandat 3042.315 kb on + strandat 3042.380 kb on + strandat 3042.380 kb on + strandat 3042.380 kb on + strandat 3042.381 kb on - strandat 3042.381 kb on - strandat 3042.383 kb on + strandat 3042.384 kb on - strandat 3042.417 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
remove
3,039,692 - -2.7
3,039,692 - -2.5
3,039,855 + Pf6N2E2_2581 0.38 -1.3
3,040,242 + Pf6N2E2_2582 0.25 -2.6
3,040,243 - Pf6N2E2_2582 0.25 -2.4
3,040,243 - Pf6N2E2_2582 0.25 -3.0
3,040,243 - Pf6N2E2_2582 0.25 -2.2
3,040,617 - -1.3
3,040,617 - +0.4
3,040,641 + -2.1
3,040,641 + -3.4
3,040,641 + -2.5
3,040,972 + Pf6N2E2_2583 0.32 -1.0
3,040,973 - Pf6N2E2_2583 0.32 -3.8
3,041,095 + Pf6N2E2_2583 0.45 -0.7
3,041,096 - Pf6N2E2_2583 0.45 -2.4
3,041,338 + Pf6N2E2_2583 0.70 -3.6
3,041,339 - Pf6N2E2_2583 0.70 -2.3
3,041,353 + Pf6N2E2_2583 0.72 -4.2
3,041,353 + Pf6N2E2_2583 0.72 -3.0
3,041,354 - Pf6N2E2_2583 0.72 -2.6
3,041,354 - Pf6N2E2_2583 0.72 -3.4
3,041,354 - Pf6N2E2_2583 0.72 -2.8
3,041,354 - Pf6N2E2_2583 0.72 -4.4
3,041,354 - Pf6N2E2_2583 0.72 -2.5
3,041,515 + Pf6N2E2_2583 0.88 -1.1
3,041,639 - +1.2
3,041,639 - -0.7
3,041,647 - -0.1
3,041,870 + Pf6N2E2_2584 0.46 -1.7
3,041,887 + Pf6N2E2_2584 0.50 -3.3
3,041,887 + Pf6N2E2_2584 0.50 -0.1
3,041,887 + Pf6N2E2_2584 0.50 -1.1
3,041,887 + Pf6N2E2_2584 0.50 -2.7
3,041,888 - Pf6N2E2_2584 0.50 -1.2
3,041,888 - Pf6N2E2_2584 0.50 -0.3
3,041,888 - Pf6N2E2_2584 0.50 +0.6
3,041,888 - Pf6N2E2_2584 0.50 +1.2
3,041,911 + Pf6N2E2_2584 0.54 -0.7
3,041,911 + Pf6N2E2_2584 0.54 -3.2
3,041,911 + Pf6N2E2_2584 0.54 -3.0
3,041,912 - Pf6N2E2_2584 0.55 -0.7
3,041,912 - Pf6N2E2_2584 0.55 +0.9
3,041,912 - Pf6N2E2_2584 0.55 +0.0
3,041,912 - Pf6N2E2_2584 0.55 +0.6
3,041,912 - Pf6N2E2_2584 0.55 +2.0
3,041,912 - Pf6N2E2_2584 0.55 +0.6
3,042,019 + Pf6N2E2_2584 0.76 -3.7
3,042,019 + Pf6N2E2_2584 0.76 -3.0
3,042,040 - Pf6N2E2_2584 0.80 +1.9
3,042,040 - Pf6N2E2_2584 0.80 +0.7
3,042,040 - Pf6N2E2_2584 0.80 -0.1
3,042,114 + -3.0
3,042,114 + -1.2
3,042,146 + -3.0
3,042,147 - -1.0
3,042,157 + -3.2
3,042,201 + -1.7
3,042,304 + -0.5
3,042,305 - -0.1
3,042,315 + +0.3
3,042,315 + +0.7
3,042,315 + -2.2
3,042,315 + +0.0
3,042,380 + -3.3
3,042,380 + -1.4
3,042,380 + -2.5
3,042,381 - -3.4
3,042,381 - -2.3
3,042,383 + -2.2
3,042,384 - -2.3
3,042,417 + -0.1

Or see this region's nucleotide sequence