Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2580

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2578 and Pf6N2E2_2579 are separated by 4 nucleotidesPf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotidesPf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotidesPf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotidesPf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides Pf6N2E2_2578 - Flagellar biosynthesis protein FliR, at 3,037,861 to 3,038,643 _2578 Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917 _2579 Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690 _2580 Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127 _2581 Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586 _2582 Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627 _2583 Position (kb) 3038 3039 3040Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3038.077 kb on + strand, within Pf6N2E2_2578at 3038.286 kb on + strand, within Pf6N2E2_2578at 3038.504 kb on + strand, within Pf6N2E2_2578at 3038.607 kb on + strandat 3038.669 kb on - strandat 3038.669 kb on - strandat 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.709 kb on - strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.862 kb on - strand, within Pf6N2E2_2579at 3038.903 kb on + strandat 3038.903 kb on + strandat 3038.904 kb on - strandat 3038.917 kb on + strandat 3038.942 kb on + strandat 3038.943 kb on - strandat 3039.207 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.692 kb on - strandat 3039.692 kb on - strandat 3039.855 kb on + strand, within Pf6N2E2_2581at 3040.242 kb on + strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.617 kb on - strandat 3040.617 kb on - strandat 3040.641 kb on + strandat 3040.641 kb on + strandat 3040.641 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,038,077 + Pf6N2E2_2578 0.28 -2.1
3,038,286 + Pf6N2E2_2578 0.54 -4.1
3,038,504 + Pf6N2E2_2578 0.82 -3.0
3,038,607 + -2.5
3,038,669 - -3.1
3,038,669 - -3.3
3,038,677 - Pf6N2E2_2579 0.11 -2.1
3,038,677 - Pf6N2E2_2579 0.11 -1.2
3,038,691 + Pf6N2E2_2579 0.16 +0.2
3,038,691 + Pf6N2E2_2579 0.16 -1.6
3,038,691 + Pf6N2E2_2579 0.16 -1.7
3,038,691 + Pf6N2E2_2579 0.16 -2.4
3,038,692 - Pf6N2E2_2579 0.16 -2.1
3,038,692 - Pf6N2E2_2579 0.16 -2.9
3,038,692 - Pf6N2E2_2579 0.16 -2.9
3,038,692 - Pf6N2E2_2579 0.16 -3.8
3,038,709 - Pf6N2E2_2579 0.23 -3.0
3,038,861 + Pf6N2E2_2579 0.79 -2.8
3,038,861 + Pf6N2E2_2579 0.79 -0.7
3,038,862 - Pf6N2E2_2579 0.79 -2.7
3,038,903 + -0.7
3,038,903 + -2.5
3,038,904 - -1.2
3,038,917 + -2.0
3,038,942 + -0.8
3,038,943 - +1.3
3,039,207 + Pf6N2E2_2580 0.35 -2.8
3,039,335 + Pf6N2E2_2580 0.53 -1.9
3,039,335 + Pf6N2E2_2580 0.53 -2.0
3,039,336 - Pf6N2E2_2580 0.53 -1.2
3,039,336 - Pf6N2E2_2580 0.53 -1.5
3,039,569 + Pf6N2E2_2580 0.84 -2.8
3,039,569 + Pf6N2E2_2580 0.84 -2.0
3,039,692 - -2.7
3,039,692 - -2.5
3,039,855 + Pf6N2E2_2581 0.38 -1.3
3,040,242 + Pf6N2E2_2582 0.25 -2.6
3,040,243 - Pf6N2E2_2582 0.25 -2.4
3,040,243 - Pf6N2E2_2582 0.25 -3.0
3,040,243 - Pf6N2E2_2582 0.25 -2.2
3,040,617 - -1.3
3,040,617 - +0.4
3,040,641 + -2.1
3,040,641 + -3.4
3,040,641 + -2.5

Or see this region's nucleotide sequence