Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2578

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2577 and Pf6N2E2_2578 are separated by 3 nucleotidesPf6N2E2_2578 and Pf6N2E2_2579 are separated by 4 nucleotidesPf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotides Pf6N2E2_2577 - Flagellar biosynthesis protein FlhB, at 3,036,721 to 3,037,857 _2577 Pf6N2E2_2578 - Flagellar biosynthesis protein FliR, at 3,037,861 to 3,038,643 _2578 Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917 _2579 Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690 _2580 Position (kb) 3037 3038 3039Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3036.884 kb on + strand, within Pf6N2E2_2577at 3036.888 kb on - strand, within Pf6N2E2_2577at 3037.019 kb on + strand, within Pf6N2E2_2577at 3037.020 kb on - strand, within Pf6N2E2_2577at 3037.020 kb on - strand, within Pf6N2E2_2577at 3037.037 kb on + strand, within Pf6N2E2_2577at 3037.175 kb on - strand, within Pf6N2E2_2577at 3037.214 kb on + strand, within Pf6N2E2_2577at 3037.215 kb on - strand, within Pf6N2E2_2577at 3037.215 kb on - strand, within Pf6N2E2_2577at 3037.300 kb on - strand, within Pf6N2E2_2577at 3037.437 kb on + strand, within Pf6N2E2_2577at 3037.446 kb on + strand, within Pf6N2E2_2577at 3037.446 kb on + strand, within Pf6N2E2_2577at 3037.446 kb on + strand, within Pf6N2E2_2577at 3037.446 kb on + strand, within Pf6N2E2_2577at 3037.447 kb on - strand, within Pf6N2E2_2577at 3037.530 kb on + strand, within Pf6N2E2_2577at 3037.614 kb on - strand, within Pf6N2E2_2577at 3037.614 kb on - strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.885 kb on + strandat 3037.886 kb on - strandat 3038.077 kb on + strand, within Pf6N2E2_2578at 3038.286 kb on + strand, within Pf6N2E2_2578at 3038.504 kb on + strand, within Pf6N2E2_2578at 3038.607 kb on + strandat 3038.669 kb on - strandat 3038.669 kb on - strandat 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.709 kb on - strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.862 kb on - strand, within Pf6N2E2_2579at 3038.903 kb on + strandat 3038.903 kb on + strandat 3038.904 kb on - strandat 3038.917 kb on + strandat 3038.942 kb on + strandat 3038.943 kb on - strandat 3039.207 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,036,884 + Pf6N2E2_2577 0.14 -0.6
3,036,888 - Pf6N2E2_2577 0.15 -1.1
3,037,019 + Pf6N2E2_2577 0.26 -2.5
3,037,020 - Pf6N2E2_2577 0.26 -2.6
3,037,020 - Pf6N2E2_2577 0.26 -2.8
3,037,037 + Pf6N2E2_2577 0.28 -1.5
3,037,175 - Pf6N2E2_2577 0.40 -2.8
3,037,214 + Pf6N2E2_2577 0.43 -4.5
3,037,215 - Pf6N2E2_2577 0.43 -2.4
3,037,215 - Pf6N2E2_2577 0.43 -3.6
3,037,300 - Pf6N2E2_2577 0.51 -1.6
3,037,437 + Pf6N2E2_2577 0.63 -1.6
3,037,446 + Pf6N2E2_2577 0.64 -2.2
3,037,446 + Pf6N2E2_2577 0.64 -2.4
3,037,446 + Pf6N2E2_2577 0.64 -1.3
3,037,446 + Pf6N2E2_2577 0.64 +0.2
3,037,447 - Pf6N2E2_2577 0.64 -1.9
3,037,530 + Pf6N2E2_2577 0.71 -1.6
3,037,614 - Pf6N2E2_2577 0.79 -2.6
3,037,614 - Pf6N2E2_2577 0.79 -3.3
3,037,709 + Pf6N2E2_2577 0.87 -0.7
3,037,709 + Pf6N2E2_2577 0.87 -3.9
3,037,709 + Pf6N2E2_2577 0.87 -1.7
3,037,709 + Pf6N2E2_2577 0.87 -2.8
3,037,709 + Pf6N2E2_2577 0.87 -2.1
3,037,709 + Pf6N2E2_2577 0.87 +0.1
3,037,709 + Pf6N2E2_2577 0.87 -3.8
3,037,710 - Pf6N2E2_2577 0.87 -3.6
3,037,710 - Pf6N2E2_2577 0.87 -1.4
3,037,710 - Pf6N2E2_2577 0.87 -3.6
3,037,885 + -3.7
3,037,886 - -2.2
3,038,077 + Pf6N2E2_2578 0.28 -2.1
3,038,286 + Pf6N2E2_2578 0.54 -4.1
3,038,504 + Pf6N2E2_2578 0.82 -3.0
3,038,607 + -2.5
3,038,669 - -3.1
3,038,669 - -3.3
3,038,677 - Pf6N2E2_2579 0.11 -2.1
3,038,677 - Pf6N2E2_2579 0.11 -1.2
3,038,691 + Pf6N2E2_2579 0.16 +0.2
3,038,691 + Pf6N2E2_2579 0.16 -1.6
3,038,691 + Pf6N2E2_2579 0.16 -1.7
3,038,691 + Pf6N2E2_2579 0.16 -2.4
3,038,692 - Pf6N2E2_2579 0.16 -2.1
3,038,692 - Pf6N2E2_2579 0.16 -2.9
3,038,692 - Pf6N2E2_2579 0.16 -2.9
3,038,692 - Pf6N2E2_2579 0.16 -3.8
3,038,709 - Pf6N2E2_2579 0.23 -3.0
3,038,861 + Pf6N2E2_2579 0.79 -2.8
3,038,861 + Pf6N2E2_2579 0.79 -0.7
3,038,862 - Pf6N2E2_2579 0.79 -2.7
3,038,903 + -0.7
3,038,903 + -2.5
3,038,904 - -1.2
3,038,917 + -2.0
3,038,942 + -0.8
3,038,943 - +1.3
3,039,207 + Pf6N2E2_2580 0.35 -2.8
3,039,335 + Pf6N2E2_2580 0.53 -1.9
3,039,335 + Pf6N2E2_2580 0.53 -2.0
3,039,336 - Pf6N2E2_2580 0.53 -1.2
3,039,336 - Pf6N2E2_2580 0.53 -1.5
3,039,569 + Pf6N2E2_2580 0.84 -2.8
3,039,569 + Pf6N2E2_2580 0.84 -2.0

Or see this region's nucleotide sequence