Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2577 and Pf6N2E2_2578 are separated by 3 nucleotides Pf6N2E2_2578 and Pf6N2E2_2579 are separated by 4 nucleotides Pf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotides
Pf6N2E2_2577 - Flagellar biosynthesis protein FlhB, at 3,036,721 to 3,037,857
_2577
Pf6N2E2_2578 - Flagellar biosynthesis protein FliR, at 3,037,861 to 3,038,643
_2578
Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917
_2579
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3036.884 kb on + strand, within Pf6N2E2_2577 at 3036.888 kb on - strand, within Pf6N2E2_2577 at 3037.019 kb on + strand, within Pf6N2E2_2577 at 3037.020 kb on - strand, within Pf6N2E2_2577 at 3037.020 kb on - strand, within Pf6N2E2_2577 at 3037.037 kb on + strand, within Pf6N2E2_2577 at 3037.175 kb on - strand, within Pf6N2E2_2577 at 3037.214 kb on + strand, within Pf6N2E2_2577 at 3037.215 kb on - strand, within Pf6N2E2_2577 at 3037.215 kb on - strand, within Pf6N2E2_2577 at 3037.300 kb on - strand, within Pf6N2E2_2577 at 3037.437 kb on + strand, within Pf6N2E2_2577 at 3037.446 kb on + strand, within Pf6N2E2_2577 at 3037.446 kb on + strand, within Pf6N2E2_2577 at 3037.446 kb on + strand, within Pf6N2E2_2577 at 3037.446 kb on + strand, within Pf6N2E2_2577 at 3037.447 kb on - strand, within Pf6N2E2_2577 at 3037.530 kb on + strand, within Pf6N2E2_2577 at 3037.614 kb on - strand, within Pf6N2E2_2577 at 3037.614 kb on - strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.709 kb on + strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.710 kb on - strand, within Pf6N2E2_2577 at 3037.885 kb on + strand at 3037.886 kb on - strand at 3038.077 kb on + strand, within Pf6N2E2_2578 at 3038.286 kb on + strand, within Pf6N2E2_2578 at 3038.504 kb on + strand, within Pf6N2E2_2578 at 3038.607 kb on + strand at 3038.669 kb on - strand at 3038.669 kb on - strand at 3038.677 kb on - strand, within Pf6N2E2_2579 at 3038.677 kb on - strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.691 kb on + strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.692 kb on - strand, within Pf6N2E2_2579 at 3038.709 kb on - strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.861 kb on + strand, within Pf6N2E2_2579 at 3038.862 kb on - strand, within Pf6N2E2_2579 at 3038.903 kb on + strand at 3038.903 kb on + strand at 3038.904 kb on - strand at 3038.917 kb on + strand at 3038.942 kb on + strand at 3038.943 kb on - strand at 3039.207 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,036,884 + Pf6N2E2_2577 0.14 -0.6 3,036,888 - Pf6N2E2_2577 0.15 -1.1 3,037,019 + Pf6N2E2_2577 0.26 -2.5 3,037,020 - Pf6N2E2_2577 0.26 -2.6 3,037,020 - Pf6N2E2_2577 0.26 -2.8 3,037,037 + Pf6N2E2_2577 0.28 -1.5 3,037,175 - Pf6N2E2_2577 0.40 -2.8 3,037,214 + Pf6N2E2_2577 0.43 -4.5 3,037,215 - Pf6N2E2_2577 0.43 -2.4 3,037,215 - Pf6N2E2_2577 0.43 -3.6 3,037,300 - Pf6N2E2_2577 0.51 -1.6 3,037,437 + Pf6N2E2_2577 0.63 -1.6 3,037,446 + Pf6N2E2_2577 0.64 -2.2 3,037,446 + Pf6N2E2_2577 0.64 -2.4 3,037,446 + Pf6N2E2_2577 0.64 -1.3 3,037,446 + Pf6N2E2_2577 0.64 +0.2 3,037,447 - Pf6N2E2_2577 0.64 -1.9 3,037,530 + Pf6N2E2_2577 0.71 -1.6 3,037,614 - Pf6N2E2_2577 0.79 -2.6 3,037,614 - Pf6N2E2_2577 0.79 -3.3 3,037,709 + Pf6N2E2_2577 0.87 -0.7 3,037,709 + Pf6N2E2_2577 0.87 -3.9 3,037,709 + Pf6N2E2_2577 0.87 -1.7 3,037,709 + Pf6N2E2_2577 0.87 -2.8 3,037,709 + Pf6N2E2_2577 0.87 -2.1 3,037,709 + Pf6N2E2_2577 0.87 +0.1 3,037,709 + Pf6N2E2_2577 0.87 -3.8 3,037,710 - Pf6N2E2_2577 0.87 -3.6 3,037,710 - Pf6N2E2_2577 0.87 -1.4 3,037,710 - Pf6N2E2_2577 0.87 -3.6 3,037,885 + -3.7 3,037,886 - -2.2 3,038,077 + Pf6N2E2_2578 0.28 -2.1 3,038,286 + Pf6N2E2_2578 0.54 -4.1 3,038,504 + Pf6N2E2_2578 0.82 -3.0 3,038,607 + -2.5 3,038,669 - -3.1 3,038,669 - -3.3 3,038,677 - Pf6N2E2_2579 0.11 -2.1 3,038,677 - Pf6N2E2_2579 0.11 -1.2 3,038,691 + Pf6N2E2_2579 0.16 +0.2 3,038,691 + Pf6N2E2_2579 0.16 -1.6 3,038,691 + Pf6N2E2_2579 0.16 -1.7 3,038,691 + Pf6N2E2_2579 0.16 -2.4 3,038,692 - Pf6N2E2_2579 0.16 -2.1 3,038,692 - Pf6N2E2_2579 0.16 -2.9 3,038,692 - Pf6N2E2_2579 0.16 -2.9 3,038,692 - Pf6N2E2_2579 0.16 -3.8 3,038,709 - Pf6N2E2_2579 0.23 -3.0 3,038,861 + Pf6N2E2_2579 0.79 -2.8 3,038,861 + Pf6N2E2_2579 0.79 -0.7 3,038,862 - Pf6N2E2_2579 0.79 -2.7 3,038,903 + -0.7 3,038,903 + -2.5 3,038,904 - -1.2 3,038,917 + -2.0 3,038,942 + -0.8 3,038,943 - +1.3 3,039,207 + Pf6N2E2_2580 0.35 -2.8 3,039,335 + Pf6N2E2_2580 0.53 -1.9 3,039,335 + Pf6N2E2_2580 0.53 -2.0 3,039,336 - Pf6N2E2_2580 0.53 -1.2 3,039,336 - Pf6N2E2_2580 0.53 -1.5 3,039,569 + Pf6N2E2_2580 0.84 -2.8 3,039,569 + Pf6N2E2_2580 0.84 -2.0
Or see this region's nucleotide sequence