Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2575 and Pf6N2E2_2576 are separated by 11 nucleotides Pf6N2E2_2576 and Pf6N2E2_2577 are separated by 337 nucleotides
Pf6N2E2_2575 - Flagellar biosynthesis protein FlhF, at 3,032,890 to 3,034,239
_2575
Pf6N2E2_2576 - Flagellar biosynthesis protein FlhA, at 3,034,251 to 3,036,383
_2576
Pf6N2E2_2577 - Flagellar biosynthesis protein FlhB, at 3,036,721 to 3,037,857
_2577
Position (kb)
3034
3035
3036
3037 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3033.308 kb on - strand, within Pf6N2E2_2575 at 3033.308 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3033.479 kb on - strand, within Pf6N2E2_2575 at 3034.102 kb on - strand, within Pf6N2E2_2575 at 3034.102 kb on - strand, within Pf6N2E2_2575 at 3034.104 kb on - strand, within Pf6N2E2_2575 at 3034.240 kb on - strand at 3034.248 kb on - strand at 3034.313 kb on - strand at 3034.518 kb on - strand, within Pf6N2E2_2576 at 3034.528 kb on - strand, within Pf6N2E2_2576 at 3034.581 kb on + strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.582 kb on - strand, within Pf6N2E2_2576 at 3034.644 kb on - strand, within Pf6N2E2_2576 at 3034.686 kb on - strand, within Pf6N2E2_2576 at 3034.686 kb on - strand, within Pf6N2E2_2576 at 3034.686 kb on - strand, within Pf6N2E2_2576 at 3034.686 kb on - strand, within Pf6N2E2_2576 at 3034.692 kb on + strand, within Pf6N2E2_2576 at 3034.692 kb on + strand, within Pf6N2E2_2576 at 3034.692 kb on + strand, within Pf6N2E2_2576 at 3034.692 kb on + strand, within Pf6N2E2_2576 at 3034.692 kb on + strand, within Pf6N2E2_2576 at 3034.693 kb on - strand, within Pf6N2E2_2576 at 3034.693 kb on - strand, within Pf6N2E2_2576 at 3034.693 kb on - strand, within Pf6N2E2_2576 at 3034.693 kb on - strand, within Pf6N2E2_2576 at 3034.693 kb on - strand, within Pf6N2E2_2576 at 3034.810 kb on - strand, within Pf6N2E2_2576 at 3034.864 kb on + strand, within Pf6N2E2_2576 at 3034.864 kb on + strand, within Pf6N2E2_2576 at 3034.864 kb on + strand, within Pf6N2E2_2576 at 3034.864 kb on + strand, within Pf6N2E2_2576 at 3034.898 kb on + strand, within Pf6N2E2_2576 at 3034.917 kb on - strand, within Pf6N2E2_2576 at 3034.919 kb on - strand, within Pf6N2E2_2576 at 3035.017 kb on + strand, within Pf6N2E2_2576 at 3035.017 kb on + strand, within Pf6N2E2_2576 at 3035.056 kb on + strand, within Pf6N2E2_2576 at 3035.057 kb on - strand, within Pf6N2E2_2576 at 3035.407 kb on + strand, within Pf6N2E2_2576 at 3035.408 kb on - strand, within Pf6N2E2_2576 at 3035.408 kb on - strand, within Pf6N2E2_2576 at 3035.408 kb on - strand, within Pf6N2E2_2576 at 3035.408 kb on - strand, within Pf6N2E2_2576 at 3035.656 kb on + strand, within Pf6N2E2_2576 at 3035.656 kb on + strand, within Pf6N2E2_2576 at 3035.738 kb on + strand, within Pf6N2E2_2576 at 3035.738 kb on + strand, within Pf6N2E2_2576 at 3035.739 kb on - strand, within Pf6N2E2_2576 at 3035.797 kb on + strand, within Pf6N2E2_2576 at 3035.797 kb on + strand, within Pf6N2E2_2576 at 3035.916 kb on - strand, within Pf6N2E2_2576 at 3035.916 kb on - strand, within Pf6N2E2_2576 at 3036.013 kb on + strand, within Pf6N2E2_2576 at 3036.013 kb on + strand, within Pf6N2E2_2576 at 3036.013 kb on + strand, within Pf6N2E2_2576 at 3036.014 kb on - strand, within Pf6N2E2_2576 at 3036.014 kb on - strand, within Pf6N2E2_2576 at 3036.014 kb on - strand, within Pf6N2E2_2576 at 3036.014 kb on - strand, within Pf6N2E2_2576 at 3036.014 kb on - strand, within Pf6N2E2_2576 at 3036.190 kb on + strand at 3036.190 kb on + strand at 3036.191 kb on - strand at 3036.191 kb on - strand at 3036.191 kb on - strand at 3036.191 kb on - strand at 3036.192 kb on + strand at 3036.192 kb on + strand at 3036.192 kb on + strand at 3036.192 kb on + strand at 3036.192 kb on + strand at 3036.192 kb on + strand at 3036.193 kb on - strand at 3036.193 kb on - strand at 3036.193 kb on - strand at 3036.193 kb on - strand at 3036.336 kb on + strand at 3036.384 kb on + strand at 3036.384 kb on + strand at 3036.384 kb on + strand at 3036.385 kb on - strand at 3036.439 kb on + strand at 3036.473 kb on - strand at 3036.488 kb on + strand at 3036.539 kb on - strand at 3036.539 kb on - strand at 3036.539 kb on - strand at 3036.586 kb on - strand at 3036.642 kb on - strand at 3036.642 kb on - strand at 3036.713 kb on - strand at 3036.813 kb on - strand at 3036.818 kb on + strand at 3036.818 kb on + strand at 3036.845 kb on + strand, within Pf6N2E2_2577 at 3036.846 kb on - strand, within Pf6N2E2_2577 at 3036.884 kb on + strand, within Pf6N2E2_2577 at 3036.888 kb on - strand, within Pf6N2E2_2577 at 3037.019 kb on + strand, within Pf6N2E2_2577 at 3037.020 kb on - strand, within Pf6N2E2_2577 at 3037.020 kb on - strand, within Pf6N2E2_2577 at 3037.037 kb on + strand, within Pf6N2E2_2577 at 3037.175 kb on - strand, within Pf6N2E2_2577 at 3037.214 kb on + strand, within Pf6N2E2_2577 at 3037.215 kb on - strand, within Pf6N2E2_2577 at 3037.215 kb on - strand, within Pf6N2E2_2577 at 3037.300 kb on - strand, within Pf6N2E2_2577
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,033,308 - Pf6N2E2_2575 0.31 -3.2 3,033,308 - Pf6N2E2_2575 0.31 -2.4 3,033,479 - Pf6N2E2_2575 0.44 -4.4 3,033,479 - Pf6N2E2_2575 0.44 -3.0 3,033,479 - Pf6N2E2_2575 0.44 -2.8 3,033,479 - Pf6N2E2_2575 0.44 -2.6 3,033,479 - Pf6N2E2_2575 0.44 -2.7 3,033,479 - Pf6N2E2_2575 0.44 -3.2 3,033,479 - Pf6N2E2_2575 0.44 -1.2 3,033,479 - Pf6N2E2_2575 0.44 -3.7 3,034,102 - Pf6N2E2_2575 0.90 +0.1 3,034,102 - Pf6N2E2_2575 0.90 -2.8 3,034,104 - Pf6N2E2_2575 0.90 -2.5 3,034,240 - -1.0 3,034,248 - -2.8 3,034,313 - -2.3 3,034,518 - Pf6N2E2_2576 0.13 -2.5 3,034,528 - Pf6N2E2_2576 0.13 -1.7 3,034,581 + Pf6N2E2_2576 0.15 +2.7 3,034,582 - Pf6N2E2_2576 0.16 -3.3 3,034,582 - Pf6N2E2_2576 0.16 -3.4 3,034,582 - Pf6N2E2_2576 0.16 -3.2 3,034,582 - Pf6N2E2_2576 0.16 -0.4 3,034,582 - Pf6N2E2_2576 0.16 -4.0 3,034,582 - Pf6N2E2_2576 0.16 -3.3 3,034,582 - Pf6N2E2_2576 0.16 -1.8 3,034,644 - Pf6N2E2_2576 0.18 -2.2 3,034,686 - Pf6N2E2_2576 0.20 -2.1 3,034,686 - Pf6N2E2_2576 0.20 -3.2 3,034,686 - Pf6N2E2_2576 0.20 -3.1 3,034,686 - Pf6N2E2_2576 0.20 -3.0 3,034,692 + Pf6N2E2_2576 0.21 -2.9 3,034,692 + Pf6N2E2_2576 0.21 -3.0 3,034,692 + Pf6N2E2_2576 0.21 -2.7 3,034,692 + Pf6N2E2_2576 0.21 -2.9 3,034,692 + Pf6N2E2_2576 0.21 -1.8 3,034,693 - Pf6N2E2_2576 0.21 -0.7 3,034,693 - Pf6N2E2_2576 0.21 -2.3 3,034,693 - Pf6N2E2_2576 0.21 -1.3 3,034,693 - Pf6N2E2_2576 0.21 -2.3 3,034,693 - Pf6N2E2_2576 0.21 -3.1 3,034,810 - Pf6N2E2_2576 0.26 -2.7 3,034,864 + Pf6N2E2_2576 0.29 -3.6 3,034,864 + Pf6N2E2_2576 0.29 -2.2 3,034,864 + Pf6N2E2_2576 0.29 -3.0 3,034,864 + Pf6N2E2_2576 0.29 -1.8 3,034,898 + Pf6N2E2_2576 0.30 -0.4 3,034,917 - Pf6N2E2_2576 0.31 -1.9 3,034,919 - Pf6N2E2_2576 0.31 +0.1 3,035,017 + Pf6N2E2_2576 0.36 -3.1 3,035,017 + Pf6N2E2_2576 0.36 -3.2 3,035,056 + Pf6N2E2_2576 0.38 -2.0 3,035,057 - Pf6N2E2_2576 0.38 +1.1 3,035,407 + Pf6N2E2_2576 0.54 -2.8 3,035,408 - Pf6N2E2_2576 0.54 -2.7 3,035,408 - Pf6N2E2_2576 0.54 -3.2 3,035,408 - Pf6N2E2_2576 0.54 -1.6 3,035,408 - Pf6N2E2_2576 0.54 -4.6 3,035,656 + Pf6N2E2_2576 0.66 -4.0 3,035,656 + Pf6N2E2_2576 0.66 -2.9 3,035,738 + Pf6N2E2_2576 0.70 -0.9 3,035,738 + Pf6N2E2_2576 0.70 -3.2 3,035,739 - Pf6N2E2_2576 0.70 -2.7 3,035,797 + Pf6N2E2_2576 0.72 -1.8 3,035,797 + Pf6N2E2_2576 0.72 -2.0 3,035,916 - Pf6N2E2_2576 0.78 -1.9 3,035,916 - Pf6N2E2_2576 0.78 -4.4 3,036,013 + Pf6N2E2_2576 0.83 -1.9 3,036,013 + Pf6N2E2_2576 0.83 -3.2 3,036,013 + Pf6N2E2_2576 0.83 -2.7 3,036,014 - Pf6N2E2_2576 0.83 -3.0 3,036,014 - Pf6N2E2_2576 0.83 -4.3 3,036,014 - Pf6N2E2_2576 0.83 -2.7 3,036,014 - Pf6N2E2_2576 0.83 -2.2 3,036,014 - Pf6N2E2_2576 0.83 -3.1 3,036,190 + -2.4 3,036,190 + -1.9 3,036,191 - -2.4 3,036,191 - -2.0 3,036,191 - -2.5 3,036,191 - -1.8 3,036,192 + -1.5 3,036,192 + -3.1 3,036,192 + -0.6 3,036,192 + -3.9 3,036,192 + -0.4 3,036,192 + -1.5 3,036,193 - -1.0 3,036,193 - -3.1 3,036,193 - -1.5 3,036,193 - -2.5 3,036,336 + -2.9 3,036,384 + -3.3 3,036,384 + -1.3 3,036,384 + -3.3 3,036,385 - -2.6 3,036,439 + -1.1 3,036,473 - -1.3 3,036,488 + +0.6 3,036,539 - -0.5 3,036,539 - +1.1 3,036,539 - -2.3 3,036,586 - +0.4 3,036,642 - +1.6 3,036,642 - -1.5 3,036,713 - +0.2 3,036,813 - -0.9 3,036,818 + -1.5 3,036,818 + -2.8 3,036,845 + Pf6N2E2_2577 0.11 -1.6 3,036,846 - Pf6N2E2_2577 0.11 -1.6 3,036,884 + Pf6N2E2_2577 0.14 -0.6 3,036,888 - Pf6N2E2_2577 0.15 -1.1 3,037,019 + Pf6N2E2_2577 0.26 -2.5 3,037,020 - Pf6N2E2_2577 0.26 -2.6 3,037,020 - Pf6N2E2_2577 0.26 -2.8 3,037,037 + Pf6N2E2_2577 0.28 -1.5 3,037,175 - Pf6N2E2_2577 0.40 -2.8 3,037,214 + Pf6N2E2_2577 0.43 -4.5 3,037,215 - Pf6N2E2_2577 0.43 -2.4 3,037,215 - Pf6N2E2_2577 0.43 -3.6 3,037,300 - Pf6N2E2_2577 0.51 -1.6
Or see this region's nucleotide sequence