Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2561 and Pf6N2E2_2562 overlap by 4 nucleotides Pf6N2E2_2562 and Pf6N2E2_2563 are separated by 252 nucleotides Pf6N2E2_2563 and Pf6N2E2_2564 are separated by 1 nucleotides Pf6N2E2_2564 and Pf6N2E2_2565 are separated by 72 nucleotides
Pf6N2E2_2561 - FIG00955434: hypothetical protein, at 3,019,831 to 3,021,402
_2561
Pf6N2E2_2562 - FlhB domain protein, at 3,021,399 to 3,021,728
_2562
Pf6N2E2_2563 - FIG00953562: hypothetical protein, at 3,021,981 to 3,022,376
_2563
Pf6N2E2_2564 - Positive regulator of CheA protein activity (CheW), at 3,022,378 to 3,022,863
_2564
Pf6N2E2_2565 - CheW domain protein, at 3,022,936 to 3,023,874
_2565
Position (kb)
3021
3022
3023 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3021.223 kb on - strand, within Pf6N2E2_2561 at 3021.723 kb on + strand at 3021.724 kb on - strand at 3021.928 kb on + strand at 3021.928 kb on + strand at 3021.928 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.001 kb on + strand at 3022.002 kb on - strand at 3022.002 kb on - strand at 3022.002 kb on - strand at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.302 kb on + strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.303 kb on - strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.304 kb on + strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.305 kb on - strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.306 kb on + strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.307 kb on - strand, within Pf6N2E2_2563 at 3022.363 kb on - strand at 3022.363 kb on - strand at 3022.363 kb on - strand at 3022.384 kb on - strand at 3022.384 kb on - strand at 3022.384 kb on - strand at 3022.539 kb on - strand, within Pf6N2E2_2564 at 3022.706 kb on + strand, within Pf6N2E2_2564 at 3022.707 kb on - strand, within Pf6N2E2_2564 at 3022.707 kb on - strand, within Pf6N2E2_2564 at 3022.741 kb on - strand, within Pf6N2E2_2564 at 3022.742 kb on + strand, within Pf6N2E2_2564 at 3022.742 kb on + strand, within Pf6N2E2_2564 at 3022.781 kb on + strand, within Pf6N2E2_2564 at 3022.781 kb on + strand, within Pf6N2E2_2564 at 3022.782 kb on - strand, within Pf6N2E2_2564 at 3022.782 kb on - strand, within Pf6N2E2_2564 at 3022.854 kb on + strand at 3022.854 kb on + strand at 3022.863 kb on + strand at 3022.864 kb on - strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.900 kb on + strand at 3022.901 kb on - strand at 3022.901 kb on - strand at 3022.901 kb on - strand at 3022.937 kb on + strand at 3022.937 kb on + strand at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.037 kb on + strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.038 kb on - strand, within Pf6N2E2_2565 at 3023.052 kb on + strand, within Pf6N2E2_2565 at 3023.157 kb on - strand, within Pf6N2E2_2565 at 3023.177 kb on + strand, within Pf6N2E2_2565 at 3023.177 kb on + strand, within Pf6N2E2_2565 at 3023.178 kb on - strand, within Pf6N2E2_2565 at 3023.178 kb on - strand, within Pf6N2E2_2565
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,021,223 - Pf6N2E2_2561 0.89 -1.5 3,021,723 + -0.9 3,021,724 - -1.7 3,021,928 + -0.1 3,021,928 + -1.3 3,021,928 + -1.5 3,022,001 + -3.3 3,022,001 + -3.6 3,022,001 + +0.5 3,022,001 + -3.0 3,022,001 + -3.0 3,022,002 - -3.4 3,022,002 - -2.5 3,022,002 - -4.0 3,022,302 + Pf6N2E2_2563 0.81 -2.6 3,022,302 + Pf6N2E2_2563 0.81 -2.0 3,022,302 + Pf6N2E2_2563 0.81 -1.3 3,022,302 + Pf6N2E2_2563 0.81 -3.0 3,022,302 + Pf6N2E2_2563 0.81 -2.6 3,022,302 + Pf6N2E2_2563 0.81 -1.6 3,022,302 + Pf6N2E2_2563 0.81 -2.3 3,022,302 + Pf6N2E2_2563 0.81 -1.1 3,022,303 - Pf6N2E2_2563 0.81 -3.1 3,022,303 - Pf6N2E2_2563 0.81 -3.4 3,022,303 - Pf6N2E2_2563 0.81 -3.1 3,022,303 - Pf6N2E2_2563 0.81 -3.4 3,022,303 - Pf6N2E2_2563 0.81 -1.1 3,022,303 - Pf6N2E2_2563 0.81 -3.6 3,022,303 - Pf6N2E2_2563 0.81 -2.8 3,022,303 - Pf6N2E2_2563 0.81 -3.0 3,022,303 - Pf6N2E2_2563 0.81 -3.3 3,022,303 - Pf6N2E2_2563 0.81 -2.3 3,022,303 - Pf6N2E2_2563 0.81 -3.3 3,022,303 - Pf6N2E2_2563 0.81 -2.4 3,022,303 - Pf6N2E2_2563 0.81 -3.3 3,022,303 - Pf6N2E2_2563 0.81 -3.9 3,022,303 - Pf6N2E2_2563 0.81 -1.9 3,022,303 - Pf6N2E2_2563 0.81 -2.8 3,022,303 - Pf6N2E2_2563 0.81 -0.3 3,022,303 - Pf6N2E2_2563 0.81 -3.6 3,022,303 - Pf6N2E2_2563 0.81 -3.8 3,022,303 - Pf6N2E2_2563 0.81 -2.7 3,022,303 - Pf6N2E2_2563 0.81 -2.8 3,022,303 - Pf6N2E2_2563 0.81 +0.8 3,022,303 - Pf6N2E2_2563 0.81 -3.3 3,022,303 - Pf6N2E2_2563 0.81 -3.7 3,022,303 - Pf6N2E2_2563 0.81 -1.8 3,022,303 - Pf6N2E2_2563 0.81 -2.4 3,022,303 - Pf6N2E2_2563 0.81 -2.8 3,022,303 - Pf6N2E2_2563 0.81 -2.5 3,022,303 - Pf6N2E2_2563 0.81 -2.8 3,022,304 + Pf6N2E2_2563 0.82 -1.7 3,022,304 + Pf6N2E2_2563 0.82 -3.7 3,022,304 + Pf6N2E2_2563 0.82 -3.0 3,022,304 + Pf6N2E2_2563 0.82 -1.8 3,022,305 - Pf6N2E2_2563 0.82 -3.7 3,022,305 - Pf6N2E2_2563 0.82 -3.5 3,022,305 - Pf6N2E2_2563 0.82 -3.4 3,022,305 - Pf6N2E2_2563 0.82 -3.5 3,022,305 - Pf6N2E2_2563 0.82 -2.1 3,022,306 + Pf6N2E2_2563 0.82 -1.4 3,022,306 + Pf6N2E2_2563 0.82 -0.8 3,022,306 + Pf6N2E2_2563 0.82 +0.8 3,022,306 + Pf6N2E2_2563 0.82 -2.4 3,022,307 - Pf6N2E2_2563 0.82 -3.2 3,022,307 - Pf6N2E2_2563 0.82 -3.3 3,022,307 - Pf6N2E2_2563 0.82 -3.5 3,022,307 - Pf6N2E2_2563 0.82 -1.6 3,022,363 - -2.2 3,022,363 - -2.7 3,022,363 - +1.3 3,022,384 - -2.7 3,022,384 - -3.0 3,022,384 - -1.6 3,022,539 - Pf6N2E2_2564 0.33 -1.3 3,022,706 + Pf6N2E2_2564 0.67 -1.6 3,022,707 - Pf6N2E2_2564 0.68 -3.6 3,022,707 - Pf6N2E2_2564 0.68 -2.2 3,022,741 - Pf6N2E2_2564 0.75 -2.0 3,022,742 + Pf6N2E2_2564 0.75 -1.7 3,022,742 + Pf6N2E2_2564 0.75 -1.3 3,022,781 + Pf6N2E2_2564 0.83 -3.0 3,022,781 + Pf6N2E2_2564 0.83 -2.3 3,022,782 - Pf6N2E2_2564 0.83 -3.0 3,022,782 - Pf6N2E2_2564 0.83 -3.4 3,022,854 + -3.9 3,022,854 + -2.2 3,022,863 + -2.3 3,022,864 - -1.5 3,022,900 + -2.2 3,022,900 + -3.4 3,022,900 + -1.2 3,022,900 + -4.3 3,022,900 + -2.5 3,022,900 + -3.0 3,022,901 - +1.0 3,022,901 - -0.6 3,022,901 - -1.5 3,022,937 + -3.0 3,022,937 + -2.1 3,023,037 + Pf6N2E2_2565 0.11 -1.5 3,023,037 + Pf6N2E2_2565 0.11 -1.0 3,023,037 + Pf6N2E2_2565 0.11 -3.2 3,023,037 + Pf6N2E2_2565 0.11 -2.0 3,023,037 + Pf6N2E2_2565 0.11 -3.1 3,023,037 + Pf6N2E2_2565 0.11 -2.0 3,023,037 + Pf6N2E2_2565 0.11 -3.8 3,023,037 + Pf6N2E2_2565 0.11 -1.6 3,023,037 + Pf6N2E2_2565 0.11 -0.5 3,023,037 + Pf6N2E2_2565 0.11 -2.2 3,023,037 + Pf6N2E2_2565 0.11 -1.6 3,023,037 + Pf6N2E2_2565 0.11 -3.6 3,023,038 - Pf6N2E2_2565 0.11 -3.6 3,023,038 - Pf6N2E2_2565 0.11 -3.0 3,023,038 - Pf6N2E2_2565 0.11 -1.0 3,023,038 - Pf6N2E2_2565 0.11 -1.9 3,023,038 - Pf6N2E2_2565 0.11 -2.6 3,023,038 - Pf6N2E2_2565 0.11 -1.8 3,023,038 - Pf6N2E2_2565 0.11 -2.0 3,023,038 - Pf6N2E2_2565 0.11 -2.2 3,023,038 - Pf6N2E2_2565 0.11 -2.4 3,023,052 + Pf6N2E2_2565 0.12 -3.1 3,023,157 - Pf6N2E2_2565 0.24 -2.4 3,023,177 + Pf6N2E2_2565 0.26 -2.8 3,023,177 + Pf6N2E2_2565 0.26 -3.2 3,023,178 - Pf6N2E2_2565 0.26 -3.5 3,023,178 - Pf6N2E2_2565 0.26 -3.0
Or see this region's nucleotide sequence