Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2526

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2524 and Pf6N2E2_2525 are separated by 61 nucleotidesPf6N2E2_2525 and Pf6N2E2_2526 are separated by 4 nucleotidesPf6N2E2_2526 and Pf6N2E2_2527 are separated by 167 nucleotides Pf6N2E2_2524 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 2,981,025 to 2,982,356 _2524 Pf6N2E2_2525 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 2,982,418 to 2,983,116 _2525 Pf6N2E2_2526 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 2,983,121 to 2,983,792 _2526 Pf6N2E2_2527 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,983,960 to 2,984,700 _2527 Position (kb) 2983 2984Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2982.186 kb on + strand, within Pf6N2E2_2524at 2982.186 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.210 kb on + strand, within Pf6N2E2_2524at 2982.232 kb on + strandat 2982.354 kb on + strandat 2983.114 kb on + strandat 2983.114 kb on + strandat 2983.115 kb on - strandat 2983.115 kb on - strandat 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.682 kb on + strand, within Pf6N2E2_2526at 2983.683 kb on - strand, within Pf6N2E2_2526at 2983.683 kb on - strand, within Pf6N2E2_2526at 2983.688 kb on + strand, within Pf6N2E2_2526at 2983.689 kb on - strand, within Pf6N2E2_2526at 2983.942 kb on + strandat 2983.942 kb on + strandat 2983.942 kb on + strandat 2983.959 kb on + strandat 2983.959 kb on + strandat 2983.960 kb on - strandat 2984.057 kb on - strand, within Pf6N2E2_2527at 2984.119 kb on + strand, within Pf6N2E2_2527at 2984.119 kb on + strand, within Pf6N2E2_2527at 2984.197 kb on + strand, within Pf6N2E2_2527at 2984.197 kb on + strand, within Pf6N2E2_2527at 2984.198 kb on - strand, within Pf6N2E2_2527at 2984.198 kb on - strand, within Pf6N2E2_2527at 2984.280 kb on + strand, within Pf6N2E2_2527at 2984.355 kb on + strand, within Pf6N2E2_2527at 2984.444 kb on - strand, within Pf6N2E2_2527at 2984.455 kb on + strand, within Pf6N2E2_2527at 2984.644 kb on + strandat 2984.727 kb on + strandat 2984.727 kb on + strandat 2984.728 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,982,186 + Pf6N2E2_2524 0.87 -2.7
2,982,186 + Pf6N2E2_2524 0.87 -2.0
2,982,210 + Pf6N2E2_2524 0.89 +0.2
2,982,210 + Pf6N2E2_2524 0.89 -2.0
2,982,210 + Pf6N2E2_2524 0.89 -2.1
2,982,210 + Pf6N2E2_2524 0.89 -0.6
2,982,210 + Pf6N2E2_2524 0.89 -1.6
2,982,210 + Pf6N2E2_2524 0.89 -1.7
2,982,210 + Pf6N2E2_2524 0.89 -1.4
2,982,210 + Pf6N2E2_2524 0.89 -0.1
2,982,210 + Pf6N2E2_2524 0.89 -1.3
2,982,210 + Pf6N2E2_2524 0.89 -2.6
2,982,232 + -0.7
2,982,354 + +4.6
2,983,114 + -1.6
2,983,114 + +0.7
2,983,115 - -0.6
2,983,115 - -2.5
2,983,682 + Pf6N2E2_2526 0.83 -1.3
2,983,682 + Pf6N2E2_2526 0.83 -1.4
2,983,682 + Pf6N2E2_2526 0.83 -0.3
2,983,682 + Pf6N2E2_2526 0.83 -2.6
2,983,682 + Pf6N2E2_2526 0.83 -0.8
2,983,682 + Pf6N2E2_2526 0.83 -1.5
2,983,683 - Pf6N2E2_2526 0.84 -2.5
2,983,683 - Pf6N2E2_2526 0.84 -1.8
2,983,688 + Pf6N2E2_2526 0.84 -0.4
2,983,689 - Pf6N2E2_2526 0.85 -3.1
2,983,942 + +0.7
2,983,942 + -1.2
2,983,942 + -0.6
2,983,959 + -0.8
2,983,959 + -0.2
2,983,960 - -1.8
2,984,057 - Pf6N2E2_2527 0.13 -1.0
2,984,119 + Pf6N2E2_2527 0.21 -1.4
2,984,119 + Pf6N2E2_2527 0.21 -1.7
2,984,197 + Pf6N2E2_2527 0.32 -1.0
2,984,197 + Pf6N2E2_2527 0.32 -2.5
2,984,198 - Pf6N2E2_2527 0.32 -1.0
2,984,198 - Pf6N2E2_2527 0.32 -1.4
2,984,280 + Pf6N2E2_2527 0.43 -2.6
2,984,355 + Pf6N2E2_2527 0.53 -1.4
2,984,444 - Pf6N2E2_2527 0.65 -2.1
2,984,455 + Pf6N2E2_2527 0.67 -0.5
2,984,644 + -0.4
2,984,727 + +1.4
2,984,727 + -0.6
2,984,728 - +1.5

Or see this region's nucleotide sequence