Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2519

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2518 and Pf6N2E2_2519 are separated by 28 nucleotidesPf6N2E2_2519 and Pf6N2E2_2520 are separated by 13 nucleotides Pf6N2E2_2518 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 2,970,891 to 2,973,098 _2518 Pf6N2E2_2519 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 2,973,127 to 2,974,239 _2519 Pf6N2E2_2520 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 2,974,253 to 2,975,347 _2520 Position (kb) 2973 2974 2975Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2972.225 kb on - strand, within Pf6N2E2_2518at 2972.225 kb on - strand, within Pf6N2E2_2518at 2972.355 kb on - strand, within Pf6N2E2_2518at 2972.355 kb on - strand, within Pf6N2E2_2518at 2973.099 kb on - strandat 2973.099 kb on - strandat 2973.191 kb on - strandat 2973.191 kb on - strandat 2973.222 kb on - strandat 2973.222 kb on - strandat 2973.222 kb on - strandat 2973.222 kb on - strandat 2973.222 kb on - strandat 2973.226 kb on - strandat 2973.226 kb on - strandat 2973.546 kb on - strand, within Pf6N2E2_2519

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,972,225 - Pf6N2E2_2518 0.60 +0.5
2,972,225 - Pf6N2E2_2518 0.60 +1.9
2,972,355 - Pf6N2E2_2518 0.66 -2.2
2,972,355 - Pf6N2E2_2518 0.66 -3.6
2,973,099 - -0.6
2,973,099 - -2.3
2,973,191 - +0.2
2,973,191 - +0.4
2,973,222 - -1.1
2,973,222 - +0.9
2,973,222 - -1.1
2,973,222 - -0.7
2,973,222 - -0.0
2,973,226 - -1.7
2,973,226 - -1.4
2,973,546 - Pf6N2E2_2519 0.38 -1.8

Or see this region's nucleotide sequence