Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2484 and Pf6N2E2_2485 are separated by 258 nucleotides Pf6N2E2_2485 and Pf6N2E2_2486 are separated by 27 nucleotides Pf6N2E2_2486 and Pf6N2E2_2487 are separated by 348 nucleotides
Pf6N2E2_2484 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,930,401 to 2,931,882
_2484
Pf6N2E2_2485 - Phage terminase, small subunit, at 2,932,141 to 2,932,515
_2485
Pf6N2E2_2486 - TesB-like acyl-CoA thioesterase 1, at 2,932,543 to 2,933,340
_2486
Pf6N2E2_2487 - FIG00956090: hypothetical protein, at 2,933,689 to 2,934,450
_2487
Position (kb)
2932
2933
2934 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2931.765 kb on + strand at 2931.765 kb on + strand at 2931.766 kb on - strand at 2931.880 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.016 kb on - strand at 2932.044 kb on + strand at 2932.045 kb on - strand at 2932.055 kb on - strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.187 kb on - strand, within Pf6N2E2_2485 at 2932.658 kb on + strand, within Pf6N2E2_2486 at 2932.658 kb on + strand, within Pf6N2E2_2486 at 2932.658 kb on + strand, within Pf6N2E2_2486 at 2932.932 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.946 kb on + strand, within Pf6N2E2_2486 at 2932.947 kb on - strand, within Pf6N2E2_2486 at 2932.947 kb on - strand, within Pf6N2E2_2486 at 2932.947 kb on - strand, within Pf6N2E2_2486 at 2933.057 kb on - strand, within Pf6N2E2_2486 at 2933.116 kb on + strand, within Pf6N2E2_2486 at 2933.116 kb on + strand, within Pf6N2E2_2486 at 2933.117 kb on - strand, within Pf6N2E2_2486 at 2933.225 kb on + strand, within Pf6N2E2_2486 at 2933.225 kb on + strand, within Pf6N2E2_2486 at 2933.225 kb on + strand, within Pf6N2E2_2486 at 2933.225 kb on + strand, within Pf6N2E2_2486 at 2933.226 kb on - strand at 2933.467 kb on + strand at 2933.467 kb on + strand at 2933.468 kb on - strand at 2933.468 kb on - strand at 2933.468 kb on - strand at 2933.471 kb on - strand at 2933.471 kb on - strand at 2933.471 kb on - strand at 2933.471 kb on - strand at 2933.471 kb on - strand at 2933.482 kb on - strand at 2933.482 kb on - strand at 2933.482 kb on - strand at 2933.647 kb on + strand at 2934.146 kb on + strand, within Pf6N2E2_2487
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,931,765 + -0.5 2,931,765 + -0.2 2,931,766 - -1.1 2,931,880 + +1.4 2,932,015 + -0.8 2,932,015 + +1.0 2,932,015 + -0.4 2,932,015 + +0.6 2,932,015 + +0.3 2,932,015 + +1.0 2,932,016 - -0.2 2,932,044 + +0.4 2,932,045 - +0.5 2,932,055 - -0.8 2,932,134 + +0.2 2,932,134 + -1.7 2,932,134 + -0.6 2,932,134 + +0.2 2,932,187 - Pf6N2E2_2485 0.12 +1.0 2,932,658 + Pf6N2E2_2486 0.14 +1.0 2,932,658 + Pf6N2E2_2486 0.14 -0.3 2,932,658 + Pf6N2E2_2486 0.14 +1.3 2,932,932 + Pf6N2E2_2486 0.49 +1.0 2,932,946 + Pf6N2E2_2486 0.51 -1.0 2,932,946 + Pf6N2E2_2486 0.51 +0.9 2,932,946 + Pf6N2E2_2486 0.51 +1.9 2,932,946 + Pf6N2E2_2486 0.51 -2.0 2,932,946 + Pf6N2E2_2486 0.51 +1.0 2,932,946 + Pf6N2E2_2486 0.51 -0.2 2,932,947 - Pf6N2E2_2486 0.51 +1.9 2,932,947 - Pf6N2E2_2486 0.51 -0.9 2,932,947 - Pf6N2E2_2486 0.51 -0.2 2,933,057 - Pf6N2E2_2486 0.64 +0.2 2,933,116 + Pf6N2E2_2486 0.72 -0.1 2,933,116 + Pf6N2E2_2486 0.72 +1.7 2,933,117 - Pf6N2E2_2486 0.72 -0.9 2,933,225 + Pf6N2E2_2486 0.85 +1.2 2,933,225 + Pf6N2E2_2486 0.85 -0.2 2,933,225 + Pf6N2E2_2486 0.85 +1.6 2,933,225 + Pf6N2E2_2486 0.85 +1.4 2,933,226 - +3.5 2,933,467 + -0.2 2,933,467 + -2.1 2,933,468 - -1.5 2,933,468 - -0.3 2,933,468 - +0.3 2,933,471 - -0.6 2,933,471 - -0.4 2,933,471 - +0.9 2,933,471 - -0.4 2,933,471 - +0.2 2,933,482 - +0.3 2,933,482 - -1.5 2,933,482 - -1.8 2,933,647 + -0.2 2,934,146 + Pf6N2E2_2487 0.60 +1.2
Or see this region's nucleotide sequence