Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_248

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_247 and Pf6N2E2_248 are separated by 117 nucleotidesPf6N2E2_248 and Pf6N2E2_249 are separated by 384 nucleotides Pf6N2E2_247 - Transcriptional regulator, AraC family, at 308,616 to 309,659 _247 Pf6N2E2_248 - hypothetical protein, at 309,777 to 310,190 _248 Pf6N2E2_249 - Pirin, at 310,575 to 311,297 _249 Position (kb) 309 310 311Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 308.802 kb on + strand, within Pf6N2E2_247at 308.802 kb on + strand, within Pf6N2E2_247at 308.803 kb on - strand, within Pf6N2E2_247at 309.135 kb on + strand, within Pf6N2E2_247at 309.135 kb on + strand, within Pf6N2E2_247at 309.136 kb on - strand, within Pf6N2E2_247at 309.136 kb on - strand, within Pf6N2E2_247at 309.367 kb on + strand, within Pf6N2E2_247at 309.367 kb on + strand, within Pf6N2E2_247at 309.471 kb on + strand, within Pf6N2E2_247at 309.474 kb on + strand, within Pf6N2E2_247at 309.618 kb on - strandat 309.622 kb on - strandat 309.726 kb on + strandat 309.726 kb on + strandat 309.772 kb on - strandat 309.772 kb on - strandat 309.772 kb on - strandat 309.772 kb on - strandat 309.772 kb on - strandat 309.785 kb on + strandat 309.958 kb on + strand, within Pf6N2E2_248at 309.958 kb on + strand, within Pf6N2E2_248at 309.958 kb on + strand, within Pf6N2E2_248at 309.958 kb on + strand, within Pf6N2E2_248at 309.958 kb on + strand, within Pf6N2E2_248at 309.958 kb on + strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 309.959 kb on - strand, within Pf6N2E2_248at 310.013 kb on - strand, within Pf6N2E2_248at 310.024 kb on + strand, within Pf6N2E2_248at 310.025 kb on - strand, within Pf6N2E2_248at 310.025 kb on - strand, within Pf6N2E2_248at 310.025 kb on - strand, within Pf6N2E2_248at 310.041 kb on + strand, within Pf6N2E2_248at 310.042 kb on - strand, within Pf6N2E2_248at 310.042 kb on - strand, within Pf6N2E2_248at 310.090 kb on + strand, within Pf6N2E2_248at 310.090 kb on + strand, within Pf6N2E2_248at 310.090 kb on + strand, within Pf6N2E2_248at 310.111 kb on + strand, within Pf6N2E2_248at 310.111 kb on + strand, within Pf6N2E2_248at 310.112 kb on - strand, within Pf6N2E2_248at 310.112 kb on - strand, within Pf6N2E2_248at 310.117 kb on + strand, within Pf6N2E2_248at 310.117 kb on + strand, within Pf6N2E2_248at 310.117 kb on + strand, within Pf6N2E2_248at 310.117 kb on + strand, within Pf6N2E2_248at 310.118 kb on - strand, within Pf6N2E2_248at 310.158 kb on - strandat 310.176 kb on + strandat 310.177 kb on - strandat 310.177 kb on - strandat 310.177 kb on - strandat 310.179 kb on - strandat 310.206 kb on + strandat 310.207 kb on - strandat 310.227 kb on + strandat 310.328 kb on + strandat 310.393 kb on - strandat 310.449 kb on + strandat 310.450 kb on - strandat 310.450 kb on - strandat 310.469 kb on + strandat 310.469 kb on + strandat 310.469 kb on + strandat 310.509 kb on + strandat 310.510 kb on - strandat 310.517 kb on + strandat 310.517 kb on + strandat 310.518 kb on - strandat 310.526 kb on + strandat 310.699 kb on - strand, within Pf6N2E2_249at 310.738 kb on + strand, within Pf6N2E2_249at 310.738 kb on + strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.739 kb on - strand, within Pf6N2E2_249at 310.744 kb on + strand, within Pf6N2E2_249at 310.745 kb on - strand, within Pf6N2E2_249at 310.745 kb on - strand, within Pf6N2E2_249at 310.802 kb on - strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.098 kb on + strand, within Pf6N2E2_249at 311.099 kb on - strand, within Pf6N2E2_249

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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308,802 + Pf6N2E2_247 0.18 +0.1
308,802 + Pf6N2E2_247 0.18 +0.9
308,803 - Pf6N2E2_247 0.18 -0.1
309,135 + Pf6N2E2_247 0.50 -0.5
309,135 + Pf6N2E2_247 0.50 +1.5
309,136 - Pf6N2E2_247 0.50 +0.6
309,136 - Pf6N2E2_247 0.50 -1.4
309,367 + Pf6N2E2_247 0.72 +0.5
309,367 + Pf6N2E2_247 0.72 -0.1
309,471 + Pf6N2E2_247 0.82 +1.4
309,474 + Pf6N2E2_247 0.82 -0.8
309,618 - -0.5
309,622 - +0.7
309,726 + -0.9
309,726 + -2.0
309,772 - -0.0
309,772 - -2.1
309,772 - -2.9
309,772 - -0.0
309,772 - -0.2
309,785 + -2.4
309,958 + Pf6N2E2_248 0.44 -1.5
309,958 + Pf6N2E2_248 0.44 -1.6
309,958 + Pf6N2E2_248 0.44 -1.1
309,958 + Pf6N2E2_248 0.44 -0.9
309,958 + Pf6N2E2_248 0.44 -0.9
309,958 + Pf6N2E2_248 0.44 -0.8
309,959 - Pf6N2E2_248 0.44 -1.8
309,959 - Pf6N2E2_248 0.44 -0.9
309,959 - Pf6N2E2_248 0.44 -1.6
309,959 - Pf6N2E2_248 0.44 -2.3
309,959 - Pf6N2E2_248 0.44 -1.9
309,959 - Pf6N2E2_248 0.44 -1.4
309,959 - Pf6N2E2_248 0.44 -1.6
310,013 - Pf6N2E2_248 0.57 -2.9
310,024 + Pf6N2E2_248 0.60 -1.4
310,025 - Pf6N2E2_248 0.60 -1.7
310,025 - Pf6N2E2_248 0.60 -1.7
310,025 - Pf6N2E2_248 0.60 -0.7
310,041 + Pf6N2E2_248 0.64 -1.7
310,042 - Pf6N2E2_248 0.64 -1.9
310,042 - Pf6N2E2_248 0.64 -0.0
310,090 + Pf6N2E2_248 0.76 -0.2
310,090 + Pf6N2E2_248 0.76 -0.9
310,090 + Pf6N2E2_248 0.76 -1.7
310,111 + Pf6N2E2_248 0.81 -2.1
310,111 + Pf6N2E2_248 0.81 -1.9
310,112 - Pf6N2E2_248 0.81 -2.9
310,112 - Pf6N2E2_248 0.81 -1.5
310,117 + Pf6N2E2_248 0.82 -1.4
310,117 + Pf6N2E2_248 0.82 -1.0
310,117 + Pf6N2E2_248 0.82 -2.2
310,117 + Pf6N2E2_248 0.82 +0.1
310,118 - Pf6N2E2_248 0.82 -1.6
310,158 - -0.2
310,176 + -0.8
310,177 - -2.9
310,177 - -1.4
310,177 - -1.7
310,179 - -1.2
310,206 + -2.5
310,207 - -0.3
310,227 + -0.2
310,328 + -0.3
310,393 - +0.3
310,449 + -0.2
310,450 - -1.0
310,450 - +2.3
310,469 + -0.1
310,469 + +1.0
310,469 + -1.6
310,509 + -0.7
310,510 - -0.3
310,517 + -0.6
310,517 + -0.0
310,518 - +1.0
310,526 + -1.8
310,699 - Pf6N2E2_249 0.17 -0.6
310,738 + Pf6N2E2_249 0.23 -1.3
310,738 + Pf6N2E2_249 0.23 -0.2
310,739 - Pf6N2E2_249 0.23 -1.9
310,739 - Pf6N2E2_249 0.23 -3.1
310,739 - Pf6N2E2_249 0.23 +0.1
310,739 - Pf6N2E2_249 0.23 +1.0
310,744 + Pf6N2E2_249 0.23 -2.3
310,745 - Pf6N2E2_249 0.24 +0.2
310,745 - Pf6N2E2_249 0.24 +0.5
310,802 - Pf6N2E2_249 0.31 -0.9
311,098 + Pf6N2E2_249 0.72 +0.3
311,098 + Pf6N2E2_249 0.72 +0.8
311,098 + Pf6N2E2_249 0.72 +0.2
311,099 - Pf6N2E2_249 0.72 -0.5

Or see this region's nucleotide sequence