Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2399

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2398 and Pf6N2E2_2399 overlap by 1 nucleotidesPf6N2E2_2399 and Pf6N2E2_2400 overlap by 25 nucleotidesPf6N2E2_2400 and Pf6N2E2_2401 are separated by 152 nucleotides Pf6N2E2_2398 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 2,837,019 to 2,838,746 _2398 Pf6N2E2_2399 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 2,838,746 to 2,839,615 _2399 Pf6N2E2_2400 - Thiol:disulfide interchange protein DsbG precursor, at 2,839,591 to 2,840,358 _2400 Pf6N2E2_2401 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 2,840,511 to 2,841,323 _2401 Position (kb) 2838 2839 2840Strain fitness (log2 ratio) -3 -2 -1 0 1at 2838.874 kb on - strand, within Pf6N2E2_2399at 2838.874 kb on - strand, within Pf6N2E2_2399at 2839.133 kb on + strand, within Pf6N2E2_2399at 2839.133 kb on + strand, within Pf6N2E2_2399at 2839.233 kb on + strand, within Pf6N2E2_2399at 2839.234 kb on - strand, within Pf6N2E2_2399at 2839.234 kb on - strand, within Pf6N2E2_2399at 2839.234 kb on - strand, within Pf6N2E2_2399at 2839.234 kb on - strand, within Pf6N2E2_2399at 2839.234 kb on - strand, within Pf6N2E2_2399at 2839.358 kb on + strand, within Pf6N2E2_2399at 2839.358 kb on + strand, within Pf6N2E2_2399at 2839.359 kb on - strand, within Pf6N2E2_2399at 2839.359 kb on - strand, within Pf6N2E2_2399at 2839.359 kb on - strand, within Pf6N2E2_2399at 2839.359 kb on - strand, within Pf6N2E2_2399at 2839.460 kb on + strand, within Pf6N2E2_2399at 2839.460 kb on + strand, within Pf6N2E2_2399at 2839.461 kb on - strand, within Pf6N2E2_2399at 2839.461 kb on - strand, within Pf6N2E2_2399at 2839.762 kb on + strand, within Pf6N2E2_2400at 2839.763 kb on - strand, within Pf6N2E2_2400at 2839.786 kb on + strand, within Pf6N2E2_2400at 2839.787 kb on - strand, within Pf6N2E2_2400at 2839.829 kb on - strand, within Pf6N2E2_2400at 2839.879 kb on + strand, within Pf6N2E2_2400at 2839.879 kb on + strand, within Pf6N2E2_2400at 2839.879 kb on + strand, within Pf6N2E2_2400at 2839.879 kb on + strand, within Pf6N2E2_2400at 2839.880 kb on - strand, within Pf6N2E2_2400at 2839.966 kb on + strand, within Pf6N2E2_2400at 2839.966 kb on + strand, within Pf6N2E2_2400at 2840.095 kb on - strand, within Pf6N2E2_2400at 2840.192 kb on + strand, within Pf6N2E2_2400at 2840.193 kb on - strand, within Pf6N2E2_2400at 2840.357 kb on - strandat 2840.491 kb on + strandat 2840.579 kb on + strandat 2840.580 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,838,874 - Pf6N2E2_2399 0.15 -2.4
2,838,874 - Pf6N2E2_2399 0.15 -2.7
2,839,133 + Pf6N2E2_2399 0.44 -0.1
2,839,133 + Pf6N2E2_2399 0.44 -1.8
2,839,233 + Pf6N2E2_2399 0.56 -0.7
2,839,234 - Pf6N2E2_2399 0.56 -2.1
2,839,234 - Pf6N2E2_2399 0.56 -2.9
2,839,234 - Pf6N2E2_2399 0.56 -2.0
2,839,234 - Pf6N2E2_2399 0.56 -0.0
2,839,234 - Pf6N2E2_2399 0.56 -1.1
2,839,358 + Pf6N2E2_2399 0.70 +0.3
2,839,358 + Pf6N2E2_2399 0.70 +0.0
2,839,359 - Pf6N2E2_2399 0.70 -3.0
2,839,359 - Pf6N2E2_2399 0.70 -2.4
2,839,359 - Pf6N2E2_2399 0.70 -2.3
2,839,359 - Pf6N2E2_2399 0.70 -3.1
2,839,460 + Pf6N2E2_2399 0.82 -2.7
2,839,460 + Pf6N2E2_2399 0.82 -1.5
2,839,461 - Pf6N2E2_2399 0.82 -2.9
2,839,461 - Pf6N2E2_2399 0.82 -1.6
2,839,762 + Pf6N2E2_2400 0.22 +1.1
2,839,763 - Pf6N2E2_2400 0.22 -1.6
2,839,786 + Pf6N2E2_2400 0.25 +0.3
2,839,787 - Pf6N2E2_2400 0.26 +0.9
2,839,829 - Pf6N2E2_2400 0.31 +0.4
2,839,879 + Pf6N2E2_2400 0.38 +0.3
2,839,879 + Pf6N2E2_2400 0.38 -0.2
2,839,879 + Pf6N2E2_2400 0.38 -2.2
2,839,879 + Pf6N2E2_2400 0.38 +1.2
2,839,880 - Pf6N2E2_2400 0.38 -1.5
2,839,966 + Pf6N2E2_2400 0.49 -0.2
2,839,966 + Pf6N2E2_2400 0.49 +0.5
2,840,095 - Pf6N2E2_2400 0.66 -2.0
2,840,192 + Pf6N2E2_2400 0.78 +0.3
2,840,193 - Pf6N2E2_2400 0.78 -1.5
2,840,357 - +0.7
2,840,491 + +1.2
2,840,579 + -0.5
2,840,580 - -0.6

Or see this region's nucleotide sequence