Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2398 and Pf6N2E2_2399 overlap by 1 nucleotides Pf6N2E2_2399 and Pf6N2E2_2400 overlap by 25 nucleotides Pf6N2E2_2400 and Pf6N2E2_2401 are separated by 152 nucleotides
Pf6N2E2_2398 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 2,837,019 to 2,838,746
_2398
Pf6N2E2_2399 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 2,838,746 to 2,839,615
_2399
Pf6N2E2_2400 - Thiol:disulfide interchange protein DsbG precursor, at 2,839,591 to 2,840,358
_2400
Pf6N2E2_2401 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 2,840,511 to 2,841,323
_2401
Position (kb)
2838
2839
2840 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2838.874 kb on - strand, within Pf6N2E2_2399 at 2838.874 kb on - strand, within Pf6N2E2_2399 at 2839.133 kb on + strand, within Pf6N2E2_2399 at 2839.133 kb on + strand, within Pf6N2E2_2399 at 2839.233 kb on + strand, within Pf6N2E2_2399 at 2839.234 kb on - strand, within Pf6N2E2_2399 at 2839.234 kb on - strand, within Pf6N2E2_2399 at 2839.234 kb on - strand, within Pf6N2E2_2399 at 2839.234 kb on - strand, within Pf6N2E2_2399 at 2839.234 kb on - strand, within Pf6N2E2_2399 at 2839.358 kb on + strand, within Pf6N2E2_2399 at 2839.358 kb on + strand, within Pf6N2E2_2399 at 2839.359 kb on - strand, within Pf6N2E2_2399 at 2839.359 kb on - strand, within Pf6N2E2_2399 at 2839.359 kb on - strand, within Pf6N2E2_2399 at 2839.359 kb on - strand, within Pf6N2E2_2399 at 2839.460 kb on + strand, within Pf6N2E2_2399 at 2839.460 kb on + strand, within Pf6N2E2_2399 at 2839.461 kb on - strand, within Pf6N2E2_2399 at 2839.461 kb on - strand, within Pf6N2E2_2399 at 2839.762 kb on + strand, within Pf6N2E2_2400 at 2839.763 kb on - strand, within Pf6N2E2_2400 at 2839.786 kb on + strand, within Pf6N2E2_2400 at 2839.787 kb on - strand, within Pf6N2E2_2400 at 2839.829 kb on - strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.879 kb on + strand, within Pf6N2E2_2400 at 2839.880 kb on - strand, within Pf6N2E2_2400 at 2839.966 kb on + strand, within Pf6N2E2_2400 at 2839.966 kb on + strand, within Pf6N2E2_2400 at 2840.095 kb on - strand, within Pf6N2E2_2400 at 2840.192 kb on + strand, within Pf6N2E2_2400 at 2840.193 kb on - strand, within Pf6N2E2_2400 at 2840.357 kb on - strand at 2840.491 kb on + strand at 2840.579 kb on + strand at 2840.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,838,874 - Pf6N2E2_2399 0.15 -2.4 2,838,874 - Pf6N2E2_2399 0.15 -2.7 2,839,133 + Pf6N2E2_2399 0.44 -0.1 2,839,133 + Pf6N2E2_2399 0.44 -1.8 2,839,233 + Pf6N2E2_2399 0.56 -0.7 2,839,234 - Pf6N2E2_2399 0.56 -2.1 2,839,234 - Pf6N2E2_2399 0.56 -2.9 2,839,234 - Pf6N2E2_2399 0.56 -2.0 2,839,234 - Pf6N2E2_2399 0.56 -0.0 2,839,234 - Pf6N2E2_2399 0.56 -1.1 2,839,358 + Pf6N2E2_2399 0.70 +0.3 2,839,358 + Pf6N2E2_2399 0.70 +0.0 2,839,359 - Pf6N2E2_2399 0.70 -3.0 2,839,359 - Pf6N2E2_2399 0.70 -2.4 2,839,359 - Pf6N2E2_2399 0.70 -2.3 2,839,359 - Pf6N2E2_2399 0.70 -3.1 2,839,460 + Pf6N2E2_2399 0.82 -2.7 2,839,460 + Pf6N2E2_2399 0.82 -1.5 2,839,461 - Pf6N2E2_2399 0.82 -2.9 2,839,461 - Pf6N2E2_2399 0.82 -1.6 2,839,762 + Pf6N2E2_2400 0.22 +1.1 2,839,763 - Pf6N2E2_2400 0.22 -1.6 2,839,786 + Pf6N2E2_2400 0.25 +0.3 2,839,787 - Pf6N2E2_2400 0.26 +0.9 2,839,829 - Pf6N2E2_2400 0.31 +0.4 2,839,879 + Pf6N2E2_2400 0.38 +0.3 2,839,879 + Pf6N2E2_2400 0.38 -0.2 2,839,879 + Pf6N2E2_2400 0.38 -2.2 2,839,879 + Pf6N2E2_2400 0.38 +1.2 2,839,880 - Pf6N2E2_2400 0.38 -1.5 2,839,966 + Pf6N2E2_2400 0.49 -0.2 2,839,966 + Pf6N2E2_2400 0.49 +0.5 2,840,095 - Pf6N2E2_2400 0.66 -2.0 2,840,192 + Pf6N2E2_2400 0.78 +0.3 2,840,193 - Pf6N2E2_2400 0.78 -1.5 2,840,357 - +0.7 2,840,491 + +1.2 2,840,579 + -0.5 2,840,580 - -0.6
Or see this region's nucleotide sequence