Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_235

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_233 and Pf6N2E2_234 are separated by 229 nucleotidesPf6N2E2_234 and Pf6N2E2_235 are separated by 181 nucleotidesPf6N2E2_235 and Pf6N2E2_236 are separated by 177 nucleotides Pf6N2E2_233 - Transcriptional regulator, TetR family, at 296,276 to 296,896 _233 Pf6N2E2_234 - FIG00454312: hypothetical protein, at 297,126 to 297,500 _234 Pf6N2E2_235 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 297,682 to 298,422 _235 Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253 _236 Position (kb) 297 298 299Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.691 kb on + strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.692 kb on - strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.733 kb on + strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.734 kb on - strand, within Pf6N2E2_233at 296.738 kb on - strand, within Pf6N2E2_233at 296.792 kb on + strand, within Pf6N2E2_233at 296.792 kb on + strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.793 kb on - strand, within Pf6N2E2_233at 296.806 kb on + strand, within Pf6N2E2_233at 296.833 kb on + strand, within Pf6N2E2_233at 296.833 kb on + strand, within Pf6N2E2_233at 296.834 kb on - strand, within Pf6N2E2_233at 296.926 kb on + strandat 296.926 kb on + strandat 296.927 kb on - strandat 296.927 kb on - strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.081 kb on + strandat 297.114 kb on - strandat 297.114 kb on - strandat 297.114 kb on - strandat 297.135 kb on + strandat 297.254 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.342 kb on + strand, within Pf6N2E2_234at 297.464 kb on + strandat 297.499 kb on - strandat 297.663 kb on - strandat 297.812 kb on - strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.820 kb on + strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.821 kb on - strand, within Pf6N2E2_235at 297.853 kb on + strand, within Pf6N2E2_235at 297.854 kb on - strand, within Pf6N2E2_235at 297.991 kb on + strand, within Pf6N2E2_235at 297.991 kb on + strand, within Pf6N2E2_235at 297.991 kb on + strand, within Pf6N2E2_235at 297.991 kb on + strand, within Pf6N2E2_235at 298.023 kb on + strand, within Pf6N2E2_235at 298.024 kb on - strand, within Pf6N2E2_235at 298.024 kb on - strand, within Pf6N2E2_235at 298.348 kb on + strand, within Pf6N2E2_235at 298.351 kb on + strandat 298.351 kb on + strandat 298.351 kb on + strandat 298.351 kb on + strandat 298.352 kb on - strandat 298.440 kb on + strandat 298.441 kb on - strandat 298.553 kb on - strandat 298.716 kb on + strand, within Pf6N2E2_236at 298.822 kb on + strand, within Pf6N2E2_236at 299.089 kb on + strand, within Pf6N2E2_236at 299.090 kb on - strand, within Pf6N2E2_236at 299.116 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.129 kb on + strand, within Pf6N2E2_236at 299.130 kb on - strand, within Pf6N2E2_236at 299.130 kb on - strand, within Pf6N2E2_236at 299.165 kb on + strand, within Pf6N2E2_236at 299.272 kb on + strandat 299.272 kb on + strandat 299.301 kb on - strandat 299.307 kb on + strandat 299.392 kb on + strandat 299.393 kb on - strandat 299.413 kb on + strandat 299.414 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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296,691 + Pf6N2E2_233 0.67 +0.1
296,691 + Pf6N2E2_233 0.67 -1.5
296,691 + Pf6N2E2_233 0.67 +1.0
296,691 + Pf6N2E2_233 0.67 -0.8
296,691 + Pf6N2E2_233 0.67 -1.7
296,692 - Pf6N2E2_233 0.67 +1.0
296,692 - Pf6N2E2_233 0.67 -3.5
296,692 - Pf6N2E2_233 0.67 +0.0
296,733 + Pf6N2E2_233 0.74 +1.0
296,733 + Pf6N2E2_233 0.74 +0.3
296,733 + Pf6N2E2_233 0.74 -0.6
296,733 + Pf6N2E2_233 0.74 +0.7
296,733 + Pf6N2E2_233 0.74 -0.5
296,733 + Pf6N2E2_233 0.74 -1.8
296,734 - Pf6N2E2_233 0.74 +0.4
296,734 - Pf6N2E2_233 0.74 -1.9
296,734 - Pf6N2E2_233 0.74 +0.3
296,734 - Pf6N2E2_233 0.74 +0.5
296,738 - Pf6N2E2_233 0.74 -0.6
296,792 + Pf6N2E2_233 0.83 -0.5
296,792 + Pf6N2E2_233 0.83 +0.3
296,793 - Pf6N2E2_233 0.83 -2.3
296,793 - Pf6N2E2_233 0.83 -0.9
296,793 - Pf6N2E2_233 0.83 +0.3
296,793 - Pf6N2E2_233 0.83 +0.2
296,793 - Pf6N2E2_233 0.83 -0.3
296,793 - Pf6N2E2_233 0.83 -0.8
296,793 - Pf6N2E2_233 0.83 -1.0
296,806 + Pf6N2E2_233 0.85 -0.4
296,833 + Pf6N2E2_233 0.90 +0.6
296,833 + Pf6N2E2_233 0.90 +0.8
296,834 - Pf6N2E2_233 0.90 -0.3
296,926 + -0.8
296,926 + +0.2
296,927 - +0.2
296,927 - +0.7
297,081 + +0.6
297,081 + +0.7
297,081 + -1.2
297,081 + -0.7
297,114 - +0.2
297,114 - +0.2
297,114 - +0.7
297,135 + -0.4
297,254 + Pf6N2E2_234 0.34 +0.3
297,342 + Pf6N2E2_234 0.58 -0.5
297,342 + Pf6N2E2_234 0.58 -0.3
297,342 + Pf6N2E2_234 0.58 +0.4
297,342 + Pf6N2E2_234 0.58 +0.3
297,464 + +0.2
297,499 - +1.8
297,663 - -2.6
297,812 - Pf6N2E2_235 0.18 -2.2
297,820 + Pf6N2E2_235 0.19 -0.8
297,820 + Pf6N2E2_235 0.19 -3.2
297,820 + Pf6N2E2_235 0.19 -1.0
297,821 - Pf6N2E2_235 0.19 -1.2
297,821 - Pf6N2E2_235 0.19 -0.4
297,821 - Pf6N2E2_235 0.19 +0.4
297,821 - Pf6N2E2_235 0.19 -2.3
297,821 - Pf6N2E2_235 0.19 -0.2
297,821 - Pf6N2E2_235 0.19 +0.2
297,853 + Pf6N2E2_235 0.23 +0.8
297,854 - Pf6N2E2_235 0.23 +0.2
297,991 + Pf6N2E2_235 0.42 -0.1
297,991 + Pf6N2E2_235 0.42 -0.6
297,991 + Pf6N2E2_235 0.42 -1.6
297,991 + Pf6N2E2_235 0.42 +1.2
298,023 + Pf6N2E2_235 0.46 -1.9
298,024 - Pf6N2E2_235 0.46 -0.7
298,024 - Pf6N2E2_235 0.46 -0.5
298,348 + Pf6N2E2_235 0.90 -1.6
298,351 + +0.1
298,351 + -1.7
298,351 + -0.2
298,351 + -0.5
298,352 - +0.4
298,440 + -0.7
298,441 - -0.3
298,553 - -1.5
298,716 + Pf6N2E2_236 0.18 +0.8
298,822 + Pf6N2E2_236 0.34 -0.6
299,089 + Pf6N2E2_236 0.75 -0.3
299,090 - Pf6N2E2_236 0.75 -0.0
299,116 + Pf6N2E2_236 0.79 +0.3
299,129 + Pf6N2E2_236 0.81 -0.9
299,129 + Pf6N2E2_236 0.81 +0.0
299,129 + Pf6N2E2_236 0.81 +1.7
299,130 - Pf6N2E2_236 0.81 +0.1
299,130 - Pf6N2E2_236 0.81 +0.0
299,165 + Pf6N2E2_236 0.86 -0.9
299,272 + +0.3
299,272 + -1.5
299,301 - -0.4
299,307 + -0.7
299,392 + -1.5
299,393 - +1.1
299,413 + +1.7
299,414 - -0.4

Or see this region's nucleotide sequence