Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2308 and Pf6N2E2_2309 are separated by 395 nucleotides Pf6N2E2_2309 and Pf6N2E2_2310 overlap by 117 nucleotides Pf6N2E2_2310 and Pf6N2E2_2311 overlap by 1 nucleotides Pf6N2E2_2311 and Pf6N2E2_2312 are separated by 18 nucleotides Pf6N2E2_2312 and Pf6N2E2_2313 overlap by 4 nucleotides
Pf6N2E2_2308 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,732,776 to 2,733,732
_2308
Pf6N2E2_2309 - hypothetical protein, at 2,734,128 to 2,734,358
_2309
Pf6N2E2_2310 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 2,734,242 to 2,735,684
_2310
Pf6N2E2_2311 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 2,735,684 to 2,736,292
_2311
Pf6N2E2_2312 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 2,736,311 to 2,736,484
_2312
Pf6N2E2_2313 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,736,481 to 2,737,464
_2313
Position (kb)
2734
2735
2736 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2733.350 kb on + strand, within Pf6N2E2_2308 at 2733.385 kb on + strand, within Pf6N2E2_2308 at 2733.386 kb on - strand, within Pf6N2E2_2308 at 2733.386 kb on - strand, within Pf6N2E2_2308 at 2733.390 kb on - strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.554 kb on - strand, within Pf6N2E2_2308 at 2733.609 kb on + strand, within Pf6N2E2_2308 at 2733.610 kb on - strand, within Pf6N2E2_2308 at 2733.610 kb on - strand, within Pf6N2E2_2308 at 2733.633 kb on - strand, within Pf6N2E2_2308 at 2733.664 kb on + strand at 2733.665 kb on - strand at 2733.670 kb on + strand at 2733.671 kb on - strand at 2733.730 kb on + strand at 2733.731 kb on - strand at 2733.873 kb on + strand at 2733.873 kb on + strand at 2733.873 kb on + strand at 2733.874 kb on - strand at 2733.874 kb on - strand at 2734.034 kb on - strand at 2734.212 kb on - strand, within Pf6N2E2_2309 at 2734.214 kb on + strand, within Pf6N2E2_2309 at 2734.239 kb on - strand, within Pf6N2E2_2309 at 2734.239 kb on - strand, within Pf6N2E2_2309 at 2734.270 kb on - strand at 2734.271 kb on + strand at 2734.271 kb on + strand at 2734.271 kb on + strand at 2734.271 kb on + strand at 2734.271 kb on + strand at 2734.272 kb on - strand at 2734.272 kb on - strand at 2734.275 kb on + strand at 2734.275 kb on + strand at 2734.275 kb on + strand at 2734.275 kb on + strand at 2734.275 kb on + strand at 2734.276 kb on - strand at 2734.276 kb on - strand at 2734.276 kb on - strand at 2734.276 kb on - strand at 2734.276 kb on - strand at 2734.278 kb on - strand at 2734.278 kb on - strand at 2734.283 kb on - strand at 2734.283 kb on - strand at 2734.283 kb on - strand at 2734.476 kb on + strand, within Pf6N2E2_2310 at 2734.476 kb on + strand, within Pf6N2E2_2310 at 2734.477 kb on - strand, within Pf6N2E2_2310 at 2734.477 kb on - strand, within Pf6N2E2_2310 at 2734.480 kb on - strand, within Pf6N2E2_2310 at 2734.480 kb on - strand, within Pf6N2E2_2310 at 2734.480 kb on - strand, within Pf6N2E2_2310 at 2734.480 kb on - strand, within Pf6N2E2_2310 at 2734.480 kb on - strand, within Pf6N2E2_2310 at 2734.594 kb on - strand, within Pf6N2E2_2310 at 2734.612 kb on - strand, within Pf6N2E2_2310 at 2734.665 kb on + strand, within Pf6N2E2_2310 at 2734.666 kb on - strand, within Pf6N2E2_2310 at 2734.666 kb on - strand at 2734.716 kb on + strand, within Pf6N2E2_2310 at 2734.716 kb on + strand, within Pf6N2E2_2310 at 2734.716 kb on + strand, within Pf6N2E2_2310 at 2734.717 kb on - strand, within Pf6N2E2_2310 at 2734.717 kb on - strand, within Pf6N2E2_2310 at 2734.724 kb on + strand, within Pf6N2E2_2310 at 2734.735 kb on - strand, within Pf6N2E2_2310 at 2734.735 kb on - strand, within Pf6N2E2_2310 at 2734.735 kb on - strand, within Pf6N2E2_2310 at 2734.736 kb on - strand, within Pf6N2E2_2310 at 2734.821 kb on + strand, within Pf6N2E2_2310 at 2734.911 kb on + strand, within Pf6N2E2_2310 at 2734.912 kb on - strand, within Pf6N2E2_2310 at 2734.912 kb on - strand, within Pf6N2E2_2310 at 2734.912 kb on - strand, within Pf6N2E2_2310 at 2734.912 kb on - strand, within Pf6N2E2_2310 at 2734.914 kb on + strand, within Pf6N2E2_2310 at 2734.914 kb on + strand, within Pf6N2E2_2310 at 2734.914 kb on + strand, within Pf6N2E2_2310 at 2734.947 kb on + strand, within Pf6N2E2_2310 at 2734.947 kb on + strand, within Pf6N2E2_2310 at 2734.947 kb on + strand, within Pf6N2E2_2310 at 2734.947 kb on + strand, within Pf6N2E2_2310 at 2734.948 kb on - strand, within Pf6N2E2_2310 at 2734.954 kb on - strand, within Pf6N2E2_2310 at 2734.995 kb on + strand, within Pf6N2E2_2310 at 2734.995 kb on + strand, within Pf6N2E2_2310 at 2734.996 kb on - strand, within Pf6N2E2_2310 at 2735.141 kb on + strand, within Pf6N2E2_2310 at 2735.142 kb on - strand, within Pf6N2E2_2310 at 2735.332 kb on - strand, within Pf6N2E2_2310 at 2735.332 kb on - strand, within Pf6N2E2_2310 at 2735.428 kb on - strand, within Pf6N2E2_2310 at 2735.428 kb on - strand, within Pf6N2E2_2310 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.506 kb on - strand, within Pf6N2E2_2310 at 2735.601 kb on + strand at 2735.601 kb on + strand at 2735.602 kb on - strand at 2735.602 kb on - strand at 2735.611 kb on - strand at 2735.669 kb on - strand at 2735.701 kb on - strand at 2735.797 kb on + strand, within Pf6N2E2_2311 at 2735.798 kb on - strand, within Pf6N2E2_2311 at 2735.858 kb on + strand at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.937 kb on - strand, within Pf6N2E2_2311 at 2735.992 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.057 kb on - strand, within Pf6N2E2_2311 at 2736.179 kb on + strand, within Pf6N2E2_2311 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.628 kb on + strand, within Pf6N2E2_2313
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,733,350 + Pf6N2E2_2308 0.60 -1.4 2,733,385 + Pf6N2E2_2308 0.64 -2.6 2,733,386 - Pf6N2E2_2308 0.64 -1.7 2,733,386 - Pf6N2E2_2308 0.64 -2.6 2,733,390 - Pf6N2E2_2308 0.64 -0.5 2,733,553 + Pf6N2E2_2308 0.81 +0.5 2,733,553 + Pf6N2E2_2308 0.81 -0.9 2,733,553 + Pf6N2E2_2308 0.81 -0.6 2,733,554 - Pf6N2E2_2308 0.81 -1.7 2,733,609 + Pf6N2E2_2308 0.87 -0.4 2,733,610 - Pf6N2E2_2308 0.87 -1.7 2,733,610 - Pf6N2E2_2308 0.87 -1.3 2,733,633 - Pf6N2E2_2308 0.90 -0.2 2,733,664 + -2.2 2,733,665 - -1.3 2,733,670 + -2.3 2,733,671 - -0.5 2,733,730 + +0.6 2,733,731 - -0.6 2,733,873 + -1.5 2,733,873 + -0.8 2,733,873 + +0.3 2,733,874 - +1.1 2,733,874 - -2.1 2,734,034 - -0.7 2,734,212 - Pf6N2E2_2309 0.36 -3.2 2,734,214 + Pf6N2E2_2309 0.37 -0.6 2,734,239 - Pf6N2E2_2309 0.48 -2.2 2,734,239 - Pf6N2E2_2309 0.48 -1.0 2,734,270 - -2.3 2,734,271 + -1.6 2,734,271 + -1.7 2,734,271 + -1.9 2,734,271 + -1.7 2,734,271 + -2.9 2,734,272 - -1.6 2,734,272 - -2.1 2,734,275 + -2.3 2,734,275 + -1.9 2,734,275 + +2.4 2,734,275 + -1.0 2,734,275 + +0.6 2,734,276 - -1.9 2,734,276 - -1.8 2,734,276 - -3.7 2,734,276 - -2.2 2,734,276 - -1.5 2,734,278 - -1.6 2,734,278 - +0.3 2,734,283 - -1.4 2,734,283 - -2.6 2,734,283 - -1.0 2,734,476 + Pf6N2E2_2310 0.16 -1.1 2,734,476 + Pf6N2E2_2310 0.16 -0.7 2,734,477 - Pf6N2E2_2310 0.16 -1.4 2,734,477 - Pf6N2E2_2310 0.16 -2.4 2,734,480 - Pf6N2E2_2310 0.16 -1.5 2,734,480 - Pf6N2E2_2310 0.16 -1.0 2,734,480 - Pf6N2E2_2310 0.16 -1.4 2,734,480 - Pf6N2E2_2310 0.16 -2.3 2,734,480 - Pf6N2E2_2310 0.16 -2.2 2,734,594 - Pf6N2E2_2310 0.24 -1.2 2,734,612 - Pf6N2E2_2310 0.26 -3.0 2,734,665 + Pf6N2E2_2310 0.29 -2.3 2,734,666 - Pf6N2E2_2310 0.29 -1.8 2,734,666 - +1.4 2,734,716 + Pf6N2E2_2310 0.33 -0.4 2,734,716 + Pf6N2E2_2310 0.33 -2.2 2,734,716 + Pf6N2E2_2310 0.33 -2.0 2,734,717 - Pf6N2E2_2310 0.33 -1.8 2,734,717 - Pf6N2E2_2310 0.33 -2.0 2,734,724 + Pf6N2E2_2310 0.33 -2.0 2,734,735 - Pf6N2E2_2310 0.34 -2.2 2,734,735 - Pf6N2E2_2310 0.34 -1.4 2,734,735 - Pf6N2E2_2310 0.34 +0.4 2,734,736 - Pf6N2E2_2310 0.34 -0.8 2,734,821 + Pf6N2E2_2310 0.40 -1.6 2,734,911 + Pf6N2E2_2310 0.46 -1.6 2,734,912 - Pf6N2E2_2310 0.46 -2.6 2,734,912 - Pf6N2E2_2310 0.46 -2.9 2,734,912 - Pf6N2E2_2310 0.46 -0.7 2,734,912 - Pf6N2E2_2310 0.46 -1.5 2,734,914 + Pf6N2E2_2310 0.47 -0.6 2,734,914 + Pf6N2E2_2310 0.47 -1.5 2,734,914 + Pf6N2E2_2310 0.47 -2.5 2,734,947 + Pf6N2E2_2310 0.49 -1.0 2,734,947 + Pf6N2E2_2310 0.49 -1.5 2,734,947 + Pf6N2E2_2310 0.49 -2.5 2,734,947 + Pf6N2E2_2310 0.49 -1.1 2,734,948 - Pf6N2E2_2310 0.49 -0.3 2,734,954 - Pf6N2E2_2310 0.49 -1.9 2,734,995 + Pf6N2E2_2310 0.52 -1.4 2,734,995 + Pf6N2E2_2310 0.52 -1.6 2,734,996 - Pf6N2E2_2310 0.52 -1.0 2,735,141 + Pf6N2E2_2310 0.62 -2.8 2,735,142 - Pf6N2E2_2310 0.62 -0.2 2,735,332 - Pf6N2E2_2310 0.76 -1.6 2,735,332 - Pf6N2E2_2310 0.76 -0.7 2,735,428 - Pf6N2E2_2310 0.82 -0.9 2,735,428 - Pf6N2E2_2310 0.82 -2.0 2,735,505 + Pf6N2E2_2310 0.88 -3.1 2,735,505 + Pf6N2E2_2310 0.88 -0.3 2,735,506 - Pf6N2E2_2310 0.88 -1.8 2,735,601 + -2.5 2,735,601 + -1.6 2,735,602 - -0.8 2,735,602 - -0.8 2,735,611 - -1.3 2,735,669 - -1.3 2,735,701 - -0.5 2,735,797 + Pf6N2E2_2311 0.19 -2.2 2,735,798 - Pf6N2E2_2311 0.19 -1.3 2,735,858 + +1.9 2,735,858 + Pf6N2E2_2311 0.29 -0.6 2,735,858 + Pf6N2E2_2311 0.29 -1.7 2,735,858 + Pf6N2E2_2311 0.29 -2.5 2,735,858 + Pf6N2E2_2311 0.29 -2.2 2,735,858 + Pf6N2E2_2311 0.29 -2.6 2,735,858 + Pf6N2E2_2311 0.29 -1.5 2,735,858 + Pf6N2E2_2311 0.29 -0.9 2,735,858 + Pf6N2E2_2311 0.29 -2.2 2,735,858 + Pf6N2E2_2311 0.29 -1.0 2,735,858 + Pf6N2E2_2311 0.29 -2.8 2,735,858 + Pf6N2E2_2311 0.29 +0.6 2,735,858 + Pf6N2E2_2311 0.29 -1.3 2,735,859 - Pf6N2E2_2311 0.29 -1.3 2,735,859 - Pf6N2E2_2311 0.29 -0.0 2,735,859 - Pf6N2E2_2311 0.29 -2.7 2,735,859 - +2.4 2,735,859 - Pf6N2E2_2311 0.29 -1.1 2,735,859 - Pf6N2E2_2311 0.29 -2.1 2,735,927 + Pf6N2E2_2311 0.40 -1.6 2,735,927 + Pf6N2E2_2311 0.40 -0.7 2,735,937 - Pf6N2E2_2311 0.42 -1.7 2,735,992 + Pf6N2E2_2311 0.51 -1.5 2,736,056 + Pf6N2E2_2311 0.61 -1.7 2,736,056 + Pf6N2E2_2311 0.61 -1.5 2,736,056 + Pf6N2E2_2311 0.61 -1.7 2,736,056 + Pf6N2E2_2311 0.61 +0.2 2,736,056 + Pf6N2E2_2311 0.61 +0.6 2,736,056 + Pf6N2E2_2311 0.61 -2.4 2,736,057 - Pf6N2E2_2311 0.61 -2.9 2,736,179 + Pf6N2E2_2311 0.81 -1.0 2,736,466 + Pf6N2E2_2312 0.89 -3.0 2,736,466 + Pf6N2E2_2312 0.89 -0.3 2,736,466 + Pf6N2E2_2312 0.89 -0.1 2,736,628 + Pf6N2E2_2313 0.15 -1.9
Or see this region's nucleotide sequence