Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2175 and Pf6N2E2_2176 are separated by 2 nucleotides Pf6N2E2_2176 and Pf6N2E2_2177 overlap by 4 nucleotides
Pf6N2E2_2175 - Transcriptional regulator, RpiR family, at 2,580,015 to 2,580,869
_2175
Pf6N2E2_2176 - Amidases related to nicotinamidase, at 2,580,872 to 2,581,516
_2176
Pf6N2E2_2177 - Glutamine synthetase (EC 6.3.1.2), putative, at 2,581,513 to 2,582,868
_2177
Position (kb)
2580
2581
2582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2579.881 kb on + strand at 2579.881 kb on + strand at 2579.881 kb on + strand at 2579.882 kb on - strand at 2579.882 kb on - strand at 2579.923 kb on + strand at 2579.924 kb on - strand at 2579.924 kb on - strand at 2579.942 kb on - strand at 2579.942 kb on - strand at 2579.942 kb on - strand at 2579.951 kb on + strand at 2579.955 kb on + strand at 2579.955 kb on + strand at 2579.956 kb on - strand at 2579.956 kb on - strand at 2579.959 kb on + strand at 2579.960 kb on - strand at 2579.975 kb on + strand at 2579.976 kb on - strand at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.459 kb on + strand, within Pf6N2E2_2175 at 2580.459 kb on + strand, within Pf6N2E2_2175 at 2580.660 kb on + strand, within Pf6N2E2_2175 at 2580.867 kb on + strand at 2580.867 kb on + strand at 2581.008 kb on - strand, within Pf6N2E2_2176 at 2581.277 kb on + strand, within Pf6N2E2_2176 at 2581.412 kb on + strand, within Pf6N2E2_2176 at 2581.413 kb on - strand, within Pf6N2E2_2176 at 2581.457 kb on + strand at 2581.633 kb on + strand at 2581.633 kb on + strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.721 kb on - strand, within Pf6N2E2_2177 at 2581.807 kb on - strand, within Pf6N2E2_2177 at 2582.092 kb on + strand, within Pf6N2E2_2177 at 2582.151 kb on - strand, within Pf6N2E2_2177 at 2582.353 kb on + strand, within Pf6N2E2_2177 at 2582.398 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,579,881 + +6.3 2,579,881 + +5.5 2,579,881 + +5.8 2,579,882 - -3.1 2,579,882 - -2.0 2,579,923 + +5.4 2,579,924 - -0.4 2,579,924 - -1.0 2,579,942 - -0.6 2,579,942 - -1.6 2,579,942 - +0.6 2,579,951 + +4.7 2,579,955 + +5.0 2,579,955 + +4.1 2,579,956 - -2.7 2,579,956 - -2.1 2,579,959 + +4.8 2,579,960 - -1.4 2,579,975 + +5.5 2,579,976 - -1.3 2,580,418 + Pf6N2E2_2175 0.47 +1.4 2,580,418 + Pf6N2E2_2175 0.47 -1.2 2,580,418 + Pf6N2E2_2175 0.47 -0.4 2,580,459 + Pf6N2E2_2175 0.52 +1.4 2,580,459 + Pf6N2E2_2175 0.52 +2.8 2,580,660 + Pf6N2E2_2175 0.75 +3.5 2,580,867 + +0.4 2,580,867 + -0.0 2,581,008 - Pf6N2E2_2176 0.21 -1.0 2,581,277 + Pf6N2E2_2176 0.63 -1.5 2,581,412 + Pf6N2E2_2176 0.84 -2.3 2,581,413 - Pf6N2E2_2176 0.84 -2.3 2,581,457 + -1.1 2,581,633 + -1.1 2,581,633 + -2.7 2,581,634 - -1.7 2,581,634 - -1.0 2,581,634 - -2.0 2,581,721 - Pf6N2E2_2177 0.15 -1.0 2,581,807 - Pf6N2E2_2177 0.22 -2.7 2,582,092 + Pf6N2E2_2177 0.43 -0.5 2,582,151 - Pf6N2E2_2177 0.47 -1.6 2,582,353 + Pf6N2E2_2177 0.62 +0.2 2,582,398 + Pf6N2E2_2177 0.65 -0.7 2,582,434 + Pf6N2E2_2177 0.68 +1.8 2,582,434 + Pf6N2E2_2177 0.68 +0.8 2,582,434 + Pf6N2E2_2177 0.68 +1.2 2,582,435 - Pf6N2E2_2177 0.68 -1.7 2,582,435 - Pf6N2E2_2177 0.68 -1.5 2,582,435 - Pf6N2E2_2177 0.68 -1.3 2,582,435 - Pf6N2E2_2177 0.68 +0.3
Or see this region's nucleotide sequence