Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2031 and Pf6N2E2_2032 overlap by 1 nucleotides Pf6N2E2_2032 and Pf6N2E2_2033 are separated by 8 nucleotides
Pf6N2E2_2031 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,419,744 to 2,420,802
_2031
Pf6N2E2_2032 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 2,420,802 to 2,421,824
_2032
Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425
_2033
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2419.909 kb on - strand, within Pf6N2E2_2031 at 2419.978 kb on + strand, within Pf6N2E2_2031 at 2419.978 kb on + strand, within Pf6N2E2_2031 at 2419.979 kb on - strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.371 kb on + strand, within Pf6N2E2_2031 at 2420.372 kb on - strand, within Pf6N2E2_2031 at 2420.476 kb on + strand, within Pf6N2E2_2031 at 2420.476 kb on + strand, within Pf6N2E2_2031 at 2420.524 kb on + strand, within Pf6N2E2_2031 at 2420.657 kb on - strand, within Pf6N2E2_2031 at 2420.657 kb on - strand, within Pf6N2E2_2031 at 2420.713 kb on - strand at 2420.713 kb on - strand at 2420.755 kb on + strand at 2420.755 kb on + strand at 2420.755 kb on + strand at 2420.756 kb on - strand at 2420.756 kb on - strand at 2420.756 kb on - strand at 2420.756 kb on - strand at 2420.952 kb on + strand, within Pf6N2E2_2032 at 2420.953 kb on - strand, within Pf6N2E2_2032 at 2420.968 kb on - strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.114 kb on - strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.233 kb on + strand, within Pf6N2E2_2032 at 2421.303 kb on + strand, within Pf6N2E2_2032 at 2421.304 kb on - strand, within Pf6N2E2_2032 at 2421.306 kb on + strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.373 kb on - strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.505 kb on - strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.595 kb on - strand, within Pf6N2E2_2032 at 2421.865 kb on - strand at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.590 kb on - strand, within Pf6N2E2_2033 at 2422.721 kb on + strand, within Pf6N2E2_2033 at 2422.722 kb on - strand, within Pf6N2E2_2033 at 2422.741 kb on + strand, within Pf6N2E2_2033 at 2422.742 kb on - strand, within Pf6N2E2_2033
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,419,909 - Pf6N2E2_2031 0.16 -1.4 2,419,978 + Pf6N2E2_2031 0.22 +0.1 2,419,978 + Pf6N2E2_2031 0.22 -0.5 2,419,979 - Pf6N2E2_2031 0.22 -0.9 2,420,029 + Pf6N2E2_2031 0.27 -0.1 2,420,029 + Pf6N2E2_2031 0.27 -0.0 2,420,029 + Pf6N2E2_2031 0.27 -0.3 2,420,030 - Pf6N2E2_2031 0.27 -0.6 2,420,030 - Pf6N2E2_2031 0.27 -0.7 2,420,030 - Pf6N2E2_2031 0.27 -0.2 2,420,144 - Pf6N2E2_2031 0.38 -1.9 2,420,144 - Pf6N2E2_2031 0.38 -2.2 2,420,144 - Pf6N2E2_2031 0.38 -2.2 2,420,371 + Pf6N2E2_2031 0.59 -1.3 2,420,372 - Pf6N2E2_2031 0.59 -1.2 2,420,476 + Pf6N2E2_2031 0.69 -0.2 2,420,476 + Pf6N2E2_2031 0.69 -2.5 2,420,524 + Pf6N2E2_2031 0.74 -2.4 2,420,657 - Pf6N2E2_2031 0.86 -0.2 2,420,657 - Pf6N2E2_2031 0.86 -1.4 2,420,713 - -1.9 2,420,713 - -2.2 2,420,755 + -1.8 2,420,755 + -0.7 2,420,755 + -1.2 2,420,756 - -1.8 2,420,756 - +0.6 2,420,756 - -1.3 2,420,756 - -1.8 2,420,952 + Pf6N2E2_2032 0.15 -1.0 2,420,953 - Pf6N2E2_2032 0.15 -1.1 2,420,968 - Pf6N2E2_2032 0.16 -1.0 2,421,045 + Pf6N2E2_2032 0.24 -1.0 2,421,045 + Pf6N2E2_2032 0.24 -0.1 2,421,046 - Pf6N2E2_2032 0.24 +0.7 2,421,046 - Pf6N2E2_2032 0.24 -1.0 2,421,046 - Pf6N2E2_2032 0.24 +0.4 2,421,046 - Pf6N2E2_2032 0.24 -1.8 2,421,046 - Pf6N2E2_2032 0.24 -2.2 2,421,113 + Pf6N2E2_2032 0.30 +0.5 2,421,113 + Pf6N2E2_2032 0.30 -1.1 2,421,114 - Pf6N2E2_2032 0.30 +0.5 2,421,123 + Pf6N2E2_2032 0.31 -1.6 2,421,123 + Pf6N2E2_2032 0.31 -0.9 2,421,123 + Pf6N2E2_2032 0.31 -1.1 2,421,124 - Pf6N2E2_2032 0.31 -2.4 2,421,124 - Pf6N2E2_2032 0.31 -2.6 2,421,124 - Pf6N2E2_2032 0.31 -0.4 2,421,124 - Pf6N2E2_2032 0.31 -1.9 2,421,233 + Pf6N2E2_2032 0.42 +0.1 2,421,303 + Pf6N2E2_2032 0.49 -3.1 2,421,304 - Pf6N2E2_2032 0.49 -2.4 2,421,306 + Pf6N2E2_2032 0.49 +0.7 2,421,307 - Pf6N2E2_2032 0.49 -1.4 2,421,307 - Pf6N2E2_2032 0.49 -1.3 2,421,372 + Pf6N2E2_2032 0.56 -3.4 2,421,372 + Pf6N2E2_2032 0.56 -1.2 2,421,373 - Pf6N2E2_2032 0.56 -3.3 2,421,504 + Pf6N2E2_2032 0.69 -1.3 2,421,504 + Pf6N2E2_2032 0.69 +0.2 2,421,504 + Pf6N2E2_2032 0.69 -1.1 2,421,504 + Pf6N2E2_2032 0.69 -1.9 2,421,505 - Pf6N2E2_2032 0.69 -0.3 2,421,594 + Pf6N2E2_2032 0.77 -0.4 2,421,594 + Pf6N2E2_2032 0.77 +0.2 2,421,594 + Pf6N2E2_2032 0.77 -1.4 2,421,595 - Pf6N2E2_2032 0.78 -2.2 2,421,865 - -0.9 2,422,589 + Pf6N2E2_2033 0.47 -2.0 2,422,589 + Pf6N2E2_2033 0.47 -1.8 2,422,590 - Pf6N2E2_2033 0.48 -2.0 2,422,721 + Pf6N2E2_2033 0.56 -2.8 2,422,722 - Pf6N2E2_2033 0.56 +0.4 2,422,741 + Pf6N2E2_2033 0.57 -2.3 2,422,742 - Pf6N2E2_2033 0.57 -1.1
Or see this region's nucleotide sequence