Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2028 and Pf6N2E2_2029 are separated by 95 nucleotides Pf6N2E2_2029 and Pf6N2E2_2030 are separated by 126 nucleotides Pf6N2E2_2030 and Pf6N2E2_2031 overlap by 1 nucleotides
Pf6N2E2_2028 - Lipase precursor (EC 3.1.1.3), at 2,416,503 to 2,417,372
_2028
Pf6N2E2_2029 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 2,417,468 to 2,417,749
_2029
Pf6N2E2_2030 - ABC transporter, periplasmic substrate-binding protein, at 2,417,876 to 2,419,744
_2030
Pf6N2E2_2031 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,419,744 to 2,420,802
_2031
Position (kb)
2417
2418
2419
2420 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2417.091 kb on + strand, within Pf6N2E2_2028 at 2417.092 kb on - strand, within Pf6N2E2_2028 at 2417.092 kb on - strand, within Pf6N2E2_2028 at 2417.094 kb on + strand, within Pf6N2E2_2028 at 2417.094 kb on + strand, within Pf6N2E2_2028 at 2417.094 kb on + strand, within Pf6N2E2_2028 at 2417.095 kb on - strand, within Pf6N2E2_2028 at 2417.108 kb on + strand, within Pf6N2E2_2028 at 2417.109 kb on - strand, within Pf6N2E2_2028 at 2417.175 kb on + strand, within Pf6N2E2_2028 at 2417.176 kb on - strand, within Pf6N2E2_2028 at 2417.176 kb on - strand, within Pf6N2E2_2028 at 2417.176 kb on - strand, within Pf6N2E2_2028 at 2417.257 kb on - strand, within Pf6N2E2_2028 at 2417.270 kb on + strand, within Pf6N2E2_2028 at 2417.444 kb on - strand at 2417.675 kb on - strand, within Pf6N2E2_2029 at 2417.837 kb on - strand at 2417.902 kb on - strand at 2417.902 kb on - strand at 2417.972 kb on + strand at 2417.973 kb on - strand at 2417.991 kb on - strand at 2418.017 kb on + strand at 2418.017 kb on + strand at 2418.098 kb on + strand, within Pf6N2E2_2030 at 2418.098 kb on + strand, within Pf6N2E2_2030 at 2418.099 kb on - strand, within Pf6N2E2_2030 at 2418.143 kb on + strand, within Pf6N2E2_2030 at 2418.179 kb on + strand, within Pf6N2E2_2030 at 2418.180 kb on - strand, within Pf6N2E2_2030 at 2418.180 kb on - strand, within Pf6N2E2_2030 at 2418.180 kb on - strand, within Pf6N2E2_2030 at 2418.188 kb on + strand, within Pf6N2E2_2030 at 2418.189 kb on - strand, within Pf6N2E2_2030 at 2418.245 kb on + strand, within Pf6N2E2_2030 at 2418.245 kb on + strand, within Pf6N2E2_2030 at 2418.245 kb on + strand, within Pf6N2E2_2030 at 2418.246 kb on - strand, within Pf6N2E2_2030 at 2418.574 kb on + strand, within Pf6N2E2_2030 at 2418.574 kb on + strand, within Pf6N2E2_2030 at 2418.574 kb on + strand, within Pf6N2E2_2030 at 2418.574 kb on + strand, within Pf6N2E2_2030 at 2418.575 kb on - strand, within Pf6N2E2_2030 at 2418.575 kb on - strand, within Pf6N2E2_2030 at 2418.586 kb on + strand, within Pf6N2E2_2030 at 2418.586 kb on + strand, within Pf6N2E2_2030 at 2418.586 kb on + strand, within Pf6N2E2_2030 at 2418.587 kb on - strand, within Pf6N2E2_2030 at 2418.707 kb on + strand, within Pf6N2E2_2030 at 2418.708 kb on - strand, within Pf6N2E2_2030 at 2418.713 kb on + strand, within Pf6N2E2_2030 at 2418.713 kb on + strand, within Pf6N2E2_2030 at 2418.713 kb on + strand, within Pf6N2E2_2030 at 2418.938 kb on + strand, within Pf6N2E2_2030 at 2418.938 kb on + strand, within Pf6N2E2_2030 at 2418.939 kb on + strand, within Pf6N2E2_2030 at 2418.939 kb on - strand, within Pf6N2E2_2030 at 2418.939 kb on - strand, within Pf6N2E2_2030 at 2418.939 kb on - strand, within Pf6N2E2_2030 at 2418.939 kb on - strand, within Pf6N2E2_2030 at 2418.939 kb on - strand, within Pf6N2E2_2030 at 2418.957 kb on - strand, within Pf6N2E2_2030 at 2418.957 kb on - strand, within Pf6N2E2_2030 at 2419.017 kb on + strand, within Pf6N2E2_2030 at 2419.018 kb on - strand, within Pf6N2E2_2030 at 2419.018 kb on - strand, within Pf6N2E2_2030 at 2419.245 kb on - strand, within Pf6N2E2_2030 at 2419.245 kb on - strand, within Pf6N2E2_2030 at 2419.253 kb on - strand, within Pf6N2E2_2030 at 2419.308 kb on - strand, within Pf6N2E2_2030 at 2419.308 kb on - strand, within Pf6N2E2_2030 at 2419.308 kb on - strand, within Pf6N2E2_2030 at 2419.388 kb on + strand, within Pf6N2E2_2030 at 2419.388 kb on + strand, within Pf6N2E2_2030 at 2419.394 kb on + strand, within Pf6N2E2_2030 at 2419.395 kb on - strand, within Pf6N2E2_2030 at 2419.395 kb on - strand, within Pf6N2E2_2030 at 2419.395 kb on - strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.436 kb on + strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.437 kb on - strand, within Pf6N2E2_2030 at 2419.553 kb on + strand, within Pf6N2E2_2030 at 2419.554 kb on - strand, within Pf6N2E2_2030 at 2419.554 kb on - strand, within Pf6N2E2_2030 at 2419.556 kb on + strand, within Pf6N2E2_2030 at 2419.571 kb on + strand at 2419.571 kb on + strand at 2419.571 kb on + strand at 2419.572 kb on - strand at 2419.572 kb on - strand at 2419.572 kb on - strand at 2419.572 kb on - strand at 2419.572 kb on - strand at 2419.572 kb on - strand at 2419.626 kb on + strand at 2419.626 kb on + strand at 2419.753 kb on + strand at 2419.753 kb on + strand at 2419.753 kb on + strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.754 kb on - strand at 2419.755 kb on + strand at 2419.756 kb on - strand at 2419.756 kb on - strand at 2419.756 kb on - strand at 2419.798 kb on - strand at 2419.909 kb on - strand, within Pf6N2E2_2031 at 2419.978 kb on + strand, within Pf6N2E2_2031 at 2419.978 kb on + strand, within Pf6N2E2_2031 at 2419.979 kb on - strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.029 kb on + strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.030 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.144 kb on - strand, within Pf6N2E2_2031 at 2420.371 kb on + strand, within Pf6N2E2_2031 at 2420.372 kb on - strand, within Pf6N2E2_2031 at 2420.476 kb on + strand, within Pf6N2E2_2031 at 2420.476 kb on + strand, within Pf6N2E2_2031 at 2420.524 kb on + strand, within Pf6N2E2_2031 at 2420.657 kb on - strand, within Pf6N2E2_2031 at 2420.657 kb on - strand, within Pf6N2E2_2031 at 2420.713 kb on - strand at 2420.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,417,091 + Pf6N2E2_2028 0.68 +1.5 2,417,092 - Pf6N2E2_2028 0.68 -0.6 2,417,092 - Pf6N2E2_2028 0.68 +1.5 2,417,094 + Pf6N2E2_2028 0.68 -1.2 2,417,094 + Pf6N2E2_2028 0.68 -1.0 2,417,094 + Pf6N2E2_2028 0.68 +0.1 2,417,095 - Pf6N2E2_2028 0.68 -1.2 2,417,108 + Pf6N2E2_2028 0.70 +0.5 2,417,109 - Pf6N2E2_2028 0.70 +0.6 2,417,175 + Pf6N2E2_2028 0.77 +1.8 2,417,176 - Pf6N2E2_2028 0.77 -1.8 2,417,176 - Pf6N2E2_2028 0.77 +0.8 2,417,176 - Pf6N2E2_2028 0.77 -1.0 2,417,257 - Pf6N2E2_2028 0.87 +1.4 2,417,270 + Pf6N2E2_2028 0.88 -0.7 2,417,444 - +0.8 2,417,675 - Pf6N2E2_2029 0.73 -1.9 2,417,837 - -1.3 2,417,902 - +0.2 2,417,902 - -1.0 2,417,972 + -2.6 2,417,973 - -0.8 2,417,991 - -0.1 2,418,017 + -1.5 2,418,017 + -0.3 2,418,098 + Pf6N2E2_2030 0.12 -0.3 2,418,098 + Pf6N2E2_2030 0.12 +0.1 2,418,099 - Pf6N2E2_2030 0.12 -0.3 2,418,143 + Pf6N2E2_2030 0.14 -0.2 2,418,179 + Pf6N2E2_2030 0.16 -0.2 2,418,180 - Pf6N2E2_2030 0.16 +1.3 2,418,180 - Pf6N2E2_2030 0.16 -2.3 2,418,180 - Pf6N2E2_2030 0.16 -3.3 2,418,188 + Pf6N2E2_2030 0.17 -0.7 2,418,189 - Pf6N2E2_2030 0.17 -1.8 2,418,245 + Pf6N2E2_2030 0.20 -2.9 2,418,245 + Pf6N2E2_2030 0.20 -1.4 2,418,245 + Pf6N2E2_2030 0.20 +0.1 2,418,246 - Pf6N2E2_2030 0.20 +0.1 2,418,574 + Pf6N2E2_2030 0.37 -1.0 2,418,574 + Pf6N2E2_2030 0.37 -1.2 2,418,574 + Pf6N2E2_2030 0.37 +0.5 2,418,574 + Pf6N2E2_2030 0.37 -2.1 2,418,575 - Pf6N2E2_2030 0.37 -0.7 2,418,575 - Pf6N2E2_2030 0.37 -1.2 2,418,586 + Pf6N2E2_2030 0.38 -0.2 2,418,586 + Pf6N2E2_2030 0.38 +0.2 2,418,586 + Pf6N2E2_2030 0.38 -0.0 2,418,587 - Pf6N2E2_2030 0.38 -0.7 2,418,707 + Pf6N2E2_2030 0.44 -3.0 2,418,708 - Pf6N2E2_2030 0.45 -0.6 2,418,713 + Pf6N2E2_2030 0.45 -0.6 2,418,713 + Pf6N2E2_2030 0.45 -1.3 2,418,713 + Pf6N2E2_2030 0.45 -0.9 2,418,938 + Pf6N2E2_2030 0.57 -2.5 2,418,938 + Pf6N2E2_2030 0.57 -1.7 2,418,939 + Pf6N2E2_2030 0.57 -0.4 2,418,939 - Pf6N2E2_2030 0.57 -2.0 2,418,939 - Pf6N2E2_2030 0.57 +0.3 2,418,939 - Pf6N2E2_2030 0.57 -1.0 2,418,939 - Pf6N2E2_2030 0.57 -1.0 2,418,939 - Pf6N2E2_2030 0.57 -1.5 2,418,957 - Pf6N2E2_2030 0.58 -2.1 2,418,957 - Pf6N2E2_2030 0.58 +1.7 2,419,017 + Pf6N2E2_2030 0.61 -2.8 2,419,018 - Pf6N2E2_2030 0.61 +0.0 2,419,018 - Pf6N2E2_2030 0.61 -0.9 2,419,245 - Pf6N2E2_2030 0.73 -0.1 2,419,245 - Pf6N2E2_2030 0.73 +0.3 2,419,253 - Pf6N2E2_2030 0.74 +0.7 2,419,308 - Pf6N2E2_2030 0.77 -1.0 2,419,308 - Pf6N2E2_2030 0.77 -3.3 2,419,308 - Pf6N2E2_2030 0.77 -0.5 2,419,388 + Pf6N2E2_2030 0.81 -0.4 2,419,388 + Pf6N2E2_2030 0.81 -1.7 2,419,394 + Pf6N2E2_2030 0.81 -0.2 2,419,395 - Pf6N2E2_2030 0.81 -0.7 2,419,395 - Pf6N2E2_2030 0.81 -3.0 2,419,395 - Pf6N2E2_2030 0.81 -1.5 2,419,436 + Pf6N2E2_2030 0.83 -1.5 2,419,436 + Pf6N2E2_2030 0.83 -1.5 2,419,436 + Pf6N2E2_2030 0.83 -0.8 2,419,436 + Pf6N2E2_2030 0.83 +0.3 2,419,436 + Pf6N2E2_2030 0.83 -1.3 2,419,436 + Pf6N2E2_2030 0.83 +1.3 2,419,437 - Pf6N2E2_2030 0.84 -2.0 2,419,437 - Pf6N2E2_2030 0.84 -1.3 2,419,437 - Pf6N2E2_2030 0.84 -2.0 2,419,437 - Pf6N2E2_2030 0.84 +0.8 2,419,437 - Pf6N2E2_2030 0.84 -4.7 2,419,437 - Pf6N2E2_2030 0.84 -0.3 2,419,437 - Pf6N2E2_2030 0.84 -0.6 2,419,437 - Pf6N2E2_2030 0.84 -2.6 2,419,437 - Pf6N2E2_2030 0.84 -1.1 2,419,553 + Pf6N2E2_2030 0.90 -1.5 2,419,554 - Pf6N2E2_2030 0.90 -2.6 2,419,554 - Pf6N2E2_2030 0.90 -0.2 2,419,556 + Pf6N2E2_2030 0.90 -2.8 2,419,571 + +0.3 2,419,571 + +0.2 2,419,571 + -0.5 2,419,572 - -0.7 2,419,572 - -0.5 2,419,572 - +0.4 2,419,572 - -1.8 2,419,572 - +0.4 2,419,572 - -1.3 2,419,626 + -0.6 2,419,626 + -1.9 2,419,753 + +0.5 2,419,753 + +0.5 2,419,753 + -0.1 2,419,754 - -0.3 2,419,754 - -2.7 2,419,754 - +1.3 2,419,754 - -0.5 2,419,754 - +0.3 2,419,754 - +0.0 2,419,755 + +0.0 2,419,756 - -3.8 2,419,756 - +0.3 2,419,756 - +0.3 2,419,798 - -2.4 2,419,909 - Pf6N2E2_2031 0.16 -1.4 2,419,978 + Pf6N2E2_2031 0.22 +0.1 2,419,978 + Pf6N2E2_2031 0.22 -0.5 2,419,979 - Pf6N2E2_2031 0.22 -0.9 2,420,029 + Pf6N2E2_2031 0.27 -0.1 2,420,029 + Pf6N2E2_2031 0.27 -0.0 2,420,029 + Pf6N2E2_2031 0.27 -0.3 2,420,030 - Pf6N2E2_2031 0.27 -0.6 2,420,030 - Pf6N2E2_2031 0.27 -0.7 2,420,030 - Pf6N2E2_2031 0.27 -0.2 2,420,144 - Pf6N2E2_2031 0.38 -1.9 2,420,144 - Pf6N2E2_2031 0.38 -2.2 2,420,144 - Pf6N2E2_2031 0.38 -2.2 2,420,371 + Pf6N2E2_2031 0.59 -1.3 2,420,372 - Pf6N2E2_2031 0.59 -1.2 2,420,476 + Pf6N2E2_2031 0.69 -0.2 2,420,476 + Pf6N2E2_2031 0.69 -2.5 2,420,524 + Pf6N2E2_2031 0.74 -2.4 2,420,657 - Pf6N2E2_2031 0.86 -0.2 2,420,657 - Pf6N2E2_2031 0.86 -1.4 2,420,713 - -1.9 2,420,713 - -2.2
Or see this region's nucleotide sequence