Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1783

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1781 and Pf6N2E2_1782 are separated by 16 nucleotidesPf6N2E2_1782 and Pf6N2E2_1783 are separated by 32 nucleotidesPf6N2E2_1783 and Pf6N2E2_1784 are separated by 11 nucleotides Pf6N2E2_1781 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,106,229 to 2,108,358 _1781 Pf6N2E2_1782 - STAS domain protein, at 2,108,375 to 2,108,692 _1782 Pf6N2E2_1783 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,108,725 to 2,109,093 _1783 Pf6N2E2_1784 - Methyl-accepting chemotaxis protein, at 2,109,105 to 2,110,223 _1784 Position (kb) 2108 2109 2110Strain fitness (log2 ratio) -2 -1 0 1 2at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2108.221 kb on - strandat 2108.339 kb on - strandat 2108.631 kb on + strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.727 kb on - strandat 2108.873 kb on + strand, within Pf6N2E2_1783at 2108.942 kb on - strand, within Pf6N2E2_1783at 2108.942 kb on - strand, within Pf6N2E2_1783at 2109.107 kb on - strandat 2109.131 kb on + strandat 2109.131 kb on + strandat 2109.132 kb on - strandat 2109.132 kb on - strandat 2109.184 kb on + strandat 2109.184 kb on + strandat 2109.185 kb on - strandat 2109.765 kb on + strand, within Pf6N2E2_1784at 2109.766 kb on - strand, within Pf6N2E2_1784at 2110.008 kb on + strand, within Pf6N2E2_1784

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,107,891 - Pf6N2E2_1781 0.78 +0.9
2,107,891 - Pf6N2E2_1781 0.78 +1.2
2,107,891 - Pf6N2E2_1781 0.78 +2.3
2,107,891 - Pf6N2E2_1781 0.78 +1.2
2,108,221 - +0.4
2,108,339 - -0.0
2,108,631 + Pf6N2E2_1782 0.81 -0.1
2,108,632 - Pf6N2E2_1782 0.81 +1.0
2,108,632 - Pf6N2E2_1782 0.81 +1.4
2,108,632 - Pf6N2E2_1782 0.81 -0.2
2,108,727 - -2.3
2,108,873 + Pf6N2E2_1783 0.40 +0.7
2,108,942 - Pf6N2E2_1783 0.59 -0.3
2,108,942 - Pf6N2E2_1783 0.59 -1.9
2,109,107 - -1.6
2,109,131 + +1.2
2,109,131 + -0.5
2,109,132 - -1.7
2,109,132 - -1.0
2,109,184 + +0.2
2,109,184 + -0.2
2,109,185 - -0.3
2,109,765 + Pf6N2E2_1784 0.59 +1.7
2,109,766 - Pf6N2E2_1784 0.59 -1.5
2,110,008 + Pf6N2E2_1784 0.81 +1.8

Or see this region's nucleotide sequence