Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1781

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1780 and Pf6N2E2_1781 are separated by 21 nucleotidesPf6N2E2_1781 and Pf6N2E2_1782 are separated by 16 nucleotidesPf6N2E2_1782 and Pf6N2E2_1783 are separated by 32 nucleotidesPf6N2E2_1783 and Pf6N2E2_1784 are separated by 11 nucleotides Pf6N2E2_1780 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,103,817 to 2,106,207 _1780 Pf6N2E2_1781 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,106,229 to 2,108,358 _1781 Pf6N2E2_1782 - STAS domain protein, at 2,108,375 to 2,108,692 _1782 Pf6N2E2_1783 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,108,725 to 2,109,093 _1783 Pf6N2E2_1784 - Methyl-accepting chemotaxis protein, at 2,109,105 to 2,110,223 _1784 Position (kb) 2106 2107 2108 2109Strain fitness (log2 ratio) -2 -1 0 1 2at 2105.323 kb on - strand, within Pf6N2E2_1780at 2105.323 kb on - strand, within Pf6N2E2_1780at 2105.366 kb on + strand, within Pf6N2E2_1780at 2105.640 kb on + strand, within Pf6N2E2_1780at 2105.641 kb on - strand, within Pf6N2E2_1780at 2105.678 kb on + strand, within Pf6N2E2_1780at 2105.679 kb on - strand, within Pf6N2E2_1780at 2105.679 kb on - strand, within Pf6N2E2_1780at 2105.679 kb on - strand, within Pf6N2E2_1780at 2105.856 kb on + strand, within Pf6N2E2_1780at 2105.868 kb on - strand, within Pf6N2E2_1780at 2106.095 kb on + strandat 2106.096 kb on - strandat 2106.215 kb on + strandat 2106.215 kb on + strandat 2106.215 kb on + strandat 2106.230 kb on + strandat 2106.230 kb on + strandat 2106.230 kb on + strandat 2106.231 kb on - strandat 2106.258 kb on + strandat 2106.259 kb on - strandat 2106.293 kb on - strandat 2106.486 kb on + strand, within Pf6N2E2_1781at 2106.623 kb on + strand, within Pf6N2E2_1781at 2106.623 kb on + strand, within Pf6N2E2_1781at 2106.623 kb on + strand, within Pf6N2E2_1781at 2106.624 kb on - strand, within Pf6N2E2_1781at 2106.624 kb on - strand, within Pf6N2E2_1781at 2106.703 kb on + strand, within Pf6N2E2_1781at 2106.975 kb on - strand, within Pf6N2E2_1781at 2107.404 kb on + strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2107.891 kb on - strand, within Pf6N2E2_1781at 2108.221 kb on - strandat 2108.339 kb on - strandat 2108.631 kb on + strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.632 kb on - strand, within Pf6N2E2_1782at 2108.727 kb on - strandat 2108.873 kb on + strand, within Pf6N2E2_1783at 2108.942 kb on - strand, within Pf6N2E2_1783at 2108.942 kb on - strand, within Pf6N2E2_1783at 2109.107 kb on - strandat 2109.131 kb on + strandat 2109.131 kb on + strandat 2109.132 kb on - strandat 2109.132 kb on - strandat 2109.184 kb on + strandat 2109.184 kb on + strandat 2109.185 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,105,323 - Pf6N2E2_1780 0.63 +0.1
2,105,323 - Pf6N2E2_1780 0.63 +0.2
2,105,366 + Pf6N2E2_1780 0.65 -0.4
2,105,640 + Pf6N2E2_1780 0.76 +0.8
2,105,641 - Pf6N2E2_1780 0.76 -1.2
2,105,678 + Pf6N2E2_1780 0.78 +2.0
2,105,679 - Pf6N2E2_1780 0.78 +0.1
2,105,679 - Pf6N2E2_1780 0.78 +0.9
2,105,679 - Pf6N2E2_1780 0.78 +1.6
2,105,856 + Pf6N2E2_1780 0.85 -0.8
2,105,868 - Pf6N2E2_1780 0.86 -1.2
2,106,095 + +0.8
2,106,096 - -0.6
2,106,215 + -0.7
2,106,215 + +1.0
2,106,215 + +0.6
2,106,230 + -0.3
2,106,230 + -2.6
2,106,230 + +0.6
2,106,231 - -1.8
2,106,258 + -0.6
2,106,259 - +0.1
2,106,293 - +1.2
2,106,486 + Pf6N2E2_1781 0.12 -1.7
2,106,623 + Pf6N2E2_1781 0.18 -2.6
2,106,623 + Pf6N2E2_1781 0.18 +0.7
2,106,623 + Pf6N2E2_1781 0.18 -1.3
2,106,624 - Pf6N2E2_1781 0.19 +0.2
2,106,624 - Pf6N2E2_1781 0.19 +0.9
2,106,703 + Pf6N2E2_1781 0.22 -0.3
2,106,975 - Pf6N2E2_1781 0.35 +2.5
2,107,404 + Pf6N2E2_1781 0.55 +0.1
2,107,891 - Pf6N2E2_1781 0.78 +0.9
2,107,891 - Pf6N2E2_1781 0.78 +1.2
2,107,891 - Pf6N2E2_1781 0.78 +2.3
2,107,891 - Pf6N2E2_1781 0.78 +1.2
2,108,221 - +0.4
2,108,339 - -0.0
2,108,631 + Pf6N2E2_1782 0.81 -0.1
2,108,632 - Pf6N2E2_1782 0.81 +1.0
2,108,632 - Pf6N2E2_1782 0.81 +1.4
2,108,632 - Pf6N2E2_1782 0.81 -0.2
2,108,727 - -2.3
2,108,873 + Pf6N2E2_1783 0.40 +0.7
2,108,942 - Pf6N2E2_1783 0.59 -0.3
2,108,942 - Pf6N2E2_1783 0.59 -1.9
2,109,107 - -1.6
2,109,131 + +1.2
2,109,131 + -0.5
2,109,132 - -1.7
2,109,132 - -1.0
2,109,184 + +0.2
2,109,184 + -0.2
2,109,185 - -0.3

Or see this region's nucleotide sequence