Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1780 and Pf6N2E2_1781 are separated by 21 nucleotides Pf6N2E2_1781 and Pf6N2E2_1782 are separated by 16 nucleotides Pf6N2E2_1782 and Pf6N2E2_1783 are separated by 32 nucleotides Pf6N2E2_1783 and Pf6N2E2_1784 are separated by 11 nucleotides
Pf6N2E2_1780 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,103,817 to 2,106,207
_1780
Pf6N2E2_1781 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,106,229 to 2,108,358
_1781
Pf6N2E2_1782 - STAS domain protein, at 2,108,375 to 2,108,692
_1782
Pf6N2E2_1783 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,108,725 to 2,109,093
_1783
Pf6N2E2_1784 - Methyl-accepting chemotaxis protein, at 2,109,105 to 2,110,223
_1784
Position (kb)
2106
2107
2108
2109 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2105.323 kb on - strand, within Pf6N2E2_1780 at 2105.323 kb on - strand, within Pf6N2E2_1780 at 2105.366 kb on + strand, within Pf6N2E2_1780 at 2105.640 kb on + strand, within Pf6N2E2_1780 at 2105.641 kb on - strand, within Pf6N2E2_1780 at 2105.678 kb on + strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.679 kb on - strand, within Pf6N2E2_1780 at 2105.856 kb on + strand, within Pf6N2E2_1780 at 2105.868 kb on - strand, within Pf6N2E2_1780 at 2106.095 kb on + strand at 2106.096 kb on - strand at 2106.215 kb on + strand at 2106.215 kb on + strand at 2106.215 kb on + strand at 2106.230 kb on + strand at 2106.230 kb on + strand at 2106.230 kb on + strand at 2106.231 kb on - strand at 2106.258 kb on + strand at 2106.259 kb on - strand at 2106.293 kb on - strand at 2106.486 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.623 kb on + strand, within Pf6N2E2_1781 at 2106.624 kb on - strand, within Pf6N2E2_1781 at 2106.624 kb on - strand, within Pf6N2E2_1781 at 2106.703 kb on + strand, within Pf6N2E2_1781 at 2106.975 kb on - strand, within Pf6N2E2_1781 at 2107.404 kb on + strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2107.891 kb on - strand, within Pf6N2E2_1781 at 2108.221 kb on - strand at 2108.339 kb on - strand at 2108.631 kb on + strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.632 kb on - strand, within Pf6N2E2_1782 at 2108.727 kb on - strand at 2108.873 kb on + strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2108.942 kb on - strand, within Pf6N2E2_1783 at 2109.107 kb on - strand at 2109.131 kb on + strand at 2109.131 kb on + strand at 2109.132 kb on - strand at 2109.132 kb on - strand at 2109.184 kb on + strand at 2109.184 kb on + strand at 2109.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,105,323 - Pf6N2E2_1780 0.63 +0.1 2,105,323 - Pf6N2E2_1780 0.63 +0.2 2,105,366 + Pf6N2E2_1780 0.65 -0.4 2,105,640 + Pf6N2E2_1780 0.76 +0.8 2,105,641 - Pf6N2E2_1780 0.76 -1.2 2,105,678 + Pf6N2E2_1780 0.78 +2.0 2,105,679 - Pf6N2E2_1780 0.78 +0.1 2,105,679 - Pf6N2E2_1780 0.78 +0.9 2,105,679 - Pf6N2E2_1780 0.78 +1.6 2,105,856 + Pf6N2E2_1780 0.85 -0.8 2,105,868 - Pf6N2E2_1780 0.86 -1.2 2,106,095 + +0.8 2,106,096 - -0.6 2,106,215 + -0.7 2,106,215 + +1.0 2,106,215 + +0.6 2,106,230 + -0.3 2,106,230 + -2.6 2,106,230 + +0.6 2,106,231 - -1.8 2,106,258 + -0.6 2,106,259 - +0.1 2,106,293 - +1.2 2,106,486 + Pf6N2E2_1781 0.12 -1.7 2,106,623 + Pf6N2E2_1781 0.18 -2.6 2,106,623 + Pf6N2E2_1781 0.18 +0.7 2,106,623 + Pf6N2E2_1781 0.18 -1.3 2,106,624 - Pf6N2E2_1781 0.19 +0.2 2,106,624 - Pf6N2E2_1781 0.19 +0.9 2,106,703 + Pf6N2E2_1781 0.22 -0.3 2,106,975 - Pf6N2E2_1781 0.35 +2.5 2,107,404 + Pf6N2E2_1781 0.55 +0.1 2,107,891 - Pf6N2E2_1781 0.78 +0.9 2,107,891 - Pf6N2E2_1781 0.78 +1.2 2,107,891 - Pf6N2E2_1781 0.78 +2.3 2,107,891 - Pf6N2E2_1781 0.78 +1.2 2,108,221 - +0.4 2,108,339 - -0.0 2,108,631 + Pf6N2E2_1782 0.81 -0.1 2,108,632 - Pf6N2E2_1782 0.81 +1.0 2,108,632 - Pf6N2E2_1782 0.81 +1.4 2,108,632 - Pf6N2E2_1782 0.81 -0.2 2,108,727 - -2.3 2,108,873 + Pf6N2E2_1783 0.40 +0.7 2,108,942 - Pf6N2E2_1783 0.59 -0.3 2,108,942 - Pf6N2E2_1783 0.59 -1.9 2,109,107 - -1.6 2,109,131 + +1.2 2,109,131 + -0.5 2,109,132 - -1.7 2,109,132 - -1.0 2,109,184 + +0.2 2,109,184 + -0.2 2,109,185 - -0.3
Or see this region's nucleotide sequence