Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1778

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1776 and Pf6N2E2_1777 are separated by 145 nucleotidesPf6N2E2_1777 and Pf6N2E2_1778 overlap by 4 nucleotidesPf6N2E2_1778 and Pf6N2E2_1779 are separated by 75 nucleotidesPf6N2E2_1779 and Pf6N2E2_1780 are separated by 3 nucleotides Pf6N2E2_1776 - Chemotaxis protein CheD, at 2,101,387 to 2,101,755 _1776 Pf6N2E2_1777 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 2,101,901 to 2,102,704 _1777 Pf6N2E2_1778 - Positive regulator of CheA protein activity (CheW), at 2,102,701 to 2,103,225 _1778 Pf6N2E2_1779 - Positive regulator of CheA protein activity (CheW), at 2,103,301 to 2,103,813 _1779 Pf6N2E2_1780 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,103,817 to 2,106,207 _1780 Position (kb) 2102 2103 2104Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2101.768 kb on - strandat 2101.827 kb on + strandat 2101.831 kb on + strandat 2101.831 kb on + strandat 2101.831 kb on + strandat 2101.831 kb on + strandat 2101.831 kb on + strandat 2101.831 kb on + strandat 2101.832 kb on - strandat 2101.832 kb on - strandat 2101.832 kb on - strandat 2101.880 kb on + strandat 2101.920 kb on + strandat 2101.924 kb on + strandat 2101.924 kb on + strandat 2101.924 kb on + strandat 2101.925 kb on - strandat 2101.925 kb on - strandat 2101.925 kb on - strandat 2102.030 kb on + strand, within Pf6N2E2_1777at 2102.031 kb on - strand, within Pf6N2E2_1777at 2102.058 kb on + strand, within Pf6N2E2_1777at 2102.058 kb on + strand, within Pf6N2E2_1777at 2102.058 kb on + strand, within Pf6N2E2_1777at 2102.059 kb on - strand, within Pf6N2E2_1777at 2102.059 kb on - strand, within Pf6N2E2_1777at 2102.140 kb on + strand, within Pf6N2E2_1777at 2102.140 kb on + strand, within Pf6N2E2_1777at 2102.208 kb on + strand, within Pf6N2E2_1777at 2102.208 kb on + strand, within Pf6N2E2_1777at 2102.208 kb on + strand, within Pf6N2E2_1777at 2102.209 kb on - strand, within Pf6N2E2_1777at 2102.362 kb on - strand, within Pf6N2E2_1777at 2102.398 kb on + strand, within Pf6N2E2_1777at 2102.398 kb on + strand, within Pf6N2E2_1777at 2102.399 kb on - strand, within Pf6N2E2_1777at 2102.399 kb on - strand, within Pf6N2E2_1777at 2102.399 kb on - strand, within Pf6N2E2_1777at 2102.403 kb on - strand, within Pf6N2E2_1777at 2102.547 kb on + strand, within Pf6N2E2_1777at 2102.550 kb on + strand, within Pf6N2E2_1777at 2102.551 kb on - strand, within Pf6N2E2_1777at 2102.551 kb on - strand, within Pf6N2E2_1777at 2102.551 kb on - strand, within Pf6N2E2_1777at 2102.559 kb on + strand, within Pf6N2E2_1777at 2102.631 kb on - strandat 2102.786 kb on - strand, within Pf6N2E2_1778at 2102.838 kb on + strand, within Pf6N2E2_1778at 2102.838 kb on + strand, within Pf6N2E2_1778at 2102.839 kb on - strand, within Pf6N2E2_1778at 2102.973 kb on + strand, within Pf6N2E2_1778at 2103.105 kb on - strand, within Pf6N2E2_1778at 2103.174 kb on - strandat 2103.174 kb on - strandat 2103.237 kb on - strandat 2103.250 kb on - strandat 2103.279 kb on - strandat 2103.279 kb on - strandat 2103.279 kb on - strandat 2103.637 kb on - strand, within Pf6N2E2_1779at 2103.703 kb on - strand, within Pf6N2E2_1779at 2103.713 kb on + strand, within Pf6N2E2_1779at 2103.714 kb on - strand, within Pf6N2E2_1779at 2103.714 kb on - strand, within Pf6N2E2_1779at 2103.730 kb on + strand, within Pf6N2E2_1779

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,101,768 - +3.2
2,101,827 + +1.0
2,101,831 + -0.5
2,101,831 + -0.7
2,101,831 + +1.0
2,101,831 + +1.7
2,101,831 + -1.1
2,101,831 + +1.8
2,101,832 - +0.6
2,101,832 - +0.4
2,101,832 - -0.8
2,101,880 + +1.2
2,101,920 + +0.8
2,101,924 + -0.5
2,101,924 + +0.6
2,101,924 + +0.7
2,101,925 - +0.2
2,101,925 - +1.0
2,101,925 - +2.0
2,102,030 + Pf6N2E2_1777 0.16 -0.4
2,102,031 - Pf6N2E2_1777 0.16 -0.7
2,102,058 + Pf6N2E2_1777 0.20 +0.0
2,102,058 + Pf6N2E2_1777 0.20 -0.9
2,102,058 + Pf6N2E2_1777 0.20 +0.4
2,102,059 - Pf6N2E2_1777 0.20 +0.3
2,102,059 - Pf6N2E2_1777 0.20 -1.4
2,102,140 + Pf6N2E2_1777 0.30 -1.3
2,102,140 + Pf6N2E2_1777 0.30 -0.5
2,102,208 + Pf6N2E2_1777 0.38 -1.5
2,102,208 + Pf6N2E2_1777 0.38 +0.3
2,102,208 + Pf6N2E2_1777 0.38 +1.6
2,102,209 - Pf6N2E2_1777 0.38 -0.2
2,102,362 - Pf6N2E2_1777 0.57 -1.5
2,102,398 + Pf6N2E2_1777 0.62 -0.2
2,102,398 + Pf6N2E2_1777 0.62 -0.1
2,102,399 - Pf6N2E2_1777 0.62 -1.6
2,102,399 - Pf6N2E2_1777 0.62 +0.1
2,102,399 - Pf6N2E2_1777 0.62 +1.0
2,102,403 - Pf6N2E2_1777 0.62 +0.3
2,102,547 + Pf6N2E2_1777 0.80 -0.1
2,102,550 + Pf6N2E2_1777 0.81 +1.1
2,102,551 - Pf6N2E2_1777 0.81 -0.5
2,102,551 - Pf6N2E2_1777 0.81 -0.1
2,102,551 - Pf6N2E2_1777 0.81 -0.4
2,102,559 + Pf6N2E2_1777 0.82 -0.6
2,102,631 - +0.1
2,102,786 - Pf6N2E2_1778 0.16 -1.0
2,102,838 + Pf6N2E2_1778 0.26 -0.8
2,102,838 + Pf6N2E2_1778 0.26 +2.4
2,102,839 - Pf6N2E2_1778 0.26 +1.0
2,102,973 + Pf6N2E2_1778 0.52 -1.5
2,103,105 - Pf6N2E2_1778 0.77 -2.1
2,103,174 - -0.3
2,103,174 - +0.2
2,103,237 - -2.7
2,103,250 - +0.3
2,103,279 - -0.2
2,103,279 - -0.5
2,103,279 - -1.6
2,103,637 - Pf6N2E2_1779 0.65 -0.2
2,103,703 - Pf6N2E2_1779 0.78 -0.0
2,103,713 + Pf6N2E2_1779 0.80 -1.6
2,103,714 - Pf6N2E2_1779 0.81 +0.9
2,103,714 - Pf6N2E2_1779 0.81 -0.8
2,103,730 + Pf6N2E2_1779 0.84 -1.3

Or see this region's nucleotide sequence