Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1774

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1773 and Pf6N2E2_1774 overlap by 4 nucleotidesPf6N2E2_1774 and Pf6N2E2_1775 are separated by 15 nucleotides Pf6N2E2_1773 - Sensory box histidine kinase, at 2,096,676 to 2,099,672 _1773 Pf6N2E2_1774 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 2,099,669 to 2,100,298 _1774 Pf6N2E2_1775 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 2,100,314 to 2,101,390 _1775 Position (kb) 2099 2100 2101Strain fitness (log2 ratio) -3 -2 -1 0 1at 2098.673 kb on + strand, within Pf6N2E2_1773at 2098.674 kb on - strand, within Pf6N2E2_1773at 2098.674 kb on - strand, within Pf6N2E2_1773at 2098.807 kb on + strand, within Pf6N2E2_1773at 2098.863 kb on + strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.864 kb on - strand, within Pf6N2E2_1773at 2098.970 kb on + strand, within Pf6N2E2_1773at 2098.970 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.972 kb on + strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2098.973 kb on - strand, within Pf6N2E2_1773at 2099.154 kb on + strand, within Pf6N2E2_1773at 2099.396 kb on + strandat 2099.397 kb on - strandat 2099.456 kb on + strandat 2099.821 kb on - strand, within Pf6N2E2_1774at 2100.200 kb on - strand, within Pf6N2E2_1774at 2100.418 kb on - strandat 2100.418 kb on - strandat 2100.418 kb on - strandat 2100.647 kb on + strand, within Pf6N2E2_1775at 2100.648 kb on - strand, within Pf6N2E2_1775at 2100.648 kb on - strand, within Pf6N2E2_1775at 2100.653 kb on - strand, within Pf6N2E2_1775at 2100.831 kb on - strand, within Pf6N2E2_1775at 2100.831 kb on - strand, within Pf6N2E2_1775at 2100.865 kb on + strand, within Pf6N2E2_1775at 2100.866 kb on - strand, within Pf6N2E2_1775at 2101.196 kb on - strand, within Pf6N2E2_1775at 2101.273 kb on - strand, within Pf6N2E2_1775

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,098,673 + Pf6N2E2_1773 0.67 -0.6
2,098,674 - Pf6N2E2_1773 0.67 -2.6
2,098,674 - Pf6N2E2_1773 0.67 -1.8
2,098,807 + Pf6N2E2_1773 0.71 +0.2
2,098,863 + Pf6N2E2_1773 0.73 +1.6
2,098,864 - Pf6N2E2_1773 0.73 -0.7
2,098,864 - Pf6N2E2_1773 0.73 +0.4
2,098,864 - Pf6N2E2_1773 0.73 -0.2
2,098,864 - Pf6N2E2_1773 0.73 +1.7
2,098,864 - Pf6N2E2_1773 0.73 -1.7
2,098,864 - Pf6N2E2_1773 0.73 -2.3
2,098,864 - Pf6N2E2_1773 0.73 +0.6
2,098,864 - Pf6N2E2_1773 0.73 -0.4
2,098,864 - Pf6N2E2_1773 0.73 -1.3
2,098,970 + Pf6N2E2_1773 0.77 +1.5
2,098,970 + Pf6N2E2_1773 0.77 -1.8
2,098,972 + Pf6N2E2_1773 0.77 +1.7
2,098,972 + Pf6N2E2_1773 0.77 +1.5
2,098,972 + Pf6N2E2_1773 0.77 -1.4
2,098,972 + Pf6N2E2_1773 0.77 -1.2
2,098,972 + Pf6N2E2_1773 0.77 -0.4
2,098,972 + Pf6N2E2_1773 0.77 +0.0
2,098,973 - Pf6N2E2_1773 0.77 -1.8
2,098,973 - Pf6N2E2_1773 0.77 +0.8
2,098,973 - Pf6N2E2_1773 0.77 -0.9
2,098,973 - Pf6N2E2_1773 0.77 +0.7
2,098,973 - Pf6N2E2_1773 0.77 +0.6
2,098,973 - Pf6N2E2_1773 0.77 -0.5
2,098,973 - Pf6N2E2_1773 0.77 -0.3
2,099,154 + Pf6N2E2_1773 0.83 -0.3
2,099,396 + +0.7
2,099,397 - -0.8
2,099,456 + -0.2
2,099,821 - Pf6N2E2_1774 0.24 -3.3
2,100,200 - Pf6N2E2_1774 0.84 -0.8
2,100,418 - +0.2
2,100,418 - +0.3
2,100,418 - +0.5
2,100,647 + Pf6N2E2_1775 0.31 +0.5
2,100,648 - Pf6N2E2_1775 0.31 +1.1
2,100,648 - Pf6N2E2_1775 0.31 -1.1
2,100,653 - Pf6N2E2_1775 0.31 -1.2
2,100,831 - Pf6N2E2_1775 0.48 +0.6
2,100,831 - Pf6N2E2_1775 0.48 -0.1
2,100,865 + Pf6N2E2_1775 0.51 -0.3
2,100,866 - Pf6N2E2_1775 0.51 +0.4
2,101,196 - Pf6N2E2_1775 0.82 -0.2
2,101,273 - Pf6N2E2_1775 0.89 +0.2

Or see this region's nucleotide sequence