Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1757

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1755 and Pf6N2E2_1756 are separated by 73 nucleotidesPf6N2E2_1756 and Pf6N2E2_1757 are separated by 106 nucleotidesPf6N2E2_1757 and Pf6N2E2_1758 are separated by 83 nucleotidesPf6N2E2_1758 and Pf6N2E2_1759 are separated by 8 nucleotides Pf6N2E2_1755 - 2-Oxobutyrate oxidase, putative, at 2,073,481 to 2,074,533 _1755 Pf6N2E2_1756 - Arginine ABC transporter, periplasmic arginine-binding protein ArtJ, at 2,074,607 to 2,075,413 _1756 Pf6N2E2_1757 - glutamine ABC transporter ATP-binding component, at 2,075,520 to 2,077,028 _1757 Pf6N2E2_1758 - Transcriptional regulator, MarR family, at 2,077,112 to 2,077,567 _1758 Pf6N2E2_1759 - uncharacterized domain 1, at 2,077,576 to 2,078,187 _1759 Position (kb) 2075 2076 2077 2078Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2074.628 kb on - strandat 2074.628 kb on - strandat 2074.755 kb on - strand, within Pf6N2E2_1756at 2074.887 kb on - strand, within Pf6N2E2_1756at 2074.887 kb on - strand, within Pf6N2E2_1756at 2074.910 kb on + strand, within Pf6N2E2_1756at 2074.911 kb on - strand, within Pf6N2E2_1756at 2074.911 kb on - strand, within Pf6N2E2_1756at 2074.911 kb on - strand, within Pf6N2E2_1756at 2074.911 kb on - strand, within Pf6N2E2_1756at 2074.953 kb on - strand, within Pf6N2E2_1756at 2075.093 kb on + strand, within Pf6N2E2_1756at 2075.230 kb on + strand, within Pf6N2E2_1756at 2075.231 kb on - strand, within Pf6N2E2_1756at 2075.231 kb on - strand, within Pf6N2E2_1756at 2075.270 kb on + strand, within Pf6N2E2_1756at 2075.270 kb on + strand, within Pf6N2E2_1756at 2075.270 kb on + strand, within Pf6N2E2_1756at 2075.271 kb on - strand, within Pf6N2E2_1756at 2075.288 kb on - strand, within Pf6N2E2_1756at 2075.292 kb on - strand, within Pf6N2E2_1756at 2075.318 kb on + strand, within Pf6N2E2_1756at 2075.388 kb on - strandat 2075.481 kb on - strandat 2075.481 kb on - strandat 2075.481 kb on - strandat 2075.515 kb on + strandat 2075.586 kb on - strandat 2075.699 kb on + strand, within Pf6N2E2_1757at 2075.700 kb on - strand, within Pf6N2E2_1757at 2075.748 kb on + strand, within Pf6N2E2_1757at 2075.786 kb on - strand, within Pf6N2E2_1757at 2075.841 kb on + strand, within Pf6N2E2_1757at 2075.994 kb on + strand, within Pf6N2E2_1757at 2075.994 kb on + strand, within Pf6N2E2_1757at 2076.120 kb on + strand, within Pf6N2E2_1757at 2076.120 kb on + strand, within Pf6N2E2_1757at 2076.121 kb on - strand, within Pf6N2E2_1757at 2076.123 kb on + strand, within Pf6N2E2_1757at 2076.123 kb on + strand, within Pf6N2E2_1757at 2076.123 kb on + strand, within Pf6N2E2_1757at 2076.123 kb on + strand, within Pf6N2E2_1757at 2076.124 kb on - strand, within Pf6N2E2_1757at 2076.376 kb on + strand, within Pf6N2E2_1757at 2076.377 kb on - strand, within Pf6N2E2_1757at 2076.377 kb on - strand, within Pf6N2E2_1757at 2076.377 kb on - strand, within Pf6N2E2_1757at 2076.492 kb on + strand, within Pf6N2E2_1757at 2076.493 kb on - strand, within Pf6N2E2_1757at 2076.493 kb on - strand, within Pf6N2E2_1757at 2076.495 kb on - strand, within Pf6N2E2_1757at 2076.801 kb on - strand, within Pf6N2E2_1757at 2077.037 kb on + strandat 2077.038 kb on - strandat 2077.376 kb on - strand, within Pf6N2E2_1758at 2077.450 kb on - strand, within Pf6N2E2_1758at 2077.558 kb on + strandat 2077.558 kb on + strandat 2077.572 kb on + strandat 2077.867 kb on + strand, within Pf6N2E2_1759at 2077.868 kb on - strand, within Pf6N2E2_1759

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,074,628 - +0.5
2,074,628 - +0.1
2,074,755 - Pf6N2E2_1756 0.18 -0.5
2,074,887 - Pf6N2E2_1756 0.35 +1.0
2,074,887 - Pf6N2E2_1756 0.35 -0.2
2,074,910 + Pf6N2E2_1756 0.38 -0.2
2,074,911 - Pf6N2E2_1756 0.38 -0.8
2,074,911 - Pf6N2E2_1756 0.38 +1.0
2,074,911 - Pf6N2E2_1756 0.38 -0.4
2,074,911 - Pf6N2E2_1756 0.38 -1.1
2,074,953 - Pf6N2E2_1756 0.43 +1.0
2,075,093 + Pf6N2E2_1756 0.60 -3.1
2,075,230 + Pf6N2E2_1756 0.77 -0.5
2,075,231 - Pf6N2E2_1756 0.77 -0.1
2,075,231 - Pf6N2E2_1756 0.77 -0.7
2,075,270 + Pf6N2E2_1756 0.82 -0.6
2,075,270 + Pf6N2E2_1756 0.82 +1.7
2,075,270 + Pf6N2E2_1756 0.82 -0.5
2,075,271 - Pf6N2E2_1756 0.82 +0.0
2,075,288 - Pf6N2E2_1756 0.84 -0.1
2,075,292 - Pf6N2E2_1756 0.85 -0.8
2,075,318 + Pf6N2E2_1756 0.88 +0.1
2,075,388 - +1.7
2,075,481 - -0.1
2,075,481 - +0.5
2,075,481 - +0.2
2,075,515 + -0.3
2,075,586 - -1.7
2,075,699 + Pf6N2E2_1757 0.12 +3.1
2,075,700 - Pf6N2E2_1757 0.12 -0.0
2,075,748 + Pf6N2E2_1757 0.15 +2.2
2,075,786 - Pf6N2E2_1757 0.18 +0.4
2,075,841 + Pf6N2E2_1757 0.21 -1.4
2,075,994 + Pf6N2E2_1757 0.31 -0.2
2,075,994 + Pf6N2E2_1757 0.31 +0.5
2,076,120 + Pf6N2E2_1757 0.40 -1.7
2,076,120 + Pf6N2E2_1757 0.40 +0.4
2,076,121 - Pf6N2E2_1757 0.40 -0.2
2,076,123 + Pf6N2E2_1757 0.40 +1.0
2,076,123 + Pf6N2E2_1757 0.40 +1.3
2,076,123 + Pf6N2E2_1757 0.40 +0.7
2,076,123 + Pf6N2E2_1757 0.40 +0.1
2,076,124 - Pf6N2E2_1757 0.40 +0.5
2,076,376 + Pf6N2E2_1757 0.57 -0.5
2,076,377 - Pf6N2E2_1757 0.57 +0.4
2,076,377 - Pf6N2E2_1757 0.57 +0.5
2,076,377 - Pf6N2E2_1757 0.57 +0.1
2,076,492 + Pf6N2E2_1757 0.64 +4.2
2,076,493 - Pf6N2E2_1757 0.64 -0.7
2,076,493 - Pf6N2E2_1757 0.64 +0.1
2,076,495 - Pf6N2E2_1757 0.65 +0.4
2,076,801 - Pf6N2E2_1757 0.85 +0.5
2,077,037 + -1.2
2,077,038 - +0.0
2,077,376 - Pf6N2E2_1758 0.58 +0.2
2,077,450 - Pf6N2E2_1758 0.74 +0.3
2,077,558 + -2.4
2,077,558 + +3.1
2,077,572 + -1.1
2,077,867 + Pf6N2E2_1759 0.48 +1.8
2,077,868 - Pf6N2E2_1759 0.48 -0.2

Or see this region's nucleotide sequence