Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1755 and Pf6N2E2_1756 are separated by 73 nucleotides Pf6N2E2_1756 and Pf6N2E2_1757 are separated by 106 nucleotides Pf6N2E2_1757 and Pf6N2E2_1758 are separated by 83 nucleotides Pf6N2E2_1758 and Pf6N2E2_1759 are separated by 8 nucleotides
Pf6N2E2_1755 - 2-Oxobutyrate oxidase, putative, at 2,073,481 to 2,074,533
_1755
Pf6N2E2_1756 - Arginine ABC transporter, periplasmic arginine-binding protein ArtJ, at 2,074,607 to 2,075,413
_1756
Pf6N2E2_1757 - glutamine ABC transporter ATP-binding component, at 2,075,520 to 2,077,028
_1757
Pf6N2E2_1758 - Transcriptional regulator, MarR family, at 2,077,112 to 2,077,567
_1758
Pf6N2E2_1759 - uncharacterized domain 1, at 2,077,576 to 2,078,187
_1759
Position (kb)
2075
2076
2077
2078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2074.628 kb on - strand at 2074.628 kb on - strand at 2074.755 kb on - strand, within Pf6N2E2_1756 at 2074.887 kb on - strand, within Pf6N2E2_1756 at 2074.887 kb on - strand, within Pf6N2E2_1756 at 2074.910 kb on + strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.911 kb on - strand, within Pf6N2E2_1756 at 2074.953 kb on - strand, within Pf6N2E2_1756 at 2075.093 kb on + strand, within Pf6N2E2_1756 at 2075.230 kb on + strand, within Pf6N2E2_1756 at 2075.231 kb on - strand, within Pf6N2E2_1756 at 2075.231 kb on - strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.270 kb on + strand, within Pf6N2E2_1756 at 2075.271 kb on - strand, within Pf6N2E2_1756 at 2075.288 kb on - strand, within Pf6N2E2_1756 at 2075.292 kb on - strand, within Pf6N2E2_1756 at 2075.318 kb on + strand, within Pf6N2E2_1756 at 2075.388 kb on - strand at 2075.481 kb on - strand at 2075.481 kb on - strand at 2075.481 kb on - strand at 2075.515 kb on + strand at 2075.586 kb on - strand at 2075.699 kb on + strand, within Pf6N2E2_1757 at 2075.700 kb on - strand, within Pf6N2E2_1757 at 2075.748 kb on + strand, within Pf6N2E2_1757 at 2075.786 kb on - strand, within Pf6N2E2_1757 at 2075.841 kb on + strand, within Pf6N2E2_1757 at 2075.994 kb on + strand, within Pf6N2E2_1757 at 2075.994 kb on + strand, within Pf6N2E2_1757 at 2076.120 kb on + strand, within Pf6N2E2_1757 at 2076.120 kb on + strand, within Pf6N2E2_1757 at 2076.121 kb on - strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.123 kb on + strand, within Pf6N2E2_1757 at 2076.124 kb on - strand, within Pf6N2E2_1757 at 2076.376 kb on + strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.377 kb on - strand, within Pf6N2E2_1757 at 2076.492 kb on + strand, within Pf6N2E2_1757 at 2076.493 kb on - strand, within Pf6N2E2_1757 at 2076.493 kb on - strand, within Pf6N2E2_1757 at 2076.495 kb on - strand, within Pf6N2E2_1757 at 2076.801 kb on - strand, within Pf6N2E2_1757 at 2077.037 kb on + strand at 2077.038 kb on - strand at 2077.376 kb on - strand, within Pf6N2E2_1758 at 2077.450 kb on - strand, within Pf6N2E2_1758 at 2077.558 kb on + strand at 2077.558 kb on + strand at 2077.572 kb on + strand at 2077.867 kb on + strand, within Pf6N2E2_1759 at 2077.868 kb on - strand, within Pf6N2E2_1759
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,074,628 - +0.5 2,074,628 - +0.1 2,074,755 - Pf6N2E2_1756 0.18 -0.5 2,074,887 - Pf6N2E2_1756 0.35 +1.0 2,074,887 - Pf6N2E2_1756 0.35 -0.2 2,074,910 + Pf6N2E2_1756 0.38 -0.2 2,074,911 - Pf6N2E2_1756 0.38 -0.8 2,074,911 - Pf6N2E2_1756 0.38 +1.0 2,074,911 - Pf6N2E2_1756 0.38 -0.4 2,074,911 - Pf6N2E2_1756 0.38 -1.1 2,074,953 - Pf6N2E2_1756 0.43 +1.0 2,075,093 + Pf6N2E2_1756 0.60 -3.1 2,075,230 + Pf6N2E2_1756 0.77 -0.5 2,075,231 - Pf6N2E2_1756 0.77 -0.1 2,075,231 - Pf6N2E2_1756 0.77 -0.7 2,075,270 + Pf6N2E2_1756 0.82 -0.6 2,075,270 + Pf6N2E2_1756 0.82 +1.7 2,075,270 + Pf6N2E2_1756 0.82 -0.5 2,075,271 - Pf6N2E2_1756 0.82 +0.0 2,075,288 - Pf6N2E2_1756 0.84 -0.1 2,075,292 - Pf6N2E2_1756 0.85 -0.8 2,075,318 + Pf6N2E2_1756 0.88 +0.1 2,075,388 - +1.7 2,075,481 - -0.1 2,075,481 - +0.5 2,075,481 - +0.2 2,075,515 + -0.3 2,075,586 - -1.7 2,075,699 + Pf6N2E2_1757 0.12 +3.1 2,075,700 - Pf6N2E2_1757 0.12 -0.0 2,075,748 + Pf6N2E2_1757 0.15 +2.2 2,075,786 - Pf6N2E2_1757 0.18 +0.4 2,075,841 + Pf6N2E2_1757 0.21 -1.4 2,075,994 + Pf6N2E2_1757 0.31 -0.2 2,075,994 + Pf6N2E2_1757 0.31 +0.5 2,076,120 + Pf6N2E2_1757 0.40 -1.7 2,076,120 + Pf6N2E2_1757 0.40 +0.4 2,076,121 - Pf6N2E2_1757 0.40 -0.2 2,076,123 + Pf6N2E2_1757 0.40 +1.0 2,076,123 + Pf6N2E2_1757 0.40 +1.3 2,076,123 + Pf6N2E2_1757 0.40 +0.7 2,076,123 + Pf6N2E2_1757 0.40 +0.1 2,076,124 - Pf6N2E2_1757 0.40 +0.5 2,076,376 + Pf6N2E2_1757 0.57 -0.5 2,076,377 - Pf6N2E2_1757 0.57 +0.4 2,076,377 - Pf6N2E2_1757 0.57 +0.5 2,076,377 - Pf6N2E2_1757 0.57 +0.1 2,076,492 + Pf6N2E2_1757 0.64 +4.2 2,076,493 - Pf6N2E2_1757 0.64 -0.7 2,076,493 - Pf6N2E2_1757 0.64 +0.1 2,076,495 - Pf6N2E2_1757 0.65 +0.4 2,076,801 - Pf6N2E2_1757 0.85 +0.5 2,077,037 + -1.2 2,077,038 - +0.0 2,077,376 - Pf6N2E2_1758 0.58 +0.2 2,077,450 - Pf6N2E2_1758 0.74 +0.3 2,077,558 + -2.4 2,077,558 + +3.1 2,077,572 + -1.1 2,077,867 + Pf6N2E2_1759 0.48 +1.8 2,077,868 - Pf6N2E2_1759 0.48 -0.2
Or see this region's nucleotide sequence