Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_154 and Pf6N2E2_155 are separated by 198 nucleotides Pf6N2E2_155 and Pf6N2E2_156 are separated by 190 nucleotides Pf6N2E2_156 and Pf6N2E2_157 are separated by 247 nucleotides Pf6N2E2_157 and Pf6N2E2_158 are separated by 63 nucleotides
Pf6N2E2_154 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 209,411 to 210,793
_154
Pf6N2E2_155 - Probable transmembrane protein, at 210,992 to 211,447
_155
Pf6N2E2_156 - Carbon storage regulator, at 211,638 to 211,832
_156
Pf6N2E2_157 - hypothetical protein, at 212,080 to 212,445
_157
Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198
_158
Position (kb) 211
212 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 210.791 kb on + strand at 210.791 kb on + strand at 210.791 kb on + strand at 210.792 kb on - strand at 210.792 kb on - strand at 210.792 kb on - strand at 210.801 kb on + strand at 210.801 kb on + strand at 210.802 kb on - strand at 210.802 kb on - strand at 210.802 kb on - strand at 210.877 kb on - strand at 210.941 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.951 kb on + strand at 211.078 kb on - strand, within Pf6N2E2_155 at 211.211 kb on + strand, within Pf6N2E2_155 at 211.211 kb on + strand, within Pf6N2E2_155 at 211.212 kb on - strand, within Pf6N2E2_155 at 211.215 kb on - strand, within Pf6N2E2_155 at 211.477 kb on + strand at 211.477 kb on + strand at 211.503 kb on + strand at 211.518 kb on + strand at 211.518 kb on + strand at 211.519 kb on - strand at 211.567 kb on + strand at 211.683 kb on - strand, within Pf6N2E2_156 at 211.797 kb on + strand, within Pf6N2E2_156 at 211.797 kb on + strand, within Pf6N2E2_156 at 211.848 kb on + strand at 211.848 kb on + strand at 211.848 kb on + strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.853 kb on + strand at 211.853 kb on + strand at 211.853 kb on + strand at 211.854 kb on - strand at 211.854 kb on - strand at 211.854 kb on - strand at 211.855 kb on + strand at 211.855 kb on + strand at 211.856 kb on - strand at 211.896 kb on - strand at 211.896 kb on - strand at 211.897 kb on + strand at 211.897 kb on + strand at 211.897 kb on + strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.903 kb on - strand at 211.903 kb on - strand at 211.908 kb on + strand at 211.908 kb on + strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.921 kb on - strand at 211.921 kb on - strand at 211.955 kb on + strand at 211.955 kb on + strand at 211.975 kb on + strand at 211.976 kb on - strand at 211.976 kb on - strand at 211.976 kb on - strand at 211.976 kb on - strand at 212.044 kb on + strand at 212.045 kb on - strand at 212.108 kb on + strand at 212.634 kb on - strand, within Pf6N2E2_158 at 212.670 kb on - strand, within Pf6N2E2_158 at 212.684 kb on + strand, within Pf6N2E2_158 at 212.684 kb on + strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.691 kb on - strand, within Pf6N2E2_158 at 212.691 kb on - strand, within Pf6N2E2_158
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 210,791 + +0.9 210,791 + -0.7 210,791 + -0.7 210,792 - +0.9 210,792 - -0.2 210,792 - +1.1 210,801 + -0.2 210,801 + -1.4 210,802 - -1.1 210,802 - -2.0 210,802 - -1.7 210,877 - -1.7 210,941 + -1.3 210,948 + +0.0 210,948 + -1.0 210,948 + -1.2 210,948 + -2.9 210,948 + +1.2 210,948 + -1.3 210,948 + -0.3 210,948 + -0.4 210,948 + -1.3 210,948 + -1.1 210,949 - -1.7 210,949 - -1.2 210,949 - -1.2 210,949 - -1.1 210,949 - -1.0 210,949 - +0.0 210,949 - +0.1 210,949 - +0.2 210,949 - -0.9 210,951 + -0.9 211,078 - Pf6N2E2_155 0.19 +0.6 211,211 + Pf6N2E2_155 0.48 -1.8 211,211 + Pf6N2E2_155 0.48 +0.5 211,212 - Pf6N2E2_155 0.48 -0.8 211,215 - Pf6N2E2_155 0.49 -0.4 211,477 + -0.9 211,477 + -1.2 211,503 + -1.5 211,518 + -1.7 211,518 + -1.4 211,519 - -2.1 211,567 + -0.2 211,683 - Pf6N2E2_156 0.23 -1.6 211,797 + Pf6N2E2_156 0.82 -3.6 211,797 + Pf6N2E2_156 0.82 -1.4 211,848 + +0.1 211,848 + -1.2 211,848 + -0.8 211,849 - -1.8 211,849 - -1.1 211,849 - -1.1 211,849 - -1.5 211,853 + -2.5 211,853 + -2.6 211,853 + -2.4 211,854 - -0.1 211,854 - +4.9 211,854 - -0.3 211,855 + -2.0 211,855 + -0.2 211,856 - +1.1 211,896 - +1.5 211,896 - +2.4 211,897 + -1.2 211,897 + -0.4 211,897 + -1.2 211,898 - +0.4 211,898 - +1.0 211,898 - +1.2 211,898 - +1.3 211,898 - +2.0 211,898 - +1.1 211,898 - -0.8 211,898 - +0.7 211,903 - +1.0 211,903 - +1.4 211,908 + -3.3 211,908 + -2.9 211,909 - +0.8 211,909 - +1.3 211,909 - +2.9 211,909 - +0.2 211,909 - +1.5 211,909 - +1.3 211,921 - +2.2 211,921 - +1.5 211,955 + +0.2 211,955 + -1.2 211,975 + +0.3 211,976 - +0.4 211,976 - +1.1 211,976 - +1.8 211,976 - +1.5 212,044 + -1.4 212,045 - +1.4 212,108 + -0.7 212,634 - Pf6N2E2_158 0.18 -2.2 212,670 - Pf6N2E2_158 0.23 +1.3 212,684 + Pf6N2E2_158 0.25 -0.4 212,684 + Pf6N2E2_158 0.25 -0.4 212,685 - Pf6N2E2_158 0.26 +0.8 212,685 - Pf6N2E2_158 0.26 -0.3 212,685 - Pf6N2E2_158 0.26 -1.0 212,690 + Pf6N2E2_158 0.26 -0.2 212,690 + Pf6N2E2_158 0.26 +0.6 212,690 + Pf6N2E2_158 0.26 -1.3 212,690 + Pf6N2E2_158 0.26 -0.6 212,690 + Pf6N2E2_158 0.26 +0.9 212,691 - Pf6N2E2_158 0.26 -1.3 212,691 - Pf6N2E2_158 0.26 -0.1
Or see this region's nucleotide sequence