Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_14 and Pf6N2E2_15 are separated by 201 nucleotides Pf6N2E2_15 and Pf6N2E2_16 are separated by 202 nucleotides
Pf6N2E2_14 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 43,210 to 44,580
_14
Pf6N2E2_15 - Pyoverdine biosynthesis related protein PvdP, at 44,782 to 46,380
_15
Pf6N2E2_16 - hypothetical protein, at 46,583 to 46,909
_16
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 43.803 kb on + strand, within Pf6N2E2_14 at 43.991 kb on + strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14 at 44.235 kb on + strand, within Pf6N2E2_14 at 44.236 kb on - strand, within Pf6N2E2_14 at 44.633 kb on - strand at 44.686 kb on + strand at 44.688 kb on + strand at 44.689 kb on - strand at 44.714 kb on + strand at 44.751 kb on + strand at 44.751 kb on + strand at 44.751 kb on + strand at 44.824 kb on + strand at 44.824 kb on + strand at 44.824 kb on + strand at 44.827 kb on + strand at 44.828 kb on - strand at 45.044 kb on - strand, within Pf6N2E2_15 at 45.179 kb on - strand, within Pf6N2E2_15 at 45.179 kb on - strand, within Pf6N2E2_15 at 45.342 kb on + strand, within Pf6N2E2_15 at 45.342 kb on + strand, within Pf6N2E2_15 at 45.736 kb on + strand, within Pf6N2E2_15 at 45.775 kb on + strand, within Pf6N2E2_15 at 45.776 kb on - strand, within Pf6N2E2_15 at 45.776 kb on - strand, within Pf6N2E2_15 at 45.979 kb on + strand, within Pf6N2E2_15 at 46.139 kb on + strand, within Pf6N2E2_15 at 46.140 kb on - strand, within Pf6N2E2_15 at 46.285 kb on - strand at 46.348 kb on + strand at 46.401 kb on - strand at 46.831 kb on - strand, within Pf6N2E2_16 at 46.914 kb on + strand at 46.921 kb on + strand at 46.921 kb on + strand at 46.921 kb on + strand at 46.922 kb on - strand at 46.922 kb on - strand at 46.922 kb on - strand at 46.922 kb on - strand at 46.922 kb on - strand at 46.924 kb on + strand at 46.924 kb on + strand at 46.924 kb on + strand at 46.927 kb on + strand at 46.927 kb on + strand at 46.928 kb on - strand at 46.928 kb on - strand at 47.001 kb on + strand at 47.002 kb on - strand at 47.070 kb on + strand at 47.070 kb on + strand at 47.070 kb on + strand at 47.070 kb on + strand at 47.094 kb on + strand at 47.094 kb on + strand at 47.094 kb on + strand at 47.145 kb on + strand at 47.146 kb on - strand at 47.193 kb on + strand at 47.193 kb on + strand at 47.194 kb on - strand at 47.194 kb on - strand at 47.215 kb on + strand at 47.234 kb on + strand at 47.234 kb on + strand at 47.235 kb on - strand at 47.235 kb on - strand at 47.235 kb on - strand at 47.273 kb on - strand at 47.280 kb on + strand at 47.281 kb on - strand at 47.309 kb on - strand at 47.353 kb on - strand at 47.370 kb on + strand at 47.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 43,803 + Pf6N2E2_14 0.43 -0.8 43,991 + Pf6N2E2_14 0.57 -0.3 43,992 - Pf6N2E2_14 0.57 -2.9 43,992 - Pf6N2E2_14 0.57 +1.1 43,992 - Pf6N2E2_14 0.57 +1.3 44,135 + Pf6N2E2_14 0.67 +0.1 44,135 + Pf6N2E2_14 0.67 -0.2 44,235 + Pf6N2E2_14 0.75 +0.3 44,236 - Pf6N2E2_14 0.75 -0.6 44,633 - -0.5 44,686 + +1.6 44,688 + -0.1 44,689 - -0.7 44,714 + +0.8 44,751 + +1.8 44,751 + +0.3 44,751 + -1.9 44,824 + -2.0 44,824 + +0.3 44,824 + -0.4 44,827 + -1.4 44,828 - +1.8 45,044 - Pf6N2E2_15 0.16 +0.5 45,179 - Pf6N2E2_15 0.25 +0.1 45,179 - Pf6N2E2_15 0.25 +0.9 45,342 + Pf6N2E2_15 0.35 -0.8 45,342 + Pf6N2E2_15 0.35 -2.1 45,736 + Pf6N2E2_15 0.60 +0.7 45,775 + Pf6N2E2_15 0.62 +0.0 45,776 - Pf6N2E2_15 0.62 -0.9 45,776 - Pf6N2E2_15 0.62 -0.1 45,979 + Pf6N2E2_15 0.75 -0.6 46,139 + Pf6N2E2_15 0.85 +0.1 46,140 - Pf6N2E2_15 0.85 -0.6 46,285 - +0.6 46,348 + +1.6 46,401 - -0.3 46,831 - Pf6N2E2_16 0.76 -1.0 46,914 + -1.0 46,921 + -0.0 46,921 + +0.8 46,921 + -0.9 46,922 - +0.2 46,922 - -1.0 46,922 - -0.4 46,922 - +0.1 46,922 - +10.1 46,924 + +0.3 46,924 + -0.1 46,924 + +0.5 46,927 + -1.0 46,927 + -0.7 46,928 - +6.1 46,928 - +2.1 47,001 + -1.2 47,002 - +1.4 47,070 + -1.8 47,070 + -0.3 47,070 + +2.1 47,070 + -0.7 47,094 + +0.5 47,094 + -1.5 47,094 + +0.8 47,145 + +1.2 47,146 - -0.4 47,193 + -1.1 47,193 + -0.3 47,194 - -0.1 47,194 - -0.0 47,215 + +0.1 47,234 + +3.4 47,234 + -0.1 47,235 - +1.6 47,235 - -0.3 47,235 - +1.9 47,273 - -1.1 47,280 + +0.0 47,281 - -0.1 47,309 - +0.6 47,353 - -0.1 47,370 + -1.7 47,373 + -0.3
Or see this region's nucleotide sequence